Objective To investigate the X-ray photographic and magnetic resonance imaging (MRI) features of hip pigmented villonodular synovitis (PVNS). Methods A retrospective analysis was carried out in 19 patients with hip PVNS confirmed by operation and pathological examination.The X-ray plain photography in 10 patients and MRI features in 19 patients (of which 9 received enhanced scan) were analyzed.Results (1)The results of X-ray photography were as follows:Of 10 patients,6 showed swollen joint capsule,2 had higher-density masses around the joint;joint space narrowing was found in all of the 10 patients;except 2 patients,8 showed various degrees of bone erosion in the margin area of the joint,in the intertrochanteric area and around neck ,and showed capsule-like clear focus area with sclerotic margin in the joint surface of the bone;bonespur was found in the edge of the joints of 4 patients,of which 3 were complicated with osteoporosis.(2)The manifestations of MRI features:of 19 patients with hip PVNS,17 were differentiated as the diffused and 2 as the focalized.All of 17 diffused hip PVNS showed different degrees of diffuse hyperplasia of synovium,5 of them had intra-articular effusion,13 showed multiple-diffused nodular synovial changes with low signals of T1WI and T2WI,14 showed different degrees of bony erosion and depression in acetabulum,in the head of the femur and around the neck,which signals were similar to those with synovial hyperplasia.Of the 14 patients,8 foci presented circular low signals,capsule-like clear images under the bony joint surface with medium to low signals of T1WI and T2WI,and diffused swelling close to medullar cavities with sheet-like high signals of T2WI/STIR.The results of contrast-enhanced scanning for 8 of the 14 patients showed enhanced signals in the joint synovium and focus area of joint bones.Two focalized PVNS patients showed single mass on the outer flank of the femoral neck with medium to low T1WI signal and with medium to high T2WI signal,and also presented multiple dot-like and nodule-like foci with low T1WI and T2WI signals.One of the 2 focalized PVNS patients showed arc-shaped bone erosion at the outer margin of the femur.Conclusion MRI features of hip pigmented villonodular synovitis exert specific characteristics,which are valuable for the diagosis of PVNS;X-ray plain photography has indicative value for the diagnosis,but it has limitation.

Objective To observe the change of tumor cells on sodium phenylacetate(NaPA) treating G422 glioma mice,and explore its mechanism.Methods Two kinds of mice models(intracranial and muscular G422 glioblasloma cells) were established,and were divided into five groups,among which 3 groups for expe rimental groups,they were received NaPA(1200,800,400 mg/kg?d).In the other two groups,the positive control group was administered BCNU(20 mg/kg) only one time.The negative control group was given saline 24 h later tumor inoculation,these mice bagan to be administered.The experimental group and negative control group were injected NaPA and saline for 14 days respectively.After that,the drug toxicity,the survival period,survival rate of the mice were observed,and the pathology and ultrastructure of glioma in every group were also observed.The muscular tumor mice were sacrificed for measurement of tumor suppression rate.Results NaPA can prolong the life span of the mice with glioma;it has concentration dependent inhibition to glioma proliferation;the pathology and electron microscopy showed the decrease of glioma nuclei fission image treated by NaPA,the increase of rough endoplasmic reliculum and the cell proptosis were found,it demonstrated the tumor cells had differentiation trend.Conclusion NaPA had antitumor effect by inducing glioma cell differentiation and inhibiting the growth of tumor.

Objective To observe the curative effect of sodium phenylacetate (NaPA) on induced differentiation in human glioma cells P 168 in vitro,and preliminarily probe into its mechanism.Methods MTT assay flow cytometry and cell microscope were used to test the changes of P 168 cells treated by different concentrations of NaPA in vitro, and observed ultrastructureal changes of tumor cells.Results NaPA could inhibite the growth of human glioma,show the inhibition effect of time concentration dependent,the flow cytometric was used to find S phase is 11.99% or 13.17% in the NaPA treated P 168 cells and 20.17% in the NaPA untreated P 168 cells. Electron microscope of NaPA treated P 168 cells demonstrated there were rich mitochondria, rough endoplasmic reticulum and elastic filament,but in NaPA untreated P 168 cells,there were numerous scattered polyribosomes appeared.Conclusion NaPA not only inhibit the growth of P 168 haman glioma cells, but also remarkably induce the differentiation of these cells in vitro.

Objectives To investigate the clinical and pathological characters , treatment of patients with Angioimmunoblastic T cell lymphoma(AITL). Methods From 1997 to 2004, 14 patients with AITL were reviewed retrospectively in our hospital. Results The most common symptoms at the presentation included general lymphadenopathy. 9 patients had fever. 3 patients had autoimmune hemolytic anemia.The histopathologic characteristics of AITL was generalized as:the damage of normal structure of lymphonodus,the proliferation of immunoblastic cell and arborescent supervascularization. All immunophenotyping were T cell type.14 patients were with ProMACE-CytaBOM regimen.The overall response rate was 57% and CR 3 cases, PR 5 cases. Median survival times was 25 months. 2-year survival was 60 %. Conclusions The most cases with AITL runs an aggressive course.The disease may progress rapidly and have unfavorable prognosis. Therefore further studies are repuired to improve the outcome.

Objective To establish a method for reducing the dose to the eye lens of interventional staff,and provide the data basis for improving radiological protection measures.Methods One piece of interventional equipment coupled with conventional auxiliary protective devices and two types of common neural interventional procedures were selected to monitor 46 and 35 procedures before and after the device modification.The doses to the eye lens of staff were measured with direct-reading dosimeters for analysis of dose trends.Results After modification of the devices,the average dose to the left eye lens decreased from (9.71 ±10.86) to (3.23 ±5.59) μSv for the first operator,from (9.51 ± 12.34) to (0.68 ± 0.78) μSv for the second in cerebral angiography;whereas the dose decreased from (14.83 ± 19.13) to (4.17±4.59) for the first operator and from (14.12±21.76) to (1.23 ±1.57)μSv for the second in embolization procedure,respectively.The left eye lens doses measured before and after the modification showed significant difference (U =-2.760,-2.467,-1.967,-2.655,P ＜0.05).Conclusions The modification of the auxiliary radiological protective devices may effectively reduce the dose to the eye lens dose.This method was shown to be feasible for the improvement of radiological protection of interventional staff.

Objective To develop a PCR-mverse dot blot hybridization(RDB)assay to rapidly detect pathogenic mycoplasmas in genitourinary tract.Methods Universal primers were designed and applied to amplify the 16S rRNA gone of ureaplasma parvum(Up),ureaplasma urealyticum(Uu),Mycoplasma genitalium(Mg),Mycoplasma hominis(Mh)by using nestcd PCR.Specific nucleotide probes of Up,Uu,Mg and Mh Were constructed and immobilized onto nylon membranes.PCR products were denatured and hybridized、with specific oligonucleofide probes on nylon membrane.The sensitivity and specificity of the PCR-RDB assay were evaluated based.on the hybddizafion results.Also,PCR-RDB Was utilized to detect pathogenic mycoplasmas from 60 clinical samples.Results The four probes selectively hybridized with the PCR product of corresponding mycoplasmas,and no cross hybridization was observed.The detection limit of PCR-RDB Was one colony forming unit(CFU)of mycoplasma.Out of the 60 clinical samples、19were positive for mycoplasm,Mixed infections were found in three samples,including two coinfected with Up and Uu and one with Uu and Mg.Conclusion PCR-RDB is a rapid,specific and sensitive approach to the identification of pathogenic mycoplasmas in urogenital tract.

Objective To assess the vitro susceptibility to 6 antimicrobial agents and genotypes of clinical isolates of Chlamydia trachomatis (Ct) from Guangzhou region. Methods Ct was isolated from clinical specimens by using McCoy cell culture and subjected to propagation. The minimum inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) of 6 antimicrobial agents (clarithromycin, roxithromycin, azithromycin, doxycycline, tetracycline, ofloxacin) against Ct isolates were determined in McCoy cell culture. Nested PCR was performed to amplify the outer membrane protein 1 (omp1) VS1-2 gene followed by sequencing. Results Seventy-six Ct strains were isolated from 346 urogenital specimens, and 40 strains met the require ments for susceptibility testing after serial propagation. The MIC50/MIC90 of clarithromycin, azithromycin, roxi thromycin, doxycycline, tetracycline and ofloxacin were as follows: 0.008/0.032, 0.080/0.160, 0.125/0.500, 0.032/0.064, 0.250/0.500 and 0.500/1.000 mg/L. Seven genotypes were observed. The most prevalent geno types in decreasing order were E (14, 35%), J (10, 25%)and F (6, 15%). The MIC50 was consistent for azithromycin among the 7 genotypes, but varied by 1 - 4 folds for doxycycline, ofloxacin and roxithromycin. Conclusions Clarithromycin, doxycycline and azithromycin exhibit an excellent activity against Ct, and the activity of azithromycin is consistent among the 7 genotypes of Ct.

Objective To establish the methods for measuring the dose to occupational staff's eye lens in interventional procedures with direct-reading dosimeters,and to realize the real-time monitoring of eye lens dose and warning for high dose rate,thus providing the scientific basis of the staff radiological protection in interventional procedures.Methods Direct-reading dosimeters were calibrated with personal dose equivalent HP (3).The eye lens doses for occupational staff in different kinds of interventional procedures were measured by the devices with both single-and double X-ray tubes.The data obtained fromthe direct-reading dosimeters was compared to those obtained from TLDs.Results Direct-reading dosimeters showed good linear fitting with the calibration of HP (3),and the coefficients of variation were lower than 5％.The average eye lens HP (3) for the main operator in coronary arteriography and stent implantation in brain obtained by direct-reading dosimeters were 12.0 and 24.5 μSv,respectively,whereas those obtained by TLDs were 11.9 and 22.7 μSv,respectively.The direct-reading dosimeters gave similar t~nds as TLDs do so.The direct-reading dosimeters were able to provide eye lens HP (3) in each individual interventional procedure,and to monitor the real-time dose rate as well.Conclusions The calibration of HP (3) and the data gained by direct-reading dosimeters are reliable.Therefore,the methods for real-time measurement of eye lens dose for occupational staff in interventional procedures are successfully established.

Objective To explore the treat methods in severe head injury (SHI) with brain herniation.Methods 73 patients suffered from SHI with brain herniation, who were undertaken system therapy in every different phase of post-trauma, were analyzed retrospectively.Results There were 17 patients died and 14 patients was GOS Ⅴ grade.Conclusion The systematic therapy in every different phase can enhance the synthetic therapeutic level of SHI and reduce the mortality and elevate the quality of life.

Objective To clone ,construction ,express and purify Tp47 of Treponema pallidum (Tp) ,and assess the immunoreac‐tivity by Western‐Blot .Methods Tp47 was amplified by polymerase chain reaction ,and then cloned to the vector pGEX‐6P‐1 .The correct sequence of the recombinant plasmids pGEX‐6P1‐Tp47 was transformed into Escherichia coli BL21 (DE3) and induced .The expression product was analyzed by sodium dodecyl sulfate polyacrylamide gel electropheresis and Western‐Blot .The expression protein was purified .Serum of different clinical stages of syphilis was used as the antibody to detect the immunoreactivity of the protein by Western‐Blot .Results A fusion protein with molecular weight about 71 × 103 was attained .Western‐Blot proved that the recombinant protein can react with Tp IgG positive sera .And the specificities and sensitivities of the diagnostic reagent detected by sera were 100% .Conclusion The recombinant protein Tp47 was expressed and purified with good antigen activity ,which could provide the basis of theory and practice for the development of early diagnostic kit applying to detect Tp infection .