1.Application of UTMD mediated microRNA transfection in disease treatment
Journal of Medical Postgraduates 2017;30(7):780-784
In recent years, research about microRNA has been increasing.How to safely and effectively introduce microRNA into target cells and target tissues is the key to successful treatment of diseases using microRNA.Many studies have reported that ultrasound-targeted microbubble destruction technology (UTMD) mediated gene transfection has the advantages of safety, targeting and high transfection efficiency.The research of UTMD mediated microRNA transfection has also increased year by year.The mechanism of UTMD mediated microRNA transfection and the application in the treatment of diseases such as cancer and cardiovascular system are reviewed in this paper.
2.Experience of Professor JIN Hong-yuan in treating diabetes from seven emotions
China Journal of Traditional Chinese Medicine and Pharmacy 2005;0(05):-
Diabetes is non-infectious chronic disease which is associated with life style,and mental stress is an important pathogenic factor of it.The article explained experience of Professor JIN Hong-yuan in treating diabetes from seven emotions,and approached application of mentation in diabetes from etiopathogenisis,pathogenesis,diagnosis,treatment,health cultivation and so on,at last to elevate effectiveness in clinic.So it had important significance to elevate existence quality and overall-controlled patient's condition through modern psychological support therapy combining with recognition of JIN Hong-yuan on seven emotions.
3.Application of VEGFR in tumor ultrasound molecular imaging
Tianjin Medical Journal 2017;45(9):998-1001
Ultrasound molecular imaging has become one of the hotspots of molecular imaging because of its advantages of non-invasion, non-radiation, repeatability and real-time dynamic imaging. The key of imaging is to select the appropriate target and ligand to bind to the ultrasound contrast agent. The formation of blood vessels in the development of tumor is a significant feature. Vascular endothelial growth factor receptor (VEGFR) is an important specific molecule on tumor vascular endothelial cells, and which can be used as a target for tumor ultrasound imaging. In recent years, many scholars have carried out related studies on the imaging of ultrasound molecules targeting VEGFR. This paper reviewed the mechanism and application of tumor ultrasound molecular imaging with VEGFR as target in recent years.
4.The potential role and significance of tumor necrosis factor-α induced protein 8 like-2 in autoimmune diseases
Medical Journal of Chinese People's Liberation Army 2017;42(8):749-752
Autoimmune disease is a condition arisen from an abnormal immune response to the tissue cells itself, its precise mechanism remains unknown, and the failure to distinguish self from non-self is often termed a breach of tolerance and is the basis for autoimmune illness. The tumor necrosis factor-α (TNF-α) induced protein 8 like-2 (TIPE2) is a newly discovered member of TNF-α induced protein 8 (TNFAIP8) family which is an essential negative controller of both innate and adaptive immunity. It has been documented that marked expressions of TIPE2 are evident in various autoimmune diseases, including autoimmune hepatitis (AIH), primary biliary cirrhosis (PBC), myasthenia gravis (MG) and systemic lupus erythematosus (SLE), which appear to be closely related to the severity, progression as well as prognosis of the illness, thereby contribute to the pathogenesis of autoimmune diseases. Deficient expression of TIPE2 might contribute to the hyper-reactivity of auto-reactive lymphocytes and macrophages, or aggregate inflammatory reaction by prompting high concentration of pro-inflammatory cytokines in peripheral blood, thus, trigger the development and progression of autoimmune diseases. In addition, dysregulation of immune homeostasis could be another latent target involved into the mechanism of autoimmune diseases. The present paper summarized the potential role and its mechanism of TIPE2 in the development of autoimmune diseases.
5.Effect of Tetramethyl Pyrazine on Infarct Areas and Expressions of ED1,IL-1?,TNF-? after Focal Cerebral Ischemia Reperfusion in Rats
Chinese Journal of Information on Traditional Chinese Medicine 2006;0(05):-
Objective To investigate the effect of Tetramethyl Pyrazine(TMP) for treating cerebral infarct by observing infarct areas,and to research its function mechanism by observing the changes of the expressions of ED1,TNF-? and IL-1? in the infarction region after focal cerebral ischemia reperfusion injury.Methods Sixty SD male rats in healthy condition were randomly and averagely divided into normal group,model group and TMP high,middle and low dose group.The cerebral infarct animal model was reproduced by modified thread-tie method.TMP high dose group was administered TMP 140 mg/kg,middle dose group was 120 mg/kg and low dose group was 100 mg/kg by intravenous.Then all the rats were killed.Thirty rats were taken to evaluate whether TMP can decrease the ratio of cerebral infarction areas in the TTC-reacting and formalin fixation brain tissue slices taken from the above-mentioned killed rats,with Pathology Image Analysis System.The masculine of ED1,IL-1? and TNF-? in the immuno-reacting cells in cerebral infarction brain tissue slice taken from other thirty killed rats were observed.Results TMP groups decreased the ratio of cerebral infarction areas,and also decreased ED1,TNF-? and IL-1? in immuno-reacting cell.Conclusion TMP can decrease the formation of cerebral infarction caused by cerebral ischemia reperfusion and its effect is possibly related to the activation of microglia,as well as the immuno-positive expressions of TNF-? and IL-1?.
6.Important role of interleukin-35 in infectious diseases and its significance
Chinese Critical Care Medicine 2017;29(9):848-852
Infectious diseases are resulted from the invasion of an organism's body tissues by multiple disease-causing agents. It has been demonstrated that the occurrence and development of infectious diseases are closely associated with the functional status of immune system. Cytokines play significant roles in modulating the host immune response to the clearance of pathogenic microorganisms and maintaining immune homeostasis. Interleukin-35 (IL-35), as a newly identified member of IL-12 family, exerts suppressive effect on immune response by means of a specific pattern. With the progress of research in recent years, IL-35 might serve as an essential contributor in the immunopathogensis of vast infectious diseases, including hepatitis B, sepsis, tuberculosis and parasite infection, which simultaneously appear to be closely related to the severity, progression as well as prognosis of the illness. Apparently, IL-35 is regarded as a potent and promising anti-inflammatory cytokine in clinical application; its potential value may shed light on the therapeutic strategies for infectious diseases. Herein, we mainly review the potential role and its mechanism of IL-35 in the pathogenesis of infectious diseases.
7.Preliminary study on classified management of surgical doctors surgery permission
Jinhong MA ; Hua YAO ; Lingling FU
Chinese Journal of Hospital Administration 2011;27(8):589-591
According to the related requirements of medical technology clinical application management, the specific implementation approaches of surgery physicians surgery permission management were made, including establishing surgery classification project directory library, developing surgery classification management system and the surgery permission declared, and audit program, and investigating to determine the steps of surgery permission owned by surgery physicians currently, and finding problems and solution measures in implementation surgery permission management. To prevent surgical risk, improve the quality of operation, and evaluate surgical doctors scientifically, it provides management experience and references.
8.Expressions and clinical significances of TK1 and Ki-67 in triple negative breast cancer
Yuchao NIU ; Yuan YAO ; Xuezhen MA
Journal of International Oncology 2016;43(6):409-413
Objective To analyze the expressions of thymidine kinase 1 (TK1) and nuclear-associated antigen Ki-67 in triple negative breast cancer (TNBC) and their clinical significances.Methods One hundred and twenty tumor tissue sections of patients with breast cancer who were performed breast conservation treatment or modified mastectomy in the Second Affiliated Hospital of Qingdao University Medical College from June 2009 to December 2010 were collected,and there were 60 cases with TNBC and 60 cases with non-TNBC.The expressions of TK1 and Ki-67 in different breast tissues were detected by immunohistochemistry.The relationships between the expression status and clinicopathologic features were analyzed.Results The positive expression rates of TK1 in TNBC and non-TNBC were 83.33% and 51.67% respectively,with a significant difference (x2 =13.713,P =0.000).The positive expression rates of Ki-67 expression in TNBC and nonTNBC were 68.33% and 31.67% respectively,with a significant difference (x2=16.133,P =0.000).In TNBC,the expression of TK1 was related to histological staging (x2 =6.125,P =0.013),but it was not related to onset age (x2 =0.809,P =0.369),menopausal stutas (x2 =1.615,P =0.204),tumor size (x2 =0.054,P =0.816) and lymphatic metastasis (x2 =0.672,P =0.412).In TNBC,the expression of Ki-67 was related to histological staging (x2 =13.145,P =0.000) and lymphatic metastasis (x2 =6.182,P =0.013),but it was not related to menopausal stutas (x2 =1.018,P =0.313),onset age (x2 =2.377,P =0.123) and tumor size (x2 =2.401,P =0.121).The expression of TK1 was positively correlated with that of Ki-67 (r =0.369,P =0.023).The results of survival analysis showed that the disease-free survival rates of 5-year were 28.20% and 66.70% in the TK1 positive group and TK1 negative group,and the disease-free survival rates of 5-year were 24.30% and 64.30% in the Ki-67 positive group and Ki-67 negative group,with significant differences (x2 =4.194,P=0.041;x2 =4.540,P =0.033).Conclusion TK1 and Ki-67 are highly expressed in TNBC,and their expressions are correlated with histological staging and survival,which are expected to become prognostic indicators.
9.A DATABASE SOFTWARE BEING USED TO MONITOR DIABETIC RETINOPATHY
Junping ZHAO ; Yi YAO ; Zhizhong MA
Medical Journal of Chinese People's Liberation Army 1983;0(05):-
The backward condition of clinical and fundamental investigation of diabetic retinopathy was unfit for the fast increasing diabetic patients and the high incidence of diabetic retinopathy. A database software of diabetic retinopathy, was developed. The system using windows 9x/NT has six functions as follows: patients′ general situation, examination table, checking picture index and users′ administration. The characteristics of the sole criterion, accuracy, continuity, completeness, frequency, different comparison, annexing the functions of computer based patient record which are profitable for long distance consultation and training.
10.Treatment of female bladder neck obstruction with transurethral resection of bladder neck: Report of 26 cases
Yingzhong LI ; Chaolong MA ; Zhengyou YAO
Chinese Journal of Minimally Invasive Surgery 2001;0(03):-
15 ml/s;dysuria was significantly improved in the other 2 cases,with the Qmax being 13 and 11 ml/s,respectively.Conclusions Transurethral resection of bladder neck is an effective means for the treatment of female bladder neck obstruction.