Objective To investigate the role of FHIT,cyclinD1,CDK4 in NSCLC.Methods Immunochemical(S-P) was used to determined 81 cases of NSCLC.Results The postive expression rates of FHIT,cyclinD1,CDK4 were significantly different between in NSCLC and in nomal lung tisses(P

Professional personality is characterized by stability and plasticity,and it should make the entry point of the cultivation of medical students' professional personality on the training of doctor-patient communication skills.Meanwhile,doctor-patient communication skill is an important factor of the medical professional personality.This paper discussed the cultivation of doctor-patient communication skills among medical students from the perspective of analysis of doctor-patient communication obstacles.It should improve the doctor-patient communication skills of medical students and shape healthy medical professional personality,taking the education of cognition,emotion,will,and behavior as pathways.

OBJECTIVE:To detect and analyze the drug resistance of the AmpC and the extended-spectrum ?-lactamases(ESBLs)in Escherichia coli(E coli)from urinary infections.METHODS:ESBLs was detected by Phenotypic Confirmatory Test and AmpC was detected by improved three dimensional test.RESULTS:The isolating rates of ESBLs(45 strains),AmpC(8 strains)and AmpC+ESBLs in E.coli(5 strains)were 34.6%,6.2% and 3.8%,respectively.The drug resistance rates of ESBLs and AmpC-producing E.coli was higher than those of the strains without producing ESBLs or AmpC.CONCLUSION:Production of AmpC and ESBLs in ?-lactam antibiotics was the main resistance mechanism against E.coli.Carbopenems should be regarded as the first choice in clinical empirical medication for enzyme-producing strains.

0.05), but there is statistic difference between the two groups in estimating the involvement of cervical canal and the earlier surgical stage than clinical stage(P

Objective To explore the association of T cell immunoglobulin- and mucin-domain-containing molecule-3 (TIM-3) gene polymorphism and IFN-γ levels with the incidence of primary nephrotic syndrome (PNS) in children. Methods A case-control study was conducted and 21 Han patients with PNS were selected and included in case group. Meanwhile, 20 each from Mongolian and Han were selected and assigned into control group and at least three generations of their family members were from the same nationality. PCR-restriction fragment polymorphism analysis was used to detect and analyze single nucleotide polymorphisms of exon -574A/C in TIM-3 gene in PNS children and controls. Also the genotype and allele frequencies between the two groups were compared. Enzyme linked immunosorbent assay (ELISA) was used to detect the level of serum IFN-γ and its changes was analyzed. Results There was no significant difference in the distribution of genotypes (AA, AC, CC) of exon -574A/C in TIM-3 gene between the Han and Mongolian subgroups in control group (P=0.741). Neither did the allele frequency between the two groups (P=0.655). Compared with control group (Han and Mongolian), the frequencies of AA, AC and CC genotypes were 9.52%, 28.57% and 61.90% respectively in -574A/C loci of the Han nationality children with PNS. There was significant difference in genotypes distribution between the two groups (P=0.017). The frequency of C allele in PNS children of Han nationality was 76.2% which was higher than that in normal control group (50%), and the difference was statistically significant (P=0.005). Compared with A allele carriers, the risk of PNS in C allele carriers increased by 3.20 times (95%CI: 1.39～7.37). There were no significant differences in serum IFN-γ among the Han nationality with PNS, Han and Mongolian normal control groups (P>0.05). Conclusion The single nucleotide polymorphism of the exon -574A/C of TIM-3 gene may be related to the pathogenesis of PNS in children. In addition, IFN-γ is not associated with the incidence of primary nephrotic syndrome in children.

Objective To investigate the role of FHIT in NSCLC and the relativity between FHIT and cigarette.Methods 81 cases of NSCLC and 7 cases of the nomal lung tisses were examined for FHIT by immunochemical(S-P).Results The postive expression rates of FHIT were significantly lower than those in nomal lung tisses(P

Objective To investigate the expressions and significance of PAX-5, OCT-2 and BOB.1 in Hodgkin lymphoma (HL). Methods Immunohistochemical method was used to detect the expressions of CD30, CD15, PAX-5, OCT-2, BOB.1, CD20 and CD3 in 44 samples of formalin-fixed, paraffin-embedded tissues of HL. The histological classification was carried out according to the WHO classification for tumors of hematopoietic and lymphoid tissues (2001). Results In all 44 cases, 3 (6.8) were nodular lymphocyte predominant Hodgkin lymphoma (NLPHL) and 41 (93.2) were classical Hodgkin lymphoma (CHL). In Hodgkin and Reed Sternberg cells, CD30 were expressed in 36 samples (81.8), CD15 in 27 (61.4), CD20 in 14 (31.8), PAX-5 in 43 (97.7), OCT-2 in 14 (31.8), and BOB.1 in 7 (15.9). All samples of NLPHL expressed OCT-2 and BOB.1, while in the samples of CHL, 30 samples (73.2) expressed both OCT-2 and BOB.1, 7 (17.1) expressed only OCT-2, 1 (2.4) expressed only BOB.1 and all were negative for CD30. Conclusion Hodgkin lymphoma is a rare neoplasm with uncertain cause and difficult to diagnose. The detection of PAX-5, OCT-2 , BOB.1 in combination with CD30, CD15 will conduce to the diagnosis and differential diagnosis of Hodgkin lymphoma.

Objective To evaluate the applicability of small molecular markers of nephrotoxicity that in prediction of drug toxicity.Method Extracts of five kinds of traditional Chinese medicines (Tripterygium wilfordii,Strychni semen,Aristolochiafangchi,Rhei Radix et Rhizoma,and Xanthium sibiricum) that had known as nephrotoxicity were ig given to rats to establish renal injury models,and the blood samples were collected after administration for 1 and 7 d.Then blood samples were analyzed by UPLC/Q-TOF-MS for five kinds of small molecule biomarkers-thymidine,lyso-phosphatidylcholine (LPC 16:1),LPC (18:4),LPC (20:5),and LPC (22:5).The support vector machine (SVM) prediction model was established to determine the toxicity.The levels of Cr and BUN in serum were determined by automatic biochemical analyzer.The rats in each group were sacrificed after blood collection,and the kidneys were taken for HE staining.Result No toxicity was observed in the control group,and the biochemical test results showed no renal injury after mentioned five kinds Chinese herbs were given for 1 d,while SVM model of nephrotoxicity had been found abnormal.After administration for 7 d,the results of SVM model show renal toxicity,which were consistent with biochemical and pathological examination.Conclusion Metabonomics combined with the earlier established SVM model enabled prediction of drug nephrotoxicity more sensitively,quickly and \ccurately,and it is of great significance for the discovery of drug toxicity as well as the prevention and treatment of drug-induced renal injuries in clinic.

Objective To investigate the water infectivity in key water regions,and analyze the transmission risk of schisto-somiasis in suspicious and high-risk environments in Hubei Province in 2016. Methods Schistosome-endemic areas of the Yangtze River,the Hanbei River and the Fu River were chosen as the surveillance and forecast sites. The water infectivity was detected by using the sentinel mice during the flood season. The infection status of residents in the villages around the surveil-lance sites and the activities of human beings and domestic animals were surveyed. The emergency response system was initiated when the water infectivity areas were detected. Results From May to June,among the ten surveillance sites of the first batch, two positive spots with infected mice were found,accounting for 20％. All the recovered mice(totally 200)were dissected:five mice were infected,with a total infection rate of 2.5％. Totally five schistosome adult worms were collected,with mean worm bur-den of 1 worm per infected mouse. The site with cercariae-infected water body started the emergency response and no epidemic occurred. From August to September,among the eight surveillance sites of the second batch,the recovered mice(totally 160) were dissected,and no infected sentinel mice were found. Conclusion The sentinel mice method plays an important role in an-alyzing the schistosomiasis transmission risk in Hubei Province.

OBJECTIVE: To explore the effect and mechanism of kidney-warming and astringent therapy in treating nephrotic syndrome patients with deficiency of spleen and kidney yang and overflow of water, and to observe the change of plasma endothelin and interleukin-2 receptor after treatment. METHODS: Forty-four patients were randomly divided into conventional steroid treated group (control group, 20 cases) and conventional steroid plus kidney-warming and astringent therapy treated group (treatment group, 24 cases). The levels of plasma endothelin (ET) and soluble interleukin-2 receptor (sIL-2) were observed. RESULTS: Before treatment, plasma ET and sIL-2 in the patients were significantly higher than those in healthy people (P<0.01). After treatment, the ET and sIL-2 levels were obviously improved in both treated groups (P<0.05) and the improvement in the treatment group was more obvious. The difference between the two treated groups after treatment was significant (P<0.05). CONCLUSION: Conventional treatment plus kidney-warming and astringent therapy can effectively improve the levels of plasma ET and sIL-2 in treating nephrotic syndrome patients with deficiency of spleen and kidney yang and overflow of water, and hence alleviate the damage of renal tissue.