ObjectiveTo observe the effects of high mobility group box-1 protein ( HMGB1 ) on function of murine spleen IL-10-producing dendritic cells (DCs) subset CD11clowCD45RBhighDCs.MethodsSplenic CD11clowCD45RBhighDCs and CD4 +T cells in Balb/c mice were purified by magnetic beads sorting.CD11clowCD45RBhighDCs were treated with various doses of HMGB1 (0,20,100,500ng/ml).Flow cytometry was used to determine expressions of CD11clowCD45RBhighDC surface molecules including CD40,CD80,CD86,Ⅰ-a/e and Toll-like receptor (TLR) 4.IL-10 level in CD11clow CD45RBhighDC culture supernatants was determined by ELISA method.The CD4+ T cells were divided into four groups: control group (without only treatment),untreated group (HMGB1-untreated CD11clow CD45RBhigh DCs plus CD4+T cells),high-dose HMGB1-treated group (500 ng/ml HMGB1-treated CD11clowCD45RBhigh DCs plus CD4 +T cells),high-dose HMGB1-treated + antibody 1 group (500 ng/ml HMGB1-treated CD11clowCD45RBhigh DCs plus IL-10 antibody and CD4+T cells),high-dose HMGB1-treated + antibody 2 group (500 ng/ml HMGB1-treated CD11clow CD45RBhigh DCs plus homotype IL-10 antibody and CD4 +T cells).IL-4 as well as interferon( IFN-γ) contents in CD4 + T cell culture supernatants were determined by flow cytometry.ResultsHMGB1 could markedly enhance the expressions of CD40,CD86 and TLR4 on CD11clowCD45RBhigh DC surface as well as level of IL-10 secreted by CD11clow CD45RBhigh DCs as compared with the non-HMGB1 treatment.Meantime,the secretion of IL-10 was HMGB1 concentration dependent.IFN-γ level in high-dose HMGB1-treated group was (279 ± 17) pg/ml,which was markedly lower than that in the untreated group [ (963 ± 11 ) pg/ml,P ＜ 0.05 \ ].IL-4 level in high-dose HMGB1-treated group was (372 ± 14) pg/ml,which was significantly higher than that in the untreated group [ (213 ± 10) pg/ml,P ＜0.05) \].ConclusionsHMGB1 promotes IL-10 production in CD11clowCD45RBhighDCs,induces the differentiation of CD4 +T cells to Th2 cells and downregulates the immune response via activating CD11clowCD45RBhigh DCs.

Objective To compare the treament to adhesion ileus between laparoscopic surgery and traditional surgery.Methods 59 cases of patients with adhesion ileus were selected.32 patients were given laparoscopic adhesion ileus loose solution method.And the other 32 patients were given traditional surgery.Results The operation time(t =12.135,P =0.002),peristalsis recovery time (t =9.053,P =0.001),postoperative infection rate (x2 =8.346,P =0.004),obstruction again (x2 =4.863,P =0.026) and hospital stays (t =4.724,P =0.028) in laparoscopic surgery group was significantly better.The differences between both groups were statistically significant (P ＜0.05).Conclusion Compared with the traditional open surgery,laparoscopic adhesion ileus loose solution with surgical trauma is small.Operation time and the postoperative recovery time is short,It can be used as the main treatment of adhesion ileus.

Differentiating bipolar disorder (BD) from unipolar depression (UD) is an important clinical challenge.Review the development of Magnetic Resonance Imaging ( MRI) in distinguishing the BD and UD, identifying objective markers of BD, to optimize clinical decision making.Database including PubMed,Wan Fang,CNKI and so on.The key words were usedunipolar depressionormajor depressive depression,bipolar depression,MRI,modeland so on.A little neuroimaging studies to date have directly compared UD and BD depressions.Most results from these studies suggest more heavy neural circuit abnormalities in BD than UD depression,involved in different brain regions.Predictive models based on neu-roimaging characteristics of BD and UD obtain a higher accuracy and can help differentiate BD from UD.This review serves as a call to highlight the need for more neuroimaging studies to compare individuals with BD depression with individuals with UD depression directly.Using neuroimaging results as objective biological i-dentification markers is a feasible research field.

The principle of R0 resection for colorectal cancer(CRC)has been widely accepted based on the preoperative imaging evaluation.The surgeons should scientifically and strictly assess resectability of tumors and furthest reduce tumor residuals.Patients with initial unresectable CRC will give priority to conversion therapy and then undergo R0 resection if converting into resectable tumors.Palliative operation for the patients who cannot receive R0 resection according to the clinical assessment should strictly follow surgical indications and achieve wellplanned and clear surgical purposes.There is no evidence that the palliative operation or cytoreductive surgery benefits to survival of patients,and decreasing complications and improving quality of life through minimum wound are the basic principles.

Objective To investigate the clinical manifestations, histopathological patterns and causes of misdiagnosis of primary malignant tumor in small intestine (PMTSI). Methods The clinical data of 90 cases of PMTSI confirmed by pathology admitted from 1981 to 2002 in our hospital were retrospectively analyzed. Results The main pathological patterns of PMTSI were adenocarcinoma, leiomyosarcoma, lymphoma and malignant neurilemmoma. The common clinical manifestations were abdominal pain, anemia and hemorrhage, jaundice, weight loss and intestinal obstructions. Preoperative diagnosis rate was 22.2%(20/90). It was easily misdiagnosed as other digestive tract diseases and pelvic disorders. Preoperative misdiagnosis rate was 77.8%. Conclusions The main pathological patterns of PMTSI are adenocarcinoma, leiomyosarcoma, lymphoma and malignant neurilemmoma.Its clinical manifestations and diagnostic examination methods are not good enough, which usually leads to misdiagnosis ,so attention must be paid.

Janus kinase-signal transduction and transcription activator (JAK-STAT), mitogen- activated protein kinase (MAPK) and nuclear factor ?B (NF-?B) are three important cellular signalling pathways, which play pivotal roles in regulation of cellular physiologic as well as pathophysiologic functions. Based on the elucidation of the research progress of three signalling cascades, respectively, the current review focuses on the cross-talk of these signalling transduction, and the up-to-date details are also presented on their regulation in inflammatory response.

Leptin,a polypeptide hormone coded by Ob-gene,has the functions of depressing appetite,reducing caloric intake and enhancing caloric consumption,etc.Leptin has a close relationship with kidney,for it is metabolized and eliminated via kidney.Meanwhile,leptin can also directly affect kidney functions,influencing the process of chronic renal failure.Furthermore,hyper-leptinemia is associated with anorexia,malnutrition and cardiovascular diseases.Different dialysis patterns may also play different roles in cleaning leptin.For example,common dialysis could not clear leptin,but other dialysis modes such as high permeability dialysis,hemodiafiltration,and mastic absorption hemoperfusion can alleviate hyper-leptinemia through cleaning leptin partly.For kidney transplantation patients,hyper-leptinemia may affect the immune balance which has been maintained for a long time.

This article proposes the integrated outline of excessive heat accumulated in chest syndrome from the view of Fu-viscera disease of sanjiao based on numbers of clinical experiences,which can prove that the objective existence and theory of sanjiao fu-viscera has specific significance.

Objective To investigate the inhibitory effect of RNA interference(RNAi)on WT1 gene expression in HL-60 cell line.Methods Several pairs of small interfering RNA(siRNA)for WT1 gene was designed and transfected into HL-60 cells.WT1 gene expression was assessed by RT-PCR,and WT1 protein expression was tested by Western blot.Flow cytometry was used to measure the cell apoptosis.Results The expression rates of WT1 mRNA and the expression rates of WT1 protein were(23.62%?1.28%),(23.20%?1.04%),respectively in HL-60 WT1 siRNA-transfected cells,significantly lower than those of control cells(P

OBJECTIVE:To discuss the status quo and the development strategy of the study of the processing of Chinese herbal medicine.METHODS:The physical-chemical property,the technology transfer and the qualitation and quantitation reported in the literature about the processing of Chinese herbal medicine were analyzed and summarized.The status quo and the current methods for the study of processing of Chinese herbal medicine were expounded and its development strategy was put forward in view of the role and importance of the processing study.RESULTS & CONCLUSION:To develop the study on the processing of Chinese herbal medicine,it is essential to strengthen literature research and summarizing of experiences as well as the combination of processing synergy with clinic practice,attach great importance to the guiding effect of traditional Chinese medicine theory on the study of physical-chemical study in the processing of Chinese herbal medicine,and strengthen study on the key issues including the processing technology of Chinese herbal medicine and the quality control of decoction pieces.