Objective To build the screening model for population with Impaired Glucose Regulation (IGR)in community, and to evaluate its validity by using the model in general population. Methods Based on the epidemiological survey of DM in the community population in 2006, the analysis of the risk factors and the risk factors scoring method were used to build the screening model for IGR in community which was then used to screen for IGR in 2 120 general population in community to evaluate its validity. Result When the screening model was built with the accumulative score of risk factors ≥5, the sensitivity and specificity for identifying undiagnosed IGR were 68.2% and 61.7%, Youden's index was 0.299 and the positive predictive value was 14.2%. When 2 120 general population used the screening model, only 974 (45.9% of them)had to take OGTT test, and 124 (68.9%) IGR were identified. Conclusion The screening model with the accumulative score of risk factors ≥5 is an easy, effective and economic tool for IGR screening, and it can be tried to use in the screening for IGR in community, further research is needed.

ObjectiveTo explore the effects of fluoxetine on special learning and memory and serum S100B level in depressed model rats.MethodsAdult male SD rats were divided into six groups randomly according random digits table:control group ( A ),depressed model group ( B ),group of depressed model treated with single dose of fluoxetine for one day ( C ),group of depressed model treated with fluoxetine for one week (D),group of depressed model treated with fluoxetine for two weeks (E) and group of depressed model treated with fluoxetine for four weeks (F),ten rats in each group.Except control group,others were subjected to forced-swimming for four weeks,15 min a day.Fluoxetine (10 mg/kg) was given intragastric administration to group C-F before swimming everyday.Morris water maze ( MWM ) was used to measure the spatial learning and memory of rats.ELISA was used to determine the level of serum S100B.ResultsIn the hiding platform test of MWM,there was significant longer of escape latency (EL) in B group than that in A group(P ＜ 0.05 ).And the EL in all groups treated with fluoxetine became shorter with the prolonging of treatment.In the probe test,there were significant longer time in target quadrant in D,E,F than in other quadrant (F =5.162,P ＜ 0.01 ).The levels of serum S100B were lower in E,F groups ( E group ( 0.91 ± 0.23 ) ng/ml,F group ( 0.85 ± 0.21 ) ng/ml) than that in B group (( 1.26 ±0.61 )ng/ml,P＜0.05).ConclusionChronic administration of fluoxetine could improve the impairment of spatial learning and memory and reverse the increase of S100B level in serum of depressed model rats.

Objective To establish the type Ⅱ collagen specific T cell line of Wistar rat and observe its effect on transferring arthritis.Methods The Wistar rats were immunized with emulsified chicken type Ⅱ collagen (CCⅡ) in complete Freund′s adjuvant by intradermal injection to induce the rat model of collagen induced arthritis (CIA).The lymphocytes were obtained from mesenteric lymph nodes of CIA rats,and the type Ⅱ collagen reactive T cell line was selected and propagated by CCⅡ stimulating in vitro .The proliferation response and phenotype were analyzed by 3 H TdR incorporation and fluorescence activated cell sorter (FACS).The onset of arthritis and pathological characteristic in ankle joints of recipient rats were observed with naked eye and histochemical examination.Anti CCⅡ antibody in serum was assayed by enzyme linked immunosorbent assay (ELISA).Results A T cell line was successfully established.The results of FACS labeled with fluorescent antibodies showed that 98 2% of the line cells were T cells,of which 89 7% were CD4 + T cells.The results of adoptive transfer showed that the incidence of arthritis was 50% when the injected cell number was 5?10 7,meanwhile the level of anti CCⅡ antibody in serum was elevated more than that of the control.Conclusion A cell line has been successfully established.The result of arthritis transferring by T cell line shows that the T cell plays a great role in the pathogenesis of CIA and provides a research datum for rheumatoid arthritis therapy with T cell vaccine.

Objective To compare the pharmacodynamics of common Bletilla striata powder(80 and 200 meshes) with ultra-fine powder of B.striata on experimental gastric ulcer in rats and to clarify how much the dose of B.striata was decreased after superfine comminution.Methods The models of gastric ulcer were set up by anhydrous alcohol and acetic acid to measure the gastric ulcer area and count out the inhibitory rate;The tissue slice of gastric ulcer cicatrix was got far HE staining.Based on the viewpoint of pathology the morbid state was observed.Results B.striata powder could decrease the gastric ulcer area significantly,and the groups of ultra-fine powder were advantaged to the others.Conclusion After superfine comminution,the potency of B.striata can be improved with its dose reducing.It is good for mo-dernization of making dosage forms.

Objective: To explore an efficient method for the establishment of 3D finite element model based on CBCT images. Methods: Mandible of a male volunteer was scanned by CBCT, and the resulting DICOM data was used for 3D reconstruction in Mimics17 software. Then with the. stl format file, the result of 3D reconstruction was imported into Geomagic Warp 2015, in which 3D models consisting of triangular patches for dentition, periodontal ligament and alveolar bone were created. With free meshing algorithm, the 3D finite element model of mandible with full dentition consisting of 10-node tetrahedron elements was obtained under the constraint that the maximum inner angle was set to be 25°. Results: The 3D finite element model for human mandible with full dentition was established. The total number of nodes is 299286, the elements number for dentition, periodontal ligament and alveolar bone are105805, 122427 and 577529, respectively. Conclusion: The proposed method can be used for the establishment of 3D finite element model of mandible with full dentition based on CBCT images, and it has the merits of good stability, high precision and wide application compared with the traditional modeling method.

Objective To study the association of glucagon like peptide 1 receptor (GLP1R) gene polymorphism with type 2 diabetes in Han population in Shanghai. Methods In the study, 360 type 2 diabetic patients and 313 normal control subjects were enrolled. Diabetic patients were further subdivided into insulin treated non obese patients (BMI28, 192 subjects). A single nucleotide polymorphism (SNP) rs 2268657 was genotyped in all the subjects enrolled in the study using allele specific real time PCR and its association with type 2 diabetes was examined. Results The frequencies of AA,AG, GG genotype incontrol group were0.086,0.447, 0.466 respectively, 0.155, 0.375, 0.470 in non obese diabetic patient group respectively, and 0.109, 0.500, 0.391 in obese diabetic patient group respectively. There was significant difference of the frequency of genotype AA between control group and non obese diabetic patient group (OR=1.939, P

Objective To investigate the incidence of early postpartum depression , postpartum depression occurs im-pact analysis related factors. Methods Self-Rating Anxiety Scale(SAS), Self-Rating Depression Scale(SDS), the Ed-inburgh Postnatal Depression Scale (EPDS) and family cohesion and adaptability scale(FACES) on 350 cases of prim-ipara prospective study. Results 51 cases of postpartum depression, the rate was 14.57%, Age of light, high level of education, poor neonatal cases, non-exclusive breastfeeding, household income, poor mothers were more likely to postpartum depression(P<0.05). Postpartum depression, maternal prenatal SAS, SDS scores and scores of family cohe-sion and adaptability normal pregnancy, the differences were statistically significant (P<0.05). Education, prenatal anxiety, prenatal depression, family adaptability and neonatal score were the main influencing factors of postpartum depression(P<0.05). Conclusion Initial prone to postpartum depression, prenatal psychological state, family support, education and neonatal cases of postpartum depression significantly affected , should strengthen targeted interventions to reduce the incidence of postpartum depression.

Objective To investigate the effect of self-directed learning applications in clinical nursing teaching. Methods In our department of nursing practice 63 nursing students were divided into the control group(n=31)and ob-servation group (n=32), respectively used traditional teaching methods and self-directed learning, test results,inde-pendent learning skills,thinking skills,communication skills of two groups were compared. Results The theoretical knowledge,operational skills and total scores of observation group were significantly higher than that in control group(P<0.05).The self-management skills,information capabilities,ability to cooperate and scores of observation group were significantly higher than that in control group(P<0.05). The open-minded,analytical ability,seek the truth,systematic,confidence and total score of observation group were significantly higher than that in control group(P<0.05).The inter-personal communication skills, coaching and consulting capabilities to provide effective feedback and negative scores of observation group were significantly higher than that in control group (P<0.05).Conclusion Self-directed learning is applied in gynecological care clinical teaching nursing students could effectively mobilize the enthusiasm and initiative of learn to improve the self-learning ability,critical thinking and communication skills,to improve the quality of clinical teaching .

Objective To observe the post-laparoscopic analgesic effect of oxycodone combined with dexmedetomidine for gastric cancer patients.Methods A total of 40 gastric cancer patients who underwent laparoscopy during November 2016 and January 2017 and assessed with physical status Ⅰ or Ⅱ according to American Society of Anesthesiologists,were randomly divided into 2 groups:oxycodone group (group O,n =20) and oxycodone plus dexmedetomidine group (group OD,n =20).The anestheasic effects at the time points of hours 2,5,8,11 and 24 after the operation were assessed by using visual analog pain score,times for pressing the pump,Ramsay score and patient's satisfaction index of the two groups.At the same time,the adverse effects of two groups were recorded and observed.Results The analgesic effect after starting the pump at hours 2,5,8,11,24 and 25,the VAS scores and the pressure time in group OD were significantly lower than in group O (P ＜ 0.05),and the Ramsay scores were insignificantly different between the groups (P ＞ 0.05).In group OD,the patient's satisfaction level was significantly higher than in group O (P ＜ 0.05).Nausea,vomiting and dizziness occurred in both groups without significantly difference between the two groups.Conclusion Oxycodone combined with dexmedetomidine can provide satisfactory analgesia for gastric cancer patients receiving laparoscopic surgery.