Objective To identify the stem cell characteristics of human diploid cells 2BS and evaluate the potential as biological function positive cells for quality detection of stem cells.Methods Flow cytometry was applied to detect the expression levels of stem cell surface markers in 2BS cells.The staining with alizarin red S,oil red O and alcian blue was used to evaluate the ability of osteogenic,adipogenic and chondrogenic differentiation,respectively.Co-culture with lymphocytes was used to observe the effects on lymphocyte proliferation.Results Human diploid cells 2BS expressed the surface markers CD73,CD 105 and CD90 of stem cells,and possessed the potential of osteogenic differentiation and inhibiting lymphocyte proliferation.Conclusion 2BS cells have several properties of stem cells and could be the positive control for surface markers detection of stem cells by flow cytometry.

IL-8 is a cytokine which belongs to the chemotactic superfamily.It is one of the major mediators in the inflammatory response.In recent years,it was found that it had multiple functions,such as chemoattractant,potent angiogenic,and so on.The cytokine is believed to play an important role in the pathogenesis of inflammation,tumor progress and immune disease.In this review,the properties of IL-8 and highlight its roles on inflammation and tumor progress were surveied.

Objective To explore the relationship between brain development and early behavior development of infant rats. Methods Infant Spraque - Dawley(SD) rats were used respectively for testing their spontaneous behaviors, inhibitory escaping response and behavior development, and also determine their brain weight and content of Zinc in different part of the brain at age of 1 day, 11 days and 21 days. Results There was a positive correlation between brain development and early behavior development of SD rat, and the high content of zinc in hippocampus and cerebellum. Conclusion It is suggested that the high content of Zinc guarantee furnish security for the late behavior development of infant rat.

The freshmen column in 39 985 Projectacademic libraries was investigated , which showed that 15 out of the 39 985 Projectacademic libraries have established freshmen column on their library website .Its posi-tion, contents and forms were analyzed.The typical freshmen column in libraries of Qinghua University, Shanghai Jiaotong University , and Sun Yat-sen University was described with suggestions put forward for improvement of its position, contents and forms.

Independent component analysis (ICA) aims at decomposing an observed random vector into statistically independent variables. Fast independent component analysis (FastICA) algorithm and its variants are catching more attention because of their simplicity and convergence speed. In this paper, a novel method referred to robust independent component analysis (RobustICA), based on normalized kurtosis and optimal step-size, is analyzed in detail. When applied to fetal electrocardiogram (FECG) extraction and compared with FastICA, it gave decent results and showed prosperous future usages.
Algorithms ; Electrocardiography ; Female ; Fetal Heart ; physiology ; Fetal Monitoring ; Fetus ; Humans ; Pregnancy ; Principal Component Analysis ; Signal Processing, Computer-Assisted

Objective To study the effects of acute hypervolemic hemodilution (AHH) with hydroxyethly starch 130/0.4 on function of blood clotting in patients undergoing spinal operation. Methods Thirty patients scheduled for spinal operation were randomly divided into AHH group and control group with 15 cases each. Patients in AHH group were performed AHH by transfusing hydroxyethly starch 130/0.4 (15 ml/kg) with 25 ml/min before skin incision, to achieve expansion of 20% to 30%. Patients in control group were transfused routinely. Blood samples were taken from internal jugular vein before anesthesia induction (T0), at 1 h after anesthesia induction (T1) and termination of operation (T2) for determination of hemoglobin (Hb), hematocrit (Hct), platelet (Plt), prothrombin time(PT), partial thromboplastin time ( APTT), thrombin time( TT) and fibrinogen( FIB), at the same time heart rate( HR), mean arterial pressure (MAP) and central venous pressure (CVP) were observed and recorded. Results HR and MAP in both groups had no statistical difference within or between groups (P > 0.05). CVP at T1 and T2 in AHH group [(11.8 ± 1.0,(11.3 ± 1.0) cm H2O (1 cm H2O = 0.098 kPa)]were higher than that at T0 [(6.3 ±0.7) cm H2O]and that in control group ( P < 0.05). Hb and Hct at T1 and T2 in AHH group were lower than that at T0 and that in control group (P< 0.05). Plt and FIB at T1 and T2 in AHH group decreased significantly than that at T0(P<0.05), while there were no statistical difference between two groups(P> 0.05). PT, TT and APTT in both groups had no statistical difference within or between groups (P > 0.05). Conclusion AHH with hydroxyethly starch 130/0.4 has no significant effect on function of blood clotting in patients undergoing spinal operation.

It is one of the common problems faced by many medical schools how to carry out the hidden curriculum in humanities education of medical education.This article approaches the composition and connotation of the curriculum in order to improve the relevance and effectiveness of the humanities education of medical education

Objective:To determine the diagnostic performance of dynamic contrast-enhanced MRI (DCE-MRI) in patients with recurrent or residual prostate cancer after radical prostatectomy. Methods:Studies were systematically searched in the PubMed, EMBASE, Cochrane library, SCI, CBM, CNKI, VIP, WanFang, and other databases. Additional studies were manually searched using the references of the retrieved articles. The retrieved deadline time was June 6th, 2013. Studies were eligible for inclusion based on the inclusion and exclusion criteria, and the qualities of the studies were reviewed based on the QUADAS criteria. The Meta Disc 1.4 software was used for meta-analysis, and a summary receiver operating characteristic (SROC) curve was constructed. The patient-based pooled weighted estimates of the sensitivity, specificity, diagnostic odds radio, and 95%confidence interval were calculated. Results:Among the 118 eligible studies, 7 articles (12 studies) were included in the meta-analysis. The pooled weighted estimates of the sensitivity, specificity, and the area under the curve were 0.88 (95%confidence interval:0.84 and 0.91), 0.87 (95%confidence interval:0.81 and 0.92), and 0.939 1, respectively. The pooled diagnostic radio (dOR) was 50.4 (95%confidence interval:26.0 and 97.6) and Q*was 0.876 4. Conclusion:DCE-MRI has high sensitivity and specificity in the evaluation of locally recurrent or residual prostate cancer after radical prostatectomy, in which the diagnostic efficiency of DCE-MRI combined with magnetic resonance spectroscopy is much better.

In recent years, a large number of studies have found that central lymph node metastasis of the papillary thyroid microcarcinoma (PTMC) is significantly related with some risk factors.These high risk factors include age below 45 years old, BRAF gene mutation, vascular epithelial growth factor-C high expression, tumor diameter≥5 mm, multifocal and bilateral tumor, thyroid capsule invasion and so on.These high risk factors involve in disease process, and interact with each other.The studies of the high risk factors can help us to predict the risk of central lymph node metastasis in the patients with PTMC, thus to effectively guide the patients' treatment.