Aim To investigate the effects of caffeic acid phenethyl ester (CAPE) on activation and proliferation of murine CD3+ T lymphocytes,and to study the mechanisms of its immunomodulative effects. Methods Lymphocytes suspension was isolated and prepared sterilely from murine lymph nodes, and was incubated with different concentrations of CAPE for 2 h, then polyclonal activator Con A was added to activate the lymphocytes; double-fluorescence staining and flow cytometry were used to detect CD69 and CD25 expressions to evaluate activation level of CD3+ T lymphocyte, and CFDA-SE staining plus flow cytometry were used to analyze CD3+ T lymphocyte proliferation. Results CAPE (0.5, 1, 5, 10 mg?L-1) could inhibit the expression of CD69 and CD25 of CD3+ T lymphocytes in a dose-dependent manner, and it also could inhibit T lymphocyte proliferation stimulated by Con A in a dose-dependent manner. Conclusion In vitro, CAPE could exert notably inhibition on activation and proliferation of murine CD3+ T lymphocytes,and its immunomodulative effects might result from affecting PLC-? signaling and MAPK-signaling pathways. So CAPE was a potentially effective immunoinhibitory agent.

AIM: To study the effects of [8-(diethylamino) octyl-3, 4, 5 -trimethoxybenzoate] (TMB-8), an intracellular Ca2+ antagonist, on the activation, proliferation and cell-cycle distribution of the mouse T lymphocytes stimulated by concanvalin A (Con A) in vitro. METHODS: After stimulated with Con A, T cells were treated with different concentrations of TMB-8 alone and its combination with cyclosporine A (CsA). The expression of CD69, the early marker of CD3+ T cell activation, was measured by FACS. The proliferation-related index was determined by carboxyl fluorescin diacetate succinmidyl ester (CFDA-SE) flow cytometry. The cell-cycle distribution was analyzed by propidium iodide staining.RESULTS: After 6 h culture, the activation rate of CD69+ T cell in Con A group was (74.88?1.88)%. 10, 20 and 40 ?mol/L of TMB-8 inhibited the expression of CD69 (P

This study was aimed to establish a HPLC method for the determination of isogarcinol. Daojin Inertsil WP300 C18 (4.6 mm× 150 mm, 5μm) was employed with methanol and water (75?25) as the mobile phase. The flow rate was 1.0 mL·min-1. The column temperature was set at 40°C. The detection wavelengthγ was set at 277 nm. The results showed that the linear range of isogarcinol was 0.005 7-0.039 9μg. The average recovery rate was 99.58%. The relative standard deviation (RSD) was 1.25%. The contents of isogarcinol inGarcinia mangostana,Garcinia oblongifolia,Garcinia oligantha,Cratoxylum cochinchinense andCalophyllum membranaceum were 0.285%, 0.199%, 0.857%, 0.161% and 0.006%, respectively. Isogarcinol was not detected inCratoxylum formosum orCalophyllum inophyllum. It was concluded that the method was convenient, accurate with high sensitivity, good stability and repeatability. It can be used for determination of isogarcinol content in Chinese herbal medicine.

Objective: To investigate the effect of occupational exposure on job burnout in nurses, and to analyze the mediating effect of negative emotion between occupational exposure and job burnout and the regulatory effect of supervisor support on occupational exposure and negative emotion. Methods: From September to December, 2015, simple random sampling was used to select 543 nurses from six tertiary hospitals in Zhejiang Province, China. The questionnaires consisted of occupational exposure risk questionnaire, negative emotion questionnaire, supervisor support questionnaire, and job burnout questionnaire. Results: The total score of occupational exposure risk in nurses was 11.43±7.19; the score of emotional exhaustion was 3.19±1.24, the score of low sense of personal accomplishment was 3.02±1.21, and the score of sense of working indifference was 2.24±1.06. There were significant differences in occupational exposure score between nurses with different sexes (t=2.61, P<0.01) and working years (F=4.49, P<0.01) . There were significant differences in the scores of emotional exhaustion and low sense of personal accomplishment in nurses with different sexes (t=5.25, P<0.001) and working years (t=-3.48, P<0.01) . Occupational exposure had positive effects on negative emotion (β=0.41, P<0.05) , emotional exhaustion (β=0.47, P<0.05) , sense of working indifference (β=0.42, P<0.05) , and low sense of personal accomplishment (β=0.17, P<0.05) . Negative emotion had a partial mediating effect between occupational exposure and emotional exhaustion (total effect size 30.5%, P<0.05) and between occupational exposure and sense of working indifference (total effect size 37.1%, P<0.05) . Negative emotion had a complete mediating effect between occupational exposure and low sense of personal accomplishment (β=0.08, P>0.05) . Supervisor support negatively regulate the effects of occupational exposure and negative emotion (F=21.73, P<0.001) . Conclusion In nurses, occupational exposure has a direct positive effect on job burnout and indirectly influences job burnout via negative emotion. Supervisor support can reduce the negative impact of occupational exposure on negative emotion.

Objective: To investigate the effect of leader-member exchange on nurses’sense of calling in workplace based on self-determination theory. Methods: A total of 381 nurses were randomly selected from five tertiary general hospitals in Zhejiang province, China from October to December, 2016. They were subjected to a survey using the Leader-Member Exchange Scale, Job Autonomy Scale, Core Self-Evaluation Scale, and Calling Scale. The mediating effect was used to test the procedures and the data were subjected to hierarchical regression analysis. Results: The leader-member exchange was positively correlated with job autonomy, core self-evaluation, and sense of calling (r=0.471, P<0.001; r=0.373, P<0.001; r=0.475, P<0.001) ; the leader-member exchange had a positive predictive effect on job autonomy and sense of calling (β= 0.47, P<0.001; β=0.48, P<0.001) ; the job autonomy had a partial mediating effect on the relationship between leader-member exchange and sense of calling (F=66.50, P<0.001) ; the core self-evaluation negatively adjusted the positive relationship between leader-member exchange and job autonomy (F=27.81, P<0.001) . Conclusion High-quality leader-member exchange enhances the sense of calling by improving staffs’ job autonomy and the core self-evaluation reduces the positive relationship between leader-member exchange and job autonomy.

Objective:To summarize the nursing experience in 2 patients with cardiogenic shock combined with acute respiratory distress syndrome(ARDS) treated by extracorporeal membrane oxygenation (ECMO) and transferred outer-hospital in long distance.Methods:There were many risk factors in this transport: high parameters of ECMO included V-A mode, 100% oxygen, and a flow of oxygen from 6 L/min to 10 L/min. High ventilator parameters included oxygen concentration of 100% and positive end-expiratory pressure of 15-17 cmH 2O(1 cmH 2O=0.098 kPa). Transport distance was up to 196 km. And the transport time was up to 2 h 36 min. In this regard, we carried out adequate preparations before transport and professional cares during transport. The main points were as follows: setted up a professional transport team; prepared adequate power and oxygen supply; reduced the number of interruption of ECMO and ventilator support during transport; provided the reasonable remedial actions when ECMO and ventilator support were interrupted. Results:Two patients arrived at the destination safely.Conclusions:Adequate preparations before transport and professional cares during transport could effectively avoid and respond to the occurrence of adverse events, and it is feasible and safe to patients supported by ECMO for long distance outer-hospital transport.

Objective:To investigate the current situation of knowledge, attitude and practice level of ICU nurses in the prevention of medical device-related pressure injuries (MDRPI) in critically ill patients and analyze its influencing factors.Methods:From May to August 2019, 540 ICU nurses in 8 Class Ⅲ general hospitals in Zhejiang province were surveyed by using "MDRPI Prevention Knowledge, Attitude and Practice Questionnaire for ICU Nurses". Current situation and influencing factors of knowledge, attitude and practice of ICU nurses in the prevention of MDRPI were analyzed and relationships among prevention knowledge, attitude and practice were explored.Results:The overall average score of knowledge, attitude and practice level of ICU nurses in the prevention of MDRPI was (3.87±0.46) . Of which, the average score of knowledge dimension items was (3.43±0.69) , the average score of attitude dimension items was (4.13±0.51) , and the average score of practice dimension items was (4.06±0.56) . There were statistically significant differences in the overall average scores of knowledge, attitude and practice in the prevention of MDRPI in ICU nurses with different genders, marital status, years of work, titles, duties, whether they are members of the wound stoma or pressure ulcer group, whether they have cared for MDRPI patients, whether they have received MDRPI training ( P<0.05) . Multiple linear regression analysis showed that years of work, whether they had cared for MDRPI patients and whether they had received training in MDRPI were influencing factors of knowledge, attitude and practice level of ICU nurses in the prevention of MDRPI ( P<0.05) . ICU nurses MDRPI prevention knowledge, attitude and practice were positively correlated ( P<0.05) . Conclusions:ICU nurses have a more positive attitude and behavior towards MDRPI prevention, but their knowledge of MDRPI prevention is relatively lacking. Nursing managers should attach importance to training on ICU nurses' knowledge of MDRPI prevention so as to improve their MDRPI recognition and nursing ability.

Objective:To discuss the relationship between supracapsular secretion drainage of tracheal intubation before percutaneous dilational tracheotomy (PDT) and the incidence of early-onset VAP (within 5 days) after PDT.Methods:By the convenient sampling method, a total of 160 patients who underwent PDT in ICU of Shaoxing People's Hospital from March 2013 to March 2019 were selected as the research objects and they were divided into group A and group B according to the random number table method, 80 cases in each group. In group A, the research team intervened to drain supracapsular secretion before PDT and bedside nurses cooperated with the doctor to complete other operations on the PDT. In group B, the PDT operation was entirely completed by the bedside nurses and the research team did not intervene. The drainage volume of supracapsular secretion in group A was recorded and the proportion of patients who underwent supracapsular secretion drainage in group B was calculated. The incidence of VAP, ICU mechanical ventilation time, ICU hospitalization time and ICU hospitalization cost within 5 d after PDT were compared between the two groups.Results:Before PDT, patients in group A drained (3.88±2.34) ml of supracapsular secretion. The proportion of bedside nurses in group B who drained secretions before PDT was 53.75% (43/80) . The incidence of VAP within 5 d after PDT in group A was 6.25% (5/80) , which was lower than 17.50% (14/80) in group B, and the difference was statistically significant ( P<0.05) . The ICU mechanical ventilation time and hospital stay of patients in group A were respectively (28.75±8.59) d and (30.91±9.12) d, which were lower than (32.91±8.53) d and (34.28±9.54) d in group B, and the differences were statistically significant ( P<0.05) . The ICU hospitalization cost of group A was (14.98±4.54) ten thousand yuan, which was lower than (16.93±5.37) ten thousand yuan in group B, and the difference was statistically significant ( P<0.05) . Conclusions:Supracapsular secretion drainage of tracheal intubation before PDT can reduce intraoperative aspiration of supracapsular secretion, reduce the incidence of early-onset VAP after PDT and improve prognosis of patients.

With the rapid development of computing methods and computing power, the ability of artificial intelligence in problem modelling and analysis has been improved rapidly. Artificial intelligence has been gradually introduced into the field of medical care and nursing, and played an important role in assisting medical care and nursing. This paper mainly discusses the progress of artificial intelligence technology in nursing, and analyzes the existing problems and challenges, in order to provide new ideas for artificial intelligence in supporting the development of nursing more effectively.

Objective To research the effect of the identity recognition system based on the Itouch in the patients care .Methods The study group used the identification system of Itouch in all aspects of nursing intervention .Patients without this system were selected as the control group .The rate of operation error , time spent on writing and patient satisfaction rate were compared between groups .Results The error rates of intravenous infusion , oral medicine distribution , specimen collection intravenous injection and skin test were 0.58%,1.42%,0.45%,0.55%,0.62%in the study group, and 0.72%,2.23%,0.79%,0.64%,2.93%in the control group, there were significant differences between two groups (χ2 =5.21,38.37,6.39,7.26,4.85, respectively;P<0.05).The number of patients who were very satisfied , satisfied and dissatisfied was 685,506 and 55 in the study group and 566 , 566 and 146 in the control group , there were significant differences between two groups (χ2 =55.48,P<0.01).The time nurses spent on writing , signature, changing orders and checking were (24 ±12)min,(16 ±3)min,(14 ±5)min in the study group, and (206 ±42)min,(72 ±6)min,(69 ± 17)min in the control group.There were significant differences between two groups (t=22.82,45.72,17.00, respectively;P <0.01).Conclusions The identity recognition system based on the Itouch was helpful to improve the accuracy rate , work efficiency and the satisfaction rate of patients and to reduce the nursing adverse events.