1.Application of the Cerebral Palsy Rehabilitation System in the Digital Community on the Thumb Adduction Deformity
Yanqiong DAI ; Li CHEN ; Huiyu ZHOU ; Xiuhua CHEN ; Yuanfei SHAN ; Liang TANG
Progress in Modern Biomedicine 2017;17(22):4250-4253
Objective:To explore the effect of digital rehabilitation system on the recovery of infants with cerebral palsy.Methods:Twenty-one children with cerebral palsy were treated with residual cerebral palsy in Putuo District,and 21 children with cerebral palsy were followed up.The patients were divided into two groups (n =21).The control group was treated by routine OT training by the parents,and the treatment group was treated with the digital rehabilitation system.Three months later,the efficacy was evaluated and compared.Results:After 3 months of treatment,the total effective rate (effective rate and effective rate) of the two groups was 90.5% and 81%,respectively,and the treatment group was significantly higher than the control group (P <0.05).The PROM of the two groups was improved (P <0.01),and the PROM in the treatment group was significantly higher than that in the control group (P <0.05).FMFM was significantly higher than that before treatment (P <0.01),and FMFM was significantly higher in the treatment group than in the control group (P <0.01).Conclusion:Family (community) digital rehabilitation system can effectively improve the rehabilitation of children with cerebral palsy.
2.Influence of HMGB1/MAPK/m-TOR signaling pathway on cell autophagy and chemotherapy resistance in K562 cells.
Liying LIU ; Fei GAO ; Yanqiong YE ; Zhiheng CHEN ; Yunpeng DAI ; Ping ZHAO ; Guotao GUAN ; Mingyi ZHAO
Journal of Central South University(Medical Sciences) 2016;41(10):1016-1023
To observe the effect of high-mobility group box 1 (HMGB1) on autophagy and chemotherapy resistance in human leukemiacell line (K562) cells, and to explore the underlying mechanisms.
Methods: The K562 cells were cultured in vitro and divided into 6 groups: a chemotherapeutic group, a chemotherapeutic control group, a HMGB1 preconditioning group, a HMGB1 preconditioning control group, a HMGB1 siRNA group and a siRNA control group. The chemotherapeutic group was further divided into a vincristine (VCR) group, an etoposide (VP-16) group, a cytosine arabinoside (Ara-C) group, a adriamycin (ADM) group and a arsenic trioxide (As2O3) group. The cell activity was evaluated by cell counting kit-8. The protein levels of HMGB1, microtubule-associate protein1light chain3 (LC3), AMP-activated protein kinase (AMPK) and mammalian target of rapamycin (m-TOR) were determined by Western blotting. The level of serum HMGB1 was evaluated by enzyme-linked immunosorbent assay (ELISA). The autophagy was examined by monodansylcadaverine staining and observed under transmission electron microscopy.
Results: Compared with the control group, the cell activity was significantly decreased and the level of serum HMGB1 was significantly increased in the chemotherapeutic (VCR, VP-16, Ara-C, ADM and As2O3) groups (all P<0.05). Compared with the control group, the cell activity and the level of serum HMGB1 were significantly increased in the HMGB1 preconditioning group (both P<0.05). Compared with the siRNA control group, the cell activity and the level of serum HMGB1 were significantly decreased in the HMGB1 siRNA group (both P<0.05). Compared with the control group, the expression of LC3-II and the formation of autophagic bodies were increased in the HMGB1 preconditioning group (both P<0.05), the p-AMPK expression was increased and p-mTOR expression was decreased (both P<0.05).
Conclusion: HMGB1 can increase the autophagy and promote chemotherapy resistance through the pathway of AMPK/m-TOR in K562 cells.
AMP-Activated Protein Kinases
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genetics
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physiology
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Arsenic Trioxide
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Arsenicals
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Autophagy
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genetics
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Cytarabine
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Doxorubicin
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Drug Resistance, Neoplasm
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genetics
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physiology
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Etoposide
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HMGB1 Protein
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genetics
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physiology
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Humans
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K562 Cells
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physiology
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Microtubule-Associated Proteins
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Oxides
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RNA, Small Interfering
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Signal Transduction
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TOR Serine-Threonine Kinases
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genetics
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physiology
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Vincristine