BACKGROUND:Previous clinical fol ow-up study showed that disc degeneration of adjacent segment after anterior cervical discectomy and fusion was faster than that of artificial cervical disc replacement. Compared with the anterior cervical discectomy and fusion, artificial cervical disc replacement can maintain a good range of motion of replacement segment. Further investigation should be taken to compare the difference between stress and fusion after replacement. OBJECTIVE:To compare the adjacent level discs loads between artificial cervical disc replacement and anterior cervical discectomy and fusion. METHODS:A healthy 30-year-old male volunteer was scanned with CT at the artificial cervical intervertebral disc and anterior cervical plate. Three-dimensional images were reconstructed with Mimics 10.01 and Geomagic Studio.v11 software. Above three-dimensional data were input into the Abaqus6.9 finite element analysis software for meshing, assignment, and stress analysis. Finite element method was used to simulate the stress changes of the adjacent segments after artificial cervical disc replacement and anterior cervical discectomy and fusion. RESULTS AND CONCLUSION:(1) Under the same preload, during anteflexion, posterior extension, and lateroflexion, the disc stress at adjacent segment was significantly larger after anterior cervical discectomy and fusion than normal disc. Compared with normal persons, no significant difference was detected in stress of adjacent segment at anteflexion, posterior extension, and lateroflexion after artificial cervical disc replacement. (2) Compared with artificial cervical disc replacement group, the stress of adjacent segment increased 10.3%-51.6%in the anterior cervical discectomy and fusion group. (3) Finite element analysis showed that the stress was larger in the anterior cervical discectomy and fusion group than in the artificial cervical disc replacement group. With prolonged fol ow-up, compared with the conventional anterior decompression and fusion, artificial cervical disc replacement can better play its protective effect on the adjacent intervertebral disc.

Objective To investigate the relationship between the Plasmodium malaria titre with the body temperature and prog-nosis to provide a theoretical basis for malaria diagnosis and missed diagnosis.Methods Ninety-three malaria test positive speci-mens were collected and simultaneously performed the plasmodium test by using the colloidal gold immune chromatography assay and thick film blood slide.The body temperature of malaria patients was recorded ,and the malaria test was conducted once when the body temperature was increased by 1 ℃ until positive for malaria detection.Results In 93 tested specimens ,the infection rate of Chinese was 56.98% (53/93) ,and which of foreigners was 43.01% (40/93) ,the difference between Chinese and foreigners malaria infection groups had no statistical significance ,showing that malaria infection had no relationship with race.Among 93 specimens , the initial malaria infection was in 48 cases ,including 47 Chinese and 1 foreigner ,and 45 cases had twice or more malaria infection , in which 33 cases had the titers of 1:10 ,with the recurrence rate of 0.09% (3/33) and the re-burning rate was 0.27% (9/33). The titer of 1:20 had 28 cases ,with the recurrence rate 0.04% (1/28) ,and the burning rate 0.21% (6/28).Titer of 1:30 was 19 people ,with the recurrence rate 0.16% (3/19) and the burning rate 0.37% (7/19).The titer of 1:40 was 13 people ,with the re-currence rate 0.15% (2/13) ,and the re-burning rate of 0.38% (5/13) ,there was no statistically significant difference between the number of recurrent and re-burning patients with the titre(χ2 =2.5 ,P>0.05 ;χ2 =1.96 ,P>0.05).Conclusion There is a positive correlation between malaria with the titer and temperature.The titer is elevated ,and the probability of recurrence and re-buring is increased.

Objective To investigate the role of Th17 cells and related cytokines in Helicobacter pylori(H.pylori) infection.Methods To establish a mouse model of H.pyloriinfectious gastritis.Meanwhile,a treatment group and control group were set up.The histological changes in the gastric mucosa were observed and scored under light microscopy;The expression of IL-17 and IL-23 mRNAs as well as protein in gastric tissue was detected by RT-PCR and ELISA,respectively;Th17 cells in mice splenocyte were evaluated using the flow cytometry (FCM) method.Results We found significantly higher levels of IL-17 and IL-23 protein and mRNA levels in supernatant of gastric tissue homogenates of infection group as compared to controls and Th17 cells in spleen from mice of H.pylori infection group were all obviously higher than that of control group;IL-17 mRNA,IL-23 mRNA and the ratio of Th17 cells in mice splenocyte of H.pylori infection group mice increased gradually with the time prolonged;The levels of both mRNA and protein levels of IL-17and IL-23 decreased significantly in the treatment group compared with pre-treatment;There was a positive correlation between IL-17 and IL-23 expression levels of mice gastric mucosa and gastric inflammation degree.Conclusion H.pylori infection induced the immune response of Th17 cells;The levels of IL-17 and IL-23 increased in gastric mucosa of mice after H.pyloriinfection;The degree of H.pylori-infected mice gastric inflammation was positive correlation with the levels of IL-17 and IL-23 of gastric mucosa.