1.Enhancement of CEA specific RNAi on gene engineering adenovirus H101 for treatment of tumor tissue of nude mice transplanted with human esophageal cancer cells
Hong ZHENG ; Guoqiang ZHAO ; Hongyan YANG ; Jimin ZHAO ; Yaohe WANG ; Ziming DONG
Basic & Clinical Medicine 2006;0(08):-
Objective To study the effect of gene engineering adenovirus H101 for treating esophagus carcinoma EC9706 induced by CEA gene specific silencing and to explore internal influential factor of H101 sensitivity. Methods To construct the siRNA expression vector of CEA and to inhibit the expression of CEA through RNA interference and gene transfection in EC9706 cell,stable CEA gene silencing system was set up,compared with empty vector group and non-transfected EC9706 cell,the model of athymic mouse subcutaneous transplantation tumor of human esophagus carcinoma EC9706 cell was established followed by injection with H101. The mRNA and protein expressions of CEA were detected by real time PCR and immunohistochemistry,the tumor size was measured. Results Silencing CEA gene by applying RNAi can inhibit CEA mRNA and protein expression in nude mice model with transplanted human esophageal cancer cells,there was no evident influence on tumor growth and mass oftumor. After using H101,the tumor size of interfering group was much smaller than that of empty vector group and normal control group(P
2. Expression of microtubule actin cross-linking factor 1 in gastric carcinoma and its effect on cell invasion and metastasis
Yongxu JIA ; Zhiwei CHANG ; Hong TANG ; Huijie FAN ; Yaohe WANG ; Yanru QIN
Chinese Journal of Digestion 2019;39(9):619-625
Objective:
To explore the role of microtubule actin cross-linking factor 1(MACF1) in the metastasis of gastric cancer.
Methods:
From 2009 to 2012, at The First Affiliated Hospital of Zhengzhou University, the paraffin blocks of gastric cancer and normal tissue adjacent to cancer of 107 patients who received radical gastrectomy were collected. The expression of MACF1 in tissues at protein level was detected by immunohistochemical staining. In 2017, at The First Affiliated Hospital of Zhengzhou University, fresh specimens samples of gastric cancer and normal tissue adjacent to cancer of 42 patients who received radical gastrectomy were also collected. The expression of