Objective To investigate the quality of life (QOL) and its main influencing factors in patients with inflammatory bowel disease (IBD) and their families members in Fujian Province,and to provide an evidence for guiding the clinical strategy.Methods From September 2015 to March 2016,114 IBD patients who met the standards,their 97 family members,and 113 irritable bowel syndrome (IBS) patients were enrolled.The QOL was assessed with 36-item short form health survey questionnaire (SF-36).T test,inspection rank and Chi-square were performed for statistical analysis in two groups and multiple group.The influence factors of QOL using multiple linear stepwise regression analysis.Results There was no difference in QOL scores between CD and UC patients ((62.82 ± 18.55) vs (62.60 ± 17.94);t=0.065,P=0.948).The QOL scores of IBD patients were lower than those of IBS patients (62.72± 18.18 vs 67.25 ± 16.26),and the difference was statistically significant (t=-1.975,P=0.049).The QOL scores of IBD patients with medical insurance were higher than those of patients without medical insurance (67.13 ±18.18 vs 57.06 ± 16.68),and the difference was statistically significant (t=3.034,P=0.003).The dimension scores of body pain,general health,social function,emotional function,mental health in IBD patients relatives were lower than those of the general population ((72.29±17.27) vs (85.61±18.37),(60.92±17.08) vs (69.55±21.32),(80.80±15.32) vs (86.85±17.07),(68.38±37.38) vs (76.45±38.45),(61.97±15.30) vs (72.65 ± 16.81),respectively),and the differences were statistically significant (t=-7.597,-4.979,-1.870,-2.125,-6.875,all P＜0.05).The total QOL scores of families members of IBD patients with operation history was lower than those of without operation history (64.62± 16.74 vs 73.98±14.15),and the difference was statistically significant (t=-2.540,P=0.013).Conclusions The QOL of IBD patients was lower than that of IBS patients,and also The QOL relates with payment types for medical care,and the QOL of family members of IBD patients also decreases.Psychological interventions should be in plemented in both IBD patients and their family members in clinical treatment.

Objective To investigate the expression of human leucocyte antigen-G (HLA-G) in the endometrium of patients with endometriosis (EM). Methods The expression of HLA-G protein was detected in ovarian endometriosis (OEM) and adenomyosis (AM) using immunohistochemistry method, and the expression of HLA-G mRNA was detected using in situ hybridization technique (ISH), to compare with that in the endometrium of hysteromyoma as the control group. Results The rate of positive HLA-G expression in EM (including AM and OEM) was significantly higher than that in control group (P0.05). The expression of HLA-G protein showed no relation with different clinical phase or different endometrial cycle. Conclusion HLA-G is overexpressed in EM, which may play certain role in the pathogenesis of the disease.

Objective To explore the regulatory effects of thioredoxin on chemosensitivity of gastric cancer cells. Methods RT-PCR was employed to clone the full-length cDNA of thioredoxin (Trx) from human drug-resistant gastric cancer cell line SGC7901/VCR. The sense and antisense eukaryotic expression vectors corresponding Trx were constructed and transduced into gastric cancer cells SGC7901 and SGC7901/VCR by lipofectamine, respectively. MTT assay was used to evaluate the sensitivity of gene-transfected gastric cancer cells and control cells to anti-cancer drugs in vitro. Results The results indicated that SGC7901 cells transfected with Trx sense vector showed significantly decreased sensitivity to vincristine, cisplatin and adriamycin, while SGC7901/VCR transfected with Trx antisense vector showed significantly increased sensitivity to these drugs. Conclusion All these data suggested that Trx might participate in the formation of multidrug resistance of gastric cancer cells and alteration of Trx could regulate chemosensitivity of gastric cancer cells.

OBJECTIVE:To provide references for the perfecting of Chinese administration system on retail pharmacies.METHODS:The related laws and regulations of the supervising institutions of retail pharmacies in the developed countries like America and Great Britain were collected,compared and analyzed.RESULTS:The supervision systems of retail pharmacies in the developed countries like America and Great Britain have noticeable merits and rationalities.CONCLUSION:The construction of supervising system and the arrangement of its authorities on retail pharmacy in countries like America,Great Britain have some reference value for the supervision of Chinese retail pharmacies in its way towards more rationality and humanity.

ObjectiveTo observe the effects of local mild hypothermia at different time on dopamine (DA) and 5-hydroxytryptamine (5-HT) concentration in rabbits brain basal ganglion with bilirubin encephalopathy. MethodsThe model of bilirubin encephalopathy was established, and treated with mild hypothermia for different time. The DA and 5-HT in the basal ganglion were detected with fluorescence spectrophotometry. ResultsThe DA and the 5-HT decreased in the basal ganglion significantly after modeling (P<0.01), and increased when treated for 6 h, 12 h or 24 h (P<0.01). ConclusionLocal mild hypothermia can significantly reduce the disorder of DA and 5-HT during bilirubin encephalopathy. The longer the hypothermia, the better the effect is.

Objective:To approach the actions of survivin and COX-2 during the genesis and development in brain astrocytoma and whether they have dependablity each other.Methods:The expression of survivin and COX-2 was examined by immunohistochemistry SP and their interrelationship was analyzed.Results:The positive expressive rates of survivin in astrocytoma gradeⅠ,Ⅱ,Ⅲ and Ⅳsubgroups were shown in an increased tendency.The expression was weak positive in higher differentiation group,and the positive expressive rate of survivin was increased in the lower differentiation group.There was an significant difference between these two groups(?2=11.20,P

Objective To investigate the realationship between resting heart rate(RHR) and carotid arterial intima-media thickness(IMT)in senile patients with essential hypertension.Methods All 164 elderly essential hypertensive patients were divided into three groups according to the levels of blood pressure(BP): the first group,140/90 mmHg≤SBP

Objective To explore the clinical significance of urinary ?1-microglobulin (?1-MG), ?2-micro-globulin (?2-MG), albumin (MA), immunoglobulin G (IGU) and transferritin (TRU), and investigate the correlation between microproteinuria and renal pathological changes in patients with systemic lupus erythematosus (SLE). Methods According to the presence of nephritis, 47 SLE patients who had renal biopsy were divided into two groups: 30 patients with clinical lupus nephritis (OLN), and 17 patient with silent lupus nephritis (SLN). The urinary ?1-MG, ?2-MG, MA, IGU and TRU were measured in SLE patients and compared with healthy control group. The level of microproteinuria and renal biopsy pathological changes in SLE patients were studied. Results ①The levels of urinary ?1-MG, ?2-MG, MA, IGU and TRU had statistical differences between SLE group and healthy control group, OLN group and SLN group, SLN group and healthy control group respectively. ②The levels of urinary MA、TRU、 IGU were significantly higher in WHO class Ⅳ than in class Ⅱ LN, the level of urinary MA was markedly increased in class Ⅳ than in class Ⅲ LN and ?1-MG was significantly different between class Ⅳ and Ⅴ LN. In classⅤ LN, the urinary MA was obviously increased compared with class Ⅱ LN. Conclusion ①The urinary ?1-MG, ?2-MG, MA, IGU and TRU reflects the severity of renal damage in SLE patients and may be used as sensitive markers for early lupus nephritis. ②The measurement of microproteinuria indicates that the difference in severity of renal damage exists among different WHO classes of LN and urinary MA and ?1-MG may be sensitive indicators.

BACKGROUND: Prognosis is determined by degrees of outside force and time of spinal cord compression after spinal cord injury. The former factor cannot be changed, but the latter one can be changed through relieving spinal cord compression as early as possible to promote nervous function recovery.OBJECTIVE: To compare the effect of surgical intervention time on nervous function recovery within 72 hours and 10 to 14 days after cervical spinal cord injury.DESIGN: Randomized controlled and before-after controlled study.SETTING: Department of Spine Surgery of the Second Hospital affiliated to Harbin Medical University.PARTICIPANTS: A total of 32 patients with cervical spinal cord injury were selected from the Department of Spine Surgery of the Second Hospital affiliated to Harbin Medical University from April 1998 to August 2001.All patients were divided into two groups according to randomly alternative criteria. There were 16 cases including 10 males and 6 females in early surgical group (within 72 hours) and there were also 16 cases including 12males and 4 females in delayed surgical group (within 10-14 days).METHODS: Patients in early surgical group were undertaken operation at 72 hours after hospitalization, and patients in delayed surgical group were at 10-14 days after hospitalization. Frankel grade, sensory and motor scores were recorded according to criteria set by American Spinal Injury Association before operation and within 24 months after operation.MAIN OUTCOME MEASURES: ① Sensory and motor scores before and after operation; ② Frankel grade before and after operation.RESULTS: ① Improved level of sensory score (difference before and after operation) was higher in early surgical group than that in delayed surgical group (42.6±20.2, 19.2±19.1, P ＜ 0.01). ② Improved level of motor score (difference before and after operation) was higher in early surgical group than that in delayed surgical group (39.7±17.8, 17.3±18.6, P ＜ 0.01). ③Improved level of Frankel grade in early surgical group was superior to that in delayed surgical group (P ＜ 0.01).CONCLUSION: Nervous function recovery of patients with acute cervical spinal cord injury who were suffered from operation within 72 hours is superior to that of those within 10 to 14 days. Therefore, surgical intervention should be undertaken as early as possible in order to promote nervous function recovery after cervical spinal cord injury.

Objective To get the protein expression of sFlt-1 by transfection and to investigate the effects of sFlt-1 gene transfection on the growth of K562 cells.Methods The recombinant plasmid pcDNA3-sFlt-1D4 was constructed.The recombinant plasmid pcDNA3-sFlt-1D4 was transfected into the bone marrow stromal cells by Lipofectamine 2000,which was identified by RT-PCR,ELISA and MTT.Results The transfection efficiency identified by flow cytometry was 9.27%.The protein expression of sFlt-1D4 was found in the culture supernatant 24 h,48 h and 4 weeks after transfetion by ELISA and the expression concentrations were(0.104?0.078),(0.158?0.022) and(0.171?0.069) ?g?L-1,respectively.The content of VEGF secreted by K562 culturing with transfectant cells culture supernatant was reduced compared with control.The inhibitoy rates on the proliferation of K562 cells via MTT assay were 9.41%?4.71%,23.63%?7.50%,and 33.13%?6.93%,respectively.Conclusion The bone marrow stromal cells transfected with recombinant plasmid pcDNA3-sFlt-1D4 could secrete sFlt-1D4 and inhibit the proliferation of K562 cells.