Objective:To explore the relationship between ventilator-associated pneumonia (VAP) and neutrophil/lymphocyte ratio (NLR) before mechanical ventilation in patients with malignant tumors.Methods:A retrospective nested case-control study was conducted. Patients with malignant tumor treated by mechanical ventilation admitted to the Third Affiliated Hospital of Guizhou Medical University from February 2015 to February 2020 were enrolled. The patients with VAP were selected as the case group, and the matched non-VAP cases were selected according to 1∶2 as the control group. The clinical data were collected, and the differences of each index between the two groups were compared. The influencing factors of VAP in patients with malignant tumor were analyzed by multivariate Logistic regression.Results:During the study period, 1 271 patients with malignant tumors were treated with mechanical ventilation, of which 241 cases had VAP, and the incidence of VAP was 18.96%. There were 232 VAP patients in the case group matched 464 non-VAP patients in the control group. The clinical data of age, gender, hospitalization diagnosis, primary tumor, regional lymph node and distant metastasis (TNM) stage, acute physiology and chronic health evaluation Ⅱ (APACHEⅡ), complications, duration of mechanical ventilation, hemoglobin (Hb) and serum albumin (Alb) levels were balanced and comparable between the two groups, and the cluster nursing measures were consistent. Compared with the control group, although there was no significant difference in neutrophil count (NEU) and lymphocyte count (LYM) in the case group [NEU (×10 9/L): 3.81±1.07 vs. 3.64±1.05, LYM (×10 9/L): 2.06±0.59 vs. 2.15±0.62, both P > 0.05], NLR was significantly increased (2.07±1.05 vs. 1.89±0.96, P < 0.05), and the hospital stay was significantly longer (days: 24.84±3.81 vs. 13.19±3.98, P < 0.01). NLR, gender, age, APACHEⅡ score, TNM stage, Hb, serum Alb and duration of mechanical ventilation were included in multivariate Logistic regression analysis. The results showed that patients with elevated NLR had higher risk of VAP [odds ratio ( OR) = 1.187, 95% confidence interval (95% CI) was 1.015-1.387, P = 0.032]. In patients with VAP, NLR was negatively correlated with the time of mechanical ventilation before VAP (r = -0.327, P = 0.000), and positively correlated with the time of treatment with antibiotics after VAP (r = 0.559, P = 0.000). Conclusion:Elevated NLR in patients with malignant tumors who were on mechanical ventilation can significantly increase the risk of VAP and increase the difficulty of treatment.

Objective To analyze risk factors of multiple drug-resistant infections in neonatal intensive care unit (NICU). Methods The clinical data from 284 hospitalized pediatric patients were retrospectively analyzed from June 2011 to July 2015 . The differences between 59 cases with multiple drug-resistant infections and 225 cases with non-multiple drug-resistant infections were compared and analyzed by logistic regression. Results All of 284 cases were single birth. Fifty-nine cases ( 13 . 13 ± 9 . 03 days old) had multiple drug-resistant infections, in which 42 were males and 17 were females. Two hundred and twenty-ifve cases ( 14 . 21 ± 8 . 34 days old) had non-multiple drug-resistant infections, in which 175 cases of males and 50 cases of females. Single factor analysis showed that 8 factors, including gestational age, birth weight, days in hospital, Apgar score at birth, mechanical ventilation, parenteral nutrition, and the categories and duration of use of antimicrobial agents, were the risk factors of multiple drug-resistant infections (P?

Objective To analyze the risk factors of multiple drug resistant bacterial infections in patients with chronic obstructive pulmonary disease (COPD), and provide guidance for disease control and prevention. Methods Clinical data of 814 COPD patients were retrospectively analyzed from June 2011 to May 2015, including patient's age, gender, smoking history, age of onset, severity, aggravated frequency, duration of exacerbations, diabetes mellitus, complications, use frequency and use duration of glucocorticoid, use frequency of antimicrobial agents and use duration of each time, types of antimicrobial drugs used, combined with antibacterial drugs, plasma albumin concentration, blood glucose, bacteria culture detection of multi drug resistant bacteria infection. The risk factors of multi drug resistant bacteria infection were analyzed. Results A total of 857 pathogenic bacteria were isolated from 814 COPD patients with pulmonary infection. Multiple drug resistant bacteria infection were detected in 170 cases, and 175 strains (20.42%) were detected. The detection rate of multi drug resistant/PAN resistant pseudomonas aeruginosa (MDR/PDR-PA) was 55.38% (36/65). There were significant differences in patients with multi drug resistant bacteria infection between different clinical pathological characteristics. Logistic regression analysis showed that the acute exacerbation duration (days), long time use of antimicrobial drugs, and high frequency of corticosteroids and antibiotics use were independent risk factor of multi drug resistant bacteria infection in COPD patients. Conclusion Prevention and treatment of multiple drug resistant bacteria infection in COPD patients should pay attention to the combination of community and hospital, and take effective measures to prevent and control the risk factors.

Objective To analyze the risk factors for healthcare-associated pneumonia (HAP) in patients with orthopedic injury,provide the basis for making prevention and control measures.Methods HAP occurred in patients with orthopedic injury and admitted to the department of orthopedics of a hospital from June 2011 to May 2015 were investigated retrospectively,risk factors were analyzed by univariate and multivariate logistic regression methods.Results A total of 2 578 patients with orthopedic injury were investigated,92 patients developed HAI,incidence of HAP was 3.57％.107 strains of pathogens were detected,the major were Klebsiella pneumoniae (n =22,20.56％),Escherichia coli (n =14,13.08％),and Acinetobacter baumannii (n =13,12.15％).Risk factors for HAP in patients with orthopedic injury were length of hospital stay≥15 days,smoking history≥3 years,bedridden ≥7 days,associated with underlying diseases,complications,indwelling catheter≥7 days,surgical operation,mechanical ventilation,admitted to intensive care unit,open injury,blood sugar≥11 mmol/L,plasma albumin＜30 g/ L,hemoglobin concentration＜90 g/L,and use of glucocorticoid≥4 days (all P＜0.05).Multivariate logistic regression analysis showed that smoking,bedridden,surgery,mechanical ventilation,glucocorticoid use,and anaemia were independent risk factors for HAP in patients with orthopedic injury.Conclusion The occurrence of HAP in patients with orthopedic injury is related with multiple factors,the major are surgical operation,mechanical ventilation,glucocorticoid use,long term smoking,bedridden,and anaemia.

Objective:To analyze the related factors for plural bacterial infection in elderly patients with health care-associated pneumonia(HCAP), in order to provide the reference for clinical practice.Methods:A total of the 1 012 elderly patients conforming to inclusion criteria were in the age of 60-87(70.7±6.2)years, with 431 cases of males and 581 cases of females.The clinical data of elderly patients with HCAP admitted to our hospital from February 2015 to December 2018 were collected.According to the number of infected bacteria category, the patients were divided into the single bacterial infection group and the plural bacterial infection group.The distribution of pathogens was compared between the two groups, and the related factors for plural bacterial infection were analyzed.Results:There were 122 HCAP cases in the plural bacterial infection group, with 286 strains of pathogenic bacteria detected, while 890 HCAP cases were found in the single bacterial infection group, with 890 strains of pathogenic bacteria detected.Compared with the single infection group, the plural bacteria infection group showed that the proportions of Gram-positive Staphylococcus aureus and Enterococcus faecium were increased, while the proportion of Staphylococcus epidermidis was relatively decline( χ2=11.086, 8.460 and 4.056, P=0.001, 0.004 and 0.044). The proportions of Gram-negative Pseudomonas aeruginosa and Stenotrophomonas maltophilia were higher, while the proportions of Escherichia coli and Klebsiella were lower in the plural bacteria infection group than in the single bacteria infection group( χ2=7.495, 4.918, 9.011 and 4.604, P=0.006, 0.027, 0.003 and 0.032). Multivariate Logistic regression analysis showed that the independent risk factors for plural bacteria infection in elderly HCAP patients were the combined use of antibiotics within 30 days(≥3 kinds), more chronic underlying diseases(≥2 kinds), pneumonia severity index(PSI)classification being high, longer hospitalization time within 90 days(≥15 days), a history of ICU stays within 90 days, age(≥70 years old)( OR=2.389, 1.840, 1.289, 1.877, 2.089 and 1.981, P=0.001, 0.003, 0.001, 0.002, 0.001 and 0.002, respectively). Conclusions:The plural bacteria infection in elderly HCAP patients is related to many factors.The effective measure to reduce the plural bacteria infection in elderly HCAP patients are to focus on patients with severe disease, more basic diseases and advanced age, and on the shortening of the unnecessary hospitalization time, the reducing of the time of ICU stays, the reasonably selecting of antibiotics, the reducing of the unnecessary combination of antibacterial drugs.

Objective:To investigate factors related to bloodstream infections in patients with catheter-associated urinary tract infections(CAUTI)aged over 80 years.Methods:Clinical data of patients with CAUTI aged 80 years in our hospital from August 2014 to September 2019 were retrospectively analyzed.Independent and relevant factors for bloodstream infections in patients were analyzed by using univariate and multivariate methods with SPSS20.0 statistical software.Results:There were 138 patients with bloodstream infections, giving an infection rate of 9.28%.Univariate and multivariate analysis showed that the timing of catheterization(delayed or no extubation after infection), urinary tract operation, glucocorticoid use, tumor chemotherapy, serum albumin concentration reduction, blood glucose and multi-drug resistant bacterial infection were independent risk factors for bloodstream infections in patients with CAUTI aged over 80 years.Conclusions:Early extubation, blood glucose control, correction of hypoproteinemia, reduction of multi-drug resistant bacterial infection, rational use of glucocorticoids and tumor chemotherapy, and heightened attention to patients undergoing urinary tract surgery can reduce the risk of bloodstream infections in patients with CAUTI aged over 80 years.

Genome editing tools such as the clustered regularly interspaced short palindromic repeat (CRISPR)-associated system (Cas) have been widely used to modify genes in model systems including animal zygotes and human cells, and hold tremendous promise for both basic research and clinical applications. To date, a serious knowledge gap remains in our understanding of DNA repair mechanisms in human early embryos, and in the efficiency and potential off-target effects of using technologies such as CRISPR/Cas9 in human pre-implantation embryos. In this report, we used tripronuclear (3PN) zygotes to further investigate CRISPR/Cas9-mediated gene editing in human cells. We found that CRISPR/Cas9 could effectively cleave the endogenous β-globin gene (HBB). However, the efficiency of homologous recombination directed repair (HDR) of HBB was low and the edited embryos were mosaic. Off-target cleavage was also apparent in these 3PN zygotes as revealed by the T7E1 assay and whole-exome sequencing. Furthermore, the endogenous delta-globin gene (HBD), which is homologous to HBB, competed with exogenous donor oligos to act as the repair template, leading to untoward mutations. Our data also indicated that repair of the HBB locus in these embryos occurred preferentially through the non-crossover HDR pathway. Taken together, our work highlights the pressing need to further improve the fidelity and specificity of the CRISPR/Cas9 platform, a prerequisite for any clinical applications of CRSIPR/Cas9-mediated editing.
Blastocyst ; CRISPR-Cas Systems ; Hemoglobins, Abnormal ; genetics ; metabolism ; Humans ; Zygote