Manganese-enhanced MRI (MEMRI) optic nerve tracing in vivo is a technology which can reflect the axonal transport status and visual pathway connection by utilizing divalent manganese ion's transportability in axons and its characteristics of paramagnetism,and such potential optic nerve tract-tracing technique has been widely used in ophthalmologic research.Mn2+ enters the neurons through the voltage-gating Ca2+ channel and is transported by microtubule system which is not depended on cellular electric activity.However,the trans-synaptic transport of Mn2+ relies on membrane potential.Taking advantages of MEMRI,optic nerve injury-related research could be implemented.Mn2+ is an intracellular contrast agent with metal toxicity,so low dose,fractionated,targeted delivery of Mn2+ is recommended to alleviate the adverse effects of Mn2+.Herein,the mechanism of divalent manganese ion optic nerve tracing,application of MEMRI in ophthalmologic research field,drug delivery approach of divalent Mn2+ and its toxicity were summarized.

In the past few years, people have a new understanding of the mechanism of Epigenetic disease. Epigenetic modification processes involved in human disease include DNA methylation, histone acetylation and methylation, RNA-associated silencing.Epigenetic modification related with cell growth, differentiation, apoptosis, transformation and tumor progression-related gene transcription. There has been a long time that the gene mutations involved in tumor formation. In recent years.more and more evidences that genetic and epigenetic modifications leading to the formation of tumors. Epigenetic modifications has also play a very important role in the formation of tumors. This article discussed the DNA methylation.histone post-translational modification anomalies and small interference RNA impact on the formation of leukemia. It provides new prospects for the treatment of leukemia.

Objective To study the influence of defection of Fragile X mental retardation-1 gene (FMR1) on cyclic adenosine monophosphate (cAMP) and to discuss its mechanism. Methods FMR1 gene of peripheral blood mononuclear cell was silenced in vitro by sodium nitrointroprusside. The effect of gene-silencing was detected using reverse transcript polymerase chain reaction (RT-PCR). The specific activity of adenylate cyclase and phosphodiesterase was showed by the activity ratio of yield or consumption of cAMP during a unit time. Spectrophotometry was used to measure the two key enzymes (adenylate cyclase and phosphodiesterase), as to determining the level of intracellular cyclic adenosine monophosphate in the process of cAMP metabolism. Results FMR1 gene was fully silenced by sodium nitrointroprusside at 12th, 24th and 48th hour separately, re-expressed at 72th hour. If the cultivated fluild was replaced with new sodium nitrointroprusside at 48th hour, FMR1 gene would be silenced continuously. The intracellular cAMP level in the gene silenced group was lower, and significant depression of adenylate cyclase specific activity was found in the FMR1 gene silenced group (P=0.000). No significant difference was found on phosphodiesterase specific activity (P=0.983). Conclusions The results suggest that the yield of cAMP could be influenced by defection of FMR1. The depression of adenylate cyclase activity might be one of the causes of the decrease of intracellular cAMP production.

Objective To investigate the effects of inhibition of nuclear factor-kappa B (NF-?B) by pyrrolidine dithiocarbamate (PDTC) on tissue pro- and anti-inflammatory cytokine expression in rats with postburn Staphylococcus aureus sepsis and its potential regulating mechanism. Methods Thirty-four male Wistar rats were randomly divided into normal control group (n=6), scald control group (n=6), postburn sepsis group (n=12),and PDTC treatment group (n=10). Tissue samples from the liver, kidneys and lungs were collected to determine tumor necrosis factor-? (TNF-?) and interleukin-10 (IL-10) mRNA expression as well as their protein levels. Results Both TNF-? mRNA and protein expressions in the liver, kidneys and lungs of postburn septic animals were up-regulated rapidly at 0.5-2 hours (P

Bacterial cellulose,as a new material,due to its unique nano-fiber mesh structure with good purity,physical and chemical properties such as mechanical strength and water holding capacity,biocompatibility and adaptability,has been widely used in medicine,food,paper,textile,acoustical materials and other industries.In medical field,especially,it has been rapidly developed in recent years.This review discusses the current bacterial cellulose and its performance optimization products which relates to the medical applications.

Objective To explore the diagnostic significance of the uric acid in preeclampsia.Methods The samples were chosen between January 2008 and August 2015 in our hospital maternity clinic,at 24 to 28 weeks of gestation,singletons,1 357 cases of pregnant women,136 cases for the development of preeclampsia,and 1 221 cases of normal pregnancy.the clinical data of 1 357 cases were retrospectively analyzed.Results (1) The maternal age,birth gestational age,body mass index,systolic blood pressure,diastolic blood pressure,urine protein,cholesterol,creatinine,uric acid,and uric acid increased value of the control group of preeclampsia were significandy higher than the normal group (P ＜ 0.05).The incidence of preeclampsia,cesarean delivery rate,fetal growth restriction,small gestational age,and newborn asphyxia rate of high uric acid hematic disease group were significandy higher than the normal group (P ＜ 0.05).(2) Logistic regression analysis showed that systolic blood pressure,urinary protein and uric acid were the predictors,and uric acid was the best predictor.(3) By using receiver operating characteristic (ROC) curve,the growth of uric acid was 112 μmol/L,and the sensitivity was 85.2％,the specificity was 90.1％.Conclusions Uric acid level can be used as an important indicator to monitor the pathophysiology of preeclampsia,and uric acid value can be accurately predicted.

mia and hypertension are the major risk factors for occurrence of diabetic retinopathy.High UA may be also associated with occurrence of diabetic retinopathy.

SWOT method was used to analyze the internal strengths,weaknesses,external opportunities and threats of Nanjing General Hospital of Nanjing Military Command,PLA. Based on the results,four development policy were put forward:developing the sections prepared for war; inducing invigorative system in human resource management; enhancing the value of nosocomial brand; strengthening of the audit about the medical cost accounting.

Objective To investigate the effect of different levels of propofol anesthesia on 5-HT metabolism in brain. Methods Forty Japanese Long-ear rabbits of both sexes, aged 8 months-2yr, weighing 2-3 kg were used for study. Anesthesia was induced and maintained with propofol administered by a TCI system. Blood propofol concentration required for different depths of anesthesia, as indicated by BIS monitoring and loss of chewing reflex (light anesthesia) and loss of response to tail nipped (deep anesthesia), was determined by high-performance liquid chromatography(HPLC) in 10 rabbits. Thirty rabbits were randomly divided into 3 groups often animals in each group: (1) control group received no propofol; (2) light anesthesia group and (3) deep anesthesia group. Different depths of propofol anesthesia were maintained for 1 h. At the end of one hour propofol anesthesia the animals were decapitated. Brain was immediately removed and cerebral cortex, hippocampus and thalamus were separated on ice. Their contents of 5-HT and 5-hydroxyindole-3-acetic acid (5-HIAA) were measured by spectrophoto-fluorometer.Results 5-HT content of cerebral cortex was significantly increased after anesthesia. 5-HT content of hippocampus and 5-HIAA content of thalamus decreased significantly with increasing anesthetic depth. Conclusion Propofol improves 5-HT activity in cerebral cortex and thalamus and decreases 5-HT activity in hippocampus indicating that 5-HT metabolism may be involved in the mechanism of propofol anesthesia.

This paper analyzes the absence of humanities in the closed management of psychiatric ward,and proposes relevant countermeasures: strengthening absorbing knowledge in humanities,applying humanistic nursing care,promoting the cultural construction of humanities in psychiatric ward,and strengthening the mental health support for nurses in psychiatric ward.