Objective To report the clinical results of the treatment for recurrent immunologic disorders of cornea and conjunctiva by conjunctival resection with amniotic membrane transplantation(AMT). Design A retrospective clinical case series. Participants Five patients 5 eyes with recurrent immunologic disorders of cornea and conjunctiva were enrolled into this study, including 2 eyes of phlytenular keratoconjunctivitis with corneal lesion, 1 eye of nodular episcleritis, 2 eyes of corneoscleral interstitial keratitis. Methods Patients were treated with partial conjunctiva resection and AMT. After operation, steroids and cyclosporin eye-drops were given for 2-3 weeks, and patients were followed up for 6-12 months. Main Outcome Measures Complications and recurrent situations were observed. Results The corneal disease kept quiescent, and the blood vessel subsidized. There were no recurrences during the follow up period in 4 cases. Recurrent signs were noted in one patient at 12 months after surgery. But no recurrence of corneal lesion was noted although the conjunctivitis was severe. Conclusion Local conjunctiva resection combined with AMT is a useful method for recurrent cases of immunologic disorders of cornea and conjunctiva. (Ophthalmol CHN, 2006,15:177-179)

In recent years, a large number of studies have found that central lymph node metastasis of the papillary thyroid microcarcinoma (PTMC) is significantly related with some risk factors.These high risk factors include age below 45 years old, BRAF gene mutation, vascular epithelial growth factor-C high expression, tumor diameter≥5 mm, multifocal and bilateral tumor, thyroid capsule invasion and so on.These high risk factors involve in disease process, and interact with each other.The studies of the high risk factors can help us to predict the risk of central lymph node metastasis in the patients with PTMC, thus to effectively guide the patients' treatment.

Objective]To expound the essence and method of detoxification in the view of keeping internal balance ,based on Huangdi’s Internal Classic. [Method] From the present social understanding of toxin biased ,and on the basis of collecting and analyzing the materials concerning the record of concept and treatment of eliminating toxin in early medical classic ,eliminating toxin and keeping balance can be taken as a sort of health care idea. [Result] The toxin in body refers to the imbalance of Yin and Yang Qi and blood of human body ,it also may be the cause and new pathological products. This recognition corrects the understanding of contemporary society point ofDetoxification ,and guides people to follow the body's own adjustment mechanism,the function of organs and individual constitution to determine etiologic factors based on differentiation treatment. It will avoid and prevent producing the toxin in accordance withIn order to maintain body balance and adjust yin and yang of body through careful diagnosis said by Huangdi ’s Internal Classic. [Conclusion] That one specific treatment depending on one specific toxin reflects the principles of syndrome differentiation treatment and searching for the primary cause of disease in treatment by exploring Huangdi ’s Internal Classic. And it can make people understand more accurately and profoundly about detoxification and live healthier.

A reusable chronocoulometric adenosine triphosphate (ATP)-aptamer sensor was developed in this work.A short chain of DNA marked as cDNA containing complementary sequence was immobilized on gold electrode based on Au-S self-assembly.The ATP aptamer was hybridized with cDNA.The surface-confined DNA could bind with [Ru(NH3)63+ (RuHex) in the electrolyte via electrostatic interaction.Upon target ATP binding, the aptamer confined onto electrode surface was disassociated from the cDNA oligonucleotides into the solution.Such surface density change of DNA lead to the decrease of chronocoulometric signal for the RuHex which confined on the electrode surface.The chronocoulometric signals showed a linear relationship with logrithm of ATP concentration in the range of 1 nmol/L to 100 μmol/L, and the detection limit of this aptamer sensor could reach 0.5 nmol/L (S/N=3).This aptamer sensor could be regenerated 5 times by simple steps.With this aptamer sensor, the basal level of ATP in the brain cortex micorodialysate was determined to be 19.2±3.7 nmol/L (n=3).

Objective To know the masons of delaying to receive medical treatment among patients with breast cancer, and then reference to related health education. Methods 13 patients with breast cancer were selected in this research, interviewed them by phenomenological method to know their reasons of delaying to receive medical treatment, analized the results of interview by Clolaizzi method. Results The were five themes for patients with breast cancer about delaying to receive medical treatment, they were not understand breast cancer-related knowledge, shortage of method of self-examination, psychological factors, working pressure and family factors, social environment factors. Conclusions We should strengthen breast cancer-related health education when changed women's traditional ideas and ameliorated their family and social environment.

Objective To investigate the possibility of pulp regeneration of different irreversible pulpitis with absorbable gelatin sponge as the scaffold,and dental seed cells,induced molecules and MTA closed.Methods 150 patients with irreducible pulpitis,who were removed the crown infection pulp and retained the root living pulp,according to the visiting sequence,were randomly divided into two groups.The experimental group was given absorbable gelatin sponge as the scaffold and MTA closed.The control group was given calcium hydroxide coverage.The symptoms situation and pulp situation were observed.Results 6 months after surgery,the success rate of acute pulpitis and chronic closed pulpitis in the experimental group was 76.5％ and 47.0％,which were significantly higher than those of the control group (14.3 ％,8.8％) (P ＜ 0.01).In the experimental group,the success rate of chronic hyperplastic pulpitis and chronic ulcerative pulpitis was 33.0％ and 35.0％,which were higher than those of the control group(9.1％,6.0％),but the differences between the two groups were not statistically significant (P ＞ 0.05).Conclusion Pulp regeneration in vivo is possible with absorbable gelatin sponge as the scaffold and MTA,pulp regeneration of acute pulpitis and chronic closed pulpitis are more likely to induce.

Objective To investigate the protective effect of the extract of Vaccinium Bracteatum Thunb(VBT) leaves against light injury of retina.Methods 20 Newzealand rabbits were divided into 4 groups: the normal control group,modelⅠgroup,modelⅡ group,and model Ⅲ group.The normal control group ate and drank freely.modelⅠgroup ate and drank freely but took in 50g/(kg?d) fresh VBT leaves daily for 4 weeks.model Ⅱgroup ate and drank freely,but took in 50g/(kg?d) old VBT leaves daily for 4 weeks.model Ⅲ group ate freely,but took in 50mg/(kg?d) extract of VBT leaves(resolved in water) daily for 4 weeks.All rabbits were exposed to strong light,meanwhile, the retinas were analyzed by electroretinogram(ERG) every week.Finally,all the rabbits were killed and the content of MDA and SOD in the retina were detected.Results In the control group,the latency time of the B wave increased and the amplitude of the B wave shortened,while in the model Ⅰ,Ⅱ and Ⅲ groups,the changes of B wave just opposite.The content of MDA in the model Ⅰ,Ⅱ,and Ⅲ groups was lower than that in the control group(P

Background and purpose:Gene therapy is a novel approach for the treatment of the patients with breast cancer. One of the effective ways is to direct transgenic expression to specific tissues or tumors with the use of tissue-specific-promoters (TSP). hTERT (human telomerase reverse transcriptase) is highly expressed in many types of cancers including breast cancer. Thus, we hypothesized that the hTERT promoter targeting with gene therapy vectors could be exploited for breast cancer. In this study, we amplified hTERT gene promoter and cloned it into the reporter vector pEGFP and pGL3-Basic. Afterwards, the specific transcription of hTERT promoter in MCF7 cells was evaluated. Methods:hTERT gene minimal promoter was PCR amplified and cloned into the reporter plasmid pEGFP-1 and pGL3-Basic.The constructs pEGFP/TERT and pGL3/TERT were transfected into MCF7 breast cancer cells and HBL100 human epithelial cells, respectively.The expression of EGFP and luciferase were investigated, respectively..Results:pEGFP/TERT and pGL3 /TERT bearing hTERT gene promoter were constructed. The specific expression of EGFP was detected in MCF7 cells while little expression of EGFP was seen in HBL100 cells.In accordance with EGFP, luciferase driven by hTERT also showed specific and high activity in MCF7 cell (RLU/U: 33784), which is 15 times higher than in HBL100 (RLU/U: 2400).Conclusions:The high transcriptional activity of hTERT gene promoter in MCF7 cell indicates its potential utility as a novel candidate for transcriptional targeting of breast cancer.

Objective To observe the expression of NGF mRNA in rats brain with diabetes and the effects of hypoglycemia caused by oral antidiabetic drugs on NGF mRNA.Methods A total of 64 adult Wistar rats were randomly divided into control group(16)and diabetes model group(48).Using high fat and high sugar diet+intraperitoneal injection of streptozotocin to prepare type 2 diabetes model.The diabetes model group was randomly divided into non-medication group(16),oral medication group (16) and hypoglycemia group(16).The oral medication rats and hypoglycemia rats were given glibenclamide+metformin by intragastric administration once daily for 12 d,the dose of hypoglycemia rats was 5 times of treatment rats.The brain tissues of rats in each group were used respectively the RT-PCR method to detect NGF mRNA at 3,6,9 and 12 d.Results Compared with the control group,the expression of NGF mRNA in the brain tissues of the 3 groups of diabetic rats were decreased in different degrees and the non-medication group was the most obvious(P＜0.01).Compared with the non-medication group,the oral medication group was significantly increased(P＜0.01),the hypoglycemia group was further decreased(P＜ 0.01).Conclusion Oral hypoglycemic drugs can effectively enhance the expression of NGF mRNA in brain tissue of diabetic rats,but hypoglycemia caused by Oral antidiabetic drugs can make the expression of NGF mRNA further decreased.So hypoglycemia is not conducive to the repair of diabetic neuropathy.

-4.88) and relative insulin resistant group (IAI≤-4.88), and 42 healthy normal persons were selected as control. Results The frequency of Trp 64Arg mutation of ? 3 AR gene was higher in the resistant group than that in the sensitive group (28.0% vs 15.7%, P