Objective To assess the efficacy and safety of recombinant human Ad-p53( rAd-p53) injection combined with cisplatin in treating malignant pleural effusion by conducting a Meta-analysis. Methods A comprehensive collection of randomized controlled studies(RCTs)of rAd-p53 combined with cisplatin versus single cisplatin in the treatment of malignant pleural effusion was retrieved form Cochrane Library,Pubmed, EMBase,CBM,CNKI,Wanfang and VIP databases. Meta-analysis was conducted by RevMan 5. 2 software. Results Ten RCTs involving 538 patients were identified in the study. The Meta-analysis results showed that rAd-p53 combined with cisplatin group had a higher hydrothorax total remission rate than single cisplatin group (RR = 1. 67,95% CI:0. 90-2. 01;Z = 5. 51,P = 0. 000 01). The PS score in rAd-p53 combined with cisplatin group was better than that in single cisplatin group(RR = 1. 76,95% CI:1. 45-2. 14;Z = 5. 69,P <0. 000 01). The incidence rate of adverse reaction of heating in the former was higher than that in the latter (RR = 3. 10,95% CI:2. 25-4. 27;Z = 6. 94,P < 0. 000 01). There were no significant differences in adverse reactions of chest pain(RR = 0. 99,95% CI:0. 50-1. 96;Z = 0. 04,P = 0. 97),gastrointestinal reaction (RR = 1. 16,95% CI:0. 90-1. 50;Z = 1. 13,P = 0. 26)and marrow suppression(RR = 1. 09,95% CI:0. 81-1. 47;Z = 0. 59,P = 0. 55)between the two groups. Conclusion rAd-p53 combined with cisplatin therapy for the treatment of malignant pleural effusion is superior to single cisplatin therapy. Mean while,fever is self-limited,and the safety is identified. It is worthy for promotion of rAd-p53 injection combined with cisplatin inclinical treatment of advanced malignant pleural effusion.

Objective:To investigate the effect of combination of D-methionine and docetaxel on gastric cancer cells. Methods:First, seven gastric cancer cell lines were cultured with three different kinds of media (D-Met, L-Met and Met~ - ) for five days, and cell cycle was detected by flow cytometry. Second, gastric cancer cells were respectively cultured in L-Met, D-Met, and Met~ - media for 96 h, then docetaxel was added into 3 media. After 24 h, proliferation was measured by MTT method. Results:All gastric cancer cell lines were arrested in G_ 2 /M phase (P

Objectives: To study the effect of D-methionine on gastric cancer and its mechanism. Methods: We used a medium with D-methionine to culture six gastric cancer cell lines. The medium with L-methionine acted as control to culture cells. The proliferation of cells was detected by MTT. Apoptotic rates and cell cycle were detected by FMC. Results: Absorbance of all cell lines was significantly lower than control (P0.05). KATO-Ⅲ cells stayed more in G 0 /G 1 phase (P

Objective: To understand the changing trend of the teaching environment of the elementary and secondary schools in Zhengzhou, and to provide data for further improving the teaching environment of the elementary and secondary schools. Methods: A survey of teaching facilities and teaching aids in 534 secondary and primary schools in Zhengzhou was conducted from 2014 to 2018 by using a stratified random sampling method. Results: Teaching facilities: the qualified rate of the railings in the outer corridor of the teaching building was 88.8% (474), the qualified rate of classroom lighting was 79.6% (425), the qualified rate of the light desk distance was 82.8% (442), and the qualified rate of black plate lights 86.1% (460), the qualified rate of each year was statistically significant ( χ 2=9.97, 23.74, 80.10, 53.33, P <0.05); the qualified rate of classroom area per capita was 52.8% (282), the qualified rate of the outer corridor width of the teaching building was 64.0% (342), the qualified rate of each year showed no statistical difference( χ 2=7.38, 8.71, P >0.05). Teaching ancillary facilities: the proportion of centralized water supply was 65.0% (347), toilet with hand washing facilities was 81.6% (436), these two indicators showed an increasing trend, hand washing facilities in campus was 70.6% (377), showed a decreasing trend, the rate of each year differed significantly( χ 2=88.62, 42.71 , 123.53, P <0.05). Conclusion The overall situation of teaching environment in Zhengzhou primary and secondary schools is relatively good, the qualified rate of most indicators of teaching facilities increased from 2014 to 2018. However, there are still some indicators to be strengthened.

Objective To investigate the effects of thermosensitive moxibustion on psoriasis model rats'skin;To discuss its mechanism based on JAK3/STAT3 signaling pathway.Methods 5%imiquimod cream was used on the skin of rats back and along the route of the Sanjiao meridian of hand-shaoyang for psoriasis modeling.The SD rats were randomly divided into blank group,model group and treaament group.The treatment group received moxibustion therapy at"Waiguan"for 40 minutes each time for 7 consecutive days,and were divided into moxibustion group and thermosensitive moxibustion group according to sensitization state.HE staining was used to observe pathological changes in skin lesions,and ELISA was used to detect serum tumor necrosis factor(TNF)-α,interleukin(IL)-1 and IL-23 levels.Immunohistochemistry was used to detect the positive expressions of p-JAK3 and p-STAT3 in the skin lesion tissue,and RT-qPCR was used to detect the mRNA expressions of JAK3,STAT3,IL-17A and IL-17F in the skin lesion tissue.Results Compared with the normal group,model group rats were with protruding and thickened skin lesions accompanied by extensive infiltration of inflammatory cells,the contents of serum TNF-α,IL-1β and IL-23 significantly increased(P<0.01),and the positive expressions of p-JAK3 and p-STAT3 in the skin lesion tissue significantly increased(P<0.01),the mRNA expressions of JAK3,STAT3,IL-17A and IL-17F significantly increased(P<0.01).Compared with the model group,the thickness of the spinous layer in the skin lesions of rats in the thermosensitive moxibustion group was reduced,and there was no significant infiltration of inflammatory cells.The contents of serum TNF-α,IL-1β and IL-23 significantly decreased(P<0.01),and the positive expressions of p-JAK3 and p-STAT3 in skin lesion tissue significantly decreased(P<0.05,P<0.01),the mRNA expressions of JAK3,STAT3,IL-17A and IL-17F significantly decreased(P<0.05,P<0.01).Compared with the moxibustion group,the serum contents of TNF-α and IL-1β in the thermosensitive moxibustion group were significantly decreased(P<0.05,P<0.01),and the positive expression of p-JAK3 in the skin lesion tissue was significantly decreased(P<0.05).Conclusion Thermosensitive moxibustion can improve the pathological symptoms of psoriasis model rats.Its mechanism may be related to the inhibition of the JAK3/STAT3 signaling pathway,reduction of regulatory T cell-17 differentiation and secretion of pro-inflammatory cytokines.