Shenghua decoction made by FU Qing-zhu is a famous formula aimed at syndrome of coagulation cold due to blood deficiency,static blood obstruction.The mechanism of disease is definite when this formula is applied.But the extensive use of Shenghua decoction,especially some potent preparation of it following childbirth without according to TCM principle based on differentiation of syndromes is a manifestation of highlighting formula and underestimating theory,which is useless and harmful.It should be emphasized.So the Shenghua decoction should be used under the direction of basic theory of TCM,through precise syndrome differentiation,according to the principle of "looking to and not being besieged by postpartum ".We should not follow blindly the method of treatment of "warming and resolving stasis" on the postpartum disease.It is important that Shenghua decoction should be used on the basis of syndrome differentiation.

Objective:To detect the biological activity of the expression products of the human ?-NGF cDNA in CHO cell line and purified the seperated protein.Methods:Human ?-NGF was transfected with lipofectamine reagent into CHO cell line.The biological activity was analyzed by objection with microscope and the method of MTT.The pure protein was proved by SDS-PAGE analysis.Results:The protein in the culture supernatant of the positive CHO cells transfected with ?-NGF gene could stimulate the growth of PC12 cell line and go into BBB.Conclusion:The target gene expressed successfully in the transfected CHO cell line and had good biological activity. [

Objective:To study the healing effect of Chitin dressing on second-degree scalds in mice and rabbits respectively.Methods:The model of the second-degree scald was established in mice and rabbits respectively by vapour and hot water;the healing time was observed and the tissue specimens were obtained from the scalds for pathologic analysis.Results:Compared with the contrast group,the healing time in chitin dressing treated group was shorter(P

To study whether the late-acting co-stimulatory molecules ICOS can suppress the apoptosis and sustain the survival and proliferation of T cells through the survivin pathway, ICOS signals deficient T-cells were infected with adenovirus carried survivin gene, other T-cells were given ICOS co-stimulatory signals, then infected with adenovirus carried dominant-negative mutant survivin gene. Apoptosis and proliferation were determined by TUNEL and CCK-8 respectively. The results show that engagement of ICOS signal increased the expression level of survivin significantly. Survivin can sustain co-stimulatory deficient T cells survival and suppress the apoptosis. Mutant survivin inhibits ICOS signal positive T cells survival and increase its apoptosis. Late-acting co-stimulatory molecules ICOS can suppress the apoptosis and sustain the survival of T cells through the survivin pathway.

AIM:To explore the effects of galangal (Alpinia officinarum Hance) extract on learning ability and memory consolidation and clearance of the free radical in mice. METHODS:Ninety mice receiving sodium nitrite to form the dysmnesic model ones,which were used to observe the walking behaviours in Morris water maze under the administration of galangal extact. SOD activity and the MDA content were measured with colorimetry,The morphological change in hippocampus slices were assessed using H-E staining and microscopic examination. RESULTS:To compare with the model group,the escape latency was markedly shortened(P

To establish a rat model of frostbite and to evaluate the effects of different administration methods of Huangqi Guizhi Wuwu Decoction (HGWD), a compound traditional Chinese herbal medicine for warming meridians to disperse cold, on rats with frostbite.

H type hypertension is essential hypertension complicated elevated plasma homocysteine level ,which ac-counts for about 75% in Chinese adults with hypertension and is closely related to cerebral stroke and other cardio-vascular diseases .Lowering homocysteine level in patients with hypertension possesses important significance for preventing stroke .

Objective: To investigate the smoking cessation intention and attempt to quit smoking among smokers at ages of 15 years and older in a district of Beijing Municipality, so as to provide insights into formulation of tobacco control interventions. Methods: Permanent residents at ages of 15 years and older were sampled using a multi-stage stratified cluster sampling method from a district in Beijing Municipality, and all smokers were recruited. Participants' demographic features, tobacco use, intention to quit smoking, attempts to quit smoking and awareness of tobacco-related hazards were collected using the Beijing Adult Tobacco Survey. The intention and attempts to quit smoking were analyzed among smokers, and factors affecting the attempt to quit smoking were identified using a multivariable logistic regression model. Results: A total of 687 smokers were surveyed, including 669 men (97.38%), 497 from rural areas (72.34%), 351 daily smokers (51.09%), 336 occasional smokers (48.91%), 329 with intention to quit smoking (47.89%), and 178 with attempts to quit smoking during the past one year (25.91%). Univariable analysis showed that area, age, educational level, smoking status, tobacco health literacy and tobacco control information acquired from media were factors affecting intention and attempts to quit smoking among smokers (P<0.05). Multivariable logistic regression analysis showed that smokers with intention to quit smoking (OR=5.444, 95%CI: 3.585-8.268) and occasional smoking (OR=2.142, 95%CI: 1.312-3.497) were more likely to attempt to quit smoking. Conclusions Approximately half of smokers have intention to quit smoking in a district of Beijing Municipality; however, the percentage of attempts to quit smoking is low. Targeted interventions are required for smokers with different characteristics to improve the intention to quit smoking and promote smoking-quitting behaviors.

Objective To perform mutation analysis of survival motor neuron gene 1 (SMN1 in two spinal muscular atrophy (SMA) patients and their parents to evaluate the effects of the two SMN1 gene mutations on the transcript levels of the gene and preliminarily predict their effects on the structure and function of SMN protein.Methods Mutation analysis of SMN1 gene was carried out by multiplex ligationdependent probe amplification,reverse transcript-polymerase chain reaction (RT-PCR) and cloning sequencing.Transmission of the mutations was confirmed by the mutation analysis in patients' parents.The full-length SMN1 (SMN1-fl) transcript levels of the patients carrying these subtle mutations were detected using quantitative RT-PCR.Results The two patients were diagnosed as SMA Ⅱ and SMA Ⅲ.They carried p.Val19GlyfsX21 and p.Ala2Gly SMN1 mutations in SMN1 gene,respectively.Both of the two mutations were originated from their fathers.Compared with the healthy individuals (23.5 ± 4.9),the two patients had a significant reduction in the level of SMN1-fl transcripts (t =3.322,P =0.011 (p.Ala2Gly) ;t =6.964,P =0.000 (p.Val19GlyfsX21)).However,compared with the healthy carriers (14.1 ±4.5),the patient with p.Ala2Gly mutation had no significant reduction in the level of SMN1-fl transcripts (13.9 ±3.6,t =0.058,P =0.955) ; however,the patient with p.Val19GlyfsX21 mutation had a significant reduction (4.9± 2.4,t =3.725,P =0.004).Conclusions Two SMN1 gene mutations are identified in our study.The mutation p.Val19GlyfsX21 is a novel mutation and p.Ala2Gly is firstly reported in Chinese SMA patients.p.Val19GlyfsX21 may possibly lead to decreased SMN1-fl mRNA by nonsense-mediated messenger RNA decay,however,p.Ala2Gly has no obvious effects on the amount of the SMN1-fl transcripts,indicating that its deleterious effect may be occurring at SMN protein level or the function of SMN protein.

Objective Our previous study demonstrated that toll-like receptor 2(TLR2) can distinguish extraneous antigen and prevent the immunological response. This study was designed to detect the expression of toll-like receptor 2 (TLR2) mRNA in cornea and investigate the effect of steroid on TLR2 expression in rats cornea following allograft penetrating keratoplasty. Methods The penetrating keratoplasty models were established in SPF rats with the 108 SD rats as receiptors and 36 SPF Wistar rats as donors, and other 6 SPF SD rats worked as normal controls. The receiptor rats were divided randomly into autograft group, allograft group and steriod group. The clarity and neovascularization of corneas of experiment rats were examined under the slit-lamp microscope and the rejection index was calculated based on Holland criteria. Corneal histopathological examination was carried out by hemotoxylin and eosin staining under the light microscope, and real time-PCR was employed for the detect of TLR2 mRNA in the corneas at the fifth, seventh and ninth day after operation. The experimental animals were obtained from the Animal Experimental Center of Southern Medical University and the procedure followed the Statement of Association for Research in Vision and Ophthalmology. Results The rejection occurred in 7 days after operation in allograft group, and only mild edema, opacity and neovascularization of corneas were found at different degrees in 9 days after operation in autograft group and steriod group. Severe corneal edema, a lots of inflammatory cells infiltration and new vessels in stroma were seen in allograft group, and mild inflammatory response was found in autograft group and steriod group. Normal comeal structure was exhibited in normal control group under the light microscope. The fold differences of TLR2 mRNA expression in cornea after amplification was significantly different among three groups and different time points (F_(group) = 39. 46, P = 0. 00; F_(time) =35. 38, P = 0. 00 ; F_(interaction) = 45. 66, P =0. 00), and the evident enhance of TLR2 mRNA expression was revealed in allograft group compared with autograft group (P ＜ 0. 05) and declined in steriod group (P ＜ 0. 05). Conclusion Steriod may restrain the acute allograft rejection by down-regulating the expression of TLR2 in corneas and its signals transaction. This result suggests that steriod offer a protection from rejection of cornea after penetrating keratoplasty.