Objective To identify the stem cell characteristics of human diploid cells 2BS and evaluate the potential as biological function positive cells for quality detection of stem cells.Methods Flow cytometry was applied to detect the expression levels of stem cell surface markers in 2BS cells.The staining with alizarin red S,oil red O and alcian blue was used to evaluate the ability of osteogenic,adipogenic and chondrogenic differentiation,respectively.Co-culture with lymphocytes was used to observe the effects on lymphocyte proliferation.Results Human diploid cells 2BS expressed the surface markers CD73,CD 105 and CD90 of stem cells,and possessed the potential of osteogenic differentiation and inhibiting lymphocyte proliferation.Conclusion 2BS cells have several properties of stem cells and could be the positive control for surface markers detection of stem cells by flow cytometry.

IL-8 is a cytokine which belongs to the chemotactic superfamily.It is one of the major mediators in the inflammatory response.In recent years,it was found that it had multiple functions,such as chemoattractant,potent angiogenic,and so on.The cytokine is believed to play an important role in the pathogenesis of inflammation,tumor progress and immune disease.In this review,the properties of IL-8 and highlight its roles on inflammation and tumor progress were surveied.

Independent component analysis (ICA) aims at decomposing an observed random vector into statistically independent variables. Fast independent component analysis (FastICA) algorithm and its variants are catching more attention because of their simplicity and convergence speed. In this paper, a novel method referred to robust independent component analysis (RobustICA), based on normalized kurtosis and optimal step-size, is analyzed in detail. When applied to fetal electrocardiogram (FECG) extraction and compared with FastICA, it gave decent results and showed prosperous future usages.
Algorithms ; Electrocardiography ; Female ; Fetal Heart ; physiology ; Fetal Monitoring ; Fetus ; Humans ; Pregnancy ; Principal Component Analysis ; Signal Processing, Computer-Assisted

Objective: To study the color reproduction ratio of metal ceramic restoration in clinic. Methods:The color of 150 maxillary incisors and its metal ceramic restorations was measured by MinoltCR 321 chromaticity instrument(D65 daylight simulator inside),the color reproduction ratio was calculated with CIE 1976 L*a*b* system. Results: The color reproduction ratio of metal ceramic restoration measured by clinical eye evaluation was 33.9%.Color error ratio was 66.1%. Conclusion: The color reproduction ratio measured by eye evaluation is low and the color error ratio is high.

Extramural vascular invasion(EMVI)was defined as the presence of tumor cells within an endotheliumlined vessel beyond the muscularis propria.High-resolution MRI had 100％sensitivity and 89％specificity in identification of large(caliber greater than 3 mm)EMVI with histopathology as reference.EMVI detected by both histopathology and MRI was significantly associated with local recurrence,distance metastasis and tumor-related death in rectal cancer,which was also identified as an independent prognostic factor for 3-year tumor-free survival and overall survival.EMVI could be used as one of the risk factors,which would allow stratification of patients with rectal cancer to receive neoadjuvant chemoradiotherapy(neoCRT).Moreover,EMVI detected by high-resolution MRI was identified as an independent predictor for response to neoCRT.Tumor regression grading scale defined by high-resolution MRI could be used to evaluate EMVI regression after neoCRT.

Objective To explore the clinical application of two endometrial mechanical stimulation methods in patients with repeated implantation failure (RIF) in frozen-thawed embryo transfer (FET) cycle. Methods A total of 127 women with RIF who planned to perform FET again were included. They were separated into 3 groups: Group A (45 cases) with endometrial scratching and group B (42 cycles) with endometrial sucking within menstrual period in FET cycle, while in control group C (40 cases), no endometrial mechanical stimulation was performed. The outcomes of pregnancy were compared. Results The endometrial types of endometrial scratching group were mainly type A. The embryo implantation rate and clinical pregnancy rate in group A (27.27%, 46.67%) were significantly higher than those in group B (12.75 %, 21.43%) and group C (11.46%, 20.0%) (P <0.05). Conclusion The endometrial mechanical stimulation can improve the endometrial receptivity, compare with endometrial sucking, endometrial scratching can improve the clinical pregnancy rate of RIF patients more obviously.

Objective To compare the status of nurses′cognition on children′s pain between the pediatric intensive care units (PICUs) and common wards .Methods Surveys were completed by the pediatric nurses through the WeChat platform ,3 283 quali‐fied surveys from department of nursing of 11 three level of first‐class hospital in central China ,were sent back and analyzed . Results There was no statistical difference in the age ,marital status ,professional title ,highest academic qualifications ,and the dis‐tribution of professional nursing service life between PICU nurses and common ward nurses(P>0 .05) .Statistical differences were found among the sex ,the positions ,the experiences of nursing and the pediatric nursing (P< 0 .05) .Significant differences were found between the total score of the PNKAS of the PICU nurses and that of common ward nurses(P<0 .05) .Among the four di‐mensionalities ,statistical difference was only found in the item of pharmacological analgesia between PICU nurses and common ward nurses (P<0 .05) .There were no statistical difference on the correct ratio of the top three correct answers and the top three wrong answers between PICU nurses and common ward nurses(P>0 .05) .Conclusion The knowledge of the PICU nurses and common ward nurses were both not optimistic .Specific training should be provided according to the different status of the PICU nurses and common ward nurses ,so that better pain management would be provided to the pediatric children .

BACKGROUND: Bone marrow mesenchymal stem cells (BMSCs) possess the potential in multi-directional differentiation and can differentiate into cardiomyocytes, which had self-discipline jumpiness. There are few reports on intravenous transplantation or autologous mobilization of BMSCs for treating dilated cardiomyopathy-induced heart failure. OBJECTIVE: To study the effect of BMSC intravenous transplantation combined with bone marrow stem cell autologous mobilization on cardiac function in rats with cardiomyopathy-induced heart failure. DESIGN, TIME AND SETTING: This control experiment was conducted at the National Physiological Laboratory, Molecule Laboratory, Animal Experimental Center and Central Laboratory of Shanxi Medical University from January 10th, 2007 to January 10 th, 2008. MATERIALS: Five Wistar rats served as BMSC donor. Fifty rat models of cardiomyopathy-induced heart failure were created by intraperitoneally injected with 15 mg/kg adriamycin for six weeks. Recombinant human granulocyte stimulating factor (GSF) was bought from Qilu Pharmacy Co., Ltd. CD44, CD34 and CD45 were given by Flow Cytometric Analysis Laboratory, Experimental Center, Shanxi Medical University. METHODS: BMSCs were isolated, purified and cultured in vitro. Surface marker CD45, CDD44 and CD34 were detected with f1ow cytometer. Wistar rat models were randomized into 4 groups. BMSCs and recombinant human GSF (50 ?g/kg) were injected into models by tail vein and hypodermoclysis infusion respectively in the combination treatment group (MG group). Recombinant human GSF (50 ?g/kg) was subcutaneously injected into models in the bone marrow stem cell autologous mobilization group (G group). BMSCs were injected into rat models by tail vein in the BMSC group. Rat models in the heart failure group were intact. MAIN OUTCOME MEASURES: ①Peripheral blood leukocyte and mononuclear cell counts and CD34 expression were obtained before treatment and 6 days after treatment. ②At 4 weeks, cardiac function parameters were evaluated by echocardiography and hemodynamic measurement. RESULTS: Compared with that before treatment, peripheral blood leukocyte and mononuclear cell counts significantly increased in the MG group and G group at day 6 (P

0.05). Conclusion The movement of train has not obviously affect to the routine blood test.

Objective To evaluate the clinical value of endoscopic ultrasonography (EUS) in diagnosis and treatment of rectal carcinoid tumom, and the safety and efficacy of endoscopic submucosal dissection (ESD) for rectal carcinoid tumors. Methods All the submucous tumor considered rectal carcinoid tumor received EUS examination and according to the result to select appropriate therapeutic regimen. Results All the 45 lesions were verified rectal carcinoid tumors by pathological examination. 42 patients received endoscopic submucosal dissection. Two tumors invaded into the muscularis propria and one tumor invaded beyond serosa and with lymph node metastasis received surgical strategy. Conclusion EUS can be used to determine surgical strategy for rectal carcinoid tumor for it provides precise information of the size, depth, border, muscularis propria or vessel infiltration of the tumor. For rectal carcinoid tumors smaller than 20 mm in diameter, ESD is safe and effective.