In recent years, with the introduction of novel vectors capable of highly efficient transduction ,ribozymes and dexyribozymes have been become an ideal approach to anticancer therapy due to their high efficiency and lack of severe adverse effects. As many high efficiency targets have been found for cancer therapy, targeted therapy using ribozymes and deoxyribozymes may have multiple effects such as induction of tumor apoptosis and inhibition of tumor growth, metastasis, and angiogenesis.

Fragile X syndrome is a common cause of inherit mental retardation,GABAA receptors are the major inhibitory neurotransmitter receptros in the mammalian central nervous system, implicated in anxiety,depression, epilepsy, sleeping problem, learning and memory, and so on. Several researches indicated that the GABAergic system, in particular the GABAA receptors might be related to the phenotype of fragile X syndrome. An altered expression of certain subunits of the GABAA receptors may cause the neurological and behavioural changes of the fragile X syndrome. Therefore,the GABAA receptors can be a novel therapeutic target for treatment of fragile X syndrome.

Ghrelin is involved in the process of feeding behavior and regulation of related energy metabolism,it also modulates the secretion of gonadotropins or transmitters concerning sex hormone releasing.It is considered as the factor connecting the HPG axis and energy metabolism.Body's nutrition has a bearing upon the process of reproductive system's onset and maturation.Adequate body weight is necessary for normal puberty onset.Obese children tend to have an earlier menarche.Ghrelin may pass on the information about nutrition to the hypothamalus in puberty onset.

ObjectiveTo observe the effect of amlodipine besylate combined with benazepril in treatment of old patients with primary hypertension and left ventricular hypertrophy,and analyze the safety.MethodsOne hundred and eight old patients with primary hypertension and left ventricular hypertrophy were divided into observation group(54 cases) and control group(54 cases) by random digits table.The patients in observation group were treated with amlodipine besylate combined with benazepril and the patients in control group were only treated with amlodipine besylate.The levels of blood pressure,interventricular septal thickness(IVST),left ventricular posterior wall (LVPW),left ventricular end-diastolic dimension (LVEDD) and left ventficular mass index(LVMI) before and after treatment for 6 months were measured and compared between two groups.The adverse effects after treatment were compared too.ResultsAfter treatment,the levels of 24hSBP,24hDBP,IVST,LVPW,LVEDD and LVMI were significantly decreased in both groups (P ＜ 0.05).Moreover,the levels of 24hSBP,24hDBP,IVST,LVPW,LVEDD and LVMI after treatment in observation group were significantly lower than those in control group(P ＜ 0.05).There was 1 case ( 1.9％ ) of dry cough,2 cases (3.7％) of mild ankle edema in observation group,while only 2 cases (3.7％) of mild ankle edema in control group.There was no significant difference in adverse effects between two groups (P ＞ 0.05).ConclusionsCompared with amlodipine besylate single treatment,amlodipine besylate combined with benazepril in treatment of old patients with primary hypertension and left ventricular hypertrophy can more effectively control blood pressure and reverse left ventricular hypertrophy.The adverse effects are not increased,which can be applied in clinic.

Insulin resistance is the fundamental defect of type 2 diabetes and often leads to hyperinsulinemia, which is associated with atherosclerosis and cardiovascular events. A multidisciplinary approach involving multifaceted therapeutic strategies is necessary to reduce cardiovascular events and to improve prognosis in these patients. The purpose of this review is to outline proven and promising therapeutic strategies for improving the long-term cardiovascular prognosis of patients with type 2 diabetes.

The management of extremely low birth weigh infants is challenging,including how to communicate with family members,cooperation between pediatric and obstetrics,overall NICU management level,targeted and personalized management mode,and etc.In this article,clinical management experience for extremely low birth weigh infants was summarized,such as management at birth,stabilization of body temperature after birth,prevention and treatment of intracranial hemorrhage,infection,and blood transfusion management,as well post-discharge management.

Th17 is a CD4+ T cell subset that is a new member of Th1 and tH2.Recent studies have shown that it products IL-17A,IL-17F,IL-21 and IL-22,which play an imoortant role in rheumatoid arthritis.systemic lupus erythematosus,systemic sclerosis,inflammatory howel disease.autoimmune myocarditis and other autoimmune diseases.Deepen the understanding of Th17 and Th17 production in the advancement of autoimmune diseases will provide a new basis for future diagnosis.Th17 will become a new target for the treatment of human autoimmune diseases.

MicroRNAs (miRNAs) are a class of small single-stranded and non-coding RNAs that negatively regulate gene expression at the post-transcriptional level by degradation of target mRNA or inhibition of protein translation.Recent studies have shown that miRNAs play important roles in human diseases.It is of great importance to explore the role of miRNAs in the pathogenesis,early diagnosis and new therapeutic strategies of a variety of malignant tumors,including B-cell lymphoma.This review described the biosynthesis of miRNAs,the mechanisms of miRNAs target regulation and the involvement of miRNAs in the initiation and progression of human cancers,and summarized the role of miRNAs in B-cell lymphoma.

Objective To analyze the curative effect and safety of the estrogen as an adjunctive treatment of senile vaginitis.Methods 100 patients with senile vaginitis in our hospital from June 2011 to September 2012 were divided into observation group (n =50) and control group (n =50).Observation group was given estrogen plus metronidazole and control group was given metronidazole only.Results 45 cases were cured and 4 cases had effective treatment in observation group,and 21 cases were cured and 17 cases had effective treatment in control group.The efficacy rate was 98.0％ (49 cases)in observation group versus 76.0％(38 cases) in control group (x2=11.50,P＜0.01).After the treatment,the recurrence rate was 6.1％ (3 cases)and 26.3 ％ (10 cases)in observation and control groups respectively (x2 =10.66,P＜0.05).After treatment,vaginal health scores,symptom scores of vaginitis,and vaginal PH were significantly higher in the observation group than in the control group (all P＜0.05).In addition,the improvements of signs and symptoms were significantly better in the observation group than in the control group (all P＜0.05).However,there was no significant difference in adverse reaction rate between the two groups (P＞ 0.05).Conclusions Estrogen as an adjunctive treatment of senile vaginitis has better curative effect and safety.It is worthy of generalization in clinical medicine.

Biolog EcoPlateTM is a mainstream approach of studying community-level physiological profiles of microbes. To correct misunderstanding and insufficiency of experimental result explanation, we announced an amendment of information extraction of Biolog EcoPlateTM, which included two parts: (1) carbon substrates assortment sheets based on microbial physiological metabolism and ecological function, providing referrence and index for researchers; (2) illustration of how to use assorment sheet and ordination method to find out more information, guiding by environmental concentration principle.