Objective To study the influence of pcDNA3.1-RhoC on the expression of endogenous angiogenic factors in HCC cells.Methods The reconstructed plasmid pcDNA3.1-RhoC was transfected into HepG2 cells, and expression of VEGF and bFGF was detected with the RT-PCR and immunohistochemical stain. HepG2 cells transfected with pcDNA3.1-RhoC or pcDNA3.1 were implanted into nude mice to observe the tumor occurrence rate.Results HepG2 cells transfected with pcDNA3.1/RhoC showed higher expression of RhoC . The expression of RhoC enhanced the expression of VEGF and bFGF(P

0 05). The levels of TC, LDL-C and apoB in the patients with CSX were significantly higher than those in the patients with CN(P

Objective The aim of this study was to elucidate the effects of homocysteine (Hcy) on the expression and secretion of tissue factor pathway inhibitor (TFPI) in endothelial cells (Ecs). Methods Cultured human umbilical vein endothelial cells (HUVECs) (ECV-304 strain) were incubated with homocysteine (Hcy) in different concentrations of 0, 25, 50, 100?mol/L, and 1,5,10mmol/L, respectively, for 24 hours. Cell viability was then determined by MTT assay, and lactate dehydrogenase (LDH) released into the culture medium was measured to assess cell damage. Antigen levels of the free form of TFPI after Hcy exposure were measured in culture media with ELISA (Diagnostica Stago, France). Furthermore, the total content of TFPI was assessed with cellular ELISA. Results Hcy in concentrations of 0～1mmol/L did not produce cell toxicity as shown by cell viability and LDH determination in culture media after 24 hours of incubation. When the endothelial cells were exposed to concentrations up to 5mmol/L, cell viability decreased, and a higher concentration of Hcy (10mmol/L) elicited a significant cytotoxic effect, as shown by a decreased cell viability and a higher amount of LDH in culture supernatants compared with the control cells. All the TFPI absorbance values in Hcy-treated cells and the free TFPI levels in culture supernatants were significantly increased, especially in the 50?mol/L group. Conclusion A lower concentration of Hcy did not show signs of cell toxicity but it could promote the expression and secretion of TFPI. The results can help us partly explain why the TFPI level was increased in some patients with hyperhomocysteinemia. They also suggested that Hcy could play an important role in the modulation of coagulation processes in blood circulation.

Objective To investigate the relationship between of the serum homocysteine (Hcy) level and the occurrence and severity of diseased coronary arteries in patients. Methods The total serum Hcy content was determined in sixty-seven patients with diseased coronary arteries and forty-three normal subjects. They were all subjected to coronary artery angiography for evaluation. Using the Gensini's scoring system the degree of severity of of pathology the coronary arteries were assessed. Other risk factors were analyzed in addition to Hcy and Gemini's scores. Results The total serum level of Hcy in patients with diseased coronary artery was significantly higher compared with the normal controls (P

Objective To establish an experimental model for exploring the role of RhoC gene in the invasiveness and metastasis of hepatocellular carcinoma.Method The RhoC gene was digested with restricted enzyme Hind III and XbaI,and direct cloned to pcDNA3.1.The recombinant vector (pcDNA3.1 RhoC) and the vector alone (pcDNA3.1) were transfected into HEPG2 cells with LIPFECTAMINETMReagent.After selected with hygromycin,resistant cloneies was obtained.The transcription and translation of RhoC gene were analysed with the reverse transcription PCR and immunohistochemical stain.Results The recombinant vectort (pc DNA3.1 Rhoc) express steadily in HerpG2 cells.Conclusions The modified tumor cells(HEPG2 RhoC) could be used to study the effect of RhoC protein on the invasiveness and metastasis of hepatocellular carcinoma.

Objective　To investigate the influence of γ-interferon (γ-IFN) on liver cancer cell line (Hep-G2). Methods　Observing the expression of Fas and Bcl-2 by γ-IFN-pretreated Hep-G2 cells via immunohistochemical stain; subsequently treating these cells with adrimysin, and observing the cell death rate and apoptosis of these cell by MTT and electroscopy. Results　(1) γ-IFN up-regulating the expression of Fas protein and down-regulating Bcl-2 protein (P<0.05), and the sensitivity of pretreated hep-G2 cell to adrimysin was increased. Conclusions　γ-IFN can rise the sensitivity of Hep-G2 to adrimysin via regulating the expression of Fas and Bcl-2.

Objective To investigate the influence of ?-interferon (?-IFN) on liver cancer cell line (Hep-G2). Methods Observing the expression of Fas and Bcl-2 by ?-IFN-pretreated Hep-G2 cells via immunohistochemical stain; subsequently treating these cells with adrimysin, and observing the cell death rate and apoptosis of these cell by MTT and electroscopy. Results (1) ?-IFN up-regulating the expression of Fas protein and down-regulating Bcl-2 protein (P

Objective To study the expression of RhoC mRNA in human primary hepatocellular carcinoma(PHCC) and paracarcinoma liver(PCL) tissues .Method Reverse transcription polymerase chain reaction(RT-PCR) was used to detect the expression of RhoC mRNA in the PHCC and PCL tissue of 30 patients with PHCC. Results The opacity density (OD)of RhoC mRNA expression in PHCC tissues was significantly higher than that in PCL tissures(P

Objective To investigate the distribution of ideal cardiovascular health status in Chinese health checkup population. Methods Subjects were enrolled from a health checkup population coming to the PLA health management center from Sept 2009 to Mar 2016. Modified with China's specifications, lifestyle and checkup data were collected and analyzed according to the American ideal cardiovascular health standard. Results A total of 37664 people were included in the study, of whom 72.88%were male and 27.12%were female. Comprehensive analysis showed that, among 7 health indicators including smoking, physical activity, diet, fasting blood glucose, blood pressure, total cholesterol and body mass index, there were only 43 subjects (0.11%) whose lifestyle reached the ideal cardiovascular health status, 11 subjects were in the poor cardiovascular health status, accounting for 0.03%. The rest of the subjects were in the intermediate levels of cardiovascular health status. There was a large gap between the daily diet intake and the dietary recommendation, and there was also a large gap between the actual level physical activity and the ideal level of physical activity recommended by related guidelines, indicating that unhealthy diet and inadequate physical activity are two bottleneck factors. Dairy product intake has the lowest satisfaction ratio, followed by vegetable and fruit intake. Most subjects (94.10%) showed insufficient physical activity. The percentage of three status of cardiovascular health among young, middle-aged and elderly subjects differed significantly (χ2=1200, P=0.000), and presented an increasing trend of ideal cardiovascular health and a declining trend of poor cardiovascular health status with age from youth to middle age, to the elderly, which reflected insufficient physical activity especially among the young people, and then the middle-aged. Meanwhile, the proportion of ideal cardiovascular health in men was higher than that in women. Conclusion The rate of ideal cardiovascular health is relatively low in the study population, unhealthy diet and inadequate physical activity are two bottleneck factors. Encouraging people to develop good eating and exercise habits might be the most effective method to improve population's ideal cardiovascular health status.

Objective To explore if there is a difference in blood pressure in left and right extremities.Methods A total of 20164 adults who took part in health check-up at Health Examination Center of Chinese PLA General Hospital between December 2009 and August 2011were enrolled in this study.Age,height and body weight were recorded,and blood pressure in extremities was measured in synchronous way by using an arteriosclerosis detector.Results (1) Blood pressure in upper left extremity was slightly higher than that in upper and lower right extremity ( all P =0.0001).( 2 ) Difference in diastolic blood pressure in upper left and right limbs in females (2.1±6.7) mm Hg(1mm Hg =0.133 kPa) was more significant than that in males (1.4 +6.5 ) mm Hg ( P =0.0000).In males,difference in systolic blood pressure between two lower extremities ( 2.3 ± 9.6 )mm Hg was more significant than that in females (1.9 ±13.4) mm Hg ( P =0.0225 ).( 3 ) The above mentioned differences were found in low or normal weight and over-weight/obesity populations,which was not correlated with body mess index.(4) The difference of diastolic blood pressure in left and right limbs of relatively taller adults ( ＞170 cm) was more significant than that in shorter populations ( ＜170 cm ) (P =0.001).Conclusion The differences in blood pressure in left and right extremities do exist.