1.An analysis of iron-load in 443 cases of thalassemia with different gene types
Huaqiong CHEN ; Bing DENG ; Yaping YOU
Journal of Third Military Medical University 2003;0(20):-
005), while iron overload occurred in 8 of 57 cases of ?-thalassemia (14.0%) and 89 out of 386 cases of ?-thalassemia (23.1%). Although the occurrence rate of iron overload in ?-thalassemia appeared higher than that in ?-thalassemia, no significant difference existed. Homozygote ?-thalassemia was prone to iron overload, followed by non-deletion HbH diseases, at the occurrence rate of 79.2% and 27.3% respectively. Iron deficiency was inclined to be associated with silent ?-thalassemia at the occurrence rate of 45.5%, followed by heterozygote ?-thalassemia (30.3%). Conclusion Both iron overload and iron deficiency can be found in thalassemia. Therefore, special treatments should be carried out according to given cases.
2.Effect of oxygen tension on chondrocytic phenotype in vitro
Jing LIANG ; Lianfu DENG ; Yaping ZHU
Orthopedic Journal of China 2006;0(23):-
[Objective] To examine the specific gene and protein expression of primary and passaged chondrocytes in normal oxygen tension and hypoxia.[Methods]Articular chondrocytes were isolated from limb joint cartilage of C57BL/6 mice(3~5 days).Primary chondrocytes(P0)and passaled chondrocytes(P1,P2)were cultured in normal oxygen tension and hypoxia respectively for two days.The mRNA levels of collagen II,aggrecan,sox9,Indian hedgehog(ihh),parathyroid hormone-related protein(PTHrP),bone morphogenetic protein(BMP)-4 and wnt5a were examined with RT-PCR,and protein levels of collagen II,aggrecan were examined with immunohistochemistry and toluidine blue staining.[Results]During the culture of primary chondrocyte,the expression of collagen II and aggrecan increased under hypoxia,mRNA levels of collagen II,wnt5a increased and ihh decreased at the same time.The mRNA levels of special genes and differentiation related genes of passaged chondrocytes were not altered under hypoxia.[Conclusion]Protein and mRNA level of Collagen II of primary chondrocyte under hypoxia increased.It may be regulated by chondrocyte differentiation gene ihh,and wnt5a.The chondrocyte phenotype could not be resumed under hypoxia through short-term monolayer culture in vitro.
3.A case report of β-ketothiolase deficiency
Tian TAN ; Yaping DENG ; Lin KONG
Chinese Journal of Applied Clinical Pediatrics 2021;36(8):623-625
The clinical data of a child diagnosed with β-ketothiolase deficiency in Children′s Hospital of Chongqing Medical University in September 2018 were reviewed, and nutritional intervention and follow-up were carried out.The male child was 1 year and 4 months old, and he had clinical manifestations, including vomiting, diarrhea, mental depression and repeated metabolic acid poisoning.Gene detection: heterozygous variation of ACAT1 gene c. 401T>C (p.M134T) and c. 481T > C (p.Y161H). This study was mainly aimed at children with β-ketothiolase deficiency for nutritional treatment of individualized nutrition therapy, suggests that energy supply should be guaranteed and protein and fat intake should be limited in acute stage, and protein and fat intake should be increased gradually in remission stage, which can not only ensure growth and development, but also reduce the occurrence of metabolic crisis.
4.Effects of rat serum containing Chinese herbal medicine Sangen Decoction on osteoclastogenesis and bone resorption of osteoclasts induced by polymethylmethacrylate particles.
Shuqiang WANG ; Wu RAO ; Lianfu DENG ; Yaping ZHU ; Yongqiang CHEN
Journal of Integrative Medicine 2011;9(1):64-9
To investigate the effects of Sangen Decoction, a compound Chinese herbal medicine, on osteoclastogenesis and bone resorption function of osteoclasts induced by polymethylmethacrylate particles in vitro.
5.Culture of human osteoclasts.
Guilin OUYANG ; Qingming YANG ; Lianfu DENG ; Fuping XU ; Yaping ZHU
Journal of Integrative Medicine 2003;1(1):57-61
To establish a stable, useful culture system for human osteoclasts and to investigate the effect of osteoblasts on the differentiation, proliferation and activation of osteoclasts so as to provide a base for the studies on prevention and treatment of osteolysis and osteoporosis.
6.Study on estrogen receptor of adult human osteoblasts
Guilin OUYANG ; Qingming YANG ; Lianfu DENG ; Fuping XU ; Yaping ZHU
Journal of Integrative Medicine 2003;1(2):132-4
OBJECTIVE: To examine estrogen receptor (ER) in osteoblasts from adult human and to elucidate the mechanism of estrogen in modulating bone metabolism. METHODS: The cultured osteoblasts were harvested from bone chips by modified sequential digestive enzyme release and immunohistochemical assay of ER in osteoblasts were carried out in three groups of female adults: normal control (group 1), patients with moderate osteoporosis (group 2) and patients with serious osteoporosis (group 3). The percentages of ER-positive osteoblasts from the three groups were compared by t test. RESULTS: The brown marks that indicate ER were found in nuclei and plasma of the osteoblasts, and the percentages of ER-positive osteoblasts among three groups were significantly different. CONCLUSION: ERs exist in nuclei and plasma of the osteoblasts. Estrogen may modulate bone metabolism through binding ER in nuclei and plasma of the osteoblasts. The reduction of ER of osteoblasts may play an important role in the pathogenesis of postmenopausal osteoporosis.
7.The effects of micro alcohol on the serum enzyme activities in vivo and vitro
Zhennan DONG ; Heping ZHOU ; Hongying LIU ; Xinxin DENG ; Yaping TIAN
Chinese Journal of Laboratory Medicine 2001;0(04):-
Objective To investigate the effects of micro alcohol on serum enzymes in vitro and vitro Methods Serum alcohol and ALT?AST?GGT?ALP?CK?LDH?AMY?LIPA activities were measured before and after alcohol consuming (1 ml/kg) in 14 volunteers Meanwhile, the direct inhibitory effects of alcohol on the serum enzymes were studied by comparing the serum enzyme activities with or without alcohol Results Alcohol consuming could depress the serum AST activity from (24 04?3 66) U/L to (22 25?3 27) U/L and LIPA activities from (155 86?93 51) U/L to (128 35?84 85) U/L, whereas increase the other serum enzyme activities, but only serum AMY were found statistic difference [from (48 78?10 66) U/L to (55 50?12 60) U/L] The inhibitory effects of alcohol on all the measured enzymes were found in vitro studies Conclusions Alcohol could obvious influence the serum enzyme activities both in vivo and vitro Avoiding the contamination of alcohol during sample collection and routine laboratory work is necessary
8.GRAPHS INVESTIGATION ABOUT EYSENCK PERSONALITY QUESTIONNAIRE REVISED IN CHINA FOR 302 CASES OF MEDICAL STUDENTS AND MENTAL INTERVENTION STRATEGY
Yaping WANG ; Yuan TIAN ; Ling LIU ; Xinxin MA ; Yongning DENG
Chinese Medical Ethics 1995;0(04):-
To understand the personality characteristics and temperament type of medical students in Xi'an Jiao tong University,and to provide scientific evidence for their targeted mental intervention by analyzing graphs about Eysenck personality questionnaire revised in China.,310 medical students were investigated by using EPQ(adult version).We found that there were more male students with typical psychoticism personality than female students with the same personality,and there was statistical significant difference in the distribution of the type of temperament between male and female students.Medical students with stable personality probably accounted for two-thirds of the total.Female students were mainly manifested unstable personality with extraversion,while male students were unstable introvents.In future we should take targeted psychological intervention measures.We are supposed to focus on each student's personality characteristic,temperament type and other specific conditions for a variety of education.
9.Expression of core-binding factor a1 by human skin fibroblasts induced in vitro.
Lianfu DENG ; Wei FENG ; Yue ZHANG ; Yaping ZHU
Chinese Journal of Surgery 2002;40(8):592-595
OBJECTIVETo investigate the probabilities of core-biding factor a1 (Cbfa1) expression by human skin fibroblasts induced in vitro.
METHODSThe fibroblasts were isolated, purified from human skin, and were grown in incubation in the media of TNF-alpha, BMP-2, and combined TNF-alpha and BMP-2 at certain concentrations, respectively. The changes in biological features of these fibroblasts correlated with osteogenesis were detected by immunohistochemistry and RT-PCR assay.
RESULTSTNF-alpha could switch phenotype of collagen in fibroblasts from Type I and III to Type I and induce fibroblasts to express Ras and BMP type I receptor (BMPR-IA). TNF-alpha in combination with BMP-2 could induce fibroblasts to express Cbfa1 and osteocalcin mRNA.
CONCLUSIONHuman skin fibroblast could be induced into pro-osteoblast expressing Cbfa1, an osteoblast-specific transcription factor and a regulation of osteoblast differentiation, and combined use of TNF-alpha and BMP-2 was one of the regulating factors.
Bone Morphogenetic Protein 2 ; Bone Morphogenetic Protein Receptors, Type I ; Bone Morphogenetic Proteins ; pharmacology ; Cells, Cultured ; Collagen ; biosynthesis ; Core Binding Factor Alpha 1 Subunit ; Core Binding Factors ; Fibroblasts ; metabolism ; Humans ; Neoplasm Proteins ; Osteocalcin ; biosynthesis ; Protein-Serine-Threonine Kinases ; biosynthesis ; RNA, Messenger ; analysis ; Receptors, Growth Factor ; biosynthesis ; Skin ; cytology ; Transcription Factors ; biosynthesis ; genetics ; Transforming Growth Factor beta ; Tumor Necrosis Factor-alpha ; pharmacology
10.Signaling of biglycan and cytokines in bovine intervertebral disc cells.
Journal of Central South University(Medical Sciences) 2011;36(9):889-894
OBJECTIVE:
To evaluate the effect of biglycan on the signaling of cytokines (epidermal growth factor, osteogenic protein-1, and interleukin-1) in bovine intervertebral disc cells.
METHODS:
Nucleoplasty (NP) and annulus fibrosus (AF) cells of the intervertebral disc tissues were isolated from the tails of young adult bovine. First, the cells were treated in 3 ways: Biglycan alone, cytokines alone (epidermal growth factor, osteogenic protein-1, or interleukin-1), and biglycan combined with cytonkines. Western blot was used to observe the singling of biglycan and cytokines in bovine intervertebral disc cells, and to identify the effect of biglycan on cytokines mentioned above.
RESULTS:
Biglycan upregulated the signaling (3- 4 folds) with the optimal effect at 10 min and 20 μmol/L both in the AF cells and NP cells. Epidermal growth factor, osteogenic protein-1, or interleukin-1 also upregulated the protein expression in the extracellular matrix of intervertebral disc cells. When combined different biglycan concentrations with epidermal growth factor, osteogenic protein-1, or interleukin-1 to treat the intervertebral disc cells, the concentration of biglycan rose, whereas the cytokine signal decreased both in the bovine AF and NP cells (P<0.01). There was no significant difference between the AF and NP cells.
CONCLUSION
Biglycan can adhere to the intervertebral disc cells to activate the extracellular signal-regulated kinase (ERK) pathway and this effect is time and concentration dependent. Byglycan can decrease not only the anabolism effect of epidermal growth factor and osteogenic protein-1, but also the catabolism effect of interleukin-1. This regulatory role of biglycan may be very important to maintain the metabolism balance. Biglycan may be good for the repair of intervertebral disc.
Animals
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Biglycan
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physiology
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Bone Morphogenetic Protein 7
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metabolism
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physiology
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Cattle
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Cells, Cultured
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Cytokines
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metabolism
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physiology
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Epidermal Growth Factor
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metabolism
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physiology
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Interleukin-1
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metabolism
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physiology
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Intervertebral Disc
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cytology
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metabolism
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Signal Transduction