As important adhesion molecule on cell surface,integrin is a kind of important receptor family. It′s im- portant role is to mediate intercellular adhesion and adhesion between cells and extracellular matrix,also regulate proliferation,differentiation,migration of cells and tissue remodeling,and plays an important role in maintaining cellular form and growth.The present article made a short review about research progress of integrin β1 effect in cardiovascular diseases in recent years.

In China,Chinese herbs have been used to promote health status and prevent diseases for a long history.Most of the herbs are administered orally,then metabolized and absorbed in the gastrointestinal tract.After they enter gastrointestinal tract,the herbs meet gastrointestinal bacteria and exert effects on microbial community,resulting in affecting the health status of animals.This paper summarized the effects of Chinese herbs on gastrointestinal bacteria.

Some problems, such as the differences of intelligence and knowledge basis among the students, ambiguity of teaching goal and lack of proper textbooks for instrumental analysis as well as "double-teacher", should be solved urgently. The authors try to find out some solutions to them.

By studying medical works during the Sui and Tang dynasty,we discussed the factors that doctors of that period believed to affect the dosage of medicine.We found that doctors of that period developed the dosage theory in terms of compatibility of medicines,onset time a disease,dosage form of medicine,constitution and age of patients,and toxicity of medicine.

BACKGROUND:It is controversial whether bone marrow mobilization can retain in cardiac injured position in congestive cardiomyopathy or differentiate into cardiomyocytes and vascular endothelial cells.
OBJECTIVE:To study the effects of granulocyte colony stimulating factor (G-GSF) on myocardium and angiogenesis in rats with congestive cardiomyopathy.
METHODS:Fifty Wistar rats with heart failure caused by adriamycin-induced cardiomyopathy were divided into heart failure group (n=20) treated with normal saline and bone marrow mobilization (n=30) treated with subcutaneous injection of recombinant human G-GSF. Ten rats from the bone marrow mobilization were kil ed at day 6 of mobilization, and myocardial tissue was taken for CD43 immunofluorescent staining. Blood samples were taken from the rat tail in each group before and 5 days after treatment to count total number of white blood cells and percentage of mononuclear cells. Meanwhile, mononuclear cells extracted from the peripheral blood were used for flow cytometry detection. At day 5 after treatment, bromodeoxyuridine (BrdU, 50 mg/kg) was successively given to al rats for 4 weeks before they were sacrificed. Myocardial tissues were taken to determine the homing of mononuclear cells and evaluate differentiation of mononuclear cells into cardiomyocytes and vascular endothelial cells using BrdU staining, BrdU/myosin heavy chain double staining, and BrdU/actin double staining. Hematoxylin-eosin staining was used for determination of blood vessel density.
RESULTS AND CONCLUSION:G-CSF mobilization increased the number of mononuclear cells that was significantly higher than before treatment (P<0.05). Flow cytometry showed that the number of CD34-positive mononuclear cells in the peripheral blood was higher in the bone marrow mobilization than in the heart failure group (P<0.05). Myocardial CD34 immunofluorescence showed that the heart failure group was negative and the bone marrow mobilization group was positive. In the bone marrow mobilization group, the myocardial tissue was positive for BrdU staining, BrdU/myosin heavy chain double staining and BrdU/actin double staining, while vascular endothelial cells in the region of myocardial injury was positive for BrdU;conversely, the heart failure group was negative. The density of blood vessels in the bone marrow mobilization group was significantly higher than that in the heart failure group (P<0.001). These findings indicate that bone marrow mobilization increases the number of mononuclear cells, and these cells are homing to myocardial injury, thereby playing a repair role in the myocardium and vascular tissue of heart failure rats with congestive cardiomyopathy.

Objective To study the molecular mechanism of benzo( a) pyrene underlying the role of estrogen metabolism-related genes in human endometrial cancer cells. Methods Effect of benzo( a) pyrene on CYP450 was studied by exposing human endometrial cancer cells ( RL95-2) to benzo( a) pyrene at various concentrations ( 0. 016,0. 8,0. 4,2. 0 and 10 ?mol/L BP) for 12 h,or to 2 ?mol/L BP for 6,12,24,36 and 48 h. RL95-2 cells were harvested and cleaved. Expressions of CYP1A1,CYP1B1 and CYP3A4 proteins in RL95-2 supernatant were detected by Western blotting. Supersome in expressed CYP450 was used as a positive control to confirm the reliability of assay methods. Results The expression of CYP1A1 protein was significantly increased in RL95-2 cells 12 h after they were exposed to different concentrations of benzo( a) pyrene or to 2 ?mol/L BP for different periods of time,and reached it peak at 48 h,indicating that expression of CYP1A1 protein depends on the used dose and time of benzo( a) pyrene. Conclusion Benzo( a) pyrene can increase the expression of CYP1A1 in human endometrial cancer cells,cause changes in estrogen metabolism,and inhibit proliferation of human endometrial cancer ells.

Chronic Disease ; Forkhead Transcription Factors ; Hepatitis, Viral, Human ; Humans

Objective To establish a pretreatment method which is applicable to the determination of strontium(Sr)in toothpaste by flame atomic absorption spectrometry.Methods The pretreatment was performed by using digestion pot technique, with lanthanum nitrate as the anti-interference agent.Flame atomic absorption spectrometry was used in this study,the parameters of digestion temperature and the results of two pretreatment methods were compared.Results The toothpaste sample was digested completely by using digestion pot technique with little examined elements lost during digestion.Time-saving and pollution-free were seen in the test.The detection limit of this method was 0.02?g/ml,and the RSDs were 0.98%-3.01%.The recovery rate was at the range of 94.7%-110%.No significant difference was seen between these two pretreatment methods.Conclusion This method has advantages of high sensitivity and precision in the determination of strontium in toothpaste by flame atomic absorption spectrometry,so it can be employed in various laboratories.

Objective:To study the influence of DiKang capsule on expression of TGF? receptor Ⅱ on hepatic stellite cells(HSC)in rat with liver fibrosis introduced by DMN.Methods:TGF? receptor Ⅱ on HSC in rat with liver fibrosis introduced by DMN were measured by FCM.Results:In group of 10 ?g/kg DMN injected 3 weeks,expression of TGF? recptor Ⅱ on HSC in these rat fed with DiKang capsule 3 weeks were higher than that not feeding capsule.But in group of injecting 5 ?g/kg DMN continuous 6 weeks,expression of TGF? receptor Ⅱ on HSC does not changed with feeding or not feeding DiKang capsule.Conclusion:DiKang capsule may have a certainly role on expression of TGF? receptor Ⅱ on HSC in rat with liver fibrosis introduced by DMN.

Objective To evaluate the efficiency of lamivudine combined with hepatitis B immunoglobulin to prevent hepatitis B recurrence after liver transplantation.Methods The literature concerning the application of lamivudine and hepatitis B immunoglobulin after liver transplantation was collected.The efficacy of initial lamivudine,and hepatitis B immunoglobulin alone or combined together was evaluated in liver transplantation recipients with hepatitis B by performing a systematic review of the literature with a Meta-analysis of clinical trials.Odds ratio(OR)was applied to evaluate the effect of therapeutic alliance to decrease the reinfection rate whether or not.Results We identified 7 clinical trials,and there were 360 patients subjected.OR and 95% confidence interval(95%CI)was 0.34(95%CI ranging from 0.18 to 0.64).For overall test result,Z value was 3.33 and P value was 0.01.The P value was 0.310 for our test of study homogeneity.Conclusion Our meta-analysis shows that lamivudine or hepatis B immunoglobulin can effective prevent hepatitis B recurrence after liver transplantation,and therapeutic alliance is more effective than monotherapy,and tolerance to lamivudine or hepatis B immunoglobulin was good.