Objective An intensive analysis on the recent advances in cardiovascular medicine both in China and abroad was made,so as to provide an orientation of developing cardiovascular medicine in the future.Methods Literature of cardiovascular medicine published domestically and abroad in recent 5 years was reviewed.The advances in the use of new drug and techniques,clinical diagnosis and therapeutic methods were analyzed.Results Cardiovascular medicine in basic,clinical and practical fields had made rapid advances in the military institutions lately,especially in the interventional diagnosis and treatment of coronary heart disease,arrhythmia and peripheral vascular disease,and in the treatment of hypertension,where outstanding successes had been achieved.Conclusions The general level of cardiovascular medicine of military medical establishments could be considered foremost in our country.During the coming years of "the 12th Five-Year Plan",the emphasis of research work should be placed upon the medical care,basic and clinical research of common cardiovascular diseases existing in military officers and soldiers,such as coronary heart disease,peripheral vascular disease and arrhythmias,both in peace time and military conflicts.

mia and hypertension are the major risk factors for occurrence of diabetic retinopathy.High UA may be also associated with occurrence of diabetic retinopathy.

Objective To study the effect of rising [Ca 2+ ]i on the expression of Caspase 3, Bax and Bcl 2 in human breast cancer MCF 7 cell lines. Methods After the MCF 7 cells treated with different dose of thapsigargin(TG), intracellular calcium concentrations in MCF 7 cells were measured by calcium sensitive fluorescence indicator Fura 2/AM technique; and immunohistochemical method was used to detected the expressions of Caspase 3, Bax and Bcl 2. Results Bax was over expressed and Bcl 2 was down expressed in a [Ca 2+ ]i concentration dependent way;nevertheless, the expression of Caspase 3 was negative in all the TG treated cells. Conclusions The increase of intracellular calcium concentration may have an inductive effect of apoptosis on MCF 7 cells, which is independent to Caspase 3.

Objective To study the apoptotic induction effect of Thapsigargin on estrogen receptor positive human breast cancer cell lines MCF 7. Methods Cells were treated with Thapsigargin and 5 FU in vitro . The rate of cell apoptosis and distribution of cell cycle were detected on flow cytometry. The cell viability was measured by MTT assay and ultrastructural changes in apoptotic cells were confirmed by transmission electron microscopy.Results Thapsigargin could increase the rates both of cell apoptosis and growth supression of MCF 7 cells induced by 5 FU and alter the distribution of cell cycle. Under electron microscope, apoptotic bodies in MCF 7 cells considerably increased.Conclusion Thapsigargin apparently enhances the effect of apoptotic induction of 5 FU on MCF 7 cells, it is worthy of being further studied.

The freshmen column in 39 985 Projectacademic libraries was investigated , which showed that 15 out of the 39 985 Projectacademic libraries have established freshmen column on their library website .Its posi-tion, contents and forms were analyzed.The typical freshmen column in libraries of Qinghua University, Shanghai Jiaotong University , and Sun Yat-sen University was described with suggestions put forward for improvement of its position, contents and forms.

Adiponectin ; Ghrelin ; physiology ; Growth Hormone-Releasing Hormone ; Humans ; Leptin ; Receptors, Ghrelin ; Resistin

Objective To investigate the effects of hydroxyethyl starch(HES 130/0.4)on leukocyte activation,mast cell degranulation and vascular permeability in rat mesentery during the early phase of endotoxemia.Methods Thirty-six male Wistar rats weishing 200-250 g were randomly divided into 3 groups(n=12 each):Ⅰ normal control group received only normal saline(NS);Ⅱ LPS group and Ⅲ HES group received LPS 2 mg/kg iv followed by iv HES infusion at 16 ml·kg-1·h-1 for 60 min(HES group)or equal volume of NS(LPS group).The animals were anesthetized with intramuscular 20% urethane 1 ml/100 g.The abdomen was opened and the mesentery of small intestine was pulled out and placed on transparent observation plate at constant temperature and moisture and examined under microscope for microcirculatory changes.Leukocytes rolling along,adhering to and emigrating from mesenteric venules,mast cell degranulation and FTTC-albumin effux from venules were examined before(baseline)and during the 90 min after LPS administration.The expression of CD11b and CD18 on the leukocytes was determined using flow cylometry.Results The number of leukocytes rolling along,adhering to and emigrating from venules and degranulated mast cells were signitlcanfly increased in LPS group as compared with control group.These LPS-induced changes were significantly inhibited in HES group.The albumin effiux Was enhanced and CD11b/CD18 expression upregulated in both LPS and HES groups.Conclusion Hydroxyethyl starch(130/0.4)can ameliorate the increased vascular permeability and microcirculatory disturbance in rat mesentery during the early phase of endotoxemia.

The conformity between bone implant and bone tissue plays an important role in osseointegration.Protein molecules such as extracellular matrix proteins,cytoskeletal proteins,and attachment proteins,which produced by osteoblasts,promote the attachment of osteoblasts to bone implant and the surrounding ceils.In vitro studies show that nano-plated implant material bears excellent biocompatibility and superior physical and chemical features.Nano-scale material has the advantage of high surface roughness,wettability and electrochemistry features which erdaance the adherence and interaction of extracellular matrix proteins and promotes the attachmerit,proliferation and differentiation of osteoblasts.The expression of cytokines,such as prostaglandin E2(PGE2)and transforming growth factor-β(TGF-β)are also increased in nano-structured bone implants which in turn promote the new bone formation.Further studies need to be done to explain the mechanism of the effect of nano-tructured bone implant on osteoblasts.

Objective To identify risk factors for anastomotic leakage after low anterior resection (LAR) in rectal cancer patients and study its impact on patients'long-term prognosis.Methods Chnical data were analyzed from 720 patients who underwent low anterior resection(LAR) for rectal cancer between 1996 and 2006.Results Anastomotic leakage after anterior resection occurred in 44 cases(6.1%).The median time of anastomotic leakage after operation was 5.6 days.Muhivariate analysis showed male patients.history of preoperative chemoradiation therapy,diabetes,cancer distance from anus less than 7 cm and hand-sewed anastomosis were independent risk factors predisposing anastomosis leakage (P<0.05).Tumor local recurrence rate was 13.6% in patients suffering from leakage and 5.9% for those without leakage (χ2= 4.116,P<0.05).The distant metastasis rates were 25.0 and 14.1 percent for the leakage and noaleakage groups,respectively(χ2=3.943,P<0.05).The survival rates were 56.8 and 72.5 percent in the leakage and nonleakage groups,respectively(χ2=4.979,P<0.05).Conclusion Sex,preoperative chemoradiation therapy,diabetes,cancer distance from anus less than 7 cm and hand-sewed anastomosis were found to be independent risk factors for anastomotic leakage after rectal cancer surgery.Anastomotic leakage was associated with poor prognosis.

Objective To investigate the effect of single operation Mammotome system on breast benign tumor patients.Methods 86 cases of benign breast cancer patients admitted from Jan.2011 to Dec.2015 were divided into the control group (43 cases) and the observation group (43 cases) according to the random number table method.The control group were treated by traditional breast lesion resection and the observation group were treated by single operation of Mammotome system.The mean time of operation,the average blood loss and the size of surgical scar were recorded.The number of patients with deformity of breast and complications was recorded.The satisfaction of the two groups was evaluated by questionnaire.The recurrence rate of the two groups was statistically analyzed.Results The average operation time of the observation group was significantly shorter than that of the control group,and the scar size was significantly smaller than that of the control group (P＜0.05).The deformity rate of the observation group was 0％,significantly lower than that of the control group (11.6％).The satisfaction rate was 97.7％ in the observation group,significantly higher than that of the control group (83.7％,P＜0.05).The postoperative complication rate was 4.7％ in the observation group,significantly lower than that of the control group (18.6％,P＜0.05).There was no recurrence in the two groups during 6 months of follow-up.Conclusion The application of single breast operation Mammotome treatment for benign breast tumor has the advantages of simple operation,shorter operation time,less intraoperative blood loss and surgical scars.It also can reduce the postoperative complications,keep breast shape,reduce the recurrence rate,so as to improve the patient satisfaction.