Objective To explore the anti- inflammatory of pricking blood therapy in acute gouty arthritis rats. Methods 60 Sprague-Dawley rats were equally divided into normal group, bloodletting normal group, sham group, arthritis group, bloodletting group and ibuprofen group. The acute gouty arthritis model was established with injecting uric acid sodium salt into the right ankle joint cavity. The ibuprofen group was administrated with ibuprofen intragastrically, the bloodletting normal group and bloodletting group were pricked the right Kunlun (BL60) acupoint. The cross section diameter of the right ankle joint were measured before and after treatment. Levels of mRNA and protein of interleukin (IL)-10 and IL-4 expressed in ankle were determined with RT-PCR and Western blotting Results The cross section diameter increased in the bloodletting group compared with the normal group after treatment (P<0.05), and decreased (P<0.05) compared with the arthritis group and the ibuprofen group, while the expression of IL-10 mRNA increased (P<0.05) compared with the normal group, the arthritis group and the ibuprofen group, as well as the IL-10 protein compared with the normal group and the arthritis group (P< 0.05). The expression of IL-4 mRNA and protein increased without significance (P>0.05) in the bloodletting group compared with the normal group. Conclusion IL-10 may play a role of anti-inflammatory in pricking bloodletting therapy for acute gouty arthritis.

Objective To investigate illness perceptions of primary nocturnal enuresis(PNE) children's parents,to summarize the nursing countermeasures.Methods A total of 232 children's parents were evaluated using PNE knowledge questionnaire from November 2011 to November 2014.According to the results,children and parents were cared and educated targetly.Then parents were evaluated again after the treatment and nursing intervention.Results A total of 210 parents completed the evaluation,95.2％ parents knew PNE was a disease,90.0％ parents had correct knowledge of PNE,91.9％ parents clearly knew the inducing factors of PNE,91.0％ parents clearly knew the detriment of PNE,89.5％ parents clearly knew the treatment method of PNE,94.8％ parents knew the recovery of PNE.There were significant differences before and after the nursing intervention (x2 =235.347,215.872,207.239,196.039,207.499,216.463,respectively;P ＜ 0.05).Conclusions Newly diagnosed PNE children's parents have a little awareness of the disease,especially lack of the awareness of inducing factors and treatment methods.According to personalcharacter,nursing education can improve the awareness of the disease,and enhance the level of disease control so as to reduce the economic burden on family and society.