Adaptation, Psychological ; Adolescent ; Adult ; Coal Mining ; Humans ; Male ; Mental Health ; Middle Aged ; Psychology, Industrial ; Young Adult

Objective To study the applicability of hysteroseopy for abnormal uterine bleeding of women and to analyze the etiology.Methods 679 cases of women with abnormal uterine bleeding were examined by hysteroscopy,whose situations were showed first under bimanual or trimanual examinations,then trams abdominal or traps vaginal B-ultrasonograpy.Results The postive rate of uterie abnormality was 98.6% detected by hysteroscopy and biopsy.Endometrial hyperplasia and endometrialpolyp were the main cause of abnormal uterine bleeding,which occupied 56.7%,then were myoma and endometritis.Women of child-bearing age were the largdy group that in volved.Conclusion Hysteorscopic examination was useful for abnormality uterine bleeding of women.We can underatand the relationship with abnormal uterine bleeding and intra-uterine disease,and the distribution of the different diseases in different age to enhance accurate diagnosis.

Objective To elucidate the effects of endothelin-1(ET-1) on expression of extra-regulated kinase 1(ERK 1), cell cycle and changes of Ca 2+ in activated hepatic stellate cells (HSCs). Methods Effects of different concentra tions of ET-1 on ERK 1 expression were determined by Western blotting. Effects of ET- 1 on cell cycle were observed by flow cytometry(FCM) analysis. Effects of ET-1 on changes of calcium concentration and cell area were observed by laser scanning confocal microscopy (LSCM). Results ET-1 could inhibit the expression of ERK 1 i n a dose-dependent manner, compared with the control, the expression of ERK 1 in E T-1 highest concentration group decreased 10.91%?3.36% (P

Objective: To investigate the effects of five kinds of flavonoids (calycosin, formononetin, ononin, isoliquiritigenin, and medicarpin) from Hedysari Radix on promoting osteogenic differentiation of rat bone marrow stromal cells (rBMSCs) and rat calvarial osteoblasts (ROBs). Methods: rBMSCs were isolated according to plastic adherence. ROBs were isolated by enzyme digestion method. The proliferation of rBMSCs and ROBs were detected by MTT assay. ALP activity and calcium content of rBMSCs and ROBs cells were detected by alkaline phosphatase kit and calcium kit. Mineralized nodule formation was detected by alizarin red staining. Results: The five components could promote proliferation, increase ALP activity, increase calcium content, and increase the area and number of calcified nodules of rBMSCs and ROBs (P < 0.05). Among them, calycosin had the best effect on promoting the osteogenic differentiation of rBMSCs, and medicarpin promoted the osteogenic differentiation of ROBs with the best effect, followed by calycosin. Conclusion: Five flavonoids promoted the improvement of osteogenic function, while calycosin has better osteogenic activity on rBMSCs and ROBs and can be used as an excellent osteoinductive factor.

[Objective]To investigate the effects of YIGUTANG contaning serum on osteoblasts proliferation which was from the skull of newborn SD rat in vitro.[Methods] The osteoblast from newborn SD rats’skull adopted, take the method of collagenase-pancreatic enzyme digestion,then respectively culture these osteoblasts with different concentration of the YIGUTANG drug containing serum fluid together.[Results]YIGUTANG drug containing serum could stimulate the proliferation of osteoblast, and the high,the middle and the low concentrations groups contrasted with the control group, all could promote the proliferation of cell .The drug containing serum groups had insignificant difference from the blank control group(P

ObjectiveTo compare the influence of laparoscopic and abdominal hysterectomy on cellular immune function. Methods There were 28 patientsreceived laparoscopic hysterectomy (laparoscopic group) and 28 patients treated with abdominal hysterectomy (abdominal group).The surgical effects and the changes of preoperative and postoperative T lymphocyte subsets of two groups were compared.ResultsThe intraoperative blood loss,anal exhaust time,hospital stay and postoperative morbidity of the laparoscopic group were significantly better than those of the laparotomy group[ (84.7 ± 21.7) ml vs.( 108.0 ± 23.8) ml,(19.3 ±4.1) h vs.(23.8 ±3.8) h,(5.12 ± 1.14) d vs.(7.81 ±2.27) d,7.1％ (2/28) vs.17.9％(5/28),P ＜ 0.05 ].CD4 and CD8 in the 1st day after operation of two groups were significantly lower than that before surgery respectively [ laparoscopic group:(38.41 ± 5.52)％ vs.( 40.72 ± 6.46)％,(24.41 ± 3.78 )％ vs.(26.33 ± 4.17)％ ;abdominal group:(38.41 ± 4.97)％ vs.(40.13 ± 6.12)％,(24.41 ±6.32)％ vs.(26.25 ±4.56)％,P ＜ 0.05 ]; but CD4 and CD8 in the 3rd day after operation in the laparoscopic group[ (40.15 ±6.29)％,(27.23 ± 5.12)％] almost returned to normal level before surgery,while CD4 and CD8 in the 3rd day after operation in the abdominal group [ (36.15 ± 5.12)％,(23.15 ± 4.87 )％] still had significant differences compared with that before surgery(P ＜ 0.05).ConclusionsLaparoscopic hysterectomy with less blood loss,rapid postoperative recovery,fewer complications and less impact on cellular immune function,is superior to abdominal hysterectomy.Therefore,it deserves promotion and wide application.

Objective To analyze the correlation of gamma-glutamyl transpeptidase (GGT)level with alpha-fetoprotein (AFP)level and to re-evaluate the diagnostic value of GGT for hepatocellular carcinoma (HCC).Methods Four hundred and seventy-two patients with HCC or liver cirrhosis,who were hospitalized in China-Japan Friendship Hospital from January 2003 to June 2009,were included in the study.The correlation between GGT and AFP was analyzed by Spearman nonparametric test.The cut-off values for the two parameters were determined based on their receiver operating characteristics (ROC)curves,areas under the ROC curve (AUCs),sensitivity,and specifici-ty,and the diagnostic values were presented using their sensitivity,specificity,and correct index.Statistical analysis was performed using SPSS 17.0.Normally distributed continuous data were analyzed by independent-samples t test,while non-normally distributed continuous data were analyzed by Mann -Whitney U test.Categorical data were analyzed by Pearson chi -square test,continuity-corrected chi -square test,or Fisher’s exact test.Results Among 472 patients,224 were diagnosed with HCC,and 248 with liver cirrhosis.Compared with cirrhotic patients,HCC patients had a significantly higher GGT level (113 (58-254)U/L vs 38 (22-72)U/L,Z=-11.037,P<0.001)and a significantly higher AFP level (429.5 (15.7-1210.0)ng/ml vs 5.7 (3.4-18.2)ng/ml,Z=-10.157,P<0.001).A significant correlation was found between GGT and AFP (r=0.449,P<0.001).The AUC was 0.784 for GGT and 0.788 for AFP.The cut-off value was 60 U/L for GGT and 20 ng/ml for AFP.The sensitivity was 74.1%for GGT,71.8%for AFP,and 90.7%for a combina-tion of the two parameters,the specificity was 70.2%,77.6%,and 58.7%,respectively,and the correct index was 0.443,0.494,and 0.494,respectively.Conclusion GGT may be regarded as one biomarker for HCC,and its level is significantly correlated with AFP level. The diagnostic value of AFP may not be improved when used in combination with GGT.

Objective To evaluate the effects of conventional mechanical ventilation (CMV) and mechanical ventilation with ideal PEEP and permissive hypercapnia (PHY + PEEPi) on expression of ?-defensin-2 (HBD-2) gene and protein in ventilator-associated pneumonia (VAP). Methods Forty-eight male SD rats weighing 280-320 g were randomly divided into 2 groups: (1) CMV group (VT = 12 ml? kg-1 , RR = 70bpm, FiO2 = 1.0) (n = 24) and (2) PHY + PEEPi group (VT =6 ml?kg-1 , RR = 90bpm, PEEP = 0.2 kPa, FiO2 = 1,0) (n = 24) . The animals were anesthetized with intraperitoneal 20% urethane 1 ml?100 g-1 , tracheotomized and mechanically ventilated. After being ventilated for 24 h P. aeruginosa (3 ? 108 CFU/ml) 0.2 ml was introduced into trachea to induce pulmonary infection. Three animals were sacrificed at following intervals: before and 1.5 h, 3h,6h,12h,3d and 5 d after introduction of P. aeruginosa. Lung tissaes were obtained from middle and lower lobes of left lung for microscopic examination and determination of expression of mRNA of HBD-2 by RT-PCR and HBD-2 protein level by Western blot analysis. Right lung was lavaged and broncho-alveolar lavage fluid (BALF) was collected for bacteriological examination. Results There were significantly more severe pathological changes in the lung in CMV group as compared with PHY + PEEPi group. In CMV group the levels of up-regulation of HBD-2 mRNA and protein expression were significantly lower after 3h than those in PHY + PEEPi group. The positive rate of blood and BALF bacterial culture was also higher in CMV group. The survival rate of PHY + PEEPi group was 76% , significantly higher than that of CMV group (40% ) ( P

Objective To determine and compare the minimum local analgesic concentration(MLAC) of ropivacaine and bupivacaine in continuous block of axillary brachial plexus for postoperative active mobilization of flexor tendon. Methods Seventy ASA Ⅰ or Ⅱ patients undergoing relaxation of flexor tendon were randomly allocated into 2 groups (n = 35 each): ropivacaine group (group R) and bupivacaine group (group B). A catheter was inserted into axillary sheath using B. Braun stimulating intraducer, 24-48 h after operation. 30 min before functional exercise 20 ml of ropivacaine or bupivacaine was injected through catheter. The minimum local analgesic concentration of both local anesthetics was determined by up-and-down sequential test. The first concentration of both local anesthetics was 0.1%. The next concentration of local anesthetic was determined by response of the previous patient. The analgesia efficacy was assessed using 100 mm VAS score (0 = no pain, 100 mm = worst possible pain) . Increase/decrease in VAS by 10 mm was defined as effective. An effective result resulted in increase/decrease by 0.01% in the next patient. The motor block was also recorded.Results The two groups were comparable with regard to age, sex, body weight and baseline VAS score. The MLAC of ropivacaine was 0.133% [95% confidence interval (CI), 0.128%-0.138%] and MLAC of bupivacaine was0.121% (95%CI, 0.116%-0.126%). The analgesic potency of ropivacaine was 10% less than that of bupivacaine. The potency ratio of ropivacaine to bupivacaine was 0.91. The degree of motor block is higher with bupivacaine than with ropivacaine. Conclusion The results of our study show that the analgesic potency of ropivacaine is 10% less than that of bupivacaine. The degree of motor block is higher with bupivacaine than with ropivacaine.

Objective:To explore whether tumor-inducing agent 12-O-tetradecanoyl-phorbol-13-acetate (TPA) and bromodeoxyuridine (BrdU) affect CD133 expression in nasopharyngeal carcinoma (NPC) 5-8F cells. Methods:NPC cell line 5-8F was treated with single TPA, single BrdU, or TPA plus BrdU, respectively. CD133 mRNA and protein expression levels were detected by real-time fluorogentic quantitative PCR and Western blotting, respectively. Flow cytometry was used to separate CD133-positive cells and determine their levels. Boyden chamber test was used to measure the invasion capability of the cells. Results:Compared with untreated group, CD133 mRNA levels were increased in single BrdU group and BrdU plus TPA group (P=0.037 and 0.003, respectively), and decreased in single TPA group. Western blotting indicated that the expressions of CD133 protein was increased in all the three treated groups, and FCM showed that the quantity of CD133-positive cells also increased. The invasion capability was enhanced, especially in BrdU plus TPA group. Conclusion:Both TPA and BrdU increased CD133 expression in NPC.The effects of TPA and BrdU are synergestic.