In the past few years, people have a new understanding of the mechanism of Epigenetic disease. Epigenetic modification processes involved in human disease include DNA methylation, histone acetylation and methylation, RNA-associated silencing.Epigenetic modification related with cell growth, differentiation, apoptosis, transformation and tumor progression-related gene transcription. There has been a long time that the gene mutations involved in tumor formation. In recent years.more and more evidences that genetic and epigenetic modifications leading to the formation of tumors. Epigenetic modifications has also play a very important role in the formation of tumors. This article discussed the DNA methylation.histone post-translational modification anomalies and small interference RNA impact on the formation of leukemia. It provides new prospects for the treatment of leukemia.

Manganese-enhanced MRI (MEMRI) optic nerve tracing in vivo is a technology which can reflect the axonal transport status and visual pathway connection by utilizing divalent manganese ion's transportability in axons and its characteristics of paramagnetism,and such potential optic nerve tract-tracing technique has been widely used in ophthalmologic research.Mn2+ enters the neurons through the voltage-gating Ca2+ channel and is transported by microtubule system which is not depended on cellular electric activity.However,the trans-synaptic transport of Mn2+ relies on membrane potential.Taking advantages of MEMRI,optic nerve injury-related research could be implemented.Mn2+ is an intracellular contrast agent with metal toxicity,so low dose,fractionated,targeted delivery of Mn2+ is recommended to alleviate the adverse effects of Mn2+.Herein,the mechanism of divalent manganese ion optic nerve tracing,application of MEMRI in ophthalmologic research field,drug delivery approach of divalent Mn2+ and its toxicity were summarized.

Objective To study the influence of defection of Fragile X mental retardation-1 gene (FMR1) on cyclic adenosine monophosphate (cAMP) and to discuss its mechanism. Methods FMR1 gene of peripheral blood mononuclear cell was silenced in vitro by sodium nitrointroprusside. The effect of gene-silencing was detected using reverse transcript polymerase chain reaction (RT-PCR). The specific activity of adenylate cyclase and phosphodiesterase was showed by the activity ratio of yield or consumption of cAMP during a unit time. Spectrophotometry was used to measure the two key enzymes (adenylate cyclase and phosphodiesterase), as to determining the level of intracellular cyclic adenosine monophosphate in the process of cAMP metabolism. Results FMR1 gene was fully silenced by sodium nitrointroprusside at 12th, 24th and 48th hour separately, re-expressed at 72th hour. If the cultivated fluild was replaced with new sodium nitrointroprusside at 48th hour, FMR1 gene would be silenced continuously. The intracellular cAMP level in the gene silenced group was lower, and significant depression of adenylate cyclase specific activity was found in the FMR1 gene silenced group (P=0.000). No significant difference was found on phosphodiesterase specific activity (P=0.983). Conclusions The results suggest that the yield of cAMP could be influenced by defection of FMR1. The depression of adenylate cyclase activity might be one of the causes of the decrease of intracellular cAMP production.

Objective To investigate the effects of inhibition of nuclear factor-kappa B (NF-?B) by pyrrolidine dithiocarbamate (PDTC) on tissue pro- and anti-inflammatory cytokine expression in rats with postburn Staphylococcus aureus sepsis and its potential regulating mechanism. Methods Thirty-four male Wistar rats were randomly divided into normal control group (n=6), scald control group (n=6), postburn sepsis group (n=12),and PDTC treatment group (n=10). Tissue samples from the liver, kidneys and lungs were collected to determine tumor necrosis factor-? (TNF-?) and interleukin-10 (IL-10) mRNA expression as well as their protein levels. Results Both TNF-? mRNA and protein expressions in the liver, kidneys and lungs of postburn septic animals were up-regulated rapidly at 0.5-2 hours (P

Bacterial cellulose,as a new material,due to its unique nano-fiber mesh structure with good purity,physical and chemical properties such as mechanical strength and water holding capacity,biocompatibility and adaptability,has been widely used in medicine,food,paper,textile,acoustical materials and other industries.In medical field,especially,it has been rapidly developed in recent years.This review discusses the current bacterial cellulose and its performance optimization products which relates to the medical applications.

Objective: To explore auxiliary diagnostic value of fragmented QRS complex on ECG for acute myocardial infarction (AMI).Methods: Clinical data of 102 AMI patients (AMI group) and 132 CHD patients (CHD) group, who were treated in our hospital from Apr 2013 to Apr 2014, were retrospectively studied, another 120 healthy subjects undergoing physical examination in our hospital during the same period were regarded as normal control group.AMI was diagnosed by myocardial perfusion tomography, all subjects received ECG examination, and incidence rates of fragmented QRS complex and pathological Q wave at different sites were recorded.Sensitivity, specificity and accuracy of fragmented QRS complex and pathological Q wave diagnosing AMI were compared.Results: Incidence rate of fragmented QRS complex was highest on inferior wall in AMI group, and that of pathological Q wave was highest on anterior wall in AMI group.Except fragmented QRS complex in sidewall, compared with normal control group, there were significant rise in incidence rates of fragmented QRS complex on anterior wall (0.8% vs.22.5%, 13.6%) and inferior wall (6.7% vs.26.5%, 17.4%);pathological Q wave on anterior wall (0 vs.41.2%, 29.5%), inferior wall (0.8% vs.40.2%, 35.6%) and side wall (0 vs.2%, 2.3%) in AMI group and CHD group, P<0.01 all.Compared with pathological Q wave, there was significant rise in diagnosing sensitivity for AMI (40.00% vs.89.47%), for CHD (41.94% vs.91.50%) of fragmented QRS complex (P<0.01 both), but there were no significant difference in specificity and accuracy between them (P>0.05 all).Conclusion: Fragmented QRS complex can be used as an important auxiliary index diagnosing acute myocardial infarction.When combined with pathological Q wave, it can further improve diagnostic value of ECG for acute myocardial infarction.

Aim To investigate the effect of Sonic Hedgehog on normal hearts and hypoxic-ischemic myo-cardial cells. Methods A method for left anterior de-scending artery ( LAD ) ligation was employed to con-struct the myocardial infarction model, and ultrasonic cardiogram was used for identification. Western blot and immunofluorescence staining were used to detect expressions of Shh, Ptch-1, Smo and Gli-1 in H9C2 cells and H2 O2-induced H9C2 cells, and that in 12 ca-ses of myocardial infarction tissues and 9 cases of nor-mal myocardium, respectively. Agonist and antagonist of Shh pathway were adminstered in the hypoxic-ische-mic myocardial H2 O2-induced H9C2 cell model, and once again expressions and strength of Shh, Ptch-1, Smo, Gli-1 were detected. Results Shh and Gli-1 were not expressed in normal hearts, but expressed in hearts with myocardial infarction;Ptch-1 and Smo were expressed in both normal hearts and hearts with myo-cardial infarction. Under the action of agonist, expres-sions of Shh and Gli-1 increased in the hypoxic-ische-mic H9C2 cell model. Similarly, Shh and Gli-1 were not expressed in normal H9C2 cells, but in H2 O2-in-duced H9C2 cells, and Ptch-1 and Smo were expressed in both normal H9C2 and in H2 O2-induced H9C2 cells. Conclusion Shh signaling pathway can be acti-vated in the condition of ischemia and oxidative stress, and then it promotes the repairing of myocardial cell damage.

Objective To explore the effect of Kangfu antiphlogistic suppository combined with ceftriaxone on the treatment of chronic pelvic inflammation.MethodsFrom March 2015 to January 2017 in Deqing hospital of traditional Chinese medicine, 80 cases with chronic pelvic inflammatory as research object were divided into the observation group and the control group, 40 cases in each group.The control group were given Ceftriaxone, at this basis, the observation group were given Kangfu antiphlogistic suppository, The effect and the adverse reaction in the two groups were compared.ResultsThe effect was 92.5% in the observation group, which was higher than that in the control group 77.5%, the difference was statistically significant,P<0.05.There was no adverse reaction in the 2 groups.ConclusionThe effect is exactly that Kangfu antiphlogistic suppository combined with ceftriaxone sodium on the treatment of chronic pelvic inflammation, which is worthy of clinical use.

Objective To develop the Chinese version of Patient Learning Needs Scale(PLNS), and evaluate the feasibility, validity and reliability of the developed scale. Methods The standard procedure of cross-culture adaptation was used to develop the Chinese version PLNS. A total of 385 adults hospitalized with a medical or surgical illness completed the Chinese version PLNS. The feasibil-ity and psychometric properties of the scale such as, internal consistency, split-half reliability, content validity and construct validity were evaluated. Results Questionnaire recovery rate of the investiga-tion was 98.5%,the completion rate was 97.7%. Average time of completing was (13.75±4.36) min. The overall Cronbaeh's α coefficient was 0.95 and the value of a in 5 subscales of PLNS ranged from 0.75 to 0.86. The overall split-half reliability coefficient was 0.95 and the value of split-half in 5 subscales of PLNS ranged from 0.802 to 0.876. The CVI for content validity was 0.86. The construct validity was con-finned by factor analysis with 55,08% variance was explained by 9 factors. Conclusions An accept-able psychometric property of the Chinese version of PLNS was indicated. PLNS could be used as positive aspects measurement of general patients'perceptions of learning needs while discharging.It might be help-ful for care and further clinical study.

OBJECTIVE:To explore a reimbursement mechanism for adverse drug reactions(ADR)suitable to the condition of China.METHODS:Based on the condition in China,we evaluated the feasibility of applying insurance mechanism into ADR reimbursement from aspects of the development of insurance industry and pharmaceutical industry as well as the characteristics of ADR etc.Meanwhile we put forward some suggestions on the construction and operation of the ADR liability insurance.RESULTS & CONCLUSIONS:ADR liability insurance can help guarantee patients' legitimate rights of life health,settle medical disputes,facilitate the development of pharmaceutical industry and promote ADR reporting and management work.To carry out ADR liability insurance,issues like insurance rate,national policy support and evolving of ADR monitor network should be taken into consideration.