Objective To study the change of the peripheral blood cells of soldiers in high altitude hypoxia,to analyze its influ-ence on the blood cells.Methods The blood cell were compared among the soldiers in the plains,entered plateau after 6 months and stationed plateau for a long time,and to analyzed the effect of high altitude hypoxia environment on blood cells.Results WBC, RBC,hemoglobin,hematocrit and mean corpuscular hemoglobin concentration were obviously above plain group and long staff group (P <0.05),and mean corpuscular volume was obviously lower than those of plain group and long staff group (P <0.05).White cell and lymphocytes in plateau group were also obviously higher than those in plain group (P <0.05),and long staff group no signifi-cantly differences (P >0.05).platelet,mean platelet volume,platelet distribution width was clearly higher than that of plain group (P <0.05 ).Conclusion Blood cells of soldiers training in the high altitude hypoxia environment are significantly increased,and changes in blood cells are closely related to emplacement time and altitude.

Objective To investigate the therapeutic effect of inhaled budesonide suspension combined with psychological intervention on children with asthma. Methods 64 children with asthma were enrolled in our hospital from October 2015 to December 2016. They were divided into experimental group and control group, 32 cases in each group. The control group was treated with inhaled budesonide suspension.The experimental group was treated with inhalation with budesonide suspension combined with psychological intervention, the treatment efficiency and symptom improvement were compared and analyzed in the two groups. Results The effective rate of the experimental group was 96.87%, which was higher than that of the control group 84.37%, the difference was statistically significant (P<0.05). The symptoms of cough, wheezing, wheezing and dyspnea in the experimental group was obviously better than that in the control group, the difference was statistically significant (P<0.05). Conclusion The effect of inhalation with budesonide suspension combined with psychological intervention in the treatment of children with asthmais pbvious.It can effectively improve the clinical symptoms, and promote recovery of children.

OBJECTIVE:To evaluate the application effects of PDCA cycle management in the outpatient prescription intervention.METHODS:A total of outpatient prescriptions (pre-intervention group) during Jan.-Dec.2014 and 918 659 (intervention group) were selected during Jan.-Dec.2015 were selected from a hospital.Irrational 9utpatient prescriptions were analyzed before and after the application of PDCA cycle management,and the improvement of main indexes were compared such as unsuitable usage and dosage,incomplete clinical diagnosis writing,no physicians' signature on the prescription or inconsistent with sample.RE SULTS:Before intervention,there were 2 347 irrational prescriptions,including 1 401 nonstandard prescriptions,849 unsuitable prescriptions and 97 abnormal prescriptions.After intervention,there were 1 161 irrational prescriptions,including 695 nonstandard prescriptions,425 unsuitable prescription and 41 abnormal prescriptions,decreasing 50.53％,50.39％ 49.94％ and 57.73％,respectively.The proportion of unsuitable usage and dosage,incomplete clinical diagnosis writing,no physicians' signature on the prescription or inconsistent with sample,nonstandard prescription paper,unsuitable indications,no signature and date for prescription revision or no reason and new signature for overdose use,outpatient prescriptions more than 7 d common dose or emergency prescriptions more than 3 d common dose without special situation in total amount of outpatient prescriptions decreased from 0.72‰,0.57‰,0.45‰,0.27‰,0.20‰,0.19‰ and 0.15‰ before intervention to 0.32‰,0.25‰,0.19‰,0.11‰,0.09‰,0.08‰ and 0.07‰ after intervention.CONCLUSIONS:PDCA cycle management significantly improves the quality of outpatient prescriptions.There still are irrational outpatient prescriptions in this hospital,and it is to be intervened continuously.

Objective To investigate the relationship between the single nucleotide polymorphisms (SNP) of rs1008438 in HSP70 promoter and the susceptibility of high altitude pulmonary edema (HAPE) .Methods The PCR‐DNA sequencing method was used to analyze gene distribution of rs1008438 in 100 HAPE patients and 200 healthy people ,and the relationship between dif‐ferent genotypes and HAPE was evaluated .Meanwhile ,HSP70 protein in cytoplasm and nuclei of white blood cells were detected by ELISA in patientgroup and healthy group .TNF‐α,IL‐1β and IL‐6 levels were analyzed by protein chip technology and EVI‐DENCE180 automatic chip reader .Results The TT ,GT ,and GG genetype frequencies of rs1008438 in HAPE patients and healthy controls were 76 .0% ,20 .0% ,4 .0% ,and 93 .0% ,6 .5% ,0 .5% ,respectively .The TT genetype frequency in HAPE patients was significantly lower than that in healthy control(P<0 .05) .The HAPE incidence rate in GT/GG genetype was 4 .195 times higher than that in TT genetype ,and the allele G can also significantly increase the prevalence of HAPE compared to T allele (OR=4 .178) .ELISA showed that HSP70 were higher in HAPE group of all genotypes than those in healthy control of the same geno‐type .And the nuclei/cytoplasm ratio of HSP70 in TT genotype was higher than that in GT/GG genotype .Protein chip showed that the levels of TNF α,IL‐1β,and IL‐6 in HAPE group were significantly higher than those in healthy control .Conclusion The poly‐morphism of rs1008438 is related to susceptibility of HAPE .Mutate genetype may change the promoter activity and increase the ex‐pression of HSP70 ,which induced HAPE .

Objective:The purpose of this study was to observe Huwang Mingmu San’s(HWMMS)effect on the experimental vitreous hemorrhage.Methods:We divided 48 rabbits randomly into the A group as the normal control group,the B group as the modal group,the C group as the traditional chinese medicine group(HWMMS)and the D group as the western medicine group(entodan).Six rabbits of the four groups were killed randomly in the 8th week and their vitreous send to TNF-?,IL-6 examination at the same time.We study the activity level of MDA,SOD,NA-K-ATP enzyme on retinal.Results: It was suggested that the TNF-?’s content in HWMMS group had significantly increased than that in the normal group and the model group but no difference was found between the HWMMS group and the entodan group.Meanwhile the results showed that the HWMMS group’s content of IL-6 and MDA was lower and SOD activity,NA-K-ATP enzyme content was higher than those in the model group and the entodan group.Conclusion:We concluded that the HEMMS had a remarkable therapeutic effect on retinal damage and in promoting absorption vitreous hemorrhage.

The distribution of hepatitis B virus genotype in Hubei province and its clinical significance were investigated. HBV genotypes of 276 patients were detected by PCR-microplate sandwich hybridization-ELISA technique. The level of HBV DNA was detected by using PCR-fluorescence quantification test. Among 276 patients, there were 78 cases of HBV asymptomatic carriers, 110 cases of chronic hepatitis B (CHB), 62 cases of severe hepatitis (SH) or liver cirrhosis (LC) and 26 cases of hepatocellular carcinoma (HCC). The genotypes of HBV included C, B, mixtures (B+C, B+D, C+D) and D, accounting for 55.8%, 25.4%, 16.7% and 2.1% respectively. The average level of HBV DNA in genotypes C, B, mixtures and D was 1.20x10(6), 7.81x10(4), 3.26x10(5) and 5.01x10(4) copies/mL respectively. The ratio of SH, LC and HCC in genotype B, C and mixtures was 20%, 30% and 48% respectively. Statistical analysis revealed the percentage of genotype mixtures infection was significantly higher than that of genotype B infection. There was no significant difference in the percentage between genotype B and genotype C or between genotype C and mixtures. The distribution of genotype B, C and mixtures in SH, LC and HCC was significantly different. The frequency of HCC was zero in patients with co-infection. Genotype D was only related with SH and LC. The increased ALT could be converted to categorical grades of severity. From mild, moderate to severity, the prevalence of genotype C showed an opposite trend, although no statistically significant difference was observed. The HBeAg positive rate was higher in patients with genotype C infection than in those with genotype B, especially in the patients whose ages were from 31 to 40 years old. Compared with genotype B, genotype C showed a higher HBeAg positive rate in patients with SH and LC. The percentage of SH, LC and HCC was higher in patients with genotype C and mixtures infection. On the contrary, the percentage of genotype B was lower. The HBeAg positive rate in patients with genotype C infection was higher than those with genotype B infection. Genotype C and mixtures may be associated with development of severe liver disease.

OBJECTIVE:To provide reference for reasonable iron supplement during pregnancy. METHODS:5023 iron sup-plement prescriptions for 800 delivery women receiving antenatal care and childbearing in our hospital in 2014 were analyzed retro-spectively. According to international authoritative guideline for gestational iron supplement,the rationality of iron supplement for pregnant women in our hospital was investigated in terms of timing,duration and dosage. RESULTS:The iron supplement timing and duration of 800 pregnant women all met the criteria;among 5023 iron supplement prescriptions,62 prescriptions of preventive iron supplement and 3875 prescriptions of iron supplement for iron deficiency were up to the standard. CONCLUSIONS:The dos-age of gestational iron supplement are unreasonable in our hospital at a certain extent. It is necessary to strengthen clinical medica-tion management to guarantee safe and rational iron supplement in pregnant women.

Objective To describe the mastery of knowledge about antihypertensive drugs of clinical nurses in one tertiary comprehensive hospital of Beijing.Methods Purposive sampling was used to recruit 195 clinical nurses who were working in one tertiary comprehensive hospital in Beijing.The demographic questionnaire,knowledge of antihypertensive drugs questionnaire were filled.Results The score of knowledge of antihypertensive drugs Questionnaire was (45.63 ± 3.79) points;different education,wishes to take part in hypertension related knowledge training had significant differences (t =2.007,2.049,P ＜ 0.05).Conclusion Knowledge of antihypertensive drugs of clinical nurses in Beijing was at medium level.Those who indicated that it was not matter whether to attend hypertension related knowledge training and had low level of education had more problems in knowledge of antihypertensive drugs.

OBJECTIVE To investigate the pre-operation and/or pre-transfusion testing on patients to avoid risk of(occupational) infection exposure among medical staff and its significance in prevention of nosocomial infection.METHODS Serum hepatitis B(virus surface) antigen(HBsAg),antibody against hepatitis C virus(anti-HCV),(antibody) against human(immunodeficiency) virus(anti-HIV) and toluidine red unheatedserum test(TRUST) were performed on samples from 10 745 cases of pre-operation and/or pre-transfusion from 2003 to 2004.RESULTS The number and(percentage) of positive reaction for HBsAg,anti-HCV,anti-HIV and TRUST were 1163,(10.81%);21,0.20%;1,0.01%(confirmed by HIV Reference Laboratory,Center for Disease Prevention and(Control) of Zhejiang Province) and 74,(0.69%),respectively.TRUST positivity was further confirmed with TPHA.(Overlapping) infections were mostly observed between HBV and HCV,or,to a less scale,between HBV and syphilis.For HBV infection the(department) of digestive medicine was prevalent(?~2=4.77,P

Objective To investigate recurrent spontaneous abortion patients received bends the clinical application effect of nursing intervention during progesterone treatment. Methods Two patients with recurrent spontaneous abortion group were treated with progesterone treatment, the control group in the conventional nursing service only provides flexor progesterone treatment on the basis of comprehensive nursing service study group progesterone treatment on the basis of routine the combination of nursing and nursing intervention in the 2 groups of patients with recurrent spontaneous abortion. The success rate of pregnancy were recorded, the rate of abortion, the data will be given the statistical analysis (using SPSS software) after the conclusion. Results The study group of patients with recurrent spontaneous abortion pregnancy success rate (95.45%) and control group (90.91%) there is no significant difference between the study group of patients with recurrent spontaneous abortion abortion rate (6.82%) was significantly lower than the control group (34.09%), there was significant difference between the groups, P<0.05. Conclusion Recurrence During the treatment of flexor progesterone abortion were given routine nursing and nursing intervention combined with the comprehensive clinical nursing service can obtain ideal success rate of pregnancy, to reduce the rate of abortion is also of positive significance, is conducive to the protection of patients' quality of life, physical and mental health.