LATS2-gene,as one homolog of LATS tumor suppressor family,plays an important role in the control of cell cycles and tumor development through Hippo pathway,p53 gene and Ras-ERK signal transduction.Once an aberrant expression of LATS2 in cell to happen,it may lead to the tumor occurrence.

Objective To explore the clinical characteristics of B cell prolymphocytic leukemia (B-PLL).Methods The clinical manifestation,treatment and outcome of a 33-year-old man with B-PLL were reported.Results The young patient had thrombocytopenia and systemic B-symptoms,markedly raised lymphocyte'count,bulky splenomegaly and lymphadenopathy.He refused stem cell transplant,so RFC regimen (fludarabine,cyclophosphomide and rituximab) therapy were administered.After 4 cycles of RFC therapy,the patient achieved a complete immunophenotypical and hematological remission response.Conclusion RFC therapy might be a feasible and useful treatment option for B-PLL.

Objective To investigate the effect of Site-Rite 5 vascular ultrasound on puncturing of grade Ⅱ and Ⅲ veins.Methods Purposive sampling was used and 59 patients with grade Ⅱ and iⅢ veins to receive peripheral intravenous catheteration (PIV) were divided into observation group and control group according to the different wards:30 patients in the observation group and 29 in the control group.The control group received indwelling PIV by routine method and in the observation group,the patients' veins were assessed by Site-Rite 5 ultrasound-guided instrument and marked,followed by puncturing with PIV.The two groups were compared in terms of one-time success rate and incidence of complications.Results The one-time success rate of the observation group was significantly higher than that of the control group.There was no significant difference in the incidence of complications between the two groups (P ＞0.05).Conclusions By the Site-Rite 5 ultrasound-guided instrument,the invisible veins become visible.It is beneficial to improve the success rate of puncturing.

Objective To develop a dental crown remover with the strength provided constantly and adjusted freely.Methods The remover was composed of a shell, a crown hook, a conduction mechanism, a strength generating mechanism and a percussion mechanism. The spring had the explosive force formed in the crown head to destruct the cohesion of the cement.Results The remover could remove the crown, with one hand freed for the protection of abutment and mucous membrane.Conclusion The remover has simple structure and easy operation, and is worth popularizing clinically.

Objective:This study aimed to compare the clinical efficacy and prognosis between rituximab plus fludarabine and cyclophosphamide (FCR) and fludarabine and cyclophosphamide (FC) regimens for patients with chronic lymphocytic leukemia (CLL). Methods:The clinical data of 58 patients with CLL treated with FCR or FC regimens from December 2002 to January 2012 were analyzed retrospectively. Therapy efficacy and prognosis were compared between the two groups. Results:Among the 58 pa-tients, 27 (44.4%) experienced complete remission (CR) in the FCR group and 31 patients (19.4%) experienced CR in the FC group (P=0.039). The overall response rate (ORR) of the FCR group was higher than that of the FC group (81.5%and 51.6%, respectively, P=0.017). Fourteen patients achieved MRD-negative rating after therapy. PFS and OS in MRD-negative patients were superior compared with the MRD-positive group (P=0.000, 0.003). The proportion of MRD-negative patients in the FCR group was higher than that in the FC group (37.0%and 12.9%, respectively, P=0.032). PFS in high-risk genetic patients was lower than that in low-risk genetic patients (P=0.011, 0.027). The OS time between the two groups did not exhibit any difference. Conclusion:FCR produced a high CR and ORR in patients with CLL. Many patients in the FCR group were responsive to the treatment. Thus, FCR could be a more effective regimen than FC for patients with CLL.

Objective: To investigated the effect of long-term low-concentration mixed benzene exposure on peripheral blood of male workers. Methods: A case-control study was conducted to select 452 male workers exposed to mixed benzene (benzene, toluene, xylene) for five consecutive years from January 2012 to December 2016 in an automobile manufacturer as case group, and 438 male administrative and logistic managers who underwent physical examination during the same period as control group. The peripheral blood of the two groups was tested and compared, and the occupational hazards in the workplace were detected. Results: There were low dose exposure to mixed benzene in the enterprise, but the test results met the occupational exposure limit requirements. During the five years from 2010 to 2016, between the two groups of workers, the mean values of WBC, NEUT, RBC and Hb were statistically different (P<0.05). There was no statistical difference (P>0.05) in the mean value of PLT. The abnormal rate of main peripheral blood indexes in the control group was higher than that in the exposed group. There were significant differences in NEUT, RBC and Hb (P< 0.05), but no significant differences in WBC and PLT (P>0.05). Conclusion It can not be concluded that long-term low-concentration mixed benzene exposure can cause the change of peripheral blood index.

Objective To develop the female breast cancer patients' needs assessment scale and test its reliability and validity.Methods Based on extensive literature and Maslow model, the initial assessment scale of female breast cancer patients' needs was established by literature analysis, qualitative interviews and experts consultation. The method of convenience sampling was used to conduct the pilot survey, and evaluate its reliability and validity.Results The initial needs assessment scale of breast cancer patients consists of 37 items. Through item discrimination, correlation analysis, Cronbach's α, factor analysis, 7 items were deleted, and the 30 items scale was eventually formed and consists of 5 dimensions, including physiological needs, safety needs, love and belonging needs, esteem needs and self-actualization needs. The overall Cronbach's α and split half reliability were 0.952 and 0.856 respectively. The content validity ratio (CVI) was 0.99. Construct validity was analyzed by the method of factor analysis. The 5 common factors extracted by orthogonal rotation with principal component method showed consistency with the theoretical conception. The results showed good reliability and validity of the scale.Conclusions Based on Maslow's hierarchy of needs theory, the female breast cancer patients' needs assessment scale has good reliability and validity, and can be used to evaluate the needs of breast cancer patients.

Objective To observe the effect of removal alcohol with iodine or not in PICC maintenance . Methods Sixty patients who had PICC were randomized into observation group ( n =30 ) and control group ( n=30 ) .Besides routine disinfection method and operation , the observation group used alcohol to remove the iodine.While the control group only received the routine disinfection method and operation .The bacterial cultivation of the skin surface around the PICC insertion site , drying time and time to change the dressing were compared between groups , and observed rolled-up dressing after 7 days of change the dressing .Results The bacterial cultivation of the skin surface around the PICC insertion site were not significantly different between groups (t=6.38,P >0.05).The natural air drying time and the total time of change the dressing of the observation group were (6.41 ±4.80) minutes and (16.60 ±2.71) minutes, which were significantly shorter than those of the control group (t =6.38,2.89, respectively;P <0.05).And the rolled-up dressing was 93.3%in the control group and 10.0%in the observation group .The difference was significant (χ2 =41.72, P<0.05).Conclusions Using alcohol to remove the iodine in PICC maintenance can shorten the nursing time and improve the work efficiency .It can also decrease the rolled-up dressing, reduce the rate of infection of the insertion site and increase the safety of PICC indwelling .What's more, iodine on the skin removed with alcohol makes the patient feel much more comfortable .

Objective To optimize the prescription and preparation technology of finasteride dispersible tablets .Methods Wet granulation technique was applied to optimize the formulation and technology of finasteride tablets .Results The formulation of fi-nasteride tablets was that microcrystalline cellulose and lactose acts as diluent , 5% of low-substituted hydroxypropyl cellulose acts as disintegrant , 5%of povidone in alcohol water mixture acts as adhesive and 15%of opadry 85 G type acts as coating solution .The per-centage of dissolution was more than 90%in 45 miniutes.Conclusion The self-made finasteride tablets had stable quality , reliable process and were suitable for industrialized mass production .

Objective To investigate the effect of human caring ability on job satisfaction of nurses in the oncological department. Method About 215 nurses in the department were enrolled in the study with both caring nurse-patient action scale (CNPI-23) and Minnesota satisfaction questionnaire (MSQ). Results CNPI-23 score on oncology nurses was 88.42±13.54 and MSQ score 44.87±11.34. Nurses' caring ability was associated with age, work experience, marital status and job title. Nurses' humanistic care ability was positively correlated with job satisfaction (r=.433;P<0.001). Job satisfaction was positively related with humanistic care ability (t=6.617, P<0.001), which explained 23.4% variable. Conclusions The oncological nurses' caring ability needs to be improved. It is possible to improve the capability of nurse humanity from the perspective of job satisfaction.