Objective To observe the changes of serum endotoxin and interleukin-6 level before and after treatment at intestine get rid of filth and adjustment intestine microenviroment in patients with advanced stage liver cirrhosis and to oberserve the incidence of spontaneous bacterial peritonitis(SBP) and to research a method that can more efficient reduce the incidence of SBP.Methods Group A was given conventional treatment such as protection liver,supplement human albumin and uragogue;goup B was given FPA 0 2g tid po,continuous three days on the basis of two weeks conventional treatment,group C was given Tab comfort get 0 5g tid po,Changtai orally taken liquid,10ml tid po,lactulose 10ml tid po, for all 8 weeks,on the basis of group B treatment after pausing use FPA.Results Serum endotoxin and interleukin-6 levels in group A was not significantly changes after treatment,,SBP incidence was higher than both group B and group C;at group B,serum endotoxin and interleukin-6 levels decline,SBP incidence significantly drop afer treatment,at group C,serum endotoxin,interleukin-6 level and SBP incidence significantly drop,the difference was significant at statistics as compared with group A and B.Conclusions The treatment at intestine get rid of filth and adjustment intestine microenviroment can effectively reduce serum endotoxin and interleukin-6 levels and can significantly reduce the incidence of SBP.

Objective To investigate the dynamic expression of nitric oxide synthase(NOS) in the apoptosis of primary cultured rat cortical neruons following hypoxia/reoxygenation(H/R) and the protective role of extract of ginkgo biloba(EGB). Methods The cortical neurons of E16-17 days fetal rat were primarily cultured.The apoptosis model of primary cultured cortical nurons following H/R was established by using W-G staning,electromicroscopy,TUNEL staining.The dynamic expression of NOS different H/R times was investigated with NADPH-diaphorase histochemical method. Results H/R can cause apoptosis of primary cultured rat cortical neurons.In the experiment of H-2R-0,H-4R-0, H-6R-0,H-8R-0 and H-2R 18,H-4R 18,H-6R 18 H-8R 18,the apoptosis cells occurred after 4 hour hypoxia.The increasing of apoptosis cell acted as time-dependence and the peak value was at H-8R 18.The expression of NOS increased both after 2 hour hypoxia and reoxygenation 18 hour after 8 hour hypoxia compared with the normal control group.EGB could inhibit the increasing and decrease the percentage of apoptosis.Conclusion The apoptosis of primary cultured rat cortical neurons could be induced by H/R.The increasing of NO might be one of the mechannisms of apoptosis.EGB could singnificantly inhibit the apoptosis by means of inhibiting the expression of NOS and reducing the production of NO.;

OBJECTIVETo compare the clinical effect of the double eyelid blepharoplasty with central minimal incision and with three minimal incisions. Methods: From Jul. 2010 to May 2012, 268 cases (Group A) received double eyelid blepharoplasty with central minimal incision, while 102 cases (Group B) underwent double eyelid blepharoplasty with three minimal incisions. Photos were taken immediately, and 1,2, 4, 8,12 weeks after operation. Operation time, recovery time and postoperative complications were evaluated and recorded. The operation time and recovery time were analyzed by Wilcoxon rank sum test. The postoperative complications were analyzed by chi square test. The satisfactory degree was analyzed by t test.

RESULTSThe operation time in Group A was (25.63 ± 3.74) min, compared with that (29.90 ± 4.13) min in Group B (Z = -8.011, P <0.01). Meanwhile, the recovery time in Group A was shorter than that in Group B (Z = -15.887, P <0.01). The occurrence rate of postoperative complications,including hematoma,recurrence and scar hyperplasia in Group A was also lower than that in Group B. At the same time, the satisfactory degree in Group A was(97.302 ± 1.764), which was higher than that(88.628 10.880) in Group B (t = 12.650, P <0.05).

CONCLUSIONSThe double eyelid blepharoplasty with central minimal incision, which is suitable for all cases except those who has serious blepharochalsais, has more advantages than double eyelid blepharoplasty with three minimal incisions.

Blepharoplasty ; adverse effects ; methods ; Cicatrix ; pathology ; Eyelids ; surgery ; Hematoma ; Humans ; Hyperplasia ; Photography ; Postoperative Complications ; Prospective Studies ; Recurrence

OBJECTIVETo investigate the effect of light intensity on growth and photosynthetic of Chrysanthemum morifolium.

METHODThe growth characteristics of C. morifolium were measured under different treatments (100%, 80%, 60%, 40% and 20% of full sunlight). The photosynthetic characteristics and chlorophyll fluorescence parameters of leaves under different light intensity were determined by a LI-6400 photosynthesis system and a PAM-2100 chlorophyll fluorescence system.

RESULTWith the reduction of irradiance, the diameter of the stem reduced, plant height, leaf length, leaf width and length/width raised, assimilation product increased; Content of photosynthetic pigment increased between light intensity 100%-40% reduced under 20% treatment, chlorophyll a/b decreased. Light compensation point (LCP), apparent quantum yield (AQY) increased first and reduced later, photosynthesis rate (P(n)), stomatal conductance (G(s)), intercellular CO2 concentration (C(i)) and transpiration rate (T(r)) decreased, stomatal limitation value (L(s)) rose. Chlorophyll fluorescence parameters F(v)/F(m) increased, phiPS II, F(v)'/F(m)', ETR and qP increased between irradiance 100%-60%, NPQ decreased, such as phiPS II decreased and NPQ increased when irradiance was lower than 40%.

CONCLUSIONWeak light condition was unfavorable to the growth of C. morifolium and the light conditions of culture should be control between 80%-60% of full sunlight.

Biomass ; Chrysanthemum ; growth & development ; physiology ; radiation effects ; Photosynthesis ; radiation effects

Objective:To observe modulation of gut functions and intestinal mucosal immune barrier by ShengJiangXieXinTang in rats receiving Irinotecan(CPT-11).Methods:Sprague Dawley male rats(n=18)were randomly assigned to three groups:(1)herb group (using ShengJiangXieXinTang by oral administration once a day from day 1 to day 9 and being injected with 150 mg/kg?d CPT-11 on day 4 and 5 into the tail vein); (2)diarrhea control group: using distilled water instead of ShengJiangXieXinTang,with the same treatment of CPT-11 as the herb group; (3)normal control group: using normal saline instead of CPT-11, distilled water instead of ShengJiangXieXinTang,and with the same treatment of as the CPT-11 herb group.The animals were scored in terms of delayed-onset of diarrhea. Rats were killed on day 10,collecting ileum,cecum and colon for pathological examination.The damages in intestinal mucosa were assessed under light microscope according to the criterion of chiu's score.CD4+,CD8+T-lymphocytes and SIgA were enumerated by immunohistochemical staining and calculated by imaging analyzer.Results:Compared with diarrhea control group,the incidence of diarrhea and the damage in intestinal mucosa of the herb group was milder(P

ObjectiveTo characterize the neural progenitor cell in the human amnion mesenchyme and epithelial layer with specific mark proteins of neural stem cell.MethodsExpressions of specific mark proteins of neural stem cell including nestin, glial fibrillary acidic protein (GFAP), musashi-1, vimentin and PSA-NCAM in human amnion tissue and cultured amniotic cells were determined by immunohistochemistry and immunofluorescence staining.ResultsExpressions of pluripotent neural stem cell specific makers (nestin, musashi-1, vimentin and PSA-NCAM) were detected in the human amnion mesenchyme and epithelial layer. In addition, cultured amniotic cells were expressed several neural stem cell specific markers including nestin, GFAP and PSA-NCAM. Nestin+ and GFAP+ double positive cells were identified in the human amnion tissue and cultured amniotic cells by immunohistochemistry and immunofluorescence staining.ConclusionSpecific mark proteins of neural stem cell are expressed in human amnion tissue and cultured amniotic cells.

OBJECTIVETo develop a RP-HPLC method for the determination of quercetin, luteolin, apigenin and acacetin in Flos Chrysanthemi indici.

METHODAn Eclipse XDB-C18 column (4.6 mm x 250 mm, 5 microm) was used at 25 degrees C with the mobile phases of methanol-0.2% phosphatic acid in a gradient manner. The flow rate was set at 1.0 mL x min(-1). The detection wavelength was 350 nm.

RESULTThe linear response ranged from 1.02-20.48 mg x L(-1) for quercetin (r = 0.9994, n = 5), 1.03-20.54 mg x L(-1) for luteolin (r = 0.9992, n = 5), 1.12-22.40 mg x L(-1) for apigenin (r = 0.9995, n = 5), 1.01-20.22 mg x L(-1) for acacetin (r = 0.9998, n = 5), respectively. Recoveries were 101.3% with RSD 1.3% for quercetin, 100.62% with RSD 1.4% for luteolin, 98.42% with RSD 1.7% for apigenin and 99.02% with RSD 0.8% for acacetin. A significant difference (alpha = 0.01) among the contents of four flavonoids and total flavonoids was found.

CONCLUSIONThe method is quick, simple and repeatable for simultaneous determination of quercetin, luteolin, apigenin and acacetin in Flos Chrysanthemi Indici.

Apigenin ; analysis ; Chromatography, High Pressure Liquid ; methods ; Chromatography, Reverse-Phase ; methods ; Chrysanthemum ; chemistry ; Flavones ; analysis ; Flavonoids ; analysis ; Flowers ; chemistry ; Luteolin ; analysis ; Plant Extracts ; analysis ; Quercetin ; analysis

OBJECTIVETo study the effects of soil factors on the active component content of Chrysanthemum morifolium and screen out the leading factors.

METHODThe active component of water soluble extracts, flavonoids, phenolic compounds and mineral elements were determined and chemical properties and mineral elements of soil were analyzed for studying the effects on Ch. morifolium through correlation, stepwise regression, path and grey correlation analysis.

RESULTSoil available P and K were the most important factors that affected the active component content of Ch. morifolium, followed by urease, phosphatase and invertase activities and organic matter. The mineral elements in Ch. morifolium and in soil correlated well, P and K were enriched in the plant mostly, followed by Cd, Ca, Zn, Cu. The main leading factors of mineral elements in soil were P and K, followed by Fe, Cu and Zn.

CONCLUSIONSoil was one of the important factors which affected the active component content of Ch. morifolium.

Agriculture ; methods ; Chrysanthemum ; chemistry ; drug effects ; metabolism ; Ecosystem ; Environmental Monitoring ; methods ; Minerals ; pharmacology ; Pleasure ; Potassium ; pharmacology ; Soil ; analysis ; Soil Pollutants ; pharmacology ; Water ; physiology

OBJECTIVETo study the effect of light intensity on physiological and biochemical characteristics of Chrysanthemum morifolium at the vegetative stage.

METHODThe dynamic response of physiological and biochemical indexes of Ch. morifolium were measured under different treatments (100%, 80%, 60%, 40% and 20% of the full sunlight) at the vegetative stage.

RESULTThe physiological and biochemical indexes of Ch. morifolium showed dynamic changes with the progress of growth and the increase of the treatment time. The soluble sugar content decreased when the light intensity reduced, and had a significant positive correlation with the light intensity. Soluble protein content rose firstly and fell later, malondialdehyde content increased, superoxide dismutase and catalase activity decreased initially and increased afterwards.

CONCLUSIONProper shading benefits the nitrogen accumulation of Ch. morifolium at the vegetative stage, and reduces the strength of stress condition. The suitable light environment for growth of Ch. morifolium at the vegetative stage is about 80%-60% of full sunlight and the optimum treatment time is 20-40 days.

Catalase ; metabolism ; Chrysanthemum ; physiology ; Light ; Lipid Peroxidation ; Superoxide Dismutase ; metabolism

Objective: To explore mediation of emotional regulation in the association between aggression behavior and non-suicide self-injury among rural middle school students. Methods: A multi-stage stratified random cluster sample of 14 291 middle school students from 15 cities in five provinces of China (Anhui, Yunnan, Guangdong, Heilongjiang, Hubei). A structured questionnaire was used to assess non-suicide self-injury, emotional regulation and aggressive. Results: During the past year, about 28.51% of participates reported self-injury, with 13.39% of them had 5 times or more. The five dimensions of aggressive behavior were positively correlated with non-suicide self-injury(P<0.01), emotional regulation was negatively correlated with non-suicide self-injury(P<0.01). The mediating effect of emotional regulation between aggression (physical aggression, verbal aggression, indirect aggression, anger and hostility) and non-suicide self-injury was found significant, the ratio of whose were 25.93%, 22.73%, 19.61%, 27.55% and 18.80%, respectively. Conclusion Emotional regulation plays a mediating role in adolescent self-injury and aggression, indicating self-injury and aggression could be prevented by enhancing emotional regulation among adolescents.