Objective To analyze serum markers of transfusion,transmitted infections in pre,transfusion patients and investigate the cause of clinical transfusion related infections.Methods HBsAg,Syphilis antibody,Anti-HCV,and Anti-HIV were detected by ELISA in 3380 Cases.Results Among 3380 patients detected,HBV positive,TRUST positive,HCV positive and HIV positive were 378cases(11.3%),3 cases(0.09%),65 cases(1.9%)and 4 cases(0.12%)respectively.Conclusion Through the serology detections in patients before transfusion,We can identify the patient's health state,avoid the medical dispute resulted from the iatrogenic dissemination diseases and eventually protect the benefits between patients and medical personnnels.

砄bjective: To study the effects of functinal appliances combined with activator headgear in the treatment of Class II division 1 malocclusion with or without high mandibular angle. Methods: 5 cases (10～12 years old) of class Ⅱ division 1 malocclusion with high mandibular angle (FMA≥34?,HMA) and 8 cases (9～12 years old) without the high angle (FMA

0.05). IH scores of 15-PGDH in chorion of placenta and fetal membranes in PL were 1.5?0.6,2.3?0.8, respectively, in TL were 2.6?0.8,3.0?0.7, respectively, and in control group were 4.4?1.1, 4.1?1.2,respectively. IH scores in PL and TL were obviously lower than those in control group(P

Molecular-targeted therapy is a new method and tendency in the treatment of hepatocellular carcinoma (HCC). To date, sorafinib, a multi-targeted gent, is the only one proved to be effective in improving the survival of patients with advanced HCC. Sorafinib is also the first line systemic agent for advanced HCC. Other multi-targeted agents, such as sunitinib, are also proved to be effective. Erlotinib, gefitinib and eetuximab, which target epidermal growth factor receptor, show effectiveness but still need further investigation. Bevacizumab, which targets vascular endothelial growth factor and vascular endothelial growth factor receptor, shows excellent results and deserves more clinical trials. The effects of bortezomib, sirolimus and imatinib, which target other pathways, are still under investigation. The future studies of molecular-targeted therapy for HCC should be focused on the combination of different targeted medicine, and combination of molecular-targeted therapy and chemotherapy, as well as individualized therapy.

Objective:To investigate the variation of nm23-H1 gene structure and its expression in oral squamous cell carcinoma (OSCC) and to study the relationship between nm23-H1 expression and OSCC metastasis to neck lymph nodes.Methods:nm23-H1 gene in the samples of 30 cases of OSCC,7 of normal oral mucous tissue,human OSCC cell line TSCCa and human oral metastatic cancer cell line GNM was extracted and amplified by PCR. PCR products were investegated with SSCP analysis;nm23 m RNA level in the samples was studied by in situ hybridization.The relationship between mRNA expression and metastasis to neck lymph nodes was analyzed statistically.Results： All samples showed no change in nm23-H1 gene structure except deletion in one case of OSCC;7 out of 9 cases with metastasis to neck lymph nodes showed no hybridization signal of nm23-H1 mRNA,however,only 5 were found no nm23-H1 mRNA in 21 cases without metastasis,positive rate of nm23-H1 expression in GNM cells was lower than that in TSCCa (41.2% versus 63.1%);The expression of nm23-H1 gene showed significant negative relationship with neck lymph nodes metastasis (P<0.01);no relationship was found between expression of nm23-H1 gene and cancer pathological classification.Conclusion:The change of nm23-H1 expression doesn't result from the variation of gene structure;the product of nm23-H1 gene probably inhibit OSCC metastasis to neck lymph nodes.

objective: To evaluate the clinical effect of various materials used in the reconstruction of mandibular defects retrospectively. Methods: 467 patients with mandibular defects were treated with various implantation materials during last 30 years and 395 of them were followed up.Results: 217 were satisfied with their mouth opening, occlusion and maxillofacial contour after sugery, 189 of them had bone transplanation and 28 had non biomateria transplantaion; 91 were partly satisfied, 72 of them had bone trasplantation and 19 had non biomateria; 87 were not satisfied, 11 of them had bone trasplantation and 76 had non biomaterial. Conclusion: Bone especially auto bone transplantation is more effective than non biomaterial in the reconstruction of mandibular deffects.

Objetive:To analyze the fixation methods and sites in treatment of zygomatic complex fractures of 152 patients. Methods:The clinical data of 152 patients from 1990 to 2004 were investigated and analyzed, which focused on the changing trend of etiology and treatment methods. Results:Traffic accident was the primary reason for zygomatic complex fractures in China.152 patients received primary, timely reduction and fixation; Scalp coronal incision in combination with other incisions or alone was applied in 108 patients, the rest were treated by single local incisions. Rigid internal fixation was applied in 123 cases, and the single application of transosseous wire fixation was performed in 22 patients. 328 Ti plates were used. As to the fixation sites, 96 were applied at the zygomatic-frontal suture, 45 plates at zygomatic-maxillary suture and zygomatic crest, 92 plates at zygomatic-temporal suture, and 95 plates across the fracture line. Conclusion:Scalp coronal incision in combination with other incisions or alone can provide satisfactory outcome. Early, timely reduction and rigid internal fixation may result in better treatment results. The sites are focused at zygomatic-frontal suture, zygomatic-maxillary suture and zygomatic crest, zygomatic-temporal suture and the fracture line which restore the horizontal and vertical force route.

砄bjective:To investigate the variation of nm23 H1 gene structure and its expression in oral squamous cell carcinoma (OSCC) and to study the relationship between nm23 H1 expression and OSCC metastasis to neck lymph nodes.Methods: nm23 H1 gene in the samples of 30 cases of OSCC,7 of normal oral mucous tissue,human OSCC cell line TSCCa and human oral metastatic cancer cell line GNM was extracted and amplified by PCR. PCR products were investegated with SSCP analysis; nm23 m RNA level in the samples was studied by in situ hybridization.The relationship between mRNA expression and metastasis to neck lymph nodes was analyzed statistically.Results: All samples showed no change in nm23 H1 gene structure except deletion in one case of OSCC;7 out of 9 cases with metastasis to neck lymph nodes showed no hybridization signal of nm23 H1 mRNA,however,only 5 were found no nm23 H1 mRNA in 21 cases without metastasis,positive rate of nm23 H1 expression in GNM cells was lower than that in TSCCa (41.2% versus 63.1%);The expression of nm23 H1 gene showed significant negative relationship with neck lymph nodes metastasis ( P

AIM: To study the role of adrenomedullin(AM) in the pathogenesis of hypoxic pulmonary hypertension. METHODS: Rats were exposed to chronic hypoxia for 14 days. After the measurement of the right ventricular systolic pressure (RVSP), the rats were executed. The weight of the right ventricle (RV), the left ventricle(LV) and the ventricular septum(SP) were determined. The ration RV/(LV+SP) was used to express the thickness of RV. In situ hybridization was used for the detection of the expression of AM mRNA in the lung and RV. RESULTS: The RVSP in the hypoxic group was (63.63?3.42) mmHg,which was significantly higher than that in control group [(34.13?3.40) mmHg]. The RV/(LV+SP) in hypoxic group was 0.439?0.039,which was increased obviously when compared with that of control (0.230?0.025). The level of AM mRNA expressed in the RV in the hypoxia group was significantly higher than that in the control group. CONCLUSION: The expression of AM mRNA in RV increased in the hypoxic condition, which suggests that AM may attenuate the inappropriate increase in pulmonary artery pressure.

Objective: To study the characteristics of CT imaging of adult ischemic type moyamoya disease, involving CT plain scan, CT perfusion imaging (CTP) and CT angiography (CTA). Methods:A retrospective analysis was made of the imaging data of 20 adult ischemic type moyamoya patients, including CT plain scan, CT perfusion imaging and CT angiography. CT vascular imaging features were graded I-VI with reference to Suzuki vascular grading. Results:Among the 20 patients with adult ischemic type moyamoya , ①CT plain scan: Old infarct lesions occurred in 13 cases(65%), 1 case suffered from acute cerebral infarction(5%), and negative patients totalled 6 (30%);②CT perfusion:5 cases(25%) were normal perfusion, 15 cases(75%) showed obviously low perfusion and local high perfusion;③CT angiography:patients of grade I to VI were respectively 1, 2, 5, 6, 4 and 2. Conclusion:Cerebral infarction lesions associated with adult ischemic type moyamoya disease are distributed mainly in the frontal and parietal cortex, or in watershed regions. Cerebral perfusion is characterized by normal or uneven blood perfusion, especially low perfusion. Vascular imaging manifests mostly degree III and IV, which belong to the middle phase of moyamoya disease.