1.Influence rules of reinforced materials on the properties of shape memory polymer
Haipo CUI ; Mengxue ZHANG ; Yanzhe YOU
International Journal of Biomedical Engineering 2016;39(1):43-48
Shape memory polymer (SMP) is a new type of functional materials.SMP has lots of advantages such as low density,light weight,high shape-recovery rate and low cost,but its stiffness is low and shape recovery force is small.Therefore,the reinforced SMP composite has become a hot research focus.The latest progress of reinforced SMP composite is reviewed,with the emphasis placed on the analysis of the influence of reinforced materials on SMP properties including short fibers,particles and the mixture of fibers and particles.Finally,problems exist in the study and the prospect of reinforced SMP composite are discussed briefly.
2.Study on bone metobolism change in thirty hyperthyroidism patients
Huanqi GE ; Hanqing CAI ; Xiujuan ZHANG ; Yanzhe ZHANG ; Xuejun HUI
Journal of Jilin University(Medicine Edition) 2000;26(6):612-614
Objective:To study the effect of thyroid function on bone metabolism. Methods:Serum FT3,FT4 were investigated by radioimmunoassay (RIA) and bone mineral density of spine (L2~4) weremeasured by dual energy x-ray absorptinmetry and other markers related to bone metabolism were alsomonitored in 30 patients with hyperthyroidism and 30 healthy volunteers. Results :The levels of FT3,FT4,ALP were significantly higher than those of the normal controls. BMD of spine decreased significantly incomparison with the controls ,and the degree of severity and incidence increased with age. Conclusion:Thy-roid hormone might speed up bone turnover directly with increased bone resorption to induce bone massloss.
3.Biomechanical analysis of two fixation methods in treating spine fracture and dislocation
Chengdong PIAO ; Yanzhe ZHANG ; Lei CHEN ; Hongshun MA
Chinese Journal of Tissue Engineering Research 2009;13(52):10279-10282
BACKGROUND:Thoracic or lumbar spine fracture and dislocation mainly treated by internal/external fixation devices with surgical methods.Fixation methods in treating spine fracture and dislocation is an important subject for scholars.OBJECTIVE:To quantitative analyze the treatment of spinal fracture and dislocation using pedicle screw and plate fixation,and to provide mechanical parameters for clinical application.METHODS:Shimadzu electronic universal testing machine was used to simulate L_1 lumbar fracture and dislocation treated by plate fixation and pedicle screw fixations,specimens were underwent flexion,extension,compression,as well as torsion tests,with speed of 5 mm/min.The torsion test was performed on the torsion machine with speed of 0.05 (°) / s.All the experimental data were analyzed by statistical analysis and paired t test.RESULTS AND CONCLUSION:The results demonstrated that the outcomes of compression test had no significant differences between 2 groups (P>0.05).The left and right torsion angle of the pedicle screw fixation group was smaller than that of the plate fixation group (P<0.05),and the flexion and extension displacements was smaller in the pedicle screw fixation group than in the plate fixation group (P<0.05).It suggested that plate fixation is worse than pedicle screw fixation,while pedicle screw fixation is conductive to bone healing,thus,pedicle screw fixation is a better internal fixation device.
4.cDNA cloning and sequence analysis of ribosomal protein S7 gene from giant panda
Yiling HOU ; Chunlian WU ; Wanru HOU ; Yanzhe HAO ; Tian ZHANG
Journal of Third Military Medical University 2003;0(18):-
Objective To explore the structure of ribosomal protein S7 ( RPS7) gene of giant panda ( Ailuropoda melanoleuca) and investigate its homologies with other already reported sequences,including Homo sapiens,Mus musculus,Rattus norvegicus and Bos taurus. Methods The cDNA of RPS7 was cloned from the giant panda by RT-PCR. The sequence data were analyzed by GenScan software. Blast 2. 1 was used to study the homology of the obtained RPS7 sequence with the gene sequences of other species; Open reading frame ( ORF) of the DNA sequence was searched using ORF finder software; Protein structure of the RPS7 sequence cloned was deduced using Predict Protein software. Results The full length of the sequence fragment was 589 bp containing an ORF of 585 bp. The deduced protein sequence showed that the protein was composed of 194 ami- no acids and its estimated molecular weight was 22. 126 85 ?103 with a pI of 10. 09. There were 7 different pat- terns of functional sites: one N-glycosylation site; two cAMP and cGMP-dependent kinase phosphorylation sites; four casein kinase C phosphorylation sites; one casein kinase Ⅱ phosphorylation site; two N-myristoylation sites; two amidation sites and one ribosomal protein S7e signature site in the RPS7 protein. Further analysis indicated that the sequence of RPS7 and the protein encoded were highly homologous to some mammals reported.Conclusion The complete coding sequence of RPS7 gene has been cloned through RT-PCR successfully, which is the first report on the RPS7 gene from the giant panda.
5.The Effect of D-methionine on Gastric Cancer
Jihong LIU ; Weixin CAO ; Yubao JI ; Yi ZHANG ; Bingya LIU ; Zhenggang ZHU ; Min YAN ; Yanzhe LIN
Parenteral & Enteral Nutrition 1997;0(01):-
Objectives: To study the effect of D-methionine on gastric cancer and its mechanism. Methods: We used a medium with D-methionine to culture six gastric cancer cell lines. The medium with L-methionine acted as control to culture cells. The proliferation of cells was detected by MTT. Apoptotic rates and cell cycle were detected by FMC. Results: Absorbance of all cell lines was significantly lower than control (P0.05). KATO-Ⅲ cells stayed more in G 0 /G 1 phase (P
6.Establishment of rapid influenza virus detection technology based on an integrated system
Xiaoshan ZHANG ; Jing MA ; Yanzhe HAO ; Jing YANG ; Meiling HOU ; Hongxia LI ; Jingduan LIU ; Haodi MENG ; Yuxi CAO ; Xiaoguang ZHANG
Chinese Journal of Experimental and Clinical Virology 2024;38(3):319-325
Objective:This study aimed to develop a rapid and accurate integrated nucleic acid detection method tailored for the influenza virus.Methods:We designed primers and probes targeting the predominant influenza virus strains circulating in China in recent years. These were integrated with extraction and amplification reagents and a point of care testing (POCT) system to facilitate a seamless and expedited process involving nucleic acid extraction, reaction system preparation, amplification, and result interpretation for the influenza virus. The specificity of the POCT system was evaluated using cultured influenza viruses, while its cross-reactivity was assessed against common respiratory pathogens, including adenovirus and respiratory syncytial virus.Results:Our study successfully developed duplex amplification primers and probes for both influenza A and B viruses, achieving a detection threshold as low as 500 copies/ml. Specificity tests confirmed that the detection reagents did not show cross-reactivity with other respiratory pathogens such as adenovirus and respiratory syncytial virus. The POCT-based rapid nucleic acid detection method for influenza virus was established, it is capable of completing the entire process from nucleic acid extraction to amplification and result interpretation within 50 minutes, while enabling real-time data upload.Conclusions:The POCT-based rapid influenza virus detection kit developed in this study offers a " sample in, results out" convenience, making it suitable for rapid influenza virus detection in primary care settings. This innovation has significant potential for clinical application.
7.Development of an Integrated Disposable Device for SARS-CoV-2 Nucleic Acid Extraction and Detection
Ma JING ; Hao YANZHE ; Hou MEILING ; Zhang XIAOSHAN ; Liu JINGDUAN ; Meng HAODI ; Chang JIANGBO ; Ma XUEJUN ; Liu JIHUA ; Ying QINGJIE ; Wang XIANHUA ; Li HONGXIA ; Cao YUXI ; Zhang XIAOGUANG
Biomedical and Environmental Sciences 2024;37(6):639-646
Objective To develop a highly sensitive and rapid nucleic acid detection method for the severe acute respiratory syndrome coronavirus 2(SARS-CoV-2). Methods We designed,developed,and manufactured an integrated disposable device for SARS-CoV-2 nucleic acid extraction and detection.The precision of the liquid transfer and temperature control was tested.A comparison between our device and a commercial kit for SARS-Cov-2 nucleic acid extraction was performed using real-time fluorescence reverse transcription polymerase chain reaction(RT-PCR).The entire process,from SARS-CoV-2 nucleic acid extraction to amplification,was evaluated. Results The precision of the syringe transfer volume was 19.2±1.9 μL(set value was 20),32.2±1.6(set value was 30),and 57.2±3.5(set value was 60).Temperature control in the amplification tube was measured at 60.0±0.0 ℃(set value was 60)and 95.1±0.2 ℃(set value was 95)respectively.SARS-Cov-2 nucleic acid extraction yield through the device was 7.10×106 copies/mL,while a commercial kit yielded 2.98×106 copies/mL.The mean time to complete the entire assay,from SARS-CoV-2 nucleic acid extraction to amplification detection,was 36 min and 45 s.The detection limit for SARS-CoV-2 nucleic acid was 250 copies/mL. Conclusion The integrated disposable devices may be used for SARS-CoV-2 Point-of-Care test(POCT).
8.Theoretical models for influenza vaccination behavior at the individual level
Kai QU ; Yulu MIAO ; Simeng FAN ; Yanzhe LIU ; Xiaokun YANG ; Hongting ZHAO ; Ying QIN ; Jiandong ZHENG ; Yanping ZHANG ; Zhibin PENG ; Zijian FENG
Chinese Journal of Epidemiology 2024;45(4):608-614
Influenza imposes a significant disease burden on society and individuals annually, and influenza vaccination is considered a significant public health measure to prevent influenza and reduce influenza-related severe disease and death. The low influenza vaccination rate in China is partly due to certain factors affecting the willingness and behavior of individuals to receive them. Scientific research and targeted interventions on these factors can effectively improve the vaccination situation. Commonly used individual-level theoretical models for influenza vaccination behavior include the health belief model, protection motivation theory, and theory of planned behavior. This study reviews theoretical models commonly employed in researching influenza vaccination willingness and behavior. An overview of these practical applications and challenges models is presented to provide references for relevant research and intervention programs in China.
9.Construction and Thinking of Data Science System of Chronic Atrophic Gastritis
Jianhui SUN ; Weichao XU ; Xia ZHANG ; Runxue SUN ; Yanzhe CHEN ; Shaopo WANG ; Yuman WANG ; Zhen LIU ; Yanru DU ; Qian YANG ; Jianming JIANG
Journal of Traditional Chinese Medicine 2024;65(12):1208-1212
Taking chronic atrophic gastritis (CAG) as an example, the frontier technologies in data science have been introduced into the inheritance, innovation and development of traditional Chinese medicine (TCM), providing reference for conducting real-world clinical research on specialized diseases of TCM. This paper put forward the construction of CAG data science system by elaborating the connotation of data science and its application value in TCM, and discussed the path to build CAG data science system, namely through "data acquisition-knowledge expression-knowledge reasoning" to establish CAG database, knowledge base and develop diagnosis platform differentiating diseases and syndromes. Besides, this paper analyzed the prospects of CAG data science in improving data governance ability and knowledge discovery efficiency, deepening the level of knowledge sharing, promoting interdisciplinary integration, and strengthening the integration process of industry, academia and research.
10. Clinical analysis of 103 children with Angelman syndrome
Jinmei SHEN ; Yuanfeng ZHOU ; Xiaonan DU ; Shasha LONG ; Xiaodong LU ; Yanzhe ZHANG ; Liwen YANG ; Yalan DOU ; Weili YAN ; Yi WANG
Chinese Journal of Applied Clinical Pediatrics 2019;34(12):911-914
Objective:
To investigate the correlation between clinical phenotype, electroencephalogram (EEG) characteristics and genotype in children with Angelman syndrome(AS).
Methods:
A total of 103 children with AS at Department of Neurology, Children′s Hospital of Fudan University from June 2017 to June 2018, were included in this study.The information of clinical characteristics, EEG manifestations, genotypes as well as the epileptic outcome were collected retrospectively.The correlations between clinical phenotype, genotype, and epileptic outcome were evaluated.
Results:
(1) Of the 103 cases, 48 were male (46.6%) and 55 were female (53.4%). (2) Genotypes on AS critical region were maternal chromosome 15q11.2-q13 [86.4%(89/103 cases)], paternal uniparental disomy [3.9%(4/103 cases)], imprinting defects [1.9%(2/103 cases)], and mutations in the maternal copy of