【Objective】To observe the effect of acupoint external application of herbal drugs on pathological changes of nasal mucosa of allergic rhinitis mice. 【Methods】 Sixty BALB/c mice were divided into 5 groups: A(normal),B(model),C(treated with external application of herbal drugs on acupoints),D(treated with dexamethasone) and E(treated with PBS).Except group A,the mice in other groups were sensitized by ovalbumin(OVA).On the 15~(th) of the first sensitization,group C received external application of medicinal disc(mainly composed of Semen Sinapis, Rhizoma Corydalis,Herba Asari,Radix Kansui,Fructus Xanthii,etc.) on Dazhui (GV14)point,group D received intraperitoneal injection of 0.1?g/L dexamethasone(0.5?mL for each mouse) one hour before nasal dripping of OVA solution,and group E received external application of paper disc on Dazhui point and intraperitoneal injection of(0.1?g/L) PBS(0.5 mL for each mouse) one hour before nasal dripping of OVA solution,qd,for 4 weeks.After treatment,the mice were executed and the nasal mucosa was fixed in 4% paraformaldehyde.Routine paraffin slices were prepared and stained by haematoxylin and eosin.The pathological features of nasal mucosa were observed under light microscope,and eosinophil(EOS),neutrophil and lymphocyte count was performed in different groups.【Results】Compared to group A,the infiltration of EOS and neutrophil in nasal mucosa was obvious in group B(P0.05).【Conclusion】The infiltration of EOS in nasal mucosa plays an important role in the pathogenesis of allergic rhinitis and the decrease of EOS infiltration may be one of the therapeutic mechanism of external application of herbal drugs on acupoints for allergic rhinitis.

Objective:To observe neurotransmitter changes of post cerebral infarction depression (PCID) before and after being treated with amitriptyline or acupuncture of Baihui,in order to explore a good way with a less toxicity and good effect of treatment on PCID.Methods:The 60 patients were divided into treatment group and control group.Besides the basic treatment,the treatment group was given acupuncture of Baihui (DV20) and point injection therapy,the control group was given amitriptyline. Both before and after treatment,compared the two groups with HAMD score,neurologic impairment score,plasma 5-HT and NE,and observed the adverse events and side effects.Results:The results of two groups in monoamine neurotransmitter 5-HT(5-HT),norepinephrine (NE) and the HAMD scale were significantly improved.Conclusion:Acupunture of Baihui and point injection of Herba Erigerontis had equal effect to the amitriptyline on PCID.Furthermore,it had fewer venenosus side effects.It provided a new way for the use of the traditional Chinese medicine in treating PICD.

Objective: To observe effective of antitumor immunity elicited by heat shock protein70(HSP70) associated peptide complexes isolated from hepatoma (HCaF), a tumor model of no MHC Ⅰmolecule expression in mice. Methods: Specific active immunization and adoptive cellular immunization assay was adopted to observe the immunoprotective effect elicited by HSP70 associated peptide complexes purfied from the HCaF. Results: Mice immunized with HSP70 associated peptide complexes were protected from HCaF challenge. The immunoprotective effect in female mice was better than the male groups. Adoptively transferred immune spleen cells of mice which had been immunized with HSP70 associated peptide complexes could be elicited immunity against HCaF challenge, and the mice free of tumor can resist repeated challenge, the maximal challenge quantities exceeded 1?10 7 HCaF cells. Mice immunized once with spleen cells pulsed by HSP70 associated peptide complexes could also be elicited certain immunity against HCaF challenge. Conclusions: HSP70 associated peptide complexes derived from the HCaF can elicit no MHC Ⅰ molecule restrictive immunoprotective effect against HCaF and could be continuously enhanced by repeated challenge with alive HCaF cells. Adoptively transferred immune spleen cells of mice which had been immunized with HSP70 associated peptide complexes could provide protection against HCaF cells challenge.

OBJECTIVE:To upgrade the management of the out-patient pharmacy and provide all the patients, doctors and nurses with good service. METHODS: HIS was applied to the real stock management of the out-patient pharmacy in our hospital. RESULTS: Due to the HIS, the amount of drugs was identical with the account; the overstock and stocktaking error rate were greatly decreased, and the account was clear & the supervision was easy. CONCLUSION: The work efficiency and quality of the out-patient pharmacy was greatly improved by the scientific normalized management.

0.05).In groups A and B,the intraoperative blood loss was(94.4?51.6)ml and(155.8?84.3)ml;the 4% mannitol solution used for intraoperative irrigation was(18.4?6.2)L and(25.4?8.8)L;the operative time was(65.0?16.4)min and((86.8?)25.0)min;the time for postoperative bladder infusion was(46.5?9.1)h and(57.8?17.4)h;the infused saline volume was(19.2?4.2)L and(26.7?10.2)L;the degree of satisfaction of the surgeons with the TURP field was 75.0%(36/48) and 41.9%(26/62);the cases who needed to increase the perfusion pressure during TURP accounted for 22.9%(11/48) and 45.2%(28/62);the blood transfusion rates were 6.2%(3/48) and 22.6%(14/62);and the incidence rates of secondary prostatic bleeding were(10.4)%(5/48) and 25.8%(16/62),respectively.The differences in all these parameters were statistically significant between the 2 groups(P

Objective To report the diagnosis and treatment of an imported case of schistosomiasis haematobium,including the pathological features of the disease and therapeutic efficacy of praziquantel. Methods The data of the patient with schistoso?miasis haematobium were collected,and the pathological features of the bladder tissue were observed under a microscope. More?over,the patient was treated with praziquantel,and his urine was collected before and after the treatment. The eggs in the urine were examined by a microscope after sediment and the miracidia were hatched. Results The patient once worked in Angola for three months,and after returning home he had the symptoms of intermittent painless terminal hematuresis. It was ineffective af?ter anti?inflammatory treatment in a number of hospitals. There were no sand spots discovered under the cystoscope. However , the inflammatory reaction to parasite with a lot of eosinophils infiltration in the bladder mucosa was found on the pathological sec?tions under a microscope,and the egg structure was observed with individual characteristics. The eggs were detected in the urine and the miracidia were hatched before the praziquantel treatment. The hematuria symptoms disappeared after the praziquantel treatment. The eggs were still detected in the urine 7 days post?treatment,but the miracidium could not be hatched. One month and 6 months post?treatment,the eggs were not detected in the urine. Conclusions The imported cases of schistosomiasis hae?matobium are often misdiagnosed,and therefore,it is necessary to strength the health education to the workers overseas and also to improve the ability of diagnosis in medical staff. For the case reported in this paper,there are typical structure of Schistosoma haematobium eggs and egg?granulomas on the pathological sections of bladder tissues. Praziquantel has satisfactory treatment re?sults.

Objective To observe and analyze the changes of inflammatory cytokines levels in patients with progressive cerebral infarction (PCI ).Methods The patients with progressive cerebral infarction (progressive cerebral infarction group),the patients with non-progressive cerebral infarction(non progressive cerebral infarction group)and the healthy individuals(control group)were selected as the research subjects.Sixty individuals were included in each group.The levels of IL-8,hs -CRP,SAA of the research subjects were detected and observed. Results The levels of IL -8[(201.89 ±46.41)mg/L],hs -CRP[(16.55 ±4.49)mg/L],SAA[(20.65 ± 4.17 )mg/L]of the progressive cerebral infarction group were significantly higher than those of non -progressive cerebral infarction group[(132.02 ±40.26 )mg/L,(8.09 ±4.06)mg/L and (15.35 ±4.07 )mg/L).]The differ-ences were statistically significant (t=4.289,P=0.000;t=2.276,P=0.006 and t=2.269,P=0.031).Compared with the control group[(50.70 ±31.14 )mg/L,(0.70 ±0.11 )mg/L and (5.15 ±2.21)mg/L],the levels of IL-8, hs-CRP,SAA in the progressive cerebral infarction group and non -progressive cerebral infarction group were significantly higher on the first day (PCI group vs control:t=3.614,P=0.000;t=3.406,P=0.007;t=2.559, P=0.009;NPCI group vs control:t=3.602,P=0.000;t=3.181,P=0.009;t=1.520,P=0.026).Conclusion The changes of the inflammatory factors levels such as IL-8,hs-CRP,SAA have certain predicative value on the development of the PCI.

Purpose　To investigate relationship between androgen receptor and apoptotic index and to further understand the tumor biology of prostate cancer. Methods　Fifty-six patients with histologically proven prostate cancer and 20 cases with benign prostatic hyperplasia(BPH) were collected. Androgen receptor(AR) were stained by 2H12 monoclonal antibody using immunohistochemical method. The apoptotic index (AI) was determined by the terminal deoxynucleotidyl transterase-mediated dUTP biotin nick end labeling (TUNEL) technique on serial sections of formalin fixed, paraffin embedded tissues. Results　AR was not found significantly difference (P>0.05) between prostate cancer and BPH. The association of AR with AI according to Gleason score were not observed in prostate cancer. AI were significantly higher in prostate cancer compared to BPH (P<0.05).AI were also significantly higher in AR-positive prostate cancer than in AR-negative prostate cancer（P<0.05）. Conclusion　AR expression can induce prostate cancer cells to become apoptosis, and may be a useful predictor in functional classification and endocrine response of prostate cancer.

A total of 206 patients of type 2 diabetes mellitus (T2DM) were assigned into the trial group (n =156) and the control group (n =50) according to whether real object-based diabetic diet was provided.Traditional nutritional education for both groups during hospitalization.The patients in the trial group received a real object-based diabetic diet guided by a nutritionist who adjusted feeding regimens and skills before and after operation.The control group had a free access to a normal diet.The patient understanding of knowledge,attitude and practice (KAP) was examined by nutrition surveys.And their postoperative nutrition states and complications were assessed by questionnaires during a 6-month follow-up.The total average score of cognitive divided was 93.4 ± 7.5,the average score of diet therapy knowledge 13.8 ± 1.6 and the average scores of attitude and practice 9.6 ± 1.9 and 32.2 ±3.9.Significant differences existed between two groups (P ＜ 0.05).The incidence rates of hypoglycemia,hyperglycemia and gastrointestinal obstruction were lower than the control group (P ＜ 0.05,P ＜ 0.01).It suggested that real object-based diabetic diet guidance plus traditional nutrition education could improve the KAP level and may lower the incidence of postoperative complications in T2DM patients with gastric bypass.

Small animal PET is a quantitative imaging technique that can noninvasive and dynamically image the distribution of positron-labeled radiopharmaceuticals in vivo,therefore representing a new means of providing information for drug development and evaluation.This article reviews the fundamental basis of PET imaging and their application in preclinical drug discovery and development.