Diabetes mellitus is poly-genetic inherited disease, which is influenced by both genes and environment. The environment may increase the susceptibility of diabetes mellitus via epigenetic way. While those from the same paternal and maternal imprinted genes in the role of offspring are not identical. Adverse intrauterine environment can be regarded as an integral part of the environment, therefore, intrauterine growth retardation may cause fetal epigenetic changes, which are also called imprinted genes. This paper will introduce the latest researches of the diabetes mellitus or insulin resistance-related imprinted genes,for example,GLUT4, IGF-2/H19,PGC-1 α and so on, to explore the relevant therapeutic targets of diabetes mellitus or insulin resistance at the level of epigenetics.

Objective To study the correlation of leptin,insulin-like growth factor-1 (IGF-1) and adiponectin in fetal cord blood with intrauterine growth retardation (IUGR).Methods Sixty-one fetus with IUGR were enrolled into the observation group and eighty healty fetus were enrolled into the control group.Leptin,IGF-1 and adiponectin were detected.Results The levels of leptin,IGF-1 and adiponectin in the observation group were lower than those in the control group,there were significant differences between the two groups (t =11.239,11.680,13.640,all P ＜ 0.01).The body mass in the observation group was (2 157 ± 243) g,which had significantly positive correlation with leptin (r =0.274,P ＜ 0.05),IGF-1 (r =0.532,P ＜ 0.05) and adiponectin (r =0.539,P ＜ 0.05).Conclusion There was a close relationship in leptin,IGF-1 and adiponectin with IUGR.

Tumor marker research is a hot field of oncology.Previous studies of tumor markers such as placental alkaline phosphatase,alpha-fetoprotein,c-kit and CD30 and so on,lack sufficient sensitivity and specificity for the early diagnosis of germ cell tumor.Recent research demonstrated that octamer-binding transcription factor 4 (OCT4),sal-like gene 4 (SALL4) can be used as new germ cell tumor markers.This paper reviewed the research progress on the OCT4 and SALL4 in recent years,to provide the reference about the early diagnosis,tumor typing,condition estimation,prognosis and targeted therapy of germ cell tumors.

ObjectiveTo evaluate the clinical efficacy of foot therapy in intervening sleep disorder and improving sleep quality in breast cancer during chemotherapy.MethodTotally 120 eligible patients were randomized into a treatment group and a control group, 60 in each group. The treatment group was intervened by Chinese medicinal steaming washing plus acupoint application, while the control group was by conventional treatment. The effects on sleep quality and sleep duration were observed and compared. ResultThe total effective rate was 68.3% in the control group versus 90.0% in the treatment group, and the difference was statistically significant (P<0.05). The sleep quality, sleep duration, sleep disturbance, and Pittsburgh Sleep Quality Index (PSQI) score in the treatment group were more significantly improved than those in the control group after treatment (P<0.05).Conclusion Foot therapy can obviously improve the sleep quality of breast cancer patients during chemotherapy, and reduce the occurrence rate of sleep disorder during chemotherapy.

The ventricular myocardial lactic dehydrogenase (LDH) and succinic dehydrogenase (SDH) were histochemically studied in 34 rabbits which were divided into 3 groups—normal control, sham operation and hemorrhagic shock. Using microspectrophotometric quantitative analysis showed that LDH activity was somewhat increased while SDH activity was obviously decreased (P

AIM: To establish the optimum preparation process for Bupleurum dropping pill. METHODS: A series of signal tests have been studied for the choice of substrate、 its proportion of substrate to drug、 the dropping and cooling agent temperature and dropping speed to make average pill weight,weight variation and roundness up to grade. RESULTS: The best pills formatting process were a ratio of PEG4000 to PEG6000(3∶1). CONCLUSION: This preparation method is suitable for both the laboratory test and the industrial production for Bupleurum dropping pill.

The FactorⅫ(FXII or a-Ⅻa)in Cohn's FIV was adsorbed by diato- mite,and then the FXⅡ(or ?-Ⅻa) was activated into ?-Ⅻa(Hageman factor fragment,PKA,MW31000)by the co-adsorbed kallikrein with sub- sequent releasing it toabuffer solu- tion.Finally,the product was purified by DEAE-Sephadex A_(50).The activity of prepared PKA was 8 963IU/ml,and the preparation can lead to signifi- cant reduction in blood pressure in rats.

Objective To investigate the levels of serum tHcy and Lp(a)and their association with insulin resistance in patients with type 2 diabetes and CHD. Methods T2DM patients were divided into two groups with CHD and without CHD in addition to normal control.The fasting serum levels of tHcy,Lp(a),FPG and FIns were detected, ISI were calculated，and the correlations among them were analyzed. Results The serum levels of tHcy,Lp(a),FIns,FPG of T2DM plus CHD were significantly higher than those of T2DM without CHD and controls(P<0.01~0.05).ISI was significantly lower in T2DM cases with CHD than those of T2DM cases without CHD and controls(P<0.01). ISI was negatively correlated with tHcy and Lp(a)in T2DM plus CHD group(P<0.05)but not correlated with those in T2DM case without CHD group(P>0.05). Conclusions Detections of the serum levels of tHcy and Lp(a)of T2DM play significant roles in knowing patient condition,guiding clinical treatment and earlier preventing and treating vascular complication of diabetes.

Objective To analyze the changes in body size of Chinese newborns in nine cities during 1975-2005.Methods The data of weight,height and head circumference of newborns within 3 days after birth from four national physical growth surveys named “a national survey on growth of children under seven years of age in nine cities of China” in 1975,1985,1995 and 2005 were used.The investigation areas were urban and suburban areas of Beijing,Harbin and Xi'an in the northern part;Shanghai,Nanjing and Wuhan in the central part; Guangzhou,Fuzhou and Kunming in the southern part of China.Random cluster sampling method was used,7325,6660,7109 and 6144 healthy newborns were examined in 1975,1985,1995 and 2005,respectively.The changes of body size between 1975 and 2005,urban-suburban difference and regional difference were compared with u test,ANOVA or x2 test.Results(1)The average weight had increased during the past 30 years no matter boys and girls,from urban and suburban areas.The increments was 40 ～ 100 g.The percentiles of weight had also increased and the distribution had shifted noticeably towards heavier weights.The proportion of macrosomia had increased from 3.2％(212/6660)in 1985 to 4.3％(265/6144)in 2005.(2)The length of newborns remained unchanged over the 30-year period no matter boys and girls,from urban and suburban areas.Head circumference of newborns showed only a minor increase(0.1～0.3 cm).(3)Ponderal index(PI)had increased gradually from 1975 to 2005.The means of PI had increased from 25.4 kg/m3 in 1975 to 26.0 kg/m3 in 2005.(4)Body size of urban newborns was slightly bigger than that of suburban newborns.The urban-suburban difference of weight and height became smaller and smaller in the 30 years,while the urban-suburban difference in PI became slightly bigger in 2005.(5)Weight,height and head circumference of newborns in northern and central areas were higher than those of southern newborns and the regional difference had not significant changes in the 30 years.The PI of newborns in northern and central areas was higher than that of southern part in 1975,while the PI of southern part became the highest in 2005.Conclusions The body size of newborns became slightly bigger and the proportion of macrosomia increased between 1975 and 2005.The urban-suburban difference became smaller and the regional difference remained in the 30 years.