Objective To study the serum lipid levels and prevalence of lipid abnormalities in Uygur,Hasake,Mongolians,Han adults(≥30years)of Boertala region of Xinjiang.Methods From Sep.to Nov.2004,3 300 subjects were gathered from baseline survey of hypertension epidemiology in Boertala region.All participants had a physical examination and serum lipid test.Results TC age corrected mean levels were 4.77,4.69,4.86,4.64,HDL-C(mmol/L)was 1.33,1.46,1.48,1.41,LDL-C(mmol/L)was 2.78,2.65,2.70,2.48 TG(mmol/L);median TG was 1.15,1.06,1.18,1.28,prevalence of lipid abnormalities was 57.4%,46.6%,50.3%,51.8%in Uygur,Hasake,Mongolian and Han adults.Conclusion The mean lipid level is not high,but prevalence of lipid abnormalities is high in the subjects

ssociation of snoring with metabolic syndrome in the four ethnic populations in Boertala region of Xinjiang.

Objective To explore the relation of the expressions of MDM2 and VEGF in osteosarcoma with the pathological parameters and prognosis of the tumor.Methods The expressions of MDM2 and VEGF were detected with immunohistochemical(SP) method in specimens from 56 cases of osteosarcoma.The correlation between the expressions of MDM2,VEGF and pathological grade,metastasis and prognosis was analyzed statistically.while 8 cases of fibrous dysplasia of bone were used as negative control group.Results The positive rates of MDM2 and VEGF in osteosarcoma were 64.3%(36/56) and 67.9%(38/56),respectively .MDM2 and VEGF didn't express in negative control group.The expression of MDM2 and VEGF were not significantly correlated to the pathological grades of the osteosarcoma,but which were significantly correlated with tumor metastasis and prognosis(P

Objective To study the effects and the mechanism of the ethanol extract of Blueberry(BE) on relaxation vascular endothelium or smooth muscle.Methods To use rat aorta as the specimen,to observe the effects of BE on induced relaxation of the phenylephrine-precontracted aorta.and approach the mechanism on vascular endothelium or smooth muscle.Results BE induced relaxation of the phenylephrine-precontracted(1.0×10~(-5)mol·L~(-1) aorta in a dose-dependent way(P<0.01),which was disappeared by removal of functional endothelium(P<0.01).Pretreatment of the aortic tissues with NG-nitro-L-arginine methyl ester(L-NAME),methylene blue,or 1H[1,2,4]-oxadiazole-[4,3_a]-quinoxalin-1-one (ODQ) inhibited the vascular relaxation induced by BE(P<0.01).BE-induced vascular relaxations were also markedly attenuated by addition of verapamil or diltiazem,while the relaxant effect of BE was not blocked by pretreatment with indomethacine,glibenclamide,tetraethylammonium(TEA),atropine,propranolol(P<0.01).Conclusion These results suggest that BE dilates vascular smooth muscle via endothelium-dependent nitricoxide-cGMP signaling pathway,possible involvement of L-type Ca~(2+) channel.

Objective To investigate the genotype of Trp64Arg polymorphism of β3-adrenergic receptor gene and analyze the role ofβ3-adrenerglc receptor gene in the pathogenesis of morbid obesity and metabolic syndrome(MS).Methods PCR-restriction fragment length polymorphism Was used to detect the genotypes of 172 patients with MS,92 patient.with morbid obesity and 92 controls,and some biochemlcal indexes were detected.The association of the polymorohism with morbid obesity and MS Was assessed in case-control study.Resuits No statistically significant differences were found in the frequencies of Trp64Arg of β3-AR among the three groups.Conclusion Trp64Arg polymorphism of β3-AR was not significandy associated with morbid obesity and MS in the Hazak in xinjiang.

Objective To investigate the clinical value of thinprep cytology test(TCT) combined with colposcopy in diagnosis of cervical lesion. Methods Retrospective analysis was conducted in 174 females with abnormal cervical smear. All the patients underwent colposcopy to screen the cervical lesions. According to the pathological results,the clinical value of TCT and colposcopy was analyzed and compared. Results In the 174 cases with abnormal cervical smear,the positive rate of atypical squamous cells(ASC) was 27. 0% (47/174). There were 92 low grade squamous intraepithelial lesion (LSIL) (52. 9%) ,33 high grade squamous intraepithelial lesion (HSIL) (19. 0%) and 2 squamous cell cancinoma (SCC) (1. 1%) . According to the diagnosis by biopsy and pathology,the positive rate of benign cervical cell(BCC) was 16. 1% (28/174) .cervical intraepithelial neoplnsia (CIN) I 42. 0% (73/174), CIN II 18.4% (32/174), CIN ffl 21. 8% (38/174) and SCC 1. 7% (3/174) . The coincidence rate of TCT and pathology was 83. 9% (146/174). Conclusions TCT and colposcopy are a practical approach for detecting cervical lesion. TCT combined with colposcopy is the optimal screening approach for cervical lesion, which can enhance the sensitivity and specificity of the diagnosis of cervical lesions.

Objective To investigate the relationship between the color doppler sonography and contrast-enhanced echocardiography(CEE)in assessment of intrapulmonary shunting in patients with hepatopulmonary syndrome and HIF-1α,iNOS and portal hemodynamic.Methoods To examine the portal vein diameter and blood flow velocity by color Dop-pler,and intrapulmonary shunting by contrast-enhanced echocardiography(CEE).The expressions of HIF-1α and iNOS in serum were determined by ELISA in the normal,hepatocirrhosis amalgamating HPS and unamalgamating HPS separately.Comparison of HIF-1α and iNOS in plasma was carried out and relationship between the portal hemodynamic and the intra-pulmonary shunting was examined in three groups.Results The levels of HIF-1α and iNOS in hepatocirrhosis amalgamating HPS were higher than those in unamalgamating HPS,the diameter of portal vein dilated and the velocity of portal vein dropped,there was correlation(P＜0.05).There were positive correlation with intrapulmonary shunting.Conclusion The color doppler sonography and contrast-enhanced echocardiography (CEE)which reflected HIF-1α,iNOS and the hemodynamic played an role in developing of HPS,and was available to early examine change of intra-pulmonary vessel.

Objective To determine the relationship between neck circumference (NC) and cardiovascular risk factors among Uygur,Kazak,Mongolian and Han populations in Boertala Mongol Autonomous Prefecture of Xinjiang.Methods A total of 4299 adults (Uygur 1237,Kazak 1047,Mongolian 812,and Han 1203) aged 20 to 79 years old were selected from a cross-sectional study of metabolic syndrome in Boertala Mongol Autonomous Prefecture of Xinjiang.Pearson's correlation was used to assess the relationship between NC and body mass index (BMI),waist circumference (WC),waist-to-hip ration (WHR),systolic blood pressur (SBP),diastolic blood pressure (DBP),fasting blood glucose (FBG),total cholesterol (TC),low-density lipoprotein cholesterol (LDL-C),high-density lipoprotein cholesterol (HDL-C) and triglyceride (TG).Linear regression and Logistic regression were used to evaluate the effects of NC on SBP,DBP,FBG,TC,LDL-C,HDL-C,TG,hypertension,high FBG and lipid disorders among multiethnic population.Results Mean NC was (36.2 ± 3.8) cm in Uygur,(36.3 ± 4.1) cm in Kazak,(35.9 ± 3.6) cm in Mongolian and (35.7 ± 4.0) cm in Han,respectively.After adjusting age and gender,in Pearson's correlation coefficients,a significant association was found between NC and BMI (for Uygur,Kazak,Mongolian and Han,r values were 0.552,0.499,0.709 and 0.459,respectively; all P ＜ 0.05) or WC (for Uygur,Kazak,Mongolian and Han,r values were 0.525,0.479,0.695 and 0.376,respectively; all P ＜ 0.05).When BMI,WC and other factors were adjusted,linear regression indicated that FBG in Uygur (t =3.746,P ＜ 0.05) and LogTG in Mongolian (t =2.876 P ＜ 0.05) were increased with NC.In Logistic regression,increased NC was a risk factor of higher FBG in Uygur (odds ratio (OR) =1.139,95％confidence interval (CI):1.027-1.264) and lipid disorder in Mongolian (OR =1.156,95％ CI:1.009-1.325).Conclusions Our data suggest that NC may be associated with multiple risk factors of cardiovascular disease in Uygur,Kazak,Mongolian and Han adults,and the relationship could be different among multiethnic populations.It should be necessary to explore the role of upper-body fat in the development of metabolic disorders.

The clinical significance of a myeloperoxidase (MPO) gene polymorphism and inducible nitric oxide synthase (iNOS) expression in cirrhotic patients with hepatopulmonary syndrome (HPS) was explored. Enrolled subjects were divided into three groups according to their disease/health conditions: the HPS group (cirrhotic patients with HPS; n=63), the non-HPS group (cirrhotic patients without HPS; n=182), and the control group (healthy subjects without liver disease; n=35). The distribution of the MPO -463 G/A genotype and its relationship with iNOS expression in a typical cell block from ascitic fluid were detected by immunohistochemistry and polymerase chain reaction-restricted fragment length polymorphism analysis (PCR-RFLP). In the HPS group, the partial pressure of oxygen in blood and ascitic fluid was significantly decreased (8.95+/-1.58 kPa and 6.81+/-0.95 kPa, respectively; both P<0.01), while the partial pressure of carbon dioxide significantly increased (4.62+/-0.20 kPa and 5.92+/-0.45 kPa, respectively; P<0.01). MPO and iNOS levels were significantly increased in the HPS group as compared with the non-HPS group. These increases were even more remarkable in ascitic fluid (41.36+/-11.62 and 13.23+/-4.81 mug/L; 10.27+/- 3.20 and 4.95+/-1.12 mug/L) than in blood (16.66+/-5.24 and 4.87+/-1.73 mug/L; 5.79+/-2.31 and 2.35+/-0.84 mug/L). The distribution of the MPO genotypes GG, GA, and AA were 76.2%, 22.2% and 1.6% in the HPS group, and 57.7%, 37.9% and 4.4% in the non-HPS group (P<0.05). The expression of iNOS was significantly higher in patients with the G alleles (G/G and G/A) (61.54%, 48/78) than in patients with A alleles (G/A and A/A) (38.46%, 30/78) (P<0.01). It was suggested that the expression levels of iNOS and MPO were correlated with HPS-induced hypoxemia. The MPO-463 G/A mutation might be a protective factor that prevents the development of HPS. The MPO might be involved in the regulation of iNOS expression. In humans, MPO pathways, the iNOS/NO system, and their interaction might have an impact on the occurrence and development of HPS.

Objective:To observe the effect of Allicin in cardial fibroblasts (CFs) proliferation and Collagen secretion,and to explore its role on TLR4/NF-κB signal pathway.Methods:CFs of neonatal Wistar rats were isolated and cultured ,then was stimulated with AngⅡ.CFs proliferation was measured by thiazolyl blue ( MTT) assay.The expression of collagenⅠ,collagenⅢ was measured by ELISA.mRNA expression of TLR4 and NF-κB were detected by reverse transcription-polymerase chain reaction ,protein expression of TLR4 and NF-κB were detected with Western blot.Results: Allicin could reduced MTT value of cardial fibroblasts ( P<0.01 ) , and inhibited expression of collagenⅠ,collagenⅢ(P<0.01),which in a dose-dependent manner.Allicin could reduced mRNA expression of TLR4 and NF-κB and protein expression of TLR 4 and NF-κB in CF induced by Ang Ⅱ ( all P<0.01 ) .Conclusion: Allicin can inhibit Myocardial fibrosis ,which mechanism is possible by inhibiting TLR 4/NF-κB signal pathway.