OBJECTIVE To investigate the knowledge about hand hygiene among the healthcare workers in the infectious disease hospital and improve their hand hygiene compliance.METHODS A standardized questionnaire was sent to 364 healthcare workers in the infectious disease hospital.The following data investigated anonymously were collected.RESULTS All the healthcare workers' answers to the choice questions were right(100.0%).However,there were significant differences in the answers to the memorized questions among the healthcare workers with different careers.The correct answers among the nurses were the highest(97.7% and 94.8%,respectively);among the doctors were 71.4% and 34.7%;and among the medical technicians were 21.5%,and 8.6%,respectively.But there were no significant differences between sexes,education degrees and service length in the three groups.CONCLUSIONS The healthcare workers of our hospital are well in the field of basic knowledge about hand hygiene.However,there are differences in the answers to the memorized questions among healthcare workers with different careers.It need to enhance the systems of training and examination for workers,especially for the doctors,technicians and all the new staff.Alcohol-based hand rub(AHR),which is prone to practice,would replace hand-washing.And it should be more supervised.

BACKGROUND:Colagen sponges are applied for hemostatic use, wound healing, and residual cavity filing, which have great values in clinical application and scientific research.
OBJECTIVE:To investigate the biological properties, biocompatibility and biodegradability of colagen spongesin vivo.
METHODS: The spatial structure, pore diameter and porosity of colagen sponges were characterized by scanning electron microscopy. Transmission electron microscopy was used to observe the conformation of colagen sponges. The secondary structure and thermal denaturation temperature of colagen sponges were
analyzed by circular dichroism spectrum. Colagen sponges were implanted intramuscularly into the spinal cord of New Zealand rabbits to observe the degradation and absorption and histological changesin vivo.
RESULTS AND CONCLUSION: Colagen sponges had porous structure with varying pore sizes ranging
40-150 μm, the mean pore size of 100 μm, the thickness wal of 1 μm, and a porosity of approximately 95.8%. Colagen sponges had a typical porous structure and periodic light and dark zones. The solution of colagen
sponges had a weak positive band near 220 nm and an intense negative band near 206 nm, which indicated a classic triple helix. And the secondary structure and thermal stability of colagen sponges were similar to that of
liquid colagen. Colagen sponges began to degrade at 4 weeks, and remained 20% at 12 weeks. These sponges had been associated with foreign body response and inflammation within 2 weeks after implantation. With wound healing, inflammatory reactions gradualy reduced and disappeared. During the implantation and degradation of sponges, no significant fibrous capsule formed and no tissue necrosis occurred at implantation site, indicating that colagen sponges have good performance in bioactivity, biocompatibility and degradation.