Objective To explore the relationship between personality and mental status of inpatients in the department of neurology of general hospital.Methods 150 inpatients in the department of neurology had been surveyed by Eysenck Personality Questionnaire(EPQ) and Symptom Checklist-90(SCL-90) with the domestic norm as control.Results The examining results of the inpatients in the department of neurology were as following.The scores of neurotisim(N) of EPQ were higher than that of the domestic norm(P<0.05);the scores of extra-introversion(E) and psychoticism(P) of EPQ were lower than that of the domestic norm(P<0.05).The scores of 7 factors including total scores,somatisation,depression,anxiety,hostility,panic and psychosis in SCL-90 were higher than that of the domestic norm( P<0.05).The N scores of EPQ was positively correlated with all factors of SCL-90,the E scoers of EPQ was positively correlated with hostility and paranoia factor of SCL-90,the P scores of EPQ was positively correlated with paranoia factor of SCL-90.Conclusion The mental characteristics of inpatient in the department of neurology of general hospital in acute period are that the incidence of somatisation,depression,anxiety,panic and psychosis are higher than normal people,compulsion and paranoia are as well as normal people.The character of neurotisim and introversion may be the personality background of it.

Objective To evaluate the effects of TGF ? 1, EGF, bFGF, KGF on the cell growth of androgen independent prostate cancer cell line PC 3M in vitro. Methods We cultured androgen independent prostate cancer cell line PC 3M in vitro and added TGF ? 1, EGF, bFGF, KGF to the medium at different concentrations of 1, 5,10 and 50 ng/ml. Brdu incorporation method was used to determine the status of cell proliferation. Results The absorbance ( A ) value of the control group PC 3M was 0 759. Seventy two hours after adding the growth factors separately, the inhibition was enhanced with the increasing of concentrations. At the dosage of 50 ng/ml, A value decreased to 0 400(TGF ? 1),0 455(EGF),0 532(bFGF),and 0 491(KGF),respectively, showing significant difference compared to the controls. When the mixture of 1 ng/ml EGF with 0 5 ng/ml or 5 ng/ml TGF ? 1 was added into the medium, A values were 0 522 and 0 299, respectively. Conclusions All these four growth factors inhibited the proliferation of PC 3M cell line in a dose dependent manner. TGF ? 1 and EGF have no interaction to the cell proliferation.

Objective:To Study the equity of Chinese health systems performance and provide evidence for strengthening health system.Methods:Based on WHO health systems performance evaluation framework,the provincial data of expanded pilots under the national health service investigation and relative concentration index (RCI) were used to study the inequality of Chinese health systems performance from regions,income and education,etc.Results:The RCI values were lower than 0.1 on health status and satisfaction dimensions.The RCI of financial risks protection was higher than 0.2,which was at a respective higher level.Conclusion:The health performance of people with lower education and wealth were fragility and these people usually with health shocks.Therefore,the national and regional government should pay more attention on vulnerable group and provide more healthcare management and security support.

Objective To study the feasibility and efficacy of operative method of maximal thymectomy by video-assisted thoracoscopic surgery(VATS)for myasthenia gravis(MG).Methods From August 2005 to June 2007,27 patients with MG received maximal thymectomy by VATS(VATS group).The thymus and fat tissues in the anterior and upper mediastinum were resected.Patients who received transsternal thymectomy from May 2004 to July 2005 served as a control(conventional group).Results In the VATS group,the maximal thymectomy by VATS was completed in 26 patients,one patient was converted to open surgery because of the injury to the brachiocephalic vein.Compared with the conventional group,the patients in the VATS group had fewer blood loss [(46.0?5.7)ml vs(120.0?18.8)ml,t=-19.231,P=0.000],lower level of analgesic requirement(3 vs 12,?2=7.068,P=0.008),and shorter postoperative hospital stay [(7.0?1.2)d vs(11.0?2.5)d,t=-7.379,P=0.000].There was no significant difference in the operation time,myasthenia gravis crisis,and surgical outcomes between the two groups.Conclusions Maximal thymectomy by VATS is feasible and safe for patients with MG.Patients have shorter hospitalization,less surgical trauma and pain,lower rate of complication,and quicker recovery after the treatment.Further studies are needed to investigate its long-term efficacy

BACKGROUND: Few reports concern the effects of dendritic cells-a kind of antigen presenting cells, and interleukin-10 (IL-10) on airway hyperreactivity or inflammation. OBJECTIVE: To construct mice IL-10 recombinant adenovirus vector Ad-mIL-10 to acquire the dendritic cells modified by mIL-10, which can provide a foundation for the further study. METHODS: Mouse IL-10 (mIL-10) gene comprise of enzyme cutting spot was synthesized according to the mIL-10 gene sequence and multiclone spot of adenovirus vector, connected to pMD18-T vector and sequenced. MIL-10 was subcloned to BD Adeno-X~(TM) vector, packed and augmented in HEK 293 cells, following determine the protein expression, and the vector was transfected to mice bone marrow-derived dendritic cells. RESULTS AND CONCLUSION: Recombinant adenovirus vector Ad-mIL-10 was successfully synthesized, packed and augmented, which could highly express protein IL-10. Bone marrow-derived dendritic cells were successfully cultured and transduced in vitro. It suggested that it is feasible to transfect mice dendritic cells by Ad-mIL-10 adenovirus vector. The study can provide more sufficient theoretic evidence for the possibility of correlative gene therapy.

Thirty six patients aged 50-70 years with benign prostatic hyperplasia (BPH) received doxazosin and desmopressin treatment for 4 weeks.After treatment the International Prostate Symptom Scores (IPSS) and Quality of Life (QoL) scores of patients were significantly improved (17.4 ± 2.5 vs.11.8 ± 1.1 and 3.9 ±0.6 vs.1.8 ± 1.3,respectively;both P ＜ 0.01).The nocturia frequency was decreased from 4.6±1.5 to 2.1±1.6 (P＜0.01);nocturnal urine output from (564±73) to (348±45) ml (P＜0.05);the hours of undisturbed sleep increased from (88.6 ± 17.5) min to (146.2 ± 12.8) min (P ＜0.05).There were no significant differences in serum levels of sodium,potassium and chlorine,and osmoticpressure before and after treatment.The results indicate that doxazosin combined with desmopressin is safe and effective for treatment of nocturia in patients with BPH.

Objective Based on the analysis of relevant factors on scores of the first clinical dietitian post-training examination (CDPTE) in China,to explore the clinical dietitians' post competency evaluation basis.Method 108 students who completed the clinical nutritionist training (60 physicians,nurse or technician 48) were imposed comprehensive evaluation designed according to the concept of post competency.Through analysis and comparison,the correlation factors of the candidates' passing rate and their mastering rate of the module were studied.Results The results of all the candidates' comprehensive theoretical examination increased with the degree and the source of the candidates.Among them,the college students' pass rate was 76.47％,undergraduates' pass rate was 86.21％,Graduates' pass rate was 96.97％;the pass rate in western region was 85％,the central part was 85.71％,the eastern part was 89.55％.All the candidates' knowledge module mastery rate in the comprehensive examination of the theory from high to low in order was:for hospital diet (73.7％),enteral nutrition and parenteral nutrition (72.7％),public nutrition (70.7％),nutrition screening and assessment (66.7％),common nutrition related diseases (65.4％),clinical nutrition related health students regulations,medical psychology and ethics basic knowledge (40.0％).The examination pass rate was related to the educational level of the examinee and the source area,while the knowledge module mastery rate was closely related to the work of clinical nutrition.Conclusion We concluded that the CDPTE could objectively reflect the candidate's clinical competence and professionalism and it was designed on the basic principle of post competency.CDPTE has a positive significance for scientific assessment of clinical dietician,guide for training,and evaluation of training effects as well.The scores of CDPTE can objectively reflect the examinees' clinical competence and professionalism and CDPTE can achieve the goal of evaluating the candidates' competency,and it is of practical significance for scientific evaluation of clinical practice,guiding learning and evaluating the training effect.

Objective To evaluate the diagnostic and therapeutic status of lower urinary tract symptoms (LUTS) of benign prostatic hyperplasia (BPH) in urological outpatients.Methods The survey was conducted at 57 urological clinics in 14 cities during the period of October-December 2011.All surveyed male outpatients were inquired about whether there was a history of LUTS/BPH or not.Then eligible patients received a more detailed questionnaire for LUTS.Results Among a total of 6 200 male outpatients,47.4％ (n =2 940) had a history of LUTS/BPH.The rate for LUTS was 30.0％ (n =882) in patients aged under 40 years and 60.0％ (n =1 764) in those aged over 60 years.The major complains included frequency (72.0％),urgency (53.0％) and nocturia (48.0％).At a rate of 63.0％,the patients aged 71-80 years came more to a hospital for nocturia than 61-70 years (52.0％) and 51-60 years (49.0％).The average International Prostate Symptom Score (IPSS) was 14.98 (6-35) and 59.0％ patients had moderate symptoms and 26.0％ severe symptoms.The average score of quality-of-life was 4.12 (1-6) points.And the average overactive bladder symptom score (OABSS) was 5.78 (3-15) points and 56.0％ patients had moderate overactive bladder (OAB) and 3.0％ severe OAB.Urinalysis was largely normal.The main causes for LUTS included OAB (25.2％),BPH and OAB (20.4％),BPH (20.2％),prostatis (20.2％) and urinary infection (11.8％).Conclusions LUTS and BPH are common in urological outpatients.And the incidence is rising yearly.Clinical examinations and drug regimens should be timely adjusted according to the etiology.And patient education should be strengthened at the same time.

Objective To evaluate the effect of hydrodistension on interstitial cystitis and the psychological changes after hydrodistension.Methods Thirty two patients with interstitial cystitis underwent hydrodistension from May 2011 to Jun 2012 in Department of Urology,Beijing Hospital.Interstitial cystitis symptom index(ICSI),Visual onalogue Scale (VAS) pain score,quality of life questionnaire(QOL) score,self-rating anxiety scale(SAS) score,self-rating depression scale(SDS) score were recorded and analyzed before and at 1-3 month after hydrodistension surgery.The voiding diary and postoperative complications were cared.Results Symptoms were improved in twenty nine patients,the efficiency rate was 90.6％ (29/32).There were significant differences in ICSI score,VAS pain score,QOL score,SAS score,SDS score between pre and 3 month post-surgery [(26.7± 2.3) vs.(15.2±1.9),(4.6±2.5) vs.(1.8±1.1),(4.3±2.2) vs.(2.2±1.6),(53.8±3.7) vs.(40.8±2.3),(54.8±2.3) vs.(42.4±1.5),t=8.33,7.94,6.54,7.20,8.52,respectively,all P ＜0.05].No complication was found after surgery.Both the clinical symptoms and the anxiety and depression symptoms in patients were relieved,and QOL was improved.Conclusions For the patients with interstitial cystitis,the undergoing of hydrodistension surgery can relieve the anxiety and depression symptoms and improve quality of life.

Objective To explore the clinical effects of tension-free vaginal tape obturator system inside-out (TVT-O) for the treatment of female stress urinary incontinence.Methods A retrospective analysis of 102 patients with stress urinary incontinence who were performed TVT-O surgery in our hospital from October 2011 to June 2012.The postoperative follow-up time was 1 to 8 months.Results 102 patients with stress urinary incontinence were effectively controlled after surgery,the subjective satisfactory rate was 96.1％ (98 cases),the objective of efficiency was 93.1％(95 cases).After a mean follow-up of 1-8 months,no symptoms were in recurrence.The average surgery time was (35.0± 2.1) min and the average blood loss was (25.0± 6.4) ml.International consultation on incontinence questionnaire short form (ICIQ-SF) score was 16.2 ± 3.4 beforc operation and reduced to 8.7±2.6 after 4 weeks (P＜0.05).Incontinence quality of life questionnaire score (I-QOL) preopcratively was 40.2±19.5,and 88.7±12.3 after 4 weeks (P＜0.05).One hour urine pad test:the mean preoperative urine leakage was (27.6±8.2) g,and became (5.5± 1.3)g 4weeks after operation (P＜0.05).Two patients had dysuria 3 days after surgery and became recovery after keeping catheterization one week.Eight patients had groin thigh pain which remitted two days to one week later.One patient was diagnosed overactive bladder firstly and three patients had suffered current urinary infection after surgery.Conclusions TVT-O is an effective treatment method for female stress urinary incontinence because of its high level of standardization,small trauma,few complications and fast recovery.