Pseudomonas fluorescens is one of the most common contaminative bacteria of the stored blood. Ps. fluorescens No. 10-36 was cultured at 4℃, 22℃ and 37℃ for studying its growth phase and logarithmic growth phase. The results showed that this strain grew slowly at 37℃ with a generative rate of 900 minutes, but had no difference between the lag phase and the logarithmic growth phase. At 22℃ the lag phase were 5 hours, and the logarithmic growth phase were 29minutes, while at 4℃, the lag phase was 30 hours and the logarithmic growth phase was 300 minutes.

Objective To analyze the characteristic of articles, authors and citations in Journal of Clincal Neurology from 2008 to 2015.Methods The data about articles, authors and citations in Journal of Clincal Neurology from 2008 to 2015 were analyzed by bibliometrics method.Results There were 1623 papers published in Journal of Clincal Neurology during the 2008 to 2015 years.The average number of articles per issue was 33.8.The average number of article per paper was 0.424.The papers with funded projects were 432 (26.6%).The first authors were mainly come from Jiangsu, Shandong, Beijing, Shanghai and Hebei, etc.The average cooperation degree was 4.17, and the average co-author rate was 95.81%.The average rate of the papers with citation was 93.96%, and the total number of citations was 18708.The citations were mainly come from foreign language (74.3%) and journals (97.7%).The average Price index was 37.72%.Conclusion Journal of Clincal Neurology is an academic journal of high quality, with strong influence in field of clinical neurology.

This study was aimed to analyze the influence of specific clinical effect of Dioscorea opposita Thunb. by dosage in order to summarize its rules. Recordings on pills containing D. opposita Thunb. from the classic prescrip-tion of the Formula Dictionary of Traditional Chinese Medicine were collected. Dosage of D. opposita Thunb. was used as the cardinal line. EXcellwas used to conduct schematic analysis. The results showed that small amount of D. opposita Thunb. had the effect of reinforcing the spleen. The effect of tonifying the lung had the dosage boundary line of 10 g. The small dosage had weak effect and large dosage had strong effect. The effect of tonifying the kidney was often the middle dosage. When the dose was larger or smaller, the effect was not obvious. The effect of consoli-dating the kidney was obvious when using larger or smaller dosage. It was concluded that the dosage of D. opposita Thunb. had relatively big influence on its effect, which provided reference of more value for the clinical practice and health cultivation.

Objective To explore the relationship between adaptive skills and intelligence in young children. Method 30 young children aged from 3 to 6 years were concurrently administered the Adaptive Skill Rating Scale for Young Children(ASRSYC) and China- Wechsler Young Chilren Scale of Intelligence(C -WYCSI). Result The correlation coefficients between the total score of ASRSYC and the verbal scale score , performance scale score and full scale score of C - WYCSI were 0. 85, 0.70and 0. 81, respectively. The corrdlations between the total score of ASRSYC and the subtest scores of C - WYCSI ranged from 0. 51 to 0. 75 . Conclusion Adaptive skills and intelligenoe in young children relate each other and develop jointly.

The afferent connections to the nucleus amygdaloideus centralis and nucleusamygdaloideus medialis in 42 albino rats were examined by means of retrogradetransport method of horseradish peroxidase(HRP).The microinjection syringe needle or microelectrophoretic pipette was insertedstereotaxically from vertical or oblipue approaches.The injections of HRP weregiven in the central and medial nuclei of the amygdaloid complex.The results were as follows:Following the injections of HRP into the nucleus amygdaloideus centralis,label-ed cells were always found in prepiriform cortex,periamygdaloid cortex,entorhinalcortex,ventral part the caudoputamen and globus pallidus,nucleus lateralis hy-pothalami,nucleus parafascicularis,corporis geniculati medialis,nucleus raphe dosalis,nucleus parabrachialis,locus coeruleus and zona reticulate substantia nigra.While HRP was injected into the nucleus amygdaloideus medialis,the labeledcells were always found in bed nucleus of stria terminalis,nucleus ventromedialis(hypothalami)and substantia nigra pars compacta.As the injections were given from lateral oblique approaches,labeled cells werenot found in the neo-cortex.There were no labeled cells demonstrated in the nuc-leus solitarius.In the above-mentioned areas nucleus ventromedialis(hypothalami),nucleusparabrachialis and locus coeruleus were often labeled bilaterally while others,ipsila-terally.

This article reports preliminarily the effects on the antibacterial activity & cytotoxicity of the derivatives of two diterpenoid components, Amethystoidin A & Rabdosia macrophylla C(i.e.Orido-nin ) which were isolated from the medicinal plants of Rabdosia ( Bl. ) Hassk. The experiment demonstrated that diacetate derivative of Amethystoidin A ( n ) could significantly enhance the antibacterial effect being 2 - 3 times more potent than that of Amethystoidin A. Hydrolytic derivative of Rabdosia macrophylla C (V) had a significant increase in the cytotoxicity on heptoma cell (QGY-7703 ) in vitro.

OCT angiography (OCTA) is a non-invasive imaging technique for detecting blood flow information of the retina and choroid.Dye injection is not needed with OCTA,which is different from fundus fluorescein angiography (FFA) and indocyanine green angiography (ICGA).OCTA is able to observe blood flow in different retinal and choroidal segmentation slab.This revolutionary breakthrough in OCTA algorithm provides more and more accurate blood flow informations in the diagnosis of ocular vessel diseases and the study on pathogenesis of some vessel-related eye diseases.However,like other biometric technology,OCTA has its limitations and shortcomings,for example,OCTA presents a smaller observational area than FFA and ICGA,and some factors affect the imaging quality and cause misdiagnosis during the examination and reading image.Fully understanding the principle of OCTA and its image features are helpful for eye doctors to better interpret the blood flow changes of retinal diseases,choroidal diseases,glaucoma and neuro-ophthalmic diseases.Ophthalmologists should correctly apply this imaging tool for a better monitoring and following up of these diseases.

Objective To evaluate the clinical effect of levofloxacin combined with Jingangteng particle in the treatment of patients with chronic pelvic inflammatory disease.Methods According to the random number table, sixty -four patients with chronic pelvic inflammatory disease were divided into control group(n =32)which were treated with levofloxacin and treatment group(n =32)which were treated with levofloxacin and Jingangteng particle for three weeks.The clinical effect,symptoms and signs were evaluated and the adverse reactions between the two groups were observed as well as serum C reactive protein(CRP)were measured before and after treatment.Results The score of symptoms and signs of the treatment group were significantly lower than the control group,the difference was statistically significant [(2.61 ±2.09 )points vs.(5.32 ±3.28)points,t =3.941,P =0.000;(1.47 ± 1.02)points vs.(2.56 ±2.04)points,t =2.703,P =0.008].The level of serum CRP of the control group was (5.46 ± 1.93)mg/L,which was statistically higher than (3.76 ±1.06)mg/L of the treatment group after treatment,the differ-ence was statistically significant (t =4.367,P =0.000).The total effective rate of the treatment group was 96.9%, which of the control group was 78.1%,the difference between the two groups were statistically significant(χ2 =5.142,P =0.023).There was no significant adverse reactions during the treatment.Conclusion Levofloxacin com-bined with Jingangteng particle can obviously improve the symptoms and signs of chronic pelvic inflammatory disease, they have good safety and are worthy of clinical popularization and application.

This study was aimed to determinate the content of quercetin from different parts (such as flowers, fruits, stems, leaves and branches) of Penthorum chinense Pursh. from Sichuan province so as to offer reference for the comprehensivedeve lopment and utilization of P. chinense Pursh. The quantitative determination of quercetin from five groups of different parts of P. chinense Pursh. were carried out and put into comparison by the reversed-phase high performance liquid chromatography (RP-HPLC). The results showed that there were larger differences of content among different parts. The highest content of the medical parts were flowers and fruits, which was followed by the leaves, stems and branches. It was concluded that different parts (such as flowers, fruits, stems, leaves and branches) of P. chinense Pursh. from Sichuan province should be divided in the clinical and productive practice, which was to supply the scientific basis for enhancing the curative effect and reasonable exploitation and utilization of the resource of P. chinense Pursh.

ObjectivesTo explore the impact of high body mass index (BMI) on irritable bowel syndrome (IBS) in Chinese adolescents.MethodsThe students were randomly selected from middle school and high school. They completed the epidemiological questionnaire of IBS and had their height, body mass measured.ResultsIn total, 1173 students were recruited, including 700 middle school students and 473 high school students. The incidence of overweight/obesity was 30.52%. The incidence of overweight/obesity was not significantly different between boys (28.40%) and girls (32.65%) (P=0.114), and there was not significantly different between students from middle school (30.57%) and high school (30.44%) (P=0.963). Two hundreds and twenty-one students suffered from IBS and the incidence of IBS was 18.84% with no significant gender difference. The incidence of IBS was significantly higher in students from middle school (13.43%) than those from high school (26.85%) (P<0.001). The incidence of IBS was not statistically significant be-tween overweight/obese students (22.07%) and normal weight students (17.42%) (P=0.061). The distribution of IBS subtypes had no statistical significance between overweight/obese and normal weight students (χ2=0.91,P=0.823). ConclusionsOverweight/obesity and IBS were extremely prevalent in Chinese adolescents, but the correlation be-tween them remains to be confirmed.