1.Research progress in topical application of anti-vascular endothelial growth factor drugs for uveitis-associated cystoid macular edema and choroidal neovascularization
Chinese Journal of Experimental Ophthalmology 2014;32(7):659-663
Uveitis is a kind of inflammatory disease affected in ocular posterior segment.Uveitis sustains a long duration and causes a significant damage of visual function.It often leads to serious complications,such as cystoid macular edema (CME),cataract,glaucoma,retinal detachment and choroidal neovascularization (CNV).In the pathological process of uveitis,inflammatory factors promote the release of vascular endothelial growth factor (VEGF) through a variety of pathways.It has been verified that VEGF level is elevated in vitreous in the patients with uveiticinduced CME.Intravitreal injection of anti-VEGF drugs,including ranibizumab and bevacizumab,can improve the visual acuity in most patients with noninfectious uveitic-induced CME and CNV.However,intravitreously repeated injections of anti-VEGF drugs may be required in some patients.Intraocular injection of anti-VEGF drugs is an optional and effective way for those with persist macular edema and not being suitable for the use of corticosteroid or immunosuppressive therapy.However,anti-VEGF drugs may affect the efficacy of corticosteroid.Therefore,more clinical and basic researches are still needed.The status and progresses in the use of anti-VEGF drugs for uveitisinduced CME and CNV were reviewed.
2.Correction of Angle class Ⅱ division 1 malocclusion by means of segmental approach
Yufen QIAN ; Xin GONG ; Yao GONG
Journal of Practical Stomatology 2001;17(3):191-193
Objective:To evaluate the effect of segmental approach in the treatment of Angle class Ⅱ division 1 malocclusion.Methods:7 cases of classⅡ,division 1 maloclusion were treated.Upper first premolar and lower second premolars were extracted in all cases.Lower first molars were mesially moved by means of segmental arch. Cephalometric analysis was used to evaluate the effects of the treatment.Results:Facial profile improvement and lower anterior facial height maintenance were achieved.The occlusion plane angle,mandibular plane angle were well controlled with lower anterior teeth upright on the lower basal bone.All patients represented good mandible response.Conclusion:Segmental technique is a simple and effective approach in correcting class Ⅱ,division 1 maloclusion
3.Effect of 15-HETE on the hypoxic pulmonary arterial rings
Liwei MENG ; Xiuping GONG ; Hongjuan YAO
Chinese Journal of Anesthesiology 1994;0(01):-
Objective To inveatigate the effect of 15-HETE, a metabolite of arachidonic acid, on isolated hypoxic pulmonary arterial ringa ( PARs) , trying to find appropriate treatment for pulmonary hypertension and its complications during anesthesia in order to avoid hypoxemia. Methods Sixteen healthy Wistar rats of either sex weighing (230 ? 10) g were randomly divided into two groups : A control group breathing fresh air (FiO2 =21%) and B hypoxia group breathing hypoxic air (N2 = 90% , O2 = 10% ) in a hypoxic box. After breathing hypoxic air for 9 days the animals were anesthetized. Heart and lungs were immediately removed and PARs (0.5-1.0 mm in diameter and 3 mm in length) were prepared. Four PARs were prepared from each animal. The PARs were suspended in baths filled with Krebs-Hensleit (K-H) solution maintained at 37℃ and aerated with 95% O2 and 5% CO2. Preload was gradually increased to 0.3 g in 30 min. The isometric tension was measured using a four-channel force-displacement transducer. 15-HETE was added to K-H solution and the concentration was gradually increased from 10-8 to 10-6 mol?L-1 at 5 min intervals. Contractility of PARs was analyzed by a software of Medlab 6.0. Concentration-tension curve was drawn and contraction rates were calculated. 2 mmol?L-1 4-AP, 10-2 mol?L-1 TEA and 10-6 mol?L-1 GLYB were added to separate K-H solution baths and 40 min later 15-HETE was added in order to detennine the effect of difierent potassium channel blockers on contraction response of PARs to 15-HETE. Results With increasing concentration from 10-8 to 10-6 mol?L-1 , 15-HETE increased PARs tension gradually in a dose-dependent manner from 106% ?6% to 139% ? 4% in group A and from 113% ?6% to 163% ?6% in group B. The difference in PARs tension between group A and B was statistically significant (P
4.Combined lamivudine and hepatitis B immunoglobulin to prevent hepatitis B recurrence after liver transplantation:a Meta-analysis study
Liqing WEI ; Yao ZHANG ; Haiyan GONG
Journal of Third Military Medical University 2003;0(10):-
Objective To evaluate the efficiency of lamivudine combined with hepatitis B immunoglobulin to prevent hepatitis B recurrence after liver transplantation.Methods The literature concerning the application of lamivudine and hepatitis B immunoglobulin after liver transplantation was collected.The efficacy of initial lamivudine,and hepatitis B immunoglobulin alone or combined together was evaluated in liver transplantation recipients with hepatitis B by performing a systematic review of the literature with a Meta-analysis of clinical trials.Odds ratio(OR)was applied to evaluate the effect of therapeutic alliance to decrease the reinfection rate whether or not.Results We identified 7 clinical trials,and there were 360 patients subjected.OR and 95% confidence interval(95%CI)was 0.34(95%CI ranging from 0.18 to 0.64).For overall test result,Z value was 3.33 and P value was 0.01.The P value was 0.310 for our test of study homogeneity.Conclusion Our meta-analysis shows that lamivudine or hepatis B immunoglobulin can effective prevent hepatitis B recurrence after liver transplantation,and therapeutic alliance is more effective than monotherapy,and tolerance to lamivudine or hepatis B immunoglobulin was good.
5.Effect of soybean isoflavones on ventricular remodeling induced by myocardial infarction in rats
Yue LI ; Minghui YAO ; Yingqing LU ; Qinyan GONG
Chinese Journal of New Drugs and Clinical Remedies 2006;25(4):241-247
AIM: To investigate the effect of soybean isoflavones (SI) on ventricular remodeling induced by myocardial infarction (MI). METHODS: MI was induced by permanent ligation of the left anterior descending coronary artery (LAD) in male Sprague-Dawley (SD)rats. Rats were randomly divided into six groups: shamThree hours after the operation, the drugs or solvent were administrated ig qd for 35 d. Twenty-four hours after the last administration, heart rate (HR), left ventricular end-diastolic pressure (LVEDP), peak systolic left ventricular pressure (Peak), maximal rate of left ventricular pressure rising (+dp/dtmax), maximal rate of left ventricular pressure declining (-dp/dtmax) and myocardial maximal shortening velocity (Vmax) were measured. After above hemodynamic parameters were measured, hearts were extracted. The ratio of total ventricle weight to body weight(TVW/BW) was calculated. Myocardial collagen was shown with the collagen-specific picrosirius red stain,myocardial interstitial collagen volume fraction(ICVF), perivascular collagen volume fraction(PCVF), infarct size,septal thickness (ST) and left ventricular diameter (LVD) were measured by image analysis system. RESULTS:(31 ± 5) %, respectively, all of which were significantly smaller than that in MI group (( 38.9 ± 2.9) %, P < 0.01 ).In MI group, TVW/BW, LVD, ICVF and PCVF were remarkably increased compared with those in sham group (P < 0.01 ). The increased TVW/BW, LVD, ICVF and PCVF were significantly reduced by treatment with eaprats in MI group was significantly decreased than that in sham group (P < 0.01 ). The reduced ST could be in-dp/dtmax and Vmax were decreased (P < 0.01 ), while LVEDP was increased (P < 0.01 ), significantly. The reSION: SI can improve cardiac function and ventricular remodeling induced by myocardial infarction in the rat.
6.Effect of genistein on c-myc mRNA expression induced by low density lipoprotein in endothelial cells
Jin LIU ; Minghui YAO ; Qinyan GONG ; Rong YU ; Yingqing LU
Chinese Journal of New Drugs and Clinical Remedies 2003;22(1):5-8
AIM: To study the effect of genistein on c-myc mRNA expression induced by oxidized low density lipoprotein (ox-LDL) in human vascular endothelial cells (ECV304). METHODS: LDL were isolated from healthy human plasma by gradient ultracentrifugation and oxidized by CuSO4. ECV304 cells were exposed to ox-LDL 200 mg*L-1 in the presence or absence of genistein 100 μmol*L-1 for 1, 2, and 4 h in vitro. Northern blot was employed to measure c-myc mRNA levels of ECV304. RESULTS: In response to ox-LDL 200 mg*L-1, c-myc mRNA expression in ECV304 increased by 3 fold for 1h and 3.3 fold for 2 h and decreased below the control level at 4 h. Expressions of c-myc stimulated by ox-LDL in the presence of genistein 100 μmol*L-1 for 1 h and 2 h were separately 80 percent and 60 percent of that in the absence of genistein 100 μmol*L-1. CONCLUSION: Genistein can effectively inhibit c-myc mRNA expression in ECV304 induced by ox-LDL.
7.Research on failure mode and effect analysis in endoscope disinfection management
Kailan ZHENG ; Xiaohua GONG ; Dongying YAO ; Cuixiang YU
Chinese Journal of Practical Nursing 2011;27(20):59-61
Objective To identify and investigate the cause of the unacceptable bacteria level inside the lumen of fiberendoscope during monitoring so as to increase the pass rate of its cleaning and disinfection. Methods A failure mode effect analysis of the cause was done and the workflow was thereafter improved. Meanwhile, pre and post bacteriological monitor was applied to the fiberendoscope lumen samples. Results The number of scrubbing times while manual cleaning affected the monitoring results. The scrubbing times was amended and the pass rate increased from 84.15% to 98.65%. The results were significantly different The failure mode risk index of endoscope cleaning and disinfection was calculated, and the highest two items were: The number of scrubbing times the operators execute RPN=640, the method of lumen cleaning RPN=480. These were the key factors of qualification of monitoring. Conclusions After the amendment, no less than 5 times' scrubbing was required and the bacteriological monitoring method was used to analyze the effects,thereafter the pass rate of lumen bacteria monitoring can be increased significantly.
8.Risk factors for symptomatic steno-occlusive carotid disease
Yao LI ; Xiping GONG ; Yongjun WANG ; Zheng LI
Chinese Journal of General Practitioners 2010;9(4):270-272
One hundred and fifty patients with 70 percent or more of carotid steno-ocelusion confirmed by digital subtraction angiography were selected to analyze risk factors for its clinical symptoms.Results of analysis showed that risk for clinical symptom increased with severity of unilateral carotid stenosis (OR = 3.546,95% CI 1.515-8.300,P = 0.004).Whereas presence of "functional complete circle of Willis" was a protective factor for it (OR = 0.208,95 % CI 0.045-0.962,P = 0.045).
9.Influences of two different HLA-B antigens on receptors expression of NK cells from peripheral blood lymphocytes
Weijuan GONG ; Yuqin YANG ; Fangjuan YAO ; Lingdi XU ; Lian FAN
Chinese Journal of Immunology 2001;0(10):-
Objective:To investigate influences of two different HLA-B antigens expressed on K562 cells on receptors expression of NK cells from peripheral blood lymphocytes.Methods:Studied the alteration of the percentage of CD16+CD56+ cells and the percentage of KIR3DL1+ cells before and after PBMC interaction with K562 cells for 24 hours,and also compared the percentage of CD16+CD56+ cells and the percentage of KIR3DL1+ cells after PBMC interaction with two different kind of K562 cells transfected with HLA-B39 and HLA-B51 respectively.Results:After PBMCs were incubated with K562 cells for 24 hours,the percentage of CD16+CD56+ cells and the percentage of KIR3DL1+ cells were both increased.However,after PBMCs were incubated with K562-HLA-B51 cells for 24 hours,the percentage of KIR3DL1+ cells and the percentage of CD16+CD56+ cells were both decreased in comparison with that interaction with K562-HLA-B39 cells.Conclusion:CD16 up-regulation was associated with an up-regulation of inhibitory receptors(KIR3DL1).The interaction between HLA-Bw4 and KIR3DL1 would down-regulate the expression of KIR3DL1.In addition,KIR3DL1 down-regulation was associated with down-regulation of activating receptors(CD16).
10.Effect of chronic experimental stress on insulin sensitivity in susceptible mice to diabetes
Youshuo LIU ; Min HU ; Shuqiao YAO ; Yaoxian GONG
Chinese Journal of Geriatrics 2000;0(04):-
Objective This study was to compare the effects of chronic experimental stress and aging on insulin sensitivity at insulin receptor of liver plasma membranes and its autophosphorylation in the aged mice compared with those in the young and the adult diabetes-susceptible mice. Methods Diabetes-susceptible mice was induced by streptozotocin in 40 1.5-month-old mice (young mice), 40 15-month-old mice (adult mice) and 40 20-month-old mice (aged mice). These mice were randomly divided into the stress groups and control groups, respectively. Each group contained 20 mice. Stress groups were exposed to multiple stressors (restrain, rotation, crowding) for 6 weeks. The levels of fasting blood glucose (FBG) and fasting plasma insulin (FIns) were measured. The index of insulin sensitivity (ISI) was calculated. The numbers (R_~1 , R_~2 , R) and affinity (K_~1 , K_~2 , K) of insulin receptors on liver plasma membranes were investigated by using Scatchard analysis. The numbers of insulin-stimulated (10~-7 mol/L) phosphorylated insulin receptor (PINSR) on liver plasma membranes was assayed by ELISA. Results After 6-week stress, FBG levels were significantly higher with aging 〔young mice: (10.3?6.2)mol/L, adult mice: (15.2?3.6)mol/L, aged mice: (18.9?2.9)mol/L, P