There are many defects in traditional clinical medical education,Socratic method has been used in law and commerce education,and we find it has the feasibility and operation to use the method in clinical medical education.

70%). Imipenem was of the strongest activity against Pseudomonas aeruginosa with the susceptibility of 86.3% but cefetaxime and ceftriaxone were of the weakest with the susceptibility of 6.8% and 11.0%, respectively. Conclusion The clinically isolated drug resistant Pseudomonas aeruginosa strains are increasing year by year. Therefore, clinical doctors should choose antibiotics rationally in dealing with the P. aeruginosa infected patients according to drug resistance test.

Animals ; Botulinum Toxins ; adverse effects ; therapeutic use ; Bruxism ; drug therapy ; Dystonia ; drug therapy ; Humans ; Joint Dislocations ; drug therapy ; Sialorrhea ; drug therapy ; Temporomandibular Joint Disorders ; drug therapy

According to the related requirements of medical technology clinical application management, the specific implementation approaches of surgery physicians surgery permission management were made, including establishing surgery classification project directory library, developing surgery classification management system and the surgery permission declared, and audit program, and investigating to determine the steps of surgery permission owned by surgery physicians currently, and finding problems and solution measures in implementation surgery permission management. To prevent surgical risk, improve the quality of operation, and evaluate surgical doctors scientifically, it provides management experience and references.

Objective To evaluate the safety of laparoscopic resection for giant abdominal benign mass.Methods Six cases of giant abdominal benign mass(8～25 in diameter)underwent laparoscopic exploration and resection under general anesthesia from July 2005 to March 2006.Results The laparoscopic resection was accomplished in all the 6 cases.The operation time was 75～220 min(mean,135 min),the intraoperative hemorrhage volume was 20～100 ml(mean,55 ml),the duration for abdominal drainage was 1～3 d(mean,2 d),and the postoperative hospital stay,2～7 d(mean,4.3 d).There was no complications such as intestinal injury,postoperative bleeding,or abdominal infection.Follow-up visits for 1～9 months(mean,6.5 months)found no recurrence.Conclusions Laparoscopic resection of giant abdominal benign mass is a feasible and safe minimally invasive technique.

Objective:To investigate the prevalence of Cluster-B personality disorders in university students. Methods: 1. Personality Diagnostic Questionnaire (PDQ+4) was administered to a sample which consisted of 3140 university students to screen personality disorder subjects. Cluster-B personality disorder subjects were finally diagnosed by conducting bland semi structured interview using Personality Disorder Interview-IV (PDI-IV) four to six weeks after the first administration. Results: The total prevalence of Cluster-B personality disorders among 3140 subjects of university students was 1.21% with 38 subjects met the diagnostic criteria of Cluster-B personality disorders. One female subject met the criteria of Antisocial Personality Disorder (APD), 21 Borderline Personality Disorder (BPD), 20 Narcissistic Personality Disorder (NPD), and 8 Histrionic Personality Disorder (HPD). The prevalence of BPD, NPD and HPD were 0.67%, 0.64% and 0.25% respectively. Ten subjects met the criteria of more than one personality disorder; the comorbidity rate was 26.3%. Conclusion: The prevalence of Cluster-B personality disorder in university students is lower than in general population reported by foreign researchers.

OBJECTIVE To explore the changes in foot fungus mobility and its drug resistance for further etiology investigation and clinical treatment. METHODS Sabourand′s agar culture medium was used to culture fungi, ID identification strip was employed to identify the fungi and drug sensitive test was performed by disk diffusion test. RESULTS The incidence of Trichophyton rubrum infection was the highest (79.1%). The isolated fungi were relatively sensitive to amphotericin B (AMB, 98.9%) and itraconazole (ITC, 98.0%), and resistant to 5-fluorocytosine (5-FC, 22.1%). CONCLUSIONS Detection technique of fungal infection should be improved and anti-fungal medicine should be used reasonably according to the results of drug sensitive test so that the fungal infection, especially fungi-resistant infection could be reduced.

OBJECTIVE A new piezoelectric aptamer biosensor is developed for determination of IgE. The energy converters are 10MHz AT-cut quartz crystals with gold-coated electrodes. The anti-IgE aptamers are immobilized onto the surfaces of crystals gold electrodes by biotin-avidin method. METHODS The standard substance and serum were detected to find the limit of detection and specificity of the biosensor. RESULTS The piezoelectric immunoglobulin aptamer biosensor could complete the detection without nonspecific response. Under the optimized conditions, the experimental results showed that the piezoelectric biosensor had good response to IgE whose frequency shifts were linearly dependent on IgE concentration in different range. The piezoelectric aptamer biosensor had been used to detect IgE in serum, the analytical results given by this method were in satisfactory agreement with those given by chemoluminescence method, its correlation coefficient was 0.9924. CONCLUSIONS Piezoelectric aptamer biosensor for the determination of IgE is of high sensitivity, high specificity, high analysis speed, unnecessary labeling, simple operation, real-time detection, etc. It is suitable for detection of IgE and should be used for clinical detection.

OBJECTIVE To investigate the methylation status and mRNA expression of the estrogen receptors(ERs) gene promoter in acute leukemic patients and detect the protein expression in leukemia cell lines with or without treatment of 5'-aza-Dc.And to find out the possibility of promoters methylation profile of estrogen receptor gene as an early diagnosis biomarker in leukemia.METHODS With RT-PCR and MSP,evaluating ERs mRNA expression and status of methylation in 40 acute leukemia patients without treatment.With Western-blot,detecting protein expression in leukemic cell lines with or without treatment of 5-azaDc.RESULTS The protein expression was significantly enhanced in all of leukemic cell lines with 5'-Aza-Dc.ER?-A was inactivated and specifically methylated(97.5%;39/40) in most of the acute leukemic patients.CONCLUSIONS The promoter ER?-A is inactivated and specifically methylated(97.5%;39/40) in most of the acute leukemic patients.This study may provide a new direction method to study the pathogenic mechanism of leukemia,and indicates that ER?-A methylation could be a potential reference marker for leukemia diagnosis.

With the goal of practice teaching for laboratory medicine students, we focus on culti-vating students' clinical thinking and scientific thinking through setting up reasonable teaching task and designing the scientific graduation thesis, and try to develop a novel practice teaching mode for laboratory medicine students. Therefore, we do exploration and practice in the following aspects: orientation training, clinical practice, medical ethics education, clinical communication ability, and scientific research design, hoping to improve students' research thinking ability and innovation ability. Through actual operation and graduation thesis writing, we try to help students to establish and improve their clinical thinking ability and innovation ability.