Kawasaki disease is an acute and self-limited systemic vasculitis.Coronary artery lesions are the most common complications.The principal treatment with intravenous immunoglobulin and high doses of oral aspirin has significantly decreased the morbidity of coronary artery lesions.However, increased cases of IVIG non-responsiveness and refractory Kawasaki disease in recent years make the treatment more difficult.The existing progress indicates that infliximab provides a new therapeutic option for Kawasaki disease, especially for IVIG non-responsiveness and refractory Kawasaki disease.This paper reviews recent studies associated with application of infliximab in Kawasaki disease.

Objective To construct an adenovirus vector expressing small interfering RNA ( siRNA) targeting to rat Raf-1 gene and identify its function in cardiomyocytes.Methods The siRNA containing DNA sequence targeting to Raf-1 and its negative control sequence were designed, synthesized, annealed and subcloned into adenoviral shuttle vector pAdTrack-CMV.The recombinant adenovirus vector pAd-siRaf-1 was obtained by homologous recombination with pAdTrack-siRaf-1 linearized by PmeI and pAdeasy-1 in bacteria BJ5183, then transfected into HEK293 cells to package the adenovirus.Cardiomyocytes were infected with the adenovirus pAd-siRaf-1, and the expressions of Raf-1 and NF-κB protein were detected by Western blotting.[ 3 H ]-leu incorporation was evaluated by scintillation.The surface area of cardiomyocytes was measured using a HJ2000 image analysis system.Results The adenovirus vectors were verified by enzyme digestion and DNA sequencing.Compared with the Ang II group, Raf-1 and NF-κB expression, the surface area and [ 3 H]-leu incorporation of cardiomyocytes were significantly decreased in cardiomyocytes infected with the adenovirus PAd-siRaf-1.Conclusions A recombinant adenovirus vector containing rat siRaf-1 gene is successfully constructed.It can effectively reduce Raf-1 and NF-κB expression and cardiomyocyte hypertrophy induced by Ang II.

OBJECTIVE:To evaluate the economic effectiveness in different chemotherapeutic schemes for acute mononu?clear leukemia(M 5 ).METHODS:Using pharmacoeconomic cost-effectiveness analysis,three chemotherapy schemes for M 5 we_ re compared.RESULTS:Among three schemes,DAE scheme was the best one.CONCLUSION:The results show that phar?macoeconomics plays an important role in optimizing therapeutic scheme,in helping the physician to choose the best therapeutic plan in making clinical decision.

Objective To analyze the correlation between students' scores and master degree of knowledge points in the close examination of the unit introduction of nursing. Methods A classification decision tree model was used to analyze the relationship between students' achievement and knowledge points in 321 grade 2014 nursing students. Results There were 20 knowledge points in the paper, 7 of which had significant influence on scores; there were 27 nodes,16 of which were leaf nodes; the overall predicted percentage was 86.0%. Conclusions The total score is related to knowledge score; the total score of gifted students is higher;and for total score of medium students is medium;and for total score of poor is lower. Measures to reform teaching methods and evaluation scheme should be taken.

Traditional Chinese Medicine has its own academic thoughts and practical experience on diagnosing and treating phlegm disease,which has sprouted in Qin and Han Dynasties,originated in Jin and Tang Dynasties,developed in Song and Yuan Dynasties,become prosperous in the Ming and Qing Dynasties and made much progress in the recent years.This article shows researches of academic thoughts and clinic experiences on diagnosing and treating phlegm disease,which is based on ancient books and records of Traditional Chinese Medicine from Pre-Qin Period to the Sui and Tang Dynasties.Hope to provide beneficial reference to modern clinic,health preservation,prevention and treatment of phlegm disease.

During the Ming dynasty,great progress had been made on the theoretical and clinical aspects of diagnosis and treatment of phlegm disease in TCM.It mainly showed that: during this period,unprecedented monographs about phlegm diseases such as 《Tan Huo Zhuan Men》,《Tan Huo Dian Xue》 appeared.The ancient medical scholars all paid attention to the in? uence and harm of the phlegm to the body and accumulated abundant experience in clinical diagnosis and treatment,further made a great summary to provide reference to the posterity.In this article,several physicians’ academic thoughts of phlegm disease were chosen to research.The theories and experience of ancient medical scholars were explained.It has important theoretical and practical values for the health preservation and clinical diagnosis and treatment.

The physicians of Qing Dynasty made deeper knowledge to the formation and harm of the phlegm and systematically summarized the theory of the diagnosis, syndrome differentiation and treatment of the phlegm diseases. In the literatures of such as CHEN Shi-duo, LIN Pei-qin, HE Meng-yao, YOU Zai-jing, the special discussion of the phlegm diseases diagnosis and treatment which contents were theory, principle, prescription and drugs. Next, in the surgery, ophthalmology, gynecology, pediatrics, the physicians paid attention to the diagnosis and treatment from the phlegm and summarized the clinical practical experience. Moreover, in the Chuan Ya Nei Bian, Chuan Ya Wai Bian written by ZHAO Xue-min, the experience of "Zou Fang Yi" on treating the phlegm disease by the method of Ding, Chuan, Jie had its own characteristics.

This paper elucidates the diagnosis and treatment of phlegm syndrome in Jing-Yue Complete Works(Jing Yue Quan Shu from pathogenesis,syndrome differentiation,principle and method of treatment,recipe,medicines,diet and rest,which are used for reference in the modern clinical diagnosis and treatment of traditional Chinese medicine.The diagnosis and treatment of phlegm syndrome in Jing-Yue Complete Works(Jing Yue Quan Shu) is widely used in internal medicine,surgery,gynecology and pediatrics,etc.Based on the point of emphasizing yin-yang in person,Zhang Jingyue supposed that the diagnosis and treatment of phlegm syndrome should focus on spleen and kidney,strengthening the body resistance,treating symptoms in acute condition,choosing recipe and medicines according to the position and character of phlegm,diet and rest.

Objective To explore the role of ripply1 in zebrafish dorsal-ventral development .Methods Using ze-brafish whole-mount in situ hybridization to examine the ripply1 expression pattern in early embryo development .To analyse the expression pattern changes of dorsal-ventral marker genes at shield stage and the morphological changes at 24 hpf (hours post-fertilization) after overexpression of ripply1 by injecting synthetic mRNA at 1-cell stage.Using Tol2 transposon technology to obtain a ripply1 promoter driven GFP transgenic fish and to identify promoter region that recapitulates endoge -nous ripply1 expression pattern .Results The in situ hybridization results revealed that ripply1 specifically expresses in the future dorsal region at shield stage .Overexpression of ripply1 caused an enhanced expression of dorsal marker genes and a reduction of ventral marker genes .Embryos overexpressing ripply1 also showed severely dorsalized phenotype , with enlarged head, reduced ventral yolk extension , and shortened posterior trunk and tail regions , and the formation of a secondary trunk axis.Transgenic fish revealed the maternal expression of ripply1 and suggested that a 1.2 kb promoter-driven GFP is able to recapitulate the endogenous gene expression pattern .Conclusion ripply1 may participate in the early development of dor-sal-ventral axis in zebrafish embryo .

Objective To analyze the long- term results of vascularized fibular graft to repair defects of long bone. Methods Nineteen patients undergone vascularized fibular graft were followed- up for 5－ 20 years(mean 10.79 years). Limb function was graded with Enneking s system, and presented as percentage of normal limb function. The changes of the vascularized fibular graft were evaluated radiographically according to the International Symposium on Limb Salvage. Results The long term results of the vascularized fibular graft varied in different types of bone defects. Nine patients with congenital pseudoarthrosis of tibia had the limb function restored in 65％ and one had nonunion of fibular graft, 3 fractures(7 times), and 5 angular deformities. Three patients with congenital radial deficiency had 62％ restoration of the limb function. All of them showed early closure of epiphyses of the grafted fibulae. Five cases with osteomyelitis and bone defects combined soft tissue deficiency had 94％ restoration of the limb function, and only one occurred fracture of fibular graft. One case with soft tissue defect and concomitant ulnar defect, ulnar nerve and median nerve injury, 67％ function of limb was recovered, and union of fibula graft was good. 100％ function of limb was recovered in 1 case with cavernoma of radius and without occurring valgus deformity of donor site. Conclusion The vascularized fibular graft is a good method for repairing long segmental defects of bone. Combined with other tissue flap, the operation can reconstruct bone defect and fulfil the cover of soft tissue. For congenital tibia pseudoarthrosis and radial deficiency, the long- term results are not so satisfactory.