Objective To explore the effect of periodic reporting system applied in clini-cal teaching.Method 62 nursing students of grade 2006 and 2007 practising in our hospital were selected and each group leaders reported their practical information to the teaching regula-tor every other month.Result If nursing students rehearsed the operational procedures and the items and communication skills that the teachers asked them to pay attention to,nursing stu-dents would be more self-confident in practice. Meanwhile,about 70% teachers didn’t introduce the working environment or clinical nursing knowledge to nursing students.Instead,they made them run errands.Conclusion Periodic reporting system was conducive to nursing students’suc-cessful completion of the internship program,and conducive to teaching regulators to understand nursing students’practical demands and the overall level of clinical teaching,which increased the overall level of clinical teaching and nursing students’satisfaction of teaching.

Ryanodine receptor is a Ca2+ release channel in cadiocyte.It is reported recently to the obvious change of its function and quantity in such pathological process as the myocardial ischemia and cardiac hypertrophy.These changes will affect the ability of cadiocytes processing intracellular calcium or lead to the calcium overload in the myocardial cells and then induces fatal ventricular arrhythmia,even sudden cardiac death.

Objective To observe the degradation pattern of tubulin in the cardiac muscle and lung tissue of rats after death.Methods 24 young male Sprague-Dawley rats were divided into 6 groups averagely.After being killed by bleeding,the rats were kept in an artificial climate incubator;the experimental temperature was kept at 20℃ and humidity at 50%.Proteins in cardiac muscle and lung tissue were extracted and tubulin was then examined by Western blot.Thereafter,the semi-quantitative analysis of the Western blot images was performed.Results Tubulin can be detected in the normal cardiac muscle and lung tissue of rats.After 1d postmortem,the tubulin content in these tissues began to decline,and it might still be detected at 7d postmortem,but the tubulin content in lung decreased faster than that in cardiac muscle.A linear relationship between tubulin content in cardiac muscle and lung of rats and the postmortem interval was determined.The linear regression equations and coefficients for integral optic density in relation to PMI were y=-1726.1x+14083 with correlative coefficient r=0.9684 for cardiac muscle and y=-1439.89x+12041 with correlative coefficient r=0.9808 for lung,respectively.Conclusion The level of tubulin in cardiac muscle and lung tissue of rats varied with PMI and the time dependent degradation of this protein was potentially useful for the PMI estimation.

Objective To clone amastin coding genes from different strains of Leishmania parasites. Methods Using amastin cDNA sequence as the reference, dbEST data base established by National Center Biotechnology Information (NCBI), USA, was searched by BLAST tool. A 309 bp DNA fragment of Leishmania major was found and used as the probe for the screening of a DNA library. The amastin gene of Leishmania major Abdou was cloned and sequenced. Specific primers were designed and amastin genes for Leishmania mexicana WR972, Leishmania brizeliensis and Leishmania amazonensis joseph were amplified by polymerase chain reaction. Results The amastin genes from 4 strains of Leishmania parasites were cloned and sequenced. It was found that all 4 amastin genes contained unique open reading frame of 552 bp and encoded amastin protein of 183 amino acid residues. Conclusion The amastin genes of 4 strains of Leishmania parasites were successfully cloned.

Objective To observe the degradation of tubulin in liver,spleen and kidney of rats killed by bleeding and to explore its usefulness in estimation of postmortem interval(PMI).Methods 24 young male Sprague-Dawley rats were killed by bleeding and kept in an artificial climate incubator for 0,1,2,3,5 and 7d respectively,then the content of tubulin in spleen,liver,and kidney were examined by western-blot assay and the data of detection were analyzed statistically by using SPSS 11.5 software.Results Within a certain postmortem interval(PMI

Objective:To investigate the stress distribution of mandibular implant-supported complete overdenture and supporting tissue with different location of implants.Methods:By three-dimentional finite element method, the stress distribution in the supporting tissue and overdenture supported by implants located in the interforaminal region or bilateral canines and first molars area was analyzed.Results:The overdenture supported by implants located in the interforaminal region showed poor oblique loading resistance, the peak value of stress in implant-bone interface was 2.4-9.2 times higher than that of vertial loading. Under vertical or oblique loading conditions, there was higher stress concentration in implants and base plates. The overdenture supported by implants located in bilateral canions and first molars area manifested better mechanical characteristic, the peak value of stress in supporting tissue was in the physiological range of bone.Conclusion:The location of implants in molar area can enhance the ability of overdenture to resist oblique loading, reduce the peak value of stress in implant-bone interface.

Objective To investigate the effect and clinical value of color Doppler ultrasound detection of blood flow parameters and vascular diameter in carotid artery and vertebral artery.Methods 150 healthcases were collected as the study objects.According to the age and gender differences, making a detailed classification, color Doppler ultrasound was used to detected the internal carotid artery, vertebral artery and the carotid artery.Results As age grows,the blood flow velocity decreases,and the pulsatility index( PI) and resistance index( RI) has no obvi-ous relation with age;There were positive correlation between the diameter of the carotid artery and vertebral artery and age( P<0.05) ,and the male population the diameter of the carotid artery was significantly wider than that of the female population(P<0.05),left vertebral artery diameter was significantly wider than that of the right side(P<0.05).Conclusion Color Doppler ultrasound detection can let us get detailed carotid and vertebral artery anatomy and blood supply of information,which is good to diagnosis cerebrovascular disease.

Objective To investigate the prevalence of low back pain among adults in Beijing.Methods The study design was a cross-sectional study,and the multi-stage sampling was used.A questionnaire survey was conducted in December 2010 to investigate prevalence of low back pain in adults who had lived in Beijing for over 6 months.Total prevalence and prevalence by region,gender and age were calculated.The chi-square test was used to compare results.Results A total of 3860 people were enrolled in this study.The one-year prevalence of low back pain was 26.09％ (1007/3860),and the point prevalence was 6.11％ (236/3860).The prevalence of different duration of low back pain (3 months,3-6 months,≥6 months) was 16.76％ (647/3860),4.12％ (159/3860) and 5.21％ (201/3860),respectively.The prevalence of females (28.83％) was higher than that of males (23.03％).The prevalence among different regions was significantly different.Prevalence in suburb and rural area (29.88％ and 27.54％,respectively) was higher than that in urban area (20.88％).No matter males or females,the prevalence in urban area was the lowest (17.48％ and 24.00％,respectively).With the increasing of age,the prevalence of low back pain became higher.In males,the prevalence of 55 to 59 years group was highest,while 60 to 64 years group was highest in females.In urban area and rural area,the prevalence of 60 to 64 years group was highest (34.43％ and 48.68％,respectively),while 55 to 59 years group was highest in suburb (47.26％).Conclusion The oneyear and point prevalence of low back pain among adults in Beijing are higher,with wide distribution.The chronic low back pain is more common.The prevalence of low back pain is higher in suburb and rural area.Females have a higher prevalence than males.Moreover,the prevalence of low back pain increases with age.

Objective To investigate the prevalence of cervical spondylosis among adults in Beijing.Methods The prevalence of cervical spondyiosis among people older than 18 years who had lived in Beijing for more than six months was investigated in December 2010.The multi-stage sampling was used in this cross-sectional study.The related information was obtained by a self-designed questionnaire.Single and muhivariable Logistic regression models were applied to analyze high risk districts and populations.Results A total of 3859 people were enrolled in this study.Among them there were 531 people with cervical spondylosis,and the prevalence was 13.76％.The prevalence of cervical spondylosis in suburb (15.97％) was the highest compared with city center and countrysides (x2=8.257,P=0.016).The prevalence in females (10.49％) was higher than that (16.51％) in males (x2=29.432,P＜0.001).The distribution of prevalence among different age populations was inverted U shape,and the prevalence was higher in 45 years group (20.43％) and 60 years group (18.91％) compared with other two groups.The prevalence in employed group was 1.303 times higher than that in unemployed group (95％CI:1.008,1.684).According to the muhivariable logistic regression result,suburb (OR=1.306,P=0.026),females (OR=1.81 1,P＜0.001),people who were 45years or 60 years (OR=2.171,P＜0.001; OR=2.430,P＜0.001,respectively) and employed and retired people (OR=1.664,P=0.001; OR=1.303,P=0.043,respectively) were high risk population of cervical spondylosis.Conclusion The prevalence of cervical spondylosis among adults in Beijing was much higher and vastly distributed.It's necessary to reinforce the prevention,diagnosis and treatment study.Young females,people aged 45 years or 60 years,and employed people were high risk populations.

Objective To investigate the protective effects of (Val8)GLP-1-Glu-PAL to dopaminergic neurons on PD mice induced by MPTP.Methods 56 male C57BL/6 mice which are 10-12 weeks old were randomly divided into Control,model (MPTP),muti-injection (Val8) and therapy (MPTP + Val8) groups by random number table method.Mice in MPTP group were received MPTP IP.treatment (30mg/kg · d),the control group were treated with 0.9％ saline with the same volume,Val8 group were injected with (Val8) GLP-1-Glu-PAL (25 nmol/kg),and MPTP+Val8 group were going to received (Val8)GLP-1-Glu-PAL 1 h after treatment of MPTP,all the groups were treated for 8 consecutive days.Behavior test were processed 2h after drug IP injection,including swimming test and rotarod test.Mice were sacrificed immediately 1h after the final trail of behavior test in 8th day,brains were withdraw for IHC assay which tested the number TH positive neurons in SNpc area.Results The classical PD behavior symptom were induced by MPTP,from 1 st to 8th,the swimming score(1.715±0.143 and dropping latency(68.048±7.823) were both decreasing compared with control.The differences were statistically significant (P＜0.05).The positive dopaminergic neurons were significantly lower in SNpc area (P＜0.01).Compared with MPTP group,(Val8) GLP-1-Glu-PAL could statistically improved the behavior deficit,the swimming score (1.120±0.143) and dropping latency(20.546±7.823) in the MPTP+Val8 group,and differences were statistically significant (P＜0.05).Meanwhile,the TH positive neurons were significantly elevated (P＜0.01).Conclusion The results suggest that (Val8)GLP-1-Glu-PAL has protective effects for MPTP induced mice PD dopaminergic neurons.