Snake compound (SC)consisted of the alcoholic extract of Zaocys dhumnades,Agkistrodon halys(Pallas)compounded with Chinese herbs: Astragalus membranaceus Bge. var. mongholicus (Bge.) Hsiao, Angelica sinensis (Oliv) Diels, Rehmannia glutinosa Libosch, Paris pollyphylla Sm. var. yunancnsis, Epimedium brevicornum Maxim and Salvia miltiorrhiza Bge.. Acute toxicity test showed that the maximum tolerant oral dose for mice was 120mg/kg, 1091 times higher than the dose used in clinic for patients. Analgesic and anti-inflammatory studies showed that the times of writhe induced by abdominal administration of acetic acid were decreased significantly and the pain thre shold of electrical stimuli was increased markedly by SC in mice. The analgesic effects were dose-dependent. Inflammatory swelling in digiti pedis of rats induced by carrageenin and swelling of ear in mice were alleviated dramatically by SC.SC showed good effects in the treatment of winddampness syndrome and anthralgia syndrome in clinic.

Objective To observe the curative effect and safety of Xiaoer zhixie decoction in treatment children with infectious diarrhea.Methods 84 cases with infectious diarrhea were randomly divided into 3 groups (positive control group,Xiaoer zhixie decoction group and control group).The control group (n =27) was treated with conventional therapy such as nutritional therapy.The Xiaoer zhixie decoction group (n =28) was given Xiaoer zhixie decoction through clysis.Positive control group(n =29) was treated with oral.Smectite powder.The therapeutic effect of 3 groups was observed during the 3rd day,the 5th day and the 7th day after treatment.Results The total effective rate of control group was 40.7％,positive control group was 79.3％ and Xiaoer zhixie decoction group was 78.6％ after 3 days treatment.Compared with control group,positive control group and Xiaoer zhixie decoction group achieved obvious effect(P ＜0.01).The positive control group and Xiaoer zhixie decoction group did not have significant difference(P ＞0.05).Conclusion The clinical effect of Xiaoer zhixie decoction in treatment children with infectious diarrhea is significant.

Pain is one of usual symptons of mid-late cancer patients, radioactive particle interstitial im- plantation can not only control tumor advancement,but also alleviate pain in different degree by inactivating part nerves or relieving compression. It has showed significant effect in system tumor--gastric cancer, colon cancer and head and neck cancer without severe cemplication,effering a new method of curing cancerous pain.

Aim To observe the effect of ramipril on ischemia-reperfusion induced apoptosis of cardiomyocytes and expression of apoptosis-related genes.Method Male Wistar rats were subjected to 30 min of myocardial ischemia and 120 min of reperfusion.Rats were randomized to receive vehicle or ramipril(1 mg?kg~(-1)) orally 24 h before surgery.Myocardial infarct size was measured using the staining agent 2,3,5-triphenyl tetrazolium chloride(TTC).Cardiomyocyte apoptosis was assessed using terminal deoxyribonucleotidyl transferase-mediated dUTP nick-end labeling(TUNEL) assay and confirmed with detection of DNA ladder formation.The expression of antiapoptotic gene(Bcl-2) and proapoptotic gene(Bax) was determined by immunohistochemistry.Results At the end of reperfusion,infarct size was reduced by ramipril.TUNEL assay and DNA-laddering study demonstrated that cardiomyocyte apoptosis was attenuated in ramipril-treated hearts,correlating with increased expression of Bcl-2 and ratio of Bcl-2/Bax.Conclusion Ramipril exerts cardioprotection by decreasing the ischemia-reperfusion induced necrosis and apoptosis of cardiomyocyte and increasing the expression of Bcl-2 and ratio of Bcl-2/Bax.

Recently the cases that doctors or hospitals show indifferently or unconcerned towards patients in mortal danger occur repeatedly,which can not be contained simply by moral condemnation and restraint.Considering its social hazards and correlated ancient and modern legislations,it is a direct and effective way to criminalize this immoral behavior.However,doctors' or hospitals' indifference towards patients in mortal danger is not an isolated issue,but closely correlated with other social contradictions and conflicts.Therefore,a fine social assistance system of medical service is still in great need so as to comprehensively solve this problem.

Biochemistry is an essential course for medical students,which updates fast,develops quickly and has strong characteristic of experimentation.Opening experiment is a new open type experimental teaching mode and students are regarded as main body.Opening experiment is helpful to stimulating activeness and cultivating innovative consciousness of students as an effective supplement for traditional experimental teaching.This kind of teaching mode is in conformance with experimental teaching reform in university and is an effective way of cultivating medical science talented men.

Objective:The pathogenesis of placental vascular disease(PVD) was further investigated. Methode:PVD group (study group) and normal pregnancy (control group) were recruited. Two-channel mi-croarray technique was applied to screen the most differently expressive genes of human genome, and veri-fied the different expressions in mRNA and protein levels by quantitative PCR and Westem-blot, also local-ized the expression of different genes in placental tissue by immunohistochemistry. Reeults:HspA6 and Hs-pA1A had extremely higher expression in placentas with PVD than normal pregnancy. These genes both en-coded Hsp70. Hsp70 was mostly expressed in endothelial and smooth muscle cells of placental microves-ssls. The expression of Hsp70 mRNA and protein were up-regulated in placental tissue and microvascular endothelial cells from PVD group compared with normal pregnancy ( P <0.05) ;The expression of Hsp70 mR-NA and protein had negative correlation with infant birth weight ( P < 0.05). Conclueione:Hsp70 was associ-ated with endothelial activation in the pathogenesis of PVD by initiating an innate immune response and in-flammatory reaction.

Objective:To investigate the role of Leukotactin-1(LKN-1),CC receptor 1(CCR1)and CC receptor 3(CCR3)in the pathogenesis of hypertensive disorder complicating pregnancy(HDCP),the expressions of these factors in placentas were measured.Methods:The levels of LKN-1,CCR1 and CCR3 in placentas were measured from 32 patients with HDCP(study group)and 32 normal pregnant women(control group)by western blot(WB).The localization of these factorsin placentas were detected by immunohistochemistry(IHC).Results:LKN-1 protein expression was negative in placentas from the two groups by WB and IHC,while both CCR1 and CCR3 in the HDCP group were significantly lower than those in the control group(CCR1 0.66±0.06 vs 0.88±0.03.CCR3 0.17±0.01 vs 0.27±0.01,P<0.01).CCR1 was localized in vascular endothelial cells and stroma cells in both groups,but rare in the trophoblast layer.CCR3 was localized in vascular endothelial cells and trophoblast cells in both groups.Conclusions:There is no LKN-1expression in placentas from the two groups.Down regulation of CCR1 and CCR3 may associate with the pathogenesis of HDCP.

Objective To study some biological properties of mouse bone marrow stromal cells(MSCs)and their ability to differentiate endothelial cells and surviving ability in ischemic tissue,and provide an experimental foundation for applying MSCs to ischemic repair.Methods After the tibias and femurs were dissected from 5-to 6-week-old mice from Jan.2005 to Nov.2005,the marrow was flushed out with ice-cold DMEM/F12 medium.The mononuclear cells of the marrow were obtained with density gradient centrifugation and the plated and cultured in DMEM/F12 medium.The cultured cells in vitro were induced with endothelial cell growth supplement.The ability of MSCs was also was examined in ischemic tissue.Results The adherent fibroblast-shaped cells approached confluence in single layer 12~16 d after plating.The cultured MSCs in vitro differentiated into endothelium.Numerous scattered DAPI-labeled cells were found in the specimen seven days after implantation,and hematoxylin and eosin staining of the adjacent section showed concordance between dense hematoxylin staining and presence of DAPI epifluorescence,and there was no obvious inflammatory response.Conclusion The subcultured MSCs possess potential to differentiate into endothelial cells.MSCs show stable growth in vitro,easy survival in the subculture and rapid proliferation in present culture condition.MSCs may be used in therapy for myocardium ischemia.

0.05).Patients with mucitis of the study group and the control group were 0 and 7.The difference in statistics analysis was significant(P