1.Effects of vitamin C on the DNA of liver cells of the rats fed with low selenium and high cadmium fodder.
Yao-kui DUAN ; Wen-hua CAO ; Ai-guo LI
Chinese Journal of Applied Physiology 2006;22(3):332-342
Animal Feed
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Animals
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Ascorbic Acid
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pharmacology
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Cadmium
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analysis
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DNA
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analysis
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Hepatocytes
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chemistry
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Rats
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Rats, Wistar
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Selenium
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analysis
2.Epidemiological characteristics of co-infection of hepatitis C virus among newly reported HIV infeced patients in Chinese and Burmese from 2016 to 2017 in Dehong Prefecture of Yunnan Province
Ji-bao WANG ; Xiao-chen CHEN ; Xing DUAN ; Yi-kui WANG ; Jin YANG ; Tao YANG ; Run-hua YE ; Yue-cheng YANG ; Shi-tang YAO ; Song DUAN ; Na HE
Chinese Journal of Disease Control & Prevention 2019;23(10):1293-1296,1300
Objective To understand the prevalence and epidemiological characteristics of hepatitis C virus (HCV) co-infection among newly reported HIV-infected patients from 2016 to 2017 in Dehong Prefecture of Yunnan Province. Methods A cross-sectional survey was conducted to detect HCV antibodies in 2 196 newly reported HIV-infected persons in Dehong Prefecture, Yunnan Province, from 2016 to 2017. Results A total of 694 cases(31.6%) were detected antibodies against HCV during HIV-infected persents. The prevalence of HCV co-infection among newly reported HIV-infected patients in Chinese and Burmese was 14.4% (112/780) and 41.1% (582/1 416), respectively. Moreover, the prevalence of HCV co-infection was higher in male, ethnic minorities such as Dai and Jingpo minority, primary school and below and farmers in both Chinese and Burmese. Multivariate Logistic regression model showed that HCV infection was associated with male, Dai minority, HIV infection by injecting drug and famers among Chinese and Burmese reported HIV-infected patients. Conclusions The prevalence of HCV co-infection was high among newly reported in Dehong Prefecture of Yunnan Province. Meanwhile, the epidemiological characteristics of HIV/HCV co-infections in Chinese and Burmese are different, thus different prevention and control measures are needed for patients of different nationalities.
3. Myocardial specific overexpression of Sirt1 inhibits recovery of cardiac function in mice with myocardial ischemia
Tian-Tian LIU ; Jun-Ling CAO ; Qing-Qing WANG ; Jin-Long DUAN ; Jian-Guo LIN ; Kui-Wu YAO ; Kui-Wu YAO
Chinese Pharmacological Bulletin 2023;39(6):1036-1041
Aim To investigate the effect of overexpression of silent information regulator 1 (Sirtl) on cardiac function in mice with myocardial ischemia. Methods Myocardial specific Sirtl overexpression transgenic mice (Sirtl-Tg) and littermate control mice (C57BL/6J), half male and half female, were randomly divided into control sham operation group (Con), control model group (Con +ISO), Sirtl overexpression sham operation group (Sirtl-Tg) and Sirtl overexpression model group (Sirtl-Tg + ISO). Isoproterenol (ISO) was injected subcutaneously into the back of the neck at 100 mg • kg
4.Pharmacological mechanism analysis of oligopeptide from Pinctada fucata based on in silico proteolysis and protein interaction network.
Jin-Long DUAN ; Kui-Wu YAO ; Xiao-Xiao FENG ; Jue CAO ; Zhou YU
China Journal of Chinese Materia Medica 2017;42(17):3424-3429
Thoracic obstruction is mainly attributed to the scope of coronary heart disease in modern medicine, and traditional Chinese medicine(TCM) shows a significant effect in the treatment of thoracic obstruction. In this research, a network pharmacology method was carried out to systemically study the underlying mechanism of the core herbal compatibility in TCM on the thoracic obstruction. First, we collected the literature about TCM prescriptions for treating thoracic obstruction from CNKI. Then, a prescription database was establish by TCM inheritance support platform system(V2.5) to determine the medication rules and core herbal compatibility in TCM. Finally, to obtain the potential signaling pathways, KEGG pathway analysis was performed by BATMAN-TCM online analysis tool. Results showed that the potential signal pathway of core herbal compatibility in TCM for the clinical treatment of thoracic obstruction was calcium ion and cGMP-PKG signaling pathway. This study provided a new research strategy for the study of the medication rules and mechanism of traditional Chinese medicine in the treatment of thoracic obstruction.
5.Explore Effect of Jieyu Qutan Huazhuo Prescription on Gut-liver Axis of Rats with High-fat Diet Based on 16S rDNA Sequencing
Na LI ; Yang-yang WU ; Jin-long DUAN ; Xu-tong ZHENG ; Kui-wu YAO
Chinese Journal of Experimental Traditional Medical Formulae 2021;27(9):77-85
Objective:To study the effect of Jieyu Qutan Huazhuo prescription(JQHP) on the gut microbiota of rats with high-fat diet,and to explore the effect of Chinese medicine on the regulation of gut microflora and the restoration of gut-liver axis balance. Method:Seventy male SPF Wistar rats were randomly divided into a normal group of 10 and a model group of 60. Mice in the normal group were fed with normal diet and mice in the model group were fed high-fat diet. After 12 weeks,the model group was randomly divided into 6 groups with 10 animals in each group,namely the model group,Xuezhikang group,Liputuo group,and low,medium and high-dose groups of JQHP. The JQHP low-dose,medium-dose and high-dose rats were intragastrically daministered with 0.4,0.8,1.6 g·kg-1,respectively, rats in Liputuo group with Liputuo 2 mg·kg-1,rats in Xuezhikang group with Xuezhikang 0.1 g·kg-1. The rats in the normal group and the model group were intragastrically administered with the same amount of distilled water. Stool were collected after continuous gavaging for 8 weeks,16S rRNA gene sequencing was performed,blood was collected from the abdominal aorta to detect blood lipids,and the liver tissue and ileum tissue were collected for hematoxylin and eosin (HE) staining for pathomorphological observation. Result:Compared with the normal group,the total cholesterol (TC),triglyceride (TG),and low-density lipoprotein (LDL-C) in the model group were significantly increased,while the high-density lipoprotein (HDL-C) was decreased (