Objective To investigate the early diagnostic methods for sub-clinical hepatic encephalopathy (SHE).Methods 46 hepatocirrhosis patients without hepatic encephalopathy (SHE group) were recruited to perform P300, brainstem auditory evoked potential (BAEP), numeral connection teat (NCT),mini-mental state examination (MMSE)and electroencephalogram (EEG), compared with 43 unhepatopathy patients (control group) and within following up.Results The P300 delitescence and the NCT value in SHE group were obviously longer than those of the control group (allP

Objective The clinical significance of serum cytokine in kidney transplanted patients have been evaluated by observing the variation of cytokines and biochemical parameters. Methods The serum IL-1?、IL-1?、IL-2、IL-4、IL-6、IL-8、IL-10、VEGF、IFN-?、TNF-?、MCP-1、EGF and 22 items of biochemistry parameters have been followed in 10 patients during 90 days around operation time. Cytokine had been measured by biochips methods in evidence 180 automatic biochips analyzer. The serum biochemical parameters had been measured by Roche diagnostic company reagents kits in HITACHI 7600DDP analyzer.Results Serum IL-6 and IL-8 had reached the highest level [(42.5?42.8) ng/L and (343.5?493.1) ng/L] at 7 days after kidney transplantation,whereas IL-2 and IL-10 were reached the highest level [(16.3 ? 5.7) ng/L and (16.6 ? 24.2) ng/L] respectively at 14 days. Then they decreased gradually, but IL-6 and IL-8 were still higher than normal control. The decreased VEGF, EGF and MCP-1 in kidney failure patient had increased gradually after transplantation. But EGF was still lower than normal control at 90 days after operation. Urea、Cr、Ua and Glu had been restored to normal level after two month of operation and the significant relationship between IL-8 and serum LDH activity have been found( r=0.93,P

Objective To investigate the clinical therapeutic effects of neuromuscular electric stimulation (NMES) on patients with swallowing disorders after stroke. MethodsA total of 98 patients with a swallowing disorder after stroke were randomly divided into an NMES group (38 cases),a rehabilitation group (30 cases) and a control group (30 cases).In the control group the patients were treated with medication only; the in rehabilitation group they were treated with medication and swallowing rehabilitation training; in the NMES group they were treated with medication and NMES.The treatments lasted for 3 weeks in all groups.The swallowing function of patients in each group were observed and assessed with dysphagia scoring pre-and post-treatment.Complications of the swallowing disorders,side effects of treatment and the stomach tube leaving rate were observed. ResultsAfter treatment,the dysphagia scores of patients in the NMES and rehabilitation groups both increased,but the average scores in the NMES group were significantly higher than in the rehabilitation and control groups.The difference in therapeutic effect between the three groups was statistically significant.After treatment,the incidence of aspiration pneumonia in the NMES group was significantly lower than that in the control and rehabilitation groups.The average stomach tube leaving rate in the NMES group was significantly lower than that in the control group,but there was no significant difference in stomach tube leaving rates between the rehabilitation group and the NMES group.There was no significant difference among the three groups in malnutrition.No side effect was observed in any group.Conclusions NMES could alleviate post-stroke dysphagia and decrease the incidence of aspiration.The short-term therapeutic effect of NMES was better than that of swallowing rehabilitation training,but the long-term therapeutic effects were about the same in the two groups.

50% resolution of elevated ST-segment in 1h after PCI was increased in the L-CN treated group. LVEDD was less but LVEF was higher in the treatment group compared with the control group 3 months after PCI. Conclusion L-CN has significant myocardial protection effects in patients with AMI undergoing primary PCI, which is characterized by inhibition of the reperfusion injury , reduction of infarction area and improvement of left ventricular remodeling.

Objective To evaluate the clinical significance of the multiple cytokines in the serum of SARS patients and explore the rel ationship between the immune-reactivity and pathological damages. Methods 12 different serum cytokines have been detected in 4 groups ( inchoation, metaphase, convalescence of SARS patients and Healthy control) by using biochips technique(RANDOX) and to study the changes of each cytokine level in SARS patients. Results Compared with healthy group, IL-6 , IL-8 , IL-10 ,IFN? increased obviously and IL-1? ,IL-2,IL-4,VEGF,EGF,MCP-1, TNF?decreased obviously. Whereas IL-1?has no statistic changes among different stages of SARS. Conclusion There were obvious changes of multiple cytokines in different phase of SARS pathological process, especially in the early phase. It is further support the hypothesis that over-reaction of the immune system initiated the pathological injuries of the patients.

BACKGROUND:A series of inflammatory signal pathways are activated accompanied by increasing expressions of inflammatory factors after periodontal tissues being stimulated by exogenous substances like bacterium. Inflammation is closely related to periodontal tissue repair and regeneration.
OBJECTIVE:To il ustrate the correlation of immune response of periodontitis and periodontitis-related inflammatory cytokines to p38 mitogen activated protein kinase (p38MAPK) pathway, and to provide a new idea and method for the treatment of periodontitis in the future.
METHODS:The related literatures of periodontitis and p38MAPK pathway published from January 1990 to January 2016 were retrieved from the databases of CBM, CNKI, SWIC and PubMed. The research and progress of periodontal tissue repair and regeneration after periodontitis were analyzed.
RESULTS AND CONCLUSION:Total y 36 literatures were enrol ed final y. Inflammation-regulated and inflammation-associated signaling pathways as wel as subsequent expression of inflammatory mediators can partly control the excessive disease-induced inflammation and immune responses of the host, among which p38MAPK signaling pathway may be involved in the occurrence and development of periodontitis. More studies, however, are needed to validate these findings. The regulation of p38MAPK signaling pathway is stil of great significance in the treatment of periodontal disease, and we hope to provide a new insight and basis for clinical diagnosis and treatment of periodontitis.

OBJECTIVE:To observe clinical efficacy and safety of rivaroxaban in the treatment of pulmonary embolism. METHODS:100 patients with pulmonary embolism were selected and randomly divided into observation group and control group, with 50 cases in each group. Both groups were given nursing intervention for rehabilitation. Observation group was treated with riva-roxaban 15 mg,bid,for consecutive 3 weeks,3 weeks later adjusting to 20 mg,qd;control group was given low molecular weight heparin 1 mg/kg,bid,combined with warfarin sodium 5 mg,qd,for more than 5 days,stopped taking low molecular weight heparin until the INR reached the target 2-3 and given warfarin alone orally. Treatment course of 2 groups lasted for 3 months. Clinical efficacy of 2 groups were observed,and PaO2,PaCO2 and D-dimer levels were observed in 2 groups before and af-ter treatment;the improvement of clinical symptoms as cough,pectoralgia,dyspnea,and the incidence of ADR were recorded in 2 groups. RESULTS:The total effective rate of observation group (90.0%) was significantly higher than that of control group (74.0%),with statistical significance(P<0.05). After treatment,PaO2,PaCO2 and D-dimer levels of 2 groups were increased signif-icantly,compared with before treatment;the observation group was higher than the control group,with statistical significance(P<0.05). The remission rate of clinical symptoms as cough,pectoralgia,dyspnea in observation group was significantly higher than in control group,with statistical significance (P<0.05). The incidence of ADR in observation group (12.0%) was significantly lower than in control group (30.0%),with statistical significance (P<0.05). CONCLUSIONS:Rivaroxaban supplemented by reasonable nursing intervention can effectively improve clinical efficacy of patients with pulmonary embolism with low incidence of ADR.

The laboratory animal science, a comprehensive discipline researching on experimental animal and animal experimentation, is the life sciences research foundation and the support condition. It has very vital significance to sudy the related discipline especially the life sciences. This article discusses the construction of the status of Laboratory Animal Science course combined with the development of laboratory animal science and contacted with the characteristics of medical colleges and related disciplines.

BACKGROUND:Tracheal injury suture and the development of a new tracheal prosthesis all need to understand the tensile mechanical properties of tracheal cartilage.so as to repair and rebuild tracheal function.Previous researches on the biomechanics of artificial trachea are many reported.while the biomechanics of human tracheal cartilage is reported less.OBJECTIVE:To investigate the mechanical properties of tracheal cartilage using one-dimensional tensile test method.METHODS:Two fresh cadaver specimens of normal human trachea.with the informed consents of their families.were involved.The specimens were thaw at room temperature,and then tracheal cartilage specimens were cut using scalpel into 20 samples at the length of 25 mm,width 5 mm,thickness 1.8-2.2 mm.The 20 tracheal cartilage samples were subjected to one-dimensional tensile test with Shimadzu electronic universal testing machine Japan,at the tensile test speed of 5 mm/min.The tensile maximum load,maximum displacement,maximum stress,maximum strain,elastic modulus and stress-strain curve of the specimens were observed.RESULTS AND CONCLUSION:The maximum load of human cadaver tracheal cartilage was(60 946±10.377)N,maximumdisplacementwas(1.973±0.159)mm,maximum stresswas(6.229±1.125)Mpa,maximum strainwas(32.825±2.776)%.Tracheal cartilage stress-strain curves was changed along with the index,the initial low slope of the curye was due to the direction of imposed tension was similar with the arrangement of collagen structure,the steep slope represented the tensile strength of collagen.To describe the tracheal cartilage stress-strain relationship in a one-dimensional tension,15 stress-strain data of the tracheal cartilage experimental data adopted polynomial by the least square fitting method,to obtain stress(δ)-strain(ε)relationship forrnula:σ(ε)=-0.111 3e~5+1.602 1e~4-7.821 6e~3+17.995 1e~2+3.624e.It is indicated that tracheal cartilage has a strong capacity to bear Joad and resist deformation,reflecting their viscoelastic mechanical properties of both flexibility and viscosity,supporting that the mechanical properties of cartilage is positively related to cartilage collagen content.