OBJECTIVE To investigate the effect of Guanxinning tablets(GXN) on early heart failure model rats, and to explore the protective mechanism of GXN on heart failure rats from the perspective of intestinal flora. METHODS Six rats who underwent sham operation were set as sham operation group. Took 80 SD rats to undergo aortic arch stenosis and established a heart failure rat model. The surviving rats were divided into 4 groups, namely the model control group, the positive control group(captopril tablets 12.5 mg·kg–1), high-dose and low-dose of GXN group(600, 1 200 mg·kg–1). The 4 groups were administered continuously for 8 weeks. Cardiac ultrasonography was performed every 4 week. Serum NT-proBNP, hs-CRP, IL-6, TNF-α, SOD and MDA levels were measured. The effects of GXN on the structure and function of intestinal flora were observed based on the high-throughput sequencing technology and bioinformatics analysis of 16S gut microbiome. RESULTS Compared to the model control group, after giving different doses of GXN, the survival rate of rats increased, and the thickness of the ventricular wall decreased to varying degrees. The weight of the heart and coefficient of the heart were all reduced. GXN could also reduce the level of inflammatory factors, inhibit the level increase of NT-proBNP in rats, and increase the activity of serum SOD. In addition, GXN intervention could significantly improve the intestinal flora diversity of rats with heart failure, the possible target genera of GXN were Akkermansia genera, Phascolarctobacterium genera and Oxalobacter genera. The effect of GXN on intestinal function in rats with heart failure might be concentrated in non-homologous end-joining, influenza A, carotenoid synthesis, indole alkaloids biosynthesis, betalain biosynthesis, renin-angiotensin system and other biological pathways. CONCLUSION The protective effect of GXN on early heart failure rats may be related to the regulation of intestinal flora pathway.

ObjectiveBased on the hemodynamic indicators of transcranial Doppler （TCD），this study aimed to analyze the correlations of visual analogue scale（VAS） and Hamilton depression rating scale 24-item（HAMD-24） scores with mean blood velocity （Vm） of the anterior cerebral artery （ACA），the middle cerebral artery （MCA）， and the posterior cerebral artery （PCA），and the correlation between VAS score of headache and HAMD-24 score，and explore the clinical efficacy of Xiaoyaosan combined with paroxetine tablets in the treatment of migraine with liver depression and blood deficiency syndrome and depression. MethodSixty-two outpatients treated in the encephalopathy department， the First Afffiliated Hospital of Anhui University of Chinese Medicine between January 2019 to June 2020 who were in line with the diagnostic criteria were enrolled and divided into a treatment group （32 cases） and a control group （30 cases）. All patients were treated with paroxetine tablets，while those in the treatment group received additional Xiaoyaosan. The changes in relevant indicators in the two groups before and after treatment were observed. The effects of Xiaoyaosan on the TCD indicators （Vm_ACA， Vm_MCA， and Vm_PCA）， scores of migraine and depression ［including VAS score， Headache Impact Test-6 （HIT-6） score，and HAMD-24 score］， and the traditional Chinese medicine （TCM） syndrome score were analyzed. ResultVAS and HAMD-24 scores were positively correlated with Vm_ACA， Vm_MCA， and Vm_PCA （P<0.01）. VAS scores were positively correlated with HAMD-24 scores. Compared with the conditions before treatment， the levels of TCD indicators （Vm_ACA， Vm_MCA， and Vm_PCA） in the two groups decreased after treatment （P<0.01）， and the scores of HIT-6， VAS， and HAMD-24 in the two groups were significantly improved （P<0.01）. Compared with the control group after treatment， the treatment group showed decreased levels of TCD indicators （Vm_ACA， Vm_MCA， and Vm_PCA） （P<0.01） and superior improvement of HIT-6， VAS， and HAMD-24 scores （P<0.01）. The effective rate in the treatment group was 90.6% （29/32）， higher than 63.3% （19/30） in the control group （χ²=5.13，P<0.05）. There was no significant difference in adverse reactions between the two groups. ConclusionTCD indicators can effectively reflect the cerebral blood flow of patients with migraine and depression co-morbidity，and migraine is correlated with the onset and severity of depression. The comprehensive analysis showed that the efficacy of Xiaoyaosan combine paroxetine tablets was better than that of paroxetine tablets alone in the treatment of migraine with liver depression and blood deficiency syndrome and depression. This therapeutic protocol can significantly improve cerebral blood flow and the symptoms and degree of migraine and depression and can provide references for clinical treatment.

Objective:To explore the role of transgelin(TAGLN) in the occurrence and development of papillary thyroid carcinoma (PTC) and its possible signal pathway.Methods:One hundred cases of PTC tissues and corresponding paracancerous normal thyroid tissues were collected. Realtime quantitative PCR (RT-qPCR), Western blotting, and immunohistochemistry were used to analyze the expression of TAGLN in PTC tissues and corresponding paracancerous normal thyroid tissues. PTC cells were transfected with plasmid and shRNA lentivirus vector respectively to up-regulate or down-regulate the expression of TAGLN in order to detect the effects of them on the proliferation, invasion, and migration by cell proliferation assay(cell counting kit-8, CCK-8)and cell invasion and migration assays (Transwell). The effects of TAGLN on mitogen-activated protein kinase (MAPK)/extracellular-signal regulating kinase (ERK) signal pathway was detected with Western blotting.Results:RT-qPCR showed that there was no difference in the expression of TAGLN mRNA between PTC and corresponding paracancerous normal thyroid tissues ( P>0.05); Western blotting demonstrated that the expression of TAGLN protein in PTC tissues was significantly lower than that in corresponding paracancerous normal thyroid tissues ( P<0.01). Immunohistochemical results revealed that the expression of TAGLN in PTC tissues was significantly lower than that in corresponding paracancerous normal thyroid tissues. Overexpression of TAGLN inhibited the proliferation, invasion, and migration of PTC cells ( P<0.01), but knockdown of TAGLN promoted the proliferation, invasion, and migration of PTC cells ( P<0.01). Overexpression of TAGLN decreased the expression of phosphorylated ERK ( P<0.05), whereas silencing TAGLN increased phosphorylated ERK level in PTC cells( P<0.01). Conclusion:The expression of TAGLN in PTC is significantly decreased. It is related to the occurrence and development of PTC, and its mechanism may be related to MAPK/ERK signal pathway.

Objective:To explore the application effect of patient mutual assistance model guided by the hierarchy of needs theory in patients with acute myocardial infarction.Methods:Using the convenient sampling method, 40 patients with acute myocardial infarction in the Fourth People's Hospital of Haikou from September 2018 to October 2019 were selected as the control group and 40 patients with acute myocardial infarction from November 2019 to January 2021 were selected as the intervention group. The control group implemented the conventional nursing model, and the intervention group implemented the patient mutual assistance model guided by the hierarchy of needs theory on this basis. Perceived Stress Scale (PSS) , SDS and Herth Hope Scale (HHI) were used to evaluate the effect of intervention.Results:After the intervention, the total scores of PSS of patients in the two groups were lower than those before the intervention, and the difference was statistically significant ( P<0.01) . And the total PSS score of the intervention group was lower than that of the control group, and the difference was statistically significant ( P<0.01) . After the intervention, the total SDS score of patients of the two groups was lower than that before the intervention, and the difference was statistically significant ( P<0.01) . And the total SDS score of the intervention group was lower than that of the control group, and the difference was statistically significant ( P<0.01) . After intervention, the scores of HHI dimensions and total scores in two groups were higher than before intervention, and the differences were statistically significant ( P<0.01) . The scores of HHI dimensions and the total score of the intervention group were higher than those in the control group, and the differences were statistically significant ( P<0.01) . Conclusions:The patient mutual assistance model guided by the hierarchy of needs theory can effectively reduce the self-perceived pressure of patients with acute myocardial infarction after PCI, improve their depression and increase the level of hope.

Objective: To explore an efficient method for the establishment of 3D finite element model based on CBCT images. Methods: Mandible of a male volunteer was scanned by CBCT, and the resulting DICOM data was used for 3D reconstruction in Mimics17 software. Then with the. stl format file, the result of 3D reconstruction was imported into Geomagic Warp 2015, in which 3D models consisting of triangular patches for dentition, periodontal ligament and alveolar bone were created. With free meshing algorithm, the 3D finite element model of mandible with full dentition consisting of 10-node tetrahedron elements was obtained under the constraint that the maximum inner angle was set to be 25°. Results: The 3D finite element model for human mandible with full dentition was established. The total number of nodes is 299286, the elements number for dentition, periodontal ligament and alveolar bone are105805, 122427 and 577529, respectively. Conclusion: The proposed method can be used for the establishment of 3D finite element model of mandible with full dentition based on CBCT images, and it has the merits of good stability, high precision and wide application compared with the traditional modeling method.

Objective: To study the influence of two-wall bone defect on periodontal ligament stresses under normal occlusal forces, and to analyze the influence of depth and width of bone defect to periodontal ligament stresses. Methods: Three-dimensional finite element models of teeth, periodontal ligament and alveolar bone were created based on cone beam CT images. Proximal two-wall bone defect with different depths (bone defect occupies one third, two thirds, and full length of root) and widths (bone defect occupies one fourth, two fourths, three fourths and full width of buccal lingual width) were simulated by modifying the elastic modulus of elements within defect areas. Occlusal forces with magnitudes of half of the maximum occlusal forces were applied to the model at an angle of 45° to the long axis of tooth, and equivalent stresses of periodontal ligament were analyzed. Results: In the model of no bone defect, the equivalent stresses of periodontal ligament of incisors, canines, premolars and molars were 2.88, 2.31, 8.67 and 7.53 MPa respectively. The equivalent stresses of periodontal ligament increased with the enlargement of depth and width of bone defect. The equivalent stresses of periodontal ligament with maximum bone defect in both depth and width for incisors, canines, premolars and molars were 4.47, 3.62, 11.66 and 8.72 MPa respectively. In the model of width of bone defect was consistent and bone defect develops vertically, the increments of equivalent stresses of periodontal ligament were significantly greater in the early stage bone defect model (from no defect to one third of root length bone defect) than that in the later stage bone defect model (from two thirds to full length of root length bone defect). In the model of bone defect depth was consistent and bone defect develops transversely, the increments of equivalent stresses of periodontal ligament in the early stage bone defect model (from no defect to one fourth of buccal lingual width bone defect) were significantly smaller than that in the later stage bone defect model (from three fourths to full width of buccal lingual width bone defect). Conclusions Bone defect with shallow depth and that with large width would increase periodontal ligament stresses. Therefore, more attention should be paid to occlusion evaluation and adjustment for teeth with shallow and wide two-wall bone defect.

Objective To explore the detection trend of vaginal intraepithelial neoplasia(VaIN)of lower genital tract from 2013 to 2015. Methods A retrospective analysis was undertaken of colposcopy-directed biopsy of cervical, vaginal and vulvar intraepithelial neoplasia lesions include cervical intraepithelial neoplasia (CIN), VaIN and vulvar intraepithelial neoplasia (VIN) in Obstetrics and Gynecology Hospital of Fudan University from January 2013 to December 2015. Results (1) Overall data of CIN, VaIN and VIN:a total of 16732 cases were diagnosed of lower genital intraepithelial neoplasia in 3 years, accounting for 23.20% (16732/72128) of total colposcopy-directed biopsy cases. Among them, CIN, VaIN and VIN accounted for 19.48%(14053/72128), 2.67%(1923/72128), 1.05%(756/72128) of total colposcopy-directed biopsy cases of the lower genital tract, 83.99%(14053/16732), 11.49%(1923/16732), 4.52%(756/16732) of total lower genital intraepithelial neoplasia, respectively. (2) Annual data of CIN, VaIN and VIN from 2013 to 2015. The annual proportion of CIN in all intraepithelial neoplasia of lower gential tract was basically stable, consisting of 86.02%(3955/4598),83.25%(4795/5760) and 83.20%(5303/6374), respectively. The annual proportion of VaIN was gradually increasing, consisting of 8.09% (372/4598), 12.45%(717/5760) and 13.08%(834/6374), respectively. The annual proportion of VIN was gradually decreasing, consisting of 5.89% (271/4598), 4.31% (248/5760) and 3.72% (237/6374), respectively. Conclusion The increasing detection of VaIN from 2013 to 2015 might correlate with the increasing attention to inspection of the entire vaginal wall.

Objective To observe the post-laparoscopic analgesic effect of oxycodone combined with dexmedetomidine for gastric cancer patients.Methods A total of 40 gastric cancer patients who underwent laparoscopy during November 2016 and January 2017 and assessed with physical status Ⅰ or Ⅱ according to American Society of Anesthesiologists,were randomly divided into 2 groups:oxycodone group (group O,n =20) and oxycodone plus dexmedetomidine group (group OD,n =20).The anestheasic effects at the time points of hours 2,5,8,11 and 24 after the operation were assessed by using visual analog pain score,times for pressing the pump,Ramsay score and patient's satisfaction index of the two groups.At the same time,the adverse effects of two groups were recorded and observed.Results The analgesic effect after starting the pump at hours 2,5,8,11,24 and 25,the VAS scores and the pressure time in group OD were significantly lower than in group O (P ＜ 0.05),and the Ramsay scores were insignificantly different between the groups (P ＞ 0.05).In group OD,the patient's satisfaction level was significantly higher than in group O (P ＜ 0.05).Nausea,vomiting and dizziness occurred in both groups without significantly difference between the two groups.Conclusion Oxycodone combined with dexmedetomidine can provide satisfactory analgesia for gastric cancer patients receiving laparoscopic surgery.

Standard therapy has not been established for thyroid cancer when a thyroidectomy is contraindicated due to systemic disease. Herein, we reported a patient who had hypertrophic cardiomyopathy and papillary thyroid carcinoma treated by radiofrequency ablation because of inability to tolerate a thyroidectomy. Radiofrequency ablation can be used to treat thyroid cancer when surgery is not feasible, although the long-term outcome needs further observation.
Cardiomyopathy, Hypertrophic* ; Catheter Ablation* ; Humans ; Thyroid Gland* ; Thyroid Neoplasms* ; Thyroidectomy

BACKGROUNDWith the increasing popularity of cosmetic facial filler injections in recent years, more and more associated complications have been reported. However, the causative surgical procedures and preventative measures have not been studied well up to now. The aim of this stady was to investigate the clinical characteristics and visual prognosis of fundus artery occlusion resulting from cosmetic facial filler injections.

METHODSThirteen consecutive patients with fundus artery occlusion caused by facial filler injections were included. Main outcome measures were filler materials, injection sites, best-corrected visual acuity (BCVA), fundus fluorescein angiography, and associated ocular and systemic manifestations.

RESULTSEleven patients had ophthalmic artery occlusion (OAO) and one patient each had central retinal artery occlusion (CRAO) and anterior ischemic optic neuropathy (AION). Injected materials included autologous fat (seven cases), hyaluronic acid (five cases), and bone collagen (one case). Injection sites were the frontal area (five cases), periocular area (two cases), temple area (two cases), and nose area and nasal area (4 cases). Injected autologous fat was associated with worse final BCVA than hyaluronic acid. The BCVA of seven patients with autologous fat injection in frontal area and temple area was no light perception. Most of the patients with OAO had ocular pain, headache, ptosis, ophthalmoplegia, and no improvement in final BCVA.

CONCLUSIONSCosmetic facial injections can cause fundus artery occlusion. Autologous fat injection tends to be associated with painful blindness, ptosis, ophthalmoplegia, and poor visual outcomes. The prognosis is much worse with autologous fat injection than hyaluronic acid injection.

Adult ; Cosmetic Techniques ; adverse effects ; Female ; Fundus Oculi ; Humans ; Injections, Intradermal ; adverse effects ; Male ; Middle Aged ; Retinal Artery Occlusion ; diagnosis ; etiology ; Visual Acuity ; physiology ; Young Adult