Objective To investigate the expression characteristics of the phosphatase and tensin homolog deleted on the chromosome 10 (PTEN) in the central nervous system(CNS) of adult rats. Methods Immunohistochemical staining was used to demonstrate the PTEN-like positive immunohistochemical product. Results Expression of PTEN was extensive in the CNS. The distribution of positive cells was strong, moderate or weak and its subcellular localization included the cytoplasm, nuclei and processes. Conclusion The present results identify the expression of PTEN protein in the CNS of adult rats.

Objective To observe the regulatory mechanism of substance P on the tension of human umbilical vein and the calcium ion of endothelial cells. Methods The tension was recorded by conventional physiological recording methods,and confocal laser scanning microscopy and patch\|clamp technique were used to observe the concentration of intracytosolic free calcium ion and the opening probability of membrane calcium ion channel of cultured human umbilical vein endothelial cells. Results Substance P induced the endothelial\|dependent relaxing of human umbilical vein and the increasing of the concentration of intracytosolic free calcium ion and of the opening probability of calcium ion channel in the cultured human umbilical vein endothelial cells.Conclusion\ Substance P could be function as the activator of the releasing of the intracellular stored calcium ion,and the inflowing of calcium ion from the outside cell bodies to relax human umbilical vein.

Nuclear factor-?appa B(NF-?B) is an important transcription factor in the expression of any inflammatory media.The role of NF-?B in rheumatoid arthritis has been thought high recently.This review presents the role of NF-?B in rheumatoid arthritis,and the research progression of intervention therapy in inhibition of the activation of NF-?B is also introduced.

Objective To investigate the value of Xueshuangtong combined with radiofrequency thermocoagulation in the treatment of lumbar degenerative lumbar pain.MethodsFrom January 1, 2014 to December 30, 2015, 60 patients with lumbar degenerative low back pain admitted in Jiashan Traditional Chinese Medicine Hospital.All of them were randomly divided into observation group and control group, and each with 30 cases.All patients received radiofrequency thermocoagulation, while the observation group received Xueshuantong for further treatment.All of the patients were assessed by the international Pain Association recommended the use of visual analogue scale (VAS) and McGill pain questionnaire (SF-MPQ) and CRP when treatment before starting and after 1, 3, 7, 14, 30 and 90d of the treatment.ResultsThere were no significant differences between the two groups in VAS, SF-MPQ and CRP before treatment (P<0.05).However, when compared with the control group, patients in the observation group got significantly lower levels of VAS at 1, 3, 7 and 14d after the operation;significantly lower levels of SF-MPQ at 1, 3, 7, 14 and 30d after the operation;and significantly lower levels of CRP at 14, 30 and 90d after the operation (P<0.05).And we found no serious complications occurred in the two groups.ConclusionThe Xueshuantong combined with radiofrequency thermocoagulation treatment of lumbar facet joint is a safe, effective and inexpensive for the low back pain which caused by lumbar degeneration.

Rheumatoid arthritis ( RA) is a kind of autoimmune disease with high morbidity characterized in synovitis.It has become a key goal for achieving an early RA remission.It is very important for application of RA remission normatively in the treatment of RA.In this paper, comparison and application of various RA remission criteria were reviewed, such as American College of Rheu-matology (ACR), European League Against Rheumatism (EULAR), US Food and Drug Administration (FDA), Outcome Measures in Rheumatoid Arthritis Clinical Trials ( OMERACT) group, ACR /EULAR remission criteria, etc.

Objective: To investigate the effect of elemene on the drug resistance of cisplatin (DDP)-resistant human lung adenocarcinoma A549/DDP cells, and its possible mechanism. Methods: The growth inhibition of A549/DDP cells treated with elemene alone or combined with different concentrations of DDP was detected by MTT assay. The morphological changes of apoptosis of A549/DDP cells treated with different concentrations of elemene (20 and 40 μg/mL) for 24 h were observed by Hoechst 33342 staing under fluorescence a microscope. The apoptosis rate of A549/DDP cells treated with different concentrations of elemene (20 and 40 μg/mL) after 24 h were detected by FCM (flow cytometry). The expressions of cytochrome C, pro-caspase-3, caspase-3 and the Bcl-2 family proteins were measured by Western blotting analysis. Results: MTT result showed that different concentrations elemene could inhibit the proliferation of A549/DDP cells in a time- and dose- dependent manner. Intriguingly, elemene plus DDP enhanced the sensitivity of A549/DDP cells to DDP and reversed the resistance of A549/DDP cells. Elemene was also a strong inducer of apoptosis in this model system, and a synergistic effect on induction of cell death was observed when the tumor cells were treated with both agents. The result showed that elemene could enhance A549/DDP cell apoptosis. Furthermore, the combination of elemene and DDP also enhanced the protein expressions of cytochrome C, caspase-3 and Bad, and reduced the protein levels of Bcl-2 and pro-caspase-3 in the A549/DDP cells. Conclusion: The reversal of the multi-drug resistance of A549/DDP cell line by elemene may result from its effect on induction of apoptosis. Elemene treatment can impaire mitochondrial membrane, release cytochrome C into cytoplasm, activate caspase-3,up-regulate pro-apoptotic protein Bad and down-regulate anti-apoptotic protein Bcl-2, and finally caused apoptosis. Copyright © 2013 by TUMOR.

35 g/L) by serum albumin determination. Relationship between prognosis and BMI as well as serum albumin was observed. Results:Morbidity of malnutrition was as high as 31.3 %.Concentration of serum albumin was low in most patients. Postoperative complications occurred mainly in malnutrition and hypoalbuminemia group. Conclusions:BMI and serum albumin are related with patients' prognosis and can be used to estimate risks of operations.

Objective To study the dynamic changes and clinical significance of pulmonary function (PF) in segmental mycoplasma pneumonia (SMPP) children and make the dynamic analysis of X-ray features.Methods Eighty-three SMPP children treated from June 2013 to December 2014 in Jinshan Institute of the Sixth People's Hospital of Shanghai were selected and the PF changes at acute phase, recovery phase and rehabilitative period was monitored and all the patients received the imageological diagnosis.The Pulmonary function includes forced vital capacity (FVC), forced expiratory volume in the first second (FEV1), forced expiratory volume ratio(FEV1/FVC), peak expiratory flow(PEF), and maximum expiratory flow (FEF25-75).Pulmonary function and imageological diagnosis of two groups was compared.Results Compare the acute phase and recovery phase and rehabilitative period of 83 SMPP Children, at acute phase, FVC ((1.58±0.49), (1.76 ±0.62),(2.08±0.84) L),FEV1((1.27±0.46),(1.58±0.53),(1.83±0.66) L),FEV1/FVC((79.64±6.61)％,(85.25±7.38)％,(87.24±8.61)％),PEF((2.61±0.84),(3.15±0.92),(3.52±1.06) L/s), FEF25 ((2.29±0.83), (2.86± 0.95), (3.26± 0.98) L/s), FEF50 ((1.51 ± 0.52), (2.12 ± 0.64), (2.26±0.63) L/s),FEF75((0.58±0.42),(0.76±0.46),(1.02±0.42) L/s) and FEF25-75 ((0.61±0.33),(0.87±0.36), (1.01 ±0.41) L/s) of two groups were reduced, especially FEF25, FEF50, FEF75 and FEF25-75.At recovery phase and rehabilitative period ,the indicators were significantly better than those of acute phase (P＜ 0.05);for SMPP children at acute phase, FVC ((1.51 ± 0.44), (1.31 ± 0.36) L) and FEV 1 ((1.46±0.56), (1.21±0.48) L) in the lesions of multiple parts was significantly lower than that of lesions of single part(P＜0.05).At acute phase, X-ray majorly showed the unilateral lesion with increased density and blurring edges in lung lobe or segment.After 14 d, the condition was obvious improved;a few cases still had the blurring textures and thickening conditions after 2 weeks.Conclusion For SMPP children at acute phase,the major and micro airway function is damaged in different extent.It is majorly expressed as restrictive ventilation dysfunction,especially micro airway function.At recovery phase, PF is obviously improved.The damage of major airway function in the multiple parts of lung lobe is more serious than that of lesions in the single part.

Aim To study effects of vigabatrin on absence-like seizures and tonic convulsions in spontaneously epileptic rats (SERs). Method Electrcorticogram and depth electroencephalographic activity in hip- pocampus of SERs were recorded with implanted electrodes after administration of vigabatrin. Results The number of absence-like seizures was significantly reduced from 100% to (54?5)%, (41?9)% and (34?4)% (P

The dendritic cell(DC) has the most powerful function among the antigen presenting cells known hitherto,which stimulates naive T cells and plays a pivotal role in inducing T cell tolerance.The role of DC in rheumatoid arthritis(RA) has aroused more and more attention.This article reviews the role of DC in RA and the researches on the drug intervention in its treatment.