Objective To investigate the protective effects of aerobic exercise and miR-222 expression in myocardium of diabetic mice. Methods C57BL/ 6 mice were divided into 4 groups: normal non-exercise group (SC), normal exercise group(EC), non-exercise diabetic group(SD), and exercise diabetic group(ED). After the diabetic model was established successfully, EC and ED underwent a swimming training for 5 weeks. By the end of the experiment, light microscope was used to observe the pathological changes of heart, RT-PCR for myocardial miR-222 expression, and Western blot for phosphatase and tensin homolog deleted on chromosome ten ( PTEN ), phosphatidylinositol 3-kinase (PI3K), and protein kinase B (Akt) protein expressions in myocardial tissue. Results (1) Under the light microscope, the diabetic mice had a significant change in myocardial structure, with great disorder in the cell arrangement. After exercise intervention, the lesion was alleviated. (2) MiR-222 expression was increased in the myocardium of normal mice and DM mice after exercise (all P<0. 05); (3) Compared with SC group, PTEN expression was increased and PI3K/ Akt expressions were inhibited in myocardium of diabetic mice(all P <0. 05). After exercise intervention, the expression of PTEN reduced( P < 0. 05) and PI3K/ Akt pathway was reactivated in myocardium of diabetic mice (all P<0. 05). Conclusion Exercise intervention may protect the myocardium under high glucose via inducing miR-222 and activating PI3K/ Akt signaling pathway.

Objective To investigate the curative effect of Aidi injection solution combined withcarboplatin on controlling malignant pleural effusion(MPE).Method A total of 86 cancer patients with appearedPME were selected as our subjected who hospitalized in the First People's Hospital of Shangqiu from Feb.2012to Mar.2013.They were randomly divided into the observation group(43 cases) and the control group(43cases).Patients in the oontrol group were treated with carboplatin at dose of 100 mg/per times which was putinto the thoracic cavity,and patients in the observation group was treated with Aidi injection solution at dose of100 mg/per times beside carbeplatin.The treatment period was 4 weeks.The effect information,quality of lifeand adverse reactions were recorded.Results The total effective rate and survival quality improvement rate inobservation group were 86.05％ (37/43) and 51.16％ (22/43),higher than those in control group (67.44％(19/43),30.23％ (13/43)),and the differences were significant(x2 =4.170,3.903 ; P ＜ 0.05).There wasno significant difference in terms of the related adverse reactions between two groups(P ＞0.05).ConclusionThe therapy of Aidi injection solution plus carboplatin on controlling MPE is proved to be effective and it canimprove patients' quality of life with better safety.Therefore it is worthy of recommendation.

MicroRNAs (miRNAs) are a class of endogenous;non-coding small RNA molecules;which can degradate target mRNA;or negatively regulate the expression of the corresponding target genes in the post transcriptional level through inhibiting target gene translation;inducing degradation and so on.MiRNAs exert important parts in the process of metabolism;cell growth;and development.MicroRNA 222 (miR-222) is an important member of microRNAs;which plays an important role in cell proliferation;differentiation;apoptosis and the development of a variety of biological tissues.Recent studies found that miR-222 could mediate a variety of physiological and pathological processes;and significantly influence the development of cardiovascular disease.MiR-222 has an important role in inflammatory response and apoptosis.Here;we reviewed the relationship between miR-222 and cardiovascular disease.

Objective To compare medical students' learning curve between Macintosh laryngoscope and optical laryngoscope in endotracheal intubation and to decide which one is more suitable for novice personnel.Methods Totally 26 interns after being trained by experienced anesthesiologists were enrolled in this study.They performed intubation on 6 patients by using both Macintosh laryngoscope and optical laryngoscope ( each laryngoscope for 3 patients) under the supervision of experienced anesthesiologists.The sequence of laryngoscope was determined by computer-generated random number list.Results Totally 149 patients who were ready to receive surgery under general anesthesia were enrolled in this randomized crossover controlled study.Duration of intubation was significantly shorter in Airtraq group (78 -± 33 ) s than in Macintosh group (114 ± 32) s,P ＜ 0.001.Intubation success rate was significantly higher in Airtraq group than in Macintosh group ( 87.8％ vs.66.7％,P ＜ 0.05 ).Conclusions With the advantages of rapid learning curve,higher intubation success rate and shorter intubation duration,Airtraq laryngoscope is easier to master for novice personnel.

Objective To study the therapeutic effect of pulse-activating injection on acute poisoning by pa-raquat(PQ). Methods 50 Sprague-Dawley (SD) rats were randomly divided into five experimental groups: blank group, negative control group, positive control group, low-dose pulse-activating injection group (LDG), and high-dose pulse-activating injection group(HDG) (n = 10 for each group). Blank group were injected with normal suline,30 ml/kg,and other groups were established as acute paraquat poisoning models. Macroscopic and histopathological ex-aminations were performed and biological indexes were measured for the lung specimens. The indexes included lung wet weight/dry weight,the rats of neutrophils and protein content in the pulmonary alveolar lavage fluid. In the mean time, the level of malondialdehyde (MDA), superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) both in plasma and bronchoalveolar lavage flui(BALF) were detected. Results Compared with that in negative control group,lung congestion and lung edema of LDG group were mitigated; and the MDA level decreased from (5.04± 0.50) nmol/ml,(1. 19±0.18) nmol/ml to (4.04±0.21) nmol/ml,(0.79±0.04) nmol/ml both in plasma and BALF;the SOD activities were increased from (123.30±20. 39) U/ml, (26.43±2.22) U/ml to (277.09± 11.66) U/ml,(37.10± 2.49) U/ml as well; the GSH-Px activities were increased from (1796.63 ±81. 12) U/ml, (598.24 ± 62.50) U/ml to (2151.54 ± 148.32) U/ml, ( 1788.44 ± 175.11 ) U/ml as well ( P < 0. 01 ). Conclusions Administration of pulse-activating injection could improve the lipid peroxidation damage caused by a-cute poisoning of PQ.

Objective:To screen the issues of management for radiation therapy equipment in an academic hospital with Delphi method.Methods: 6 specialists with different background were consulted with the questionnaire, and those questions were screened from questionnaire as being scored with the importance and analyzed by SPSS software.Results: 6 specialists included 2 radiation managers, 3 radiation professionals and 1 health administrative personnel. The authority coefficient was 0.88±0.06, and the activity coefficient was 100%. The Kendall concordance coefficient wereW=0.019(P=0.635) andW=0.237(P=0.001) in the 1st and 2nd round respectively. The coordination of the expert’s opinions was good and the results of the questionnaire were credible. There were 18 questions got from the questionnaire based on the averages and standard deviations.Conclusion: It was effectiveness that Delphi method can screen the issues of management for radiation equipment, on which we can get the direction of performance improvement based.

AIM: To study the protective effect of aerobic exercise on cardiac dysfunction in mice and its mechanism, and to provide theoretical and practical basis for the exercise therapy of diabetic cardiac dysfunction .METH-ODS:The mice were divided into normal control non-exercise (NNC) group, normal control exercise (ENC) group, dia-betic non-exercise (NDM) group and diabetic exercise (EDM) group.At the end of the experiment , the cardiac function was evaluated by echocardiography .The pathological changes of the myocardial tissues and the development of fibrosis were observed.The mRNA expression of ANP, and the protein levels of PI3K (p110α) and Akt were determined.RESULTS:The decrease in cardiac function of diabetic mice was observed , and the cardiac function recovered after exercise interven-tion (P<0.05).Under light microscope with HE and Masson staining , the myocardial structure in NDM group was in ex-treme disorder , cell arrangement was not neat , and the degree of fibrosis increased , but the myocardial damage was im-proved in ENC group .Compared with NNC group , the mRNA expression of ANP in the myocardium of diabetic mice was up-regulated (P<0.05).The protein levels of PI3K (p110α) and Akt were decreased (P<0.05), and the cascade was inactivated.Compared with NDM group , the mRNA expression of ANP was down-regulated and the protein levels of PI 3K (p110α) and Akt were up-regulated in EDM group (P<0.05).CONCLUSION:Diabetes results in myocardial damage in mice, and reduces cardiac function .Exercise intervention alleviates the heart dysfunction induced by high glucose via activating PI3K( p110α)/Akt signaling pathway to protect the structure and function of the myocardium .

Objective:To evaluate the acute toxicity and protective effects of polysaccharide from Schisandrae sphenantherae Fruc-tus in the mice with acute liver injury induced by CCl4 . Methods:The classical acute toxicity tests were used to determine the maxi-mum drug loading (MLD) and observe the acute toxicity. The acute liver injury model was induced by 0. 5% CCl4. Sixty mice were randomly divided into the blank control group, CCl4 model group, bifendatatum model group and polysaccharide group respectively at high (1388 mg·kg-1), medium (694 mg·kg-1) and low (347 mg·kg-1) dose groups with 10 ones in each. After 6-day drug treatment, the levels of aspartate aminotransferase ( AST) and alanine aminotransferase ( ALT) were determined by kits. Histopatho-logical examination was also carried out. Results: General behavior, body weight and organ examination in the acute toxicity tests showed no obvious changes. Compared with those in the model group, the levels of AST in the three polysaccharide groups significantly decreased (P<0. 05 or P<0. 01), the levels of ALT in polysaccharide group at high and medium doses significantly decreased(P<0. 01), and the liver tissue damage was improved to a certain extent as well. Conclusion: Polysaccharide from Schisandrae sphenan-therae Fructus has protective effects on acute liver injury induced by CCl4 in mice with low acute toxicity.

Objective To establish a method for determining paraquat in the serum by ultraviolet spectrophotometry and present a quantitative index for paraquat poisoning salvage. Methods The sample was deproteinizated by 20% trichloracetic acid(TAC). 50 ?l microcell was used, the detection wavelength was 257 nm. Results The linearity was within 0.05～50 ?g/ml, r=0.999 9. The average recovery rates were 90.0%-102.4% and RSD=3.9%(n=4), the lowest detection limit was 0.01 ?g/ml. Conclusion The method is simple, rapid, precise and suitable for the determination of paraquat in the serum.

Objective To investigate the efficacy of recombinant human erythropoietin(rHuEPO) in treating the anemia in obstrics. Methods 40 antepartum anemia patients and 36 postpartum anemia patients were treated with rHuEPO 8000IU intravenously. Another 40 antepartum and 36 postpartum anemia patients were selected as control. Oral supplementation of iron and folic acid were used both in study and control group. Results The hemoglobin level was increased significantly in the study group than control group both in the prenatal and postpartum anemia patients. ( P0.05 )。 Conclusion rHuEPO therapy for obstetric anemia is effective and safe.