2.Evaluation of regional myocardial mechanical dysfunction of left ventricle in female patients with ECG T-wave inversion using velocity vector imaging
Chinese Journal of Ultrasonography 2013;(4):286-291
Objective To evaluate regional myocardial mechanical dysfunction of left ventricle in patients with ECG T-wave inversion using echocardiographic velocity vector imaging.Methods One hundred nineteen female patients with T wave inversion divided into three groups:V1-6 T-wave inversion group(52 patients),V1-4 Twave inversion group (33 patients),V3-6 T-wave inversion group (34patients),while selected 44 healthy women in control group.Peak systolic radial strain (SRSmax) and strain rate (SRSrmax),peak systolic circumferential strain (SCSrmax) and strain rate (SCSmax) were derived and compared from three short-axis views of left ventricle at 18 wall segments using a dedicated Syngo VVI software and workstation,and the depth of T-wave inversion in different precordial lead was measured and correlated with mechanics parameters between four groups.Results In V1-6 T-wave inversion group,each mechanical variable of anteroseptal,anterior,anterolateral and inferolateral walls decreased (P <0.05-0.01).In V1-4 T-wave inversion group each mechanical variable of anteroseptal,anterior and inferoseptal walls declined (P <0.05-0.01).In V3-6 T-wave inversion group four mechanical variables(i.e.,SRSmax SRSr SCSmax and SCSrmax) of anteroseptal,anterior,anterolateral and inferolateral walls reduced,mainly at basal segment (P <0.05-0.01).The ventricular segments of reduced SRSmax,SRSrmax and SCSrmax in V1-6 T-wave inversion group and of SRSrmax in V3-6 T-wave inversion group were more than those in V1-4 T-wave inversion group (P <0.05-0.01).In V1-6 T-wave inversion group,negative correlationships between radial mechanical variables of anteroseptal wall and the depths of V4 and V5 lead T-wave inversion were found (r =-0.29--0.37,P <0.05-0.01).Otherwise a positive correlationships between SCSmax of anterolateral wall and the depth of V3 lead T-wave inversion were established (rs =0.44,P =0.01) in V1-4 T-wave inversion group.Conclusions There is a regional myocardial dysfunction of LV in female patients with ECG T-wave inversion.The extent of electrical repolarization abnormality is correlated to the severity of regional myocardial dysfunction.
7.Amelioration of heart function by intracoronary autologous bone marrow mononuclear cells transplantation in idiopathic dilated cardiomyopathy: a clinical study
Yanjun CHEN ; Yao ZHANG ; Xinhua YIN
Chinese Journal of Interventional Cardiology 1993;0(02):-
0.05). No procedure related complications and malignant arrhythmia were found during 6-12 months follow-up. Conclusion Intracoronary transplantation of ABMMNCs is safe and may improve the heart function.
8.Screening for differentially expressed genes among human hepatocellular carcinomas,cirrhotic liver tissue and normal liver by genechip
Juan LIU ; Shukun YAO ; Fei YIN
Basic & Clinical Medicine 2006;0(01):-
Objective To compare gene expression profiles among hepatocellular carcinomas(HCC),cirrhotic liver tissue and normal liver and to analyze their biological information.Methods Total RNA was isolated from hepatocellular carcinomas,cirrhotic liver tissue and normal liver respectively,purified into mRNA by oligotex,and reverse-transcribed to synthetize two kinds of fluorescences(Cy3-dUTP,Cy5-dUTP) labeled cDNA probes.The targets were mixed together and hybridized with the genes on the genechip containing 4096 genes.The following comparisons were performed: hepatocellular carcinomas versus normal liver,cirrhotic liver versus normal liver.Genes that were differentially expressed between these groups were identified based on signal-to-noise ratios by using GenePix Pro3.0 software.Results 141 genes were found with close relation to HCCs,some of these genes were related to cell cycle and signal transduction,such as MCM7,YWHAH,among these,there are 23 genes whose expression tendency consistent with that of cirrhotic liver's.When compared with normal liver,there were 2 genes belong to metallothionein family downregulated in HCCs but upregulated in cirrhotic liver.Conclusion The inactivation of anticancer genes,the abnormality of metabolism related genes and the activation of cell proliferation cycle related genes may have a vital role in HCC.Research into these genes systematically may help to explore new markers of early diagnosisand to guide treatment proposal and to evaluate prognosis better.2007.27(1)
9.Sodium ferulate inhibits neurons injury mediated by A?_(25-35)-induced macrophage activation of mice
Suyan YAO ; Yin JIN ; Deyu ZHENG
Basic & Clinical Medicine 2006;0(01):-
Objective To examine the effect and mechanism of sodium ferulate inhibiting P38 MAPK in macrophage on neurotoxicity which was mediated by fribrilar-amyloid-induced.Methods The isolated peritoneal macropohages were cultured.P38 MAPK protein in nuclear extracts was analyzed by Western blot and production of tumor necrosis factor-?(TNF-?) was detected by ELISA.For neuron-macrophage co-cultures,Microtubute-associated protein-2(MAP-2) expression was detected by immunocytochemical technique.The level of LDH in the medium was measured.Results The production of TNF-? and P38 MAPK protein in nuclear extracts increased significantly by incubation with A?25-35.LDH efflux in neuron-macrophage co-culture medium increased and MAP-2 expression decreased significantly(P
10.Determination of expression of tyrosine protein kinase in hepatocellular carcinoma by tissue chip
Shukun YAO ; Junyan ZHAO ; Fei YIN
Chinese Journal of Hepatobiliary Surgery 2008;14(8):544-547
Objective To study the expression of tyrosine protein kinase in hepatocellular carcinoma(HCC)and observe the correlation between tyrosine protein kinase and clinical features.Methods A total of 30 cases with HCC were enrolled in this study.ERK,P38,C-jun,JAK2,STAT3 and STAT5 were detected by immunohistochemical method using tissue chip technology.Results The expression of ERK(0.220±0.033),P38(0.174±0.024),C-jun(0.183±0.064),JAK2(0.192±0.044),STAT3(0.197±0.078)and sTAT5(0.181±0.066)in HCC was significantly higher than that(0.065±0.028,0.058±0.028,0.042±0.016,0.070±0.030,0.052±0.024,0.052±0.023)in cirrhosis 1iver tissues(P<0.01).There was significantly positive correlation of the expression between ERK,C-jun,JAK2,STAT3 and STAT5(P<0.01 or P<0.05).But the expression of P38 was negatively correlated with ERK in the HCC tissues(r=-0.404,P<0.05).JAK2 had significant correlation with tumor differentiation.The expression of J AKz in stage Ⅲ was significantly higher than that in stage Ⅰ and Ⅱ cancer tissues.Conclusion There is important significance of the excessive activation of MAPK and JAK-STAT signaI transduction in hepatocellular carcinoma process.The unbalance of signal transduction might be one of the pathogenesis of tumor progress.