This study revealed that the content of intra and extracellular polysaccharides may be related to the cultural conditions.With decreasing NaHCO 3 or NaNO 3 concentration,the content of intracellular polysaccharide was reduced,Reduction of NaNO 3 concentration still caused the rise of extracellular polysaccharides.The K 2HPO 4 or NaCl concentration also had an effect on the production of both intracellular and extracellular polysaccharides.When PH value of media maintained at 8.0,there was the highest content of intra and extracellular polysaccharides.The same high content also occurred after culture of nine days.In addition the content of intracellular polysaccharide was higher under continuous light than under the light/dark circle.

[Objective]To analyse the operative complications in total hip arthroplasty.[Method]Sixty-two patients (62 hips) undergone total hip arthroplasty from March 1998 to November2009,were grouped according to the different causes of the operative complications.[Result]Based on the analysis of all cases,it was found that the most common causes resulting in the failure of the surgery were surgical technichque and fracture,anesthesia,osteoporosis,diabetes mellitus,and cerebral hemorrhage.Complications in all 5 cases were sudden death in 1,femural fracture in 1,unequal-length lower timbs in 1,and postoperative dislocation in 2.[Conclusion]Total hip arthroplasty has been extensively used as an effective procedure for the reconstruction of hip joint.Long-term excellent clinical results are related to preoperative preparation,patient matched fact,and the experience and surgical technique of the surgeon are also of importance to the final outcome.

Objective To investigate the effect of Salvia miltiorrhiza injection on proliferation , apoptosis and expression of hydroxyproline in human embryonic lung fibroblast cells. Methods TGF-β1 was administered to induce the proliferation in human embryonic lung fibroblast cells; the effect of Salvia miltiorrhiza injection on the number of human embryonic lung fibroblast cells was detected by MTT method; ki67 expression by immunocytochemical method;cell apoptosis by flow cytometry and the expression of hydroxyproline by colorimetry method. Results TGF-β1(0, 2.5, 5, 10, 20, 40 μg/L)could up-regulate cell number of human embryonic lung fibroblast cells in a dose-dependent manner , while the OD value of human embryo lung fibroblasts cells declined pretreated with Salvia miltiorrhiza injection in a dose-dependent manner and Salvia miltiorrhiza injection could induce the apoptosis and down regulated hydroxyproline expression in human embryo lung fibroblasts cells. The results of flow cytometry indicated that cell apoptosis increased after treated with Salvia miltiorrhiza injection when compared with TGF-β1 group (P < 0.05). Meanwhile, Salvia miltiorrhiza injection could down regulate the expression of hydroxyproline (P < 0.01). Conclusions Salvia miltiorrhiza injection can target human embryonic lung fibroblast cells , play a potent role in the airway remodeling through the promotion of its apoptosis and down regulate the expression of hydroxyproline.

This paper observed the influence of water-soluble Chitosan on the growth of bacteria cultured in vitro with solid medium. The results showed that Escherichia coli、staphylo- coccus aureus、Bacillus subtillus、Sarcina and Actinomyces5406 could be inhibited to different degrees by Chitosan with certain concertrations. The corresponding MIC Were 0. 5%、0. 3%、 0. 5%、0. 3% and 0. 5%. The results also showed that Chitosan had stable antibacterial action after being treated with heat and it could keep instant flavouring kelp fresh and antiseptic.

Objective To investigate the correlative factors that affect the outcome of pregnancy complicated with burn injuries and to provide the treatment proposal for the pregnant women with vary gestational age. Methods Retrospective analysis of 13 cases of burn injuries during pregnancy was made. Results All patients required standard treatment to heal the burn injuries. During the first trimester, abortions occurred in two of the four patients; Abortions occurred in two and premature labour occurred in one of the nine patients who were injured during their second or third trimester. Conclusion Abortions or premature labour appeared during pregnancy as complications of the treatment of burn injuries. Enough fluid therapy in time in early phase of burn is necessary for mother survival and placental perfusion.

Purpose:To investigate the relationship of expression of CD44v6, E cadherin (E Cad) with clinic biologicl behavior in breast cancer and its correlation. Methods:Catalyzed signal amplification immunohistochemical method was used to detect the expression of CD44v6 and E Cad in 94 cases of breast cancer.Results:The positive expression of CD44v6 and the negative expression of E Cad were positively correlated with the grading, clinical stage, lymph node metastasis recurrence and prognosis of breast cancer. In breast cancer, the expression of CD44v6 had negative correlation to that of E Cad.Conclusions:Several genes were involved in the occurrence and development of breast cancer in which the expression of CD44v6 had negative regulative synergistic action with that of E Cad. CD44v6 and E Cad expression, as a clinical valuable marker, has a great significance in assessing node metastasis and survival.

Objective:In order to study the correlation between The expression of CD44v6 protein and Bcl-2 protein in thyroid cancer were examined.Methods:The expression of CD44v6 and Bcl-2 in 50 thyroid cancers,20 adjacent noncancerous portion,45 adenoma and 10 normal thyroid tissue were respecitively investigated by catalyzed signal amplification immunohistochemical technique. Results:The positive rate of CD44v6 and Bcl-2 in thyroid cancer was 64.0% and 46.0%, which was significantly higher than that in adenoma or adjacent noncancerous ( P

Objective:To observe the changes of 5-HT1B and D1DR expression in nucleus of SNr/GPi-VL-SMA pathway in pre and post-exhaustive exercise,and investigate the central mechanism of SNr/GPi-VL-SMA pathway modulating the occurrence and development of exercise-induced fatigue.Method:Twenty-four male Wistar rats were randomly divided into control group (CG),immediately after exhaustion group (EG),and 90min after recovery group (RG),each consists of 8 rats.The expression level of 5-HT1B and D1DR receptors in each group were observed with the immunohistochemistry technique.Result:Compared with CG,the positive cell area/scanning area value (R value) and the average optical density value (AOD value) of 5-HT1B receptor in SNr and GPi of the EG and RG rats both decreased significantly (P ＜ 0.05),while the R value and AOD value of D1DR both increased significantly(P＜ 0.05).The R value and AOD value of 5-HT1B receptor in VL of EG and RG rats increased significantly(P＜0.05,P＜0.01).In SMA,the R value and AOD value of 5-HT1B receptor of EG and RG rats increased significantly (P＜ 0.05),while,the R value and AOD value of D1DR receptor decreased significantly (P ＜ 0.05).Conclusion:Changes of 5-HT1B receptor and D1DR receptor expression in the nuclei of ‘SNr/GPi-VL-SMA'pathway caused the changes of the neurotransmitters release,thus affecting the excitability of the electrical activity of neurons,this might be one of the important ways for ‘SNr/GPi-VL-SMA'pathway modulating the exercise-induced fatigue and exercise performance.

Objective To determine whether tissue factor(TF) is present in the amniotic fluid of normal term pregnancy. Methods Tissue factor antigen levels in amniotic fluid, blood plasma, centrifugal supernatant and sediment of 16 normal term pregnancies were detected with immunoenzymatic method(ELISA). Results Tissue factor antigen levels in the blood plasma, the whole amniotic fluid, the centrifugal supernatant and the sediment were [(31?9) ng/L0, [(404?186) ng/L], [(348?177) ng/L] and [(1360?639) ng/L], respectively. Tissue factor antigen concentrations in three states of the amniotic sediment(P

Objective To explore the detection rate and major risk factors of nodular thyroid diseases among adults.Methods A total of 35 495 adults who carried out health checkup during January 1st,2009 and December 31th,2010 in our center were assessed for the occurance of nodular thyroid diseases and the size and number of thyroid nodules by age and gender.Chi-square test,rank sum test and Chi-square trend test were used for data analysis.Results The total detection rate of nodular thyroid diseases was 33.87％ (female vs male:41.76％ vs 29.68％).The prevalence of single thyroid nodule was decreased with age,as opposed to multi-thyroid nodules.Single thyroid nodule was more commonly seen in males,which was reversed in multi-thyroid nodules.Small,middle and large thyroid nodules were found in 77.49％,18.19％ and 4.32％ males or 72.57％,21.59％ and 5.84％ females,respectively.Moreover,thyroid cancer was detected in 0.4‰ of those with nodular thyroid diseases.Conclusions The detection rate of nodular thyroid diseases among adult health checkup receivers was higher,which calls on the needs of intensive monitor and effective prevention of the condition.