The cDNA of Insulin-like growth factor binding protein 3 was subcloned into a eukaryotic secretory expression vector pSectagA to construct pSectag-IGFBP3. Human renal cell carcinoma (RCC) 786-0 cells were transfected with pSectag-IGFBP3 using lipofectamine 2000. After 48 h, the secretory IGFBP-3 was tested and identified by western blotting. Meanwhile, Annexin V-EGFP stain was used to analyze the apoptosis of 786-0 cells induced by IGFBP-3. Secretory IGFBP-3 protein could express successfully in the 786-0 cells and the expressed IGFBP-3 directly displayed an apoptotic effect on the host cells. This work provides a basis for further study on the apoptosis-inducing mechanism of IGFBP-3 and the development of a new anti-tumor drug.
Apoptosis ; drug effects ; Base Sequence ; Carcinoma, Renal Cell ; metabolism ; pathology ; Humans ; Insulin-Like Growth Factor Binding Protein 3 ; genetics ; secretion ; Kidney Neoplasms ; metabolism ; pathology ; Molecular Sequence Data ; Recombinant Fusion Proteins ; biosynthesis ; genetics ; physiology ; Transfection ; Tumor Cells, Cultured

Objective To investigate the effect on blood coagulation function and immune function in patients with gas-tric cancer by laparoscopic radical gastrectomy and open operation. Methods In our hospital from June 2011 to June 2014,selected hospitalized patients with gastric cancer 30 cases as the research object,15 cases were undergone tradi-tional open operation in patients with the establishment of the control group, the remaining 15 cases received laparo-scopic radical resection of gastric cancer,set up the observation group,the changes of the TT,APTT,INR,FIB,D-D of blood coagulation indexes of two groups were compared,and changes in two groups of patients with immune function indexes of CD3+,CD4+,CD8+,CD4+/CD8+,and the operation time,bleeding amount of two groups of operation,ambula-tion time, hospital stay, postoperative complications were compared between two groups. Results In the observation group and the control group after 24 h,the FIB,D-D was significantly higher than the preoperative,and the observa-tion group patients after operation significantly were higher than the control group (P<0.05). After operation 1 weeks,CD3+,CD4+, CD8+, CD4+/CD8+of observation group showed no significant changes (P>0.05). CD3+, CD4+, CD4+/CD8+of control group after 1 weeks significantly changed compared with the preoperative, and CD3+, CD4+, CD4+/CD8+ of observation group compared with the control group,the difference was statistically significant(P<0.05). But CD8+of the control group had no obvious change before and after surgery(P>0.05). The observation group patients' out of bed activity time, postoperative hospitalization time was significantly shorter than that in the control group,the amount of bleeding observation group was significantly less than that the control group(P<0.05). Conclusion Laparoscopic radical gastrectomy for gastric cancer curative has good effect, compared with open radical gastrectomy for gastric cancer with less bleed-ing, fewer complications, patients recovered quickly, the advantages of immune function in patients with small inter-ference, but the effects of laparoscopic operation on blood coagulation in patients with more significant, corresponding measures should be taken to prevent the formation of thrombus coagulation abnormalities caused by postoperative.