OBJECTIVE To analyze and compare the value of c-reactive protein(CRP) and body temperature in the infection diagnosis and severity of infection among the patients with malignant hematological disease.METHODS According to the microorganism detection and application of antibiotics,we divided the 119 patients into infection group and noninfection group from May 2004 to May 2005 in our ward.CRP and temperature of the patients were measured and.RESULTS There were 88 cases in the infection group and 31 cases in the noninfection group.The CRP plasma concentration had significant difference between too groups(P

Objective This study is about to detect the KCNJ5 gene variations in aldosterone-producing adenoma (APA) with primary hyperaldosteronism (PA),and to investigate the association of the KCNJ5 gene missense mutations with APA and PA.Methods A total of 46 APA tumors and their clinical characteristics were collected from Hypertension Center of the People's Hospital of Xinjiang Uygur Autonomous Region,and all the tumors were confirmed by pathology.All the samples of the coding region segments of KCNJ5 were detected by PCR and direct DNA sequencing to compare the different missense mutations in the tumor cells and peripheral blood cells and to analyze the association between the genotype and phenotype.Results Three missense mutations were found in 46 patients with APA:c.451G ＞ C/A (p.G151R) (5/46),c.503T ＞ G (p.L168R) (4/46),c.830T ＞ A (p.S209T) (12/46).S209T,as an unreported somatic mutation was observed.There were no missense mutations detected in the peripheral blood.Sex,age,systolic blood pressure,diastolic blood pressure,duration of hypertension,plasma potassium,urine potassium,aldosterone,plasma renin activity,aldosterone,and plasma renin activity ratio,as well as the rate of positive family history were compared between the mutants and wild-types.Systolic blood pressure and plasma potassium level among G151 R,L168R,and S209T had statistical differences.Systolic blood pressure in G151R was significantly higher than the other two groups,while the plasma potassium level was significantly lower than the other groups.Conclusions Three missense mutations were found in 46 patients with APA.The subjects with mutations had more serious condition than those without mutations after comparing their clinical phenotype.Besides,other different subtypes may exist in PA.

Objective To systematically sort out the interventional studies on the application of auricular point sticking technique in patients with urinary calculi by means of evidence map analysis in order to understand the distribution of evidence in this field and the problems of the studies.Meth-ods Chinese and English databases such as CNKI,Wanfang,VIP,China Biology Medicine(CBM)Database,PubMed,Cochrane Library,CINAHL complete,Web of Science,Elsevier Science Direct,Embase,etc.were systematically searched to screen for studies related to the application of auricular point sticking technique in patients with urinary calculi.The time frame of the review was from the database construction to June 2024,and the trend of publication,type of literature,sample size in the literature,interventions,distribution of acupuncture points,outcome indexes,and methodological quality of the literature were analyzed by using graphs and text.Results A total of 79 papers were included,including 65 randomized controlled studies,5 quasi-experimental study,and 9 studies with unclear grouping basis.The overall trend of the number of publications was increasing,and the aver-age sample size was 103.37 patients.The most frequently selected auricular acupuncture points were kidney,ureter,sympathetic point,Shenmen,and bladder,etc.,and the endpoint indicators of greater concern were clinical efficacy,pain relief,and stone expulsion,etc.The methodological quality of the literature was not high.Conclusion Auricular point sticking technique has certain ad-vantages in the treatment of urinary calculi,and the selection of acupoints and outcome indicators are diverse.However,most studies have shortcomings in scientific research design,personalized intervention measures,measurement and analysis of outcome indicators,etc.Therefore,more scientific and rigorous large-scale,multi-center randomized controlled studies are needed in the future to provide high-quality evidence-based basis for the application of auricular point sticking technique in patients with urinary calculi.

Objective To systematically sort out the interventional studies on the application of auricular point sticking technique in patients with urinary calculi by means of evidence map analysis in order to understand the distribution of evidence in this field and the problems of the studies.Meth-ods Chinese and English databases such as CNKI,Wanfang,VIP,China Biology Medicine(CBM)Database,PubMed,Cochrane Library,CINAHL complete,Web of Science,Elsevier Science Direct,Embase,etc.were systematically searched to screen for studies related to the application of auricular point sticking technique in patients with urinary calculi.The time frame of the review was from the database construction to June 2024,and the trend of publication,type of literature,sample size in the literature,interventions,distribution of acupuncture points,outcome indexes,and methodological quality of the literature were analyzed by using graphs and text.Results A total of 79 papers were included,including 65 randomized controlled studies,5 quasi-experimental study,and 9 studies with unclear grouping basis.The overall trend of the number of publications was increasing,and the aver-age sample size was 103.37 patients.The most frequently selected auricular acupuncture points were kidney,ureter,sympathetic point,Shenmen,and bladder,etc.,and the endpoint indicators of greater concern were clinical efficacy,pain relief,and stone expulsion,etc.The methodological quality of the literature was not high.Conclusion Auricular point sticking technique has certain ad-vantages in the treatment of urinary calculi,and the selection of acupoints and outcome indicators are diverse.However,most studies have shortcomings in scientific research design,personalized intervention measures,measurement and analysis of outcome indicators,etc.Therefore,more scientific and rigorous large-scale,multi-center randomized controlled studies are needed in the future to provide high-quality evidence-based basis for the application of auricular point sticking technique in patients with urinary calculi.

Objective:To search, evaluate and summarize the best evidence for oral fluid intake management in adult patients with urinary calculi, providing a decision-making basis for clinical nursing.Methods:Using keywords such as urolithiasis, urinary calculi, fluid intake, water intake, fluid, etc., a systematic search was conducted in relevant domestic and international databases, guideline websites, and professional association sites for evidence related to oral fluid intake management in adult urolithiasis patients. This included clinical decisions, best practices, guidelines, expert consensus, evidence summaries, systematic reviews, and recommended practices, covering the period from January 1, 2018 to June 1, 2023. Two researchers trained in evidence-based nursing independently evaluated the quality of the literature and extracted and integrated the evidence.Results:A total of 23 articles were included, comprising two clinical decisions, one best practice, four guidelines, 13 systematic reviews, and three recommended practices. Ultimately, 18 pieces of best evidence were summarized from six aspects: the importance of fluid intake, intake volume, timing of intake, the relationship between different types of fluids and the risk of urinary calculi, intake recommendations, monitoring methods, and precautions.Conclusions:This study summarizes the best evidence for oral fluid intake management in adult patients with urinary calculi, which can provide reference for clinical nursing. When applying this evidence, it is essential to fully consider clinical circumstances and patient characteristics to reduce the recurrence rate of urinary calculi.

OBJECTIVETo investigate the prevalence of KCNJ5 gene missense mutations and their role in patients with unilateral adrenal hyperplasia (UAH).

METHODSFourteen UAH tissues were collected through surgical resection, and all the tissues were confirmed by pathology. Peripheral blood samples of the same patients were collected as control. The coding regions of the KCNJ5 were detected by direct DNA sequencing. Protein structure and function were predicted with specific software.

RESULTSThree missense mutations were detected among the 14 patients with UAH, which included c.451G>C/A (p.G151R) (2/14), c.503T>G (p.L168R) (1/14), c.830T>A (p.S209T) (9/14). Among these, c.830T>A is a newly identified somatic mutation. Protein structure prediction showed that S209T lied in the second transmembrane domain, a conservation region of KCNJ5. S209 was also the phosphorylation site of PKC that is located in intracellular area.

CONCLUSIONMissense mutations of KCNJ5 gene may be associated with UAH. Protein structure prediction has suggested that KCNJ5 mutations may be associated with UAH.

Adrenal Hyperplasia, Congenital ; genetics ; Adult ; Aged ; Amino Acid Sequence ; Female ; G Protein-Coupled Inwardly-Rectifying Potassium Channels ; genetics ; Humans ; Male ; Middle Aged ; Molecular Sequence Data ; Mutation, Missense ; Sequence Analysis, DNA