Objective To establish the standard for the quality control of Shanlamei Granule.Method Chimonanthus nitens Oliver.was identified quantitativ ely by thin -layer chromatography(TLC),quercetin and kaempferol in Shanlamei Granule were determined by RP -HPLC.Results The TLC Spots were highly clear without the interference of negative control and were reproductive.The c alibration curve for quercetin was l inear(r =0.9999)in the range of 1.61～25.72?g /mL and the mean recovery was 98.89%,RSD being 1.21%(n=5);for Kaempferol ,the calibration cu rve was linear(r =0.9999)in the range of 2.16～34.60?g /mL and the mean recovery was 97.75%,RSD being 1.53%(n=5).Conclusion This method is accurate,simple,sensitive and reliable,and it can be used for the quality control of Shanlamei Granule.

Objective To study the pathogenesis of acute intravascular hemolysis following copper needle electrothrombosis for treating a huge cavernous hemangioma. Methods (1) The full course of severe intravascular hemolysis in a patient with huge cavemous hemangioma treated by copper needle electrontrombosis was observed. (2)In vitro experiment, a copper needle, an iron needle and a stainlss-steel needle were put in citrated anticoagulant healthy blood specimens respectively. The needles were electrified with 3～6 voltages DC for 30 minutes. Then the free hemoglobin of the specimens was tested before,during and after electrfication. The red cell morphology was observed under a light microscope. Results Severe intravascular hemolysis occurred in twice treatments with copper needle electrothrombosis. In vitro experiment showed that erythroclasis occurred after needle electrifying with obvious increasing of free plasma hemoglobin of the specimens. The higher the voltage and the longer the electrification of the needle, the more severe the hemocytocatheresis. Conclusions Severe intravascular hemolysis may happen during copper needle electrothrombosis for treating huge cavernous hemangioma. Red blood cell injury directly caused by electrification is the main factor of intravascular hemolysis.

Objective To study the pathogenesis of acute intravascular hemolysis following copper needle electrothrombosis for treating a huge cavernous hemangioma. Methods (1) The full course of severe intravascular hemolysis in a patient with huge cavemous hemangioma treated by copper needle electrontrombosis was observed. (2)In vitro experiment, a copper needle, an iron needle and a stainlss-steel needle were put in citrated anticoagulant healthy blood specimens respectively. The needles were electrified with 3～6 voltages DC for 30 minutes. Then the free hemoglobin of the specimens was tested before,during and after electrfication. The red cell morphology was observed under a light microscope. Results Severe intravascular hemolysis occurred in twice treatments with copper needle electrothrombosis. In vitro experiment showed that erythroclasis occurred after needle electrifying with obvious increasing of free plasma hemoglobin of the specimens. The higher the voltage and the longer the electrification of the needle, the more severe the hemocytocatheresis. Conclusions Severe intravascular hemolysis may happen during copper needle electrothrombosis for treating huge cavernous hemangioma. Red blood cell injury directly caused by electrification is the main factor of intravascular hemolysis.

Spine fusion stabilize adjacent vertebral segments by achieving bone union. The preferred method of spine fusion involves decortication of the host bed and transplantation of autologous bone graft from the iliac crest. Although autologous bone grafting is the gold standard, this procedure has significant complication. Gene therapy represents the new frontier of medical science that holds much promise for the improvement of spinal arthrodesis. Studies have shown the efficacy in using liposome-mediated and adenovirus-mediated gene transfer of bone morphogenetic protein (BMP) and related gene in animal models. Several studies in which gene transfer has been used specifically to enchance spine fusion in animal models are reviewed. Current main areas of research, including the elucidation of gene expression profiles during bone formation and the development of new gene transfer vehicles, promises the development of clinically applicable techniques in the near future.
Animals ; Bone Morphogenetic Proteins ; genetics ; physiology ; Genetic Therapy ; methods ; Genetic Vectors ; Osteogenesis ; genetics ; Spinal Fusion ; methods ; Transfection

ObjectiveTo explore the effect of estrogen on osteoblast apoptosis induced by serum hungry in vitro.MethodsOsteoblasts of second or third generation from newly born SD rats calvaria were divided randomly into the control group, serum hungry group and serum hungry with estrogen group. Cells of each group were incubated for 24 h, 48 h, 72 h, 5 d, 7 d and 14 d, then labeled using TUNEL staining and examined for morphological characteristics of apoptotic cell under light microscopy after incubated for 72 h. The rates of apoptotic cells of each group were examined with flow cytometry.ResultsThe cells of the control group showed normal appears, the serum hungry group had many cells with purple and blue particles in nuclei, but serum hungry with estrogen group had less such cells. The rate of apoptotic cell significantly increased in serum hungry group and decreased in serum hungry with estrogen group compared with the control group examined with flow cytometry (P<0.05).ConclusionEstrogen can repress osteoblasts apoptosis of rats induced by serum hungry.

ObjectiveTo explore the mechanism of estrogen inhibiting osteoblast apoptosis induced with serum hungry.MethodsOsteoblasts of the second or third generation from newly born SD rats calvaria were divided randomly into three groups: control group,serum hungry group,serum hungry with estrogen group.Cells of each group were incubated for 1,2,3,5,7 or 14 d,and then were stained immunohistochemically.The rates of positive cells of each group were analyzed.ResultsThere was a little positive expression of Bax,Bcl2 and Fas in control group.The expression of Bax and Fas were significantly increased(P<0.05)in serum hungry group,peak time was 14 d,but the expression of Bcl-2 were not affected.Compared with that of serum hungry group,the expression of Bax and Fas significantly decreased(P<0.05) in serum hungry and estrogen group,peak time was still 14 d,while that of Bcl-2 increased(P<0.05).ConclusionSerum hungry can increase the expression of Bax and Fas in osteoblast,that can be inhibited by estrogen.Estrogen can also increase the expression of Bcl-2 in osteoblast.All of these may play a role in inhibiting osteoblast apoptosis induced with serum hungry.

To evaluate the application of blood salvage techmque in spine orthopaedic operation. 26 cases of spine orthopaedic operations were divided into two groups. Group A received homologous blood transfusion. Group B received intraoperative blood salvage by cell saver in spine orthopaedic operations. No complications of transfusion and dysfunciton were found in all pateints. The results showed that blood salvage technique can decrease effectively the need of homologous blood transfusion in spine orthopaedic operation and can be used safely in clinical practice.
Adolescent ; Adult ; Blood Transfusion, Autologous ; methods ; Child ; Child, Preschool ; Female ; Humans ; Male ; Orthopedic Procedures ; Scoliosis ; surgery

Objectives To investigate the epidemiological and clinical characteristics of dyslipidemia as well as its treatment and influence on accompanying diseases in impaired glucose status among inpatients. Methods A cross-sectional survey was conducted among the inpatients registered in ten university hospitals of Guangdong, China during the week before the Diabetes Day in 2004. The fasting blood glucose (FBG), lipid profiles, BMI, waist to hip ratio (WHR) and concomitant disorders of the first screen during the hospitalization period were recorded. Those who had FBG level from 5.6 to 6. 9 mmol/L and not been previously diagnosed diabetes (PDM) underwent oral glucose tolerance test (OGTF). Results Of the 8753 inpatients investigated, 1067 eases had complete medical records(CMR case) including PDM cases and previously non-diagnosed diabetes ones with FBG ≥ 5. 6 mmol/L. Of the previously non-diagnosed diabetes cases with FBG levels from 5.6 to 6.9 mmmol/L, 65.8% accepted OGTT. Of the CMR cases, 41.9% had PDM, 21.7% was newly diagnosed diabetes mellitus (NDM), 29. 1% had impaired glucose regulation (IGR) and only 7.3% had normal glucose tolerance (NGT). The TG levels in NDM and PDM group were higher than those in IGR and NGT group (P < 0.05, respectively). The HDL-C levels in IGR, NDM and PDM group were lower than those in NGT group (P < 0.05, respectively). Sixty-nine point six percent of the diabetes mellitus (DM) inpatients was accompanied with dyslipidemia and the rate was higher than those in NGT (56.4%) and IGR inpatients (52.5%, P <0.05, respectively). Only 22. 8% of the PDM inpatients underwent treatment of dyslipidaemia and just 3.4% achieved the target suggested by the guideline of ATP-Ⅲ. BMI was higher and waistline longer in the PDM and NDM inpatients than those in the NGT cases (P <0.05, respectively). Seventy-two point eight percent of the PDM inpatients was complicated with more than one type of vascular diseases. Nine point seven percent and 0. 2% of the NDM inpatients were tormented by diabetic nephropathy and diabetic retinopathy respectively. Conclusions More inpatients with accompany DM or IGR had concomitant dyslipidemia than those with NGT, which included hypertriglyccridemia, hypo-high-density lipoproteinemia and metabolic syndrome. Concomitant vascular diseases were more frequently found in PDM inpatients than in the others. Some of the NDM and IGT inpatients were complicated with microvascular diseases.