MicroRNAs (miRNAs) are a class of endogenous;non-coding small RNA molecules;which can degradate target mRNA;or negatively regulate the expression of the corresponding target genes in the post transcriptional level through inhibiting target gene translation;inducing degradation and so on.MiRNAs exert important parts in the process of metabolism;cell growth;and development.MicroRNA 222 (miR-222) is an important member of microRNAs;which plays an important role in cell proliferation;differentiation;apoptosis and the development of a variety of biological tissues.Recent studies found that miR-222 could mediate a variety of physiological and pathological processes;and significantly influence the development of cardiovascular disease.MiR-222 has an important role in inflammatory response and apoptosis.Here;we reviewed the relationship between miR-222 and cardiovascular disease.

Objective To investigate the curative effect of Aidi injection solution combined withcarboplatin on controlling malignant pleural effusion(MPE).Method A total of 86 cancer patients with appearedPME were selected as our subjected who hospitalized in the First People's Hospital of Shangqiu from Feb.2012to Mar.2013.They were randomly divided into the observation group(43 cases) and the control group(43cases).Patients in the oontrol group were treated with carboplatin at dose of 100 mg/per times which was putinto the thoracic cavity,and patients in the observation group was treated with Aidi injection solution at dose of100 mg/per times beside carbeplatin.The treatment period was 4 weeks.The effect information,quality of lifeand adverse reactions were recorded.Results The total effective rate and survival quality improvement rate inobservation group were 86.05％ (37/43) and 51.16％ (22/43),higher than those in control group (67.44％(19/43),30.23％ (13/43)),and the differences were significant(x2 =4.170,3.903 ; P ＜ 0.05).There wasno significant difference in terms of the related adverse reactions between two groups(P ＞0.05).ConclusionThe therapy of Aidi injection solution plus carboplatin on controlling MPE is proved to be effective and it canimprove patients' quality of life with better safety.Therefore it is worthy of recommendation.

Objective To investigate the protective effects of aerobic exercise and miR-222 expression in myocardium of diabetic mice. Methods C57BL/ 6 mice were divided into 4 groups: normal non-exercise group (SC), normal exercise group(EC), non-exercise diabetic group(SD), and exercise diabetic group(ED). After the diabetic model was established successfully, EC and ED underwent a swimming training for 5 weeks. By the end of the experiment, light microscope was used to observe the pathological changes of heart, RT-PCR for myocardial miR-222 expression, and Western blot for phosphatase and tensin homolog deleted on chromosome ten ( PTEN ), phosphatidylinositol 3-kinase (PI3K), and protein kinase B (Akt) protein expressions in myocardial tissue. Results (1) Under the light microscope, the diabetic mice had a significant change in myocardial structure, with great disorder in the cell arrangement. After exercise intervention, the lesion was alleviated. (2) MiR-222 expression was increased in the myocardium of normal mice and DM mice after exercise (all P<0. 05); (3) Compared with SC group, PTEN expression was increased and PI3K/ Akt expressions were inhibited in myocardium of diabetic mice(all P <0. 05). After exercise intervention, the expression of PTEN reduced( P < 0. 05) and PI3K/ Akt pathway was reactivated in myocardium of diabetic mice (all P<0. 05). Conclusion Exercise intervention may protect the myocardium under high glucose via inducing miR-222 and activating PI3K/ Akt signaling pathway.

Objective To study the anti-inflammatory and analgesic effects of Jingtongning Capsule. Methods Models of acute inflammatory reactions of croton oil-induced mouse ear edema and carrageenan-induced rat raw swelling and chronic inflammatory reaction of cotton ball-induced mouse granuloma in abdominal cavity were performed to study the anti-inflammatory effects of Jingtongning Capsule. Model of acetic acid-induced writhing in mice was used to study the analgesic effect of Jingtongning Capsule. Results Jingtongning Capsule significantly suppressed croton oil-induced mouse ear edema and carrageenan-induce rat paw swelling. Cotton ball-induced mouse granuloma in abdominal cavity was also markedly reduced by Jingtongning Capsule. The writhing times of mice induced by acetic acid were decreased by Jingtongning Capsule. Conclusions Jingtongning Capsule has obvious anti-inflammatory effect on acute and chronic inflammatory reactions. The pain reactions induced by chemical stimulus was significantly relieved by Jingtongning Capsule.

Objective: To observe the therapeutical effects of Lipo PGE_ 1 in the treatment of diabetic peripheral neuropathy. Methods: 42 cases of type 2 diabetes patients with diabetic peripheral neuropathy were recruited and were divided into two groups at random:therapy group treated with Lipo PGE_ l and control group treated with Vitamine B_ 1 and Vitamine B_ l2 . Results: After a treatment of 20 days,symptoms such as pain,numbness and hypoesthesia in cases of therapy group had been improved at different degrees.Examination of Neuroelectrophysiology indicated an increase of sensory nerves conduction velocity and motor nerve conduction velocity.The effects of therapy group outweighed that of control group. Conclusion: Lipo PGE_ 1 can significantly relief the symptoms of diabetic peripheral neuropathy and increase nerve conduction velocity.The mechanism of action may involve improvement of blood-supply of nerve by vasodilatation and inhibition of platelet aggregation that caused by PGE_ l .

Objective To establish a method for determining paraquat in the serum by ultraviolet spectrophotometry and present a quantitative index for paraquat poisoning salvage. Methods The sample was deproteinizated by 20% trichloracetic acid(TAC). 50 ?l microcell was used, the detection wavelength was 257 nm. Results The linearity was within 0.05～50 ?g/ml, r=0.999 9. The average recovery rates were 90.0%-102.4% and RSD=3.9%(n=4), the lowest detection limit was 0.01 ?g/ml. Conclusion The method is simple, rapid, precise and suitable for the determination of paraquat in the serum.

ObjectiveTo provide evidence for the education on sychological health for the undergraduates by analyzing the relationship among interpersonal relationship, coping style, self consistency and congruence of local college students. Methods uestionnaires about interpersonal relationship, coping style, and self consis tency and congruence have been carried out on a stratified random sample of 1471 students from four local colle ges. And all the data of the questionnaire scales will be dealt with by the software SPSS15.0. Results①Total scores on interpersonal relationship and self consistency and congruence of male college students( (9.88 ±5.51 );(93.29 ± 13.48 ) respectively) were much higher than the scores of the female college students ( ( 8.84 ± 5.11 );(90.09 ± 12.60) respectively). Total scores of the above two items for the seniors( ( 10.26 ± 5.89 ); ( 96.79 ±12.93) respectively) were obviously higher than the total scores of students of other grades. For the engineering students, whose total scores on the two items( ( 10.26 ±5.86); (93.57 ± 12.83 ) respectively) were remarkably higher than the scores of students of other majors. Regarding the various factors of coping style, there are signifi cant differences in gender, grade and major( P ＜ 0. 01 ). ②Concerning the college students, there were conspicu ous relationship among their interpersonal relationship, coping style, self consistency and congruence ( P ＜ 0.01 ). ③ Self consistency and congruence could be effectively predicted by factors, i.e. total score of interpersonai rela tionship, remorse, problem-solving, rationalizing, fantasy, recoil, help-seeking and grade. ConclusionCollege students' ability of self consistency and congruence is affected by their coping style and interpersonal relationship. To improve the individual' s ability of self consistency and congruence and the condition of their psychological health, the most effective way is to cultivate the capability on interpersonal communication and apply the positively flexible coping style.

Objective To study and compare the present situation and the difference in subjective well-being between students in junior high schools for the blind and normal junior high school students, and provide basis for the construction of education mode for the well-being of the blind students. Methods By random sampling method, subjective well-being questionnaire was used to investigate the 155 blind students in five blind schools and 436 normal junior high school students in three junior high schools in Shandong Province. Results ( 1 ) The blind students had shown significant differences in many aspects of subjective well-being such as gender, family e-conomic status, sight and parents'education degree (P<0. 05) . (2) Blind school students'social confidence experience (4. 59 ±1.13) was significantly higher than that of the normal students (4.31 ±1.13), and had a significant difference (P<0.05). Blind students got significantly lower scores ((4. 38 ± 1.26) , (4.00 ± 1. 29) ,(3. 58 ± 1. 37) ,(3.89 ± 1. 35) ,(4.41 ± 1.04) , (4. 20 ± 1. 33) ) than normal school students( (4.68 ± 1. 19) , (4. 36 ±1.14),(3.88±1.27),(4.41 ±1.25),(4.61 ±0.99), (4. 52 ± 1. 18)) in target value experience, physical health experience, mental health experience, interpersonal adaptability experience self-acceptance experience, and emotional balance experience. Conclusion There exists a significant difference between the blind students and the normal junior high school students in the different aspects of subjective well-being.

Objective To compare medical students' learning curve between Macintosh laryngoscope and optical laryngoscope in endotracheal intubation and to decide which one is more suitable for novice personnel.Methods Totally 26 interns after being trained by experienced anesthesiologists were enrolled in this study.They performed intubation on 6 patients by using both Macintosh laryngoscope and optical laryngoscope ( each laryngoscope for 3 patients) under the supervision of experienced anesthesiologists.The sequence of laryngoscope was determined by computer-generated random number list.Results Totally 149 patients who were ready to receive surgery under general anesthesia were enrolled in this randomized crossover controlled study.Duration of intubation was significantly shorter in Airtraq group (78 -± 33 ) s than in Macintosh group (114 ± 32) s,P ＜ 0.001.Intubation success rate was significantly higher in Airtraq group than in Macintosh group ( 87.8％ vs.66.7％,P ＜ 0.05 ).Conclusions With the advantages of rapid learning curve,higher intubation success rate and shorter intubation duration,Airtraq laryngoscope is easier to master for novice personnel.

Objective To explore the association of T cell immunoglobulin- and mucin-domain-containing molecule-3 (TIM-3) gene polymorphism and IFN-γ levels with the incidence of primary nephrotic syndrome (PNS) in children. Methods A case-control study was conducted and 21 Han patients with PNS were selected and included in case group. Meanwhile, 20 each from Mongolian and Han were selected and assigned into control group and at least three generations of their family members were from the same nationality. PCR-restriction fragment polymorphism analysis was used to detect and analyze single nucleotide polymorphisms of exon -574A/C in TIM-3 gene in PNS children and controls. Also the genotype and allele frequencies between the two groups were compared. Enzyme linked immunosorbent assay (ELISA) was used to detect the level of serum IFN-γ and its changes was analyzed. Results There was no significant difference in the distribution of genotypes (AA, AC, CC) of exon -574A/C in TIM-3 gene between the Han and Mongolian subgroups in control group (P=0.741). Neither did the allele frequency between the two groups (P=0.655). Compared with control group (Han and Mongolian), the frequencies of AA, AC and CC genotypes were 9.52%, 28.57% and 61.90% respectively in -574A/C loci of the Han nationality children with PNS. There was significant difference in genotypes distribution between the two groups (P=0.017). The frequency of C allele in PNS children of Han nationality was 76.2% which was higher than that in normal control group (50%), and the difference was statistically significant (P=0.005). Compared with A allele carriers, the risk of PNS in C allele carriers increased by 3.20 times (95%CI: 1.39～7.37). There were no significant differences in serum IFN-γ among the Han nationality with PNS, Han and Mongolian normal control groups (P>0.05). Conclusion The single nucleotide polymorphism of the exon -574A/C of TIM-3 gene may be related to the pathogenesis of PNS in children. In addition, IFN-γ is not associated with the incidence of primary nephrotic syndrome in children.