Objective:To explore the electrocardiogram changes of myocardial ischemia(30 min) and reperfusion(120 min) in rats. Methods:We selected 16 SD rats to set up models of myocardial ischemia and reperfusion,and observed the changes of P complex,QRS complex,T wave,S-T segment and the occurrence of arrhythmia in electrocardiogram of lead Ⅱ. Results: ①12 models were useful.② R waves of the 12 models rose transitorily and abnormally during the occlusion of the left coronary artery;new q waves appeared or original q waves deepened when reperfused for 15-20 min,further more,the q waves broadened and deepened gradually along with the reperfusion.③S-T segment and T wave of the 12 models rose gradually from the beginning of coronary artery occlusion to ischemia 20 min.After that they were relatively stable during the ischemia and descended rapidly after the start of reperfusion.The fall of ST segment and T wave was more than 50% before reperfused for 15 min.④Ventricular arrhythmia appeared in 13 models,which occurred mainly after 5-10 min of the coronary artery occlusion and the start of reperfusion. Conclusion: The results suggest that the gradual elevation of S-T segment and T wave to the peak within 15-20 min of myocardial ischemia can be regarded as the index of coronary artery occlusion of rats.Rapid falling of S-T segment and T wave after the start of reperfusion and over 50% drop within 15 min of reperfusion can be regarded as the index of coronary artery reflow of rats.

A series of 61 patients are presented, among them 38 patients(twenty with cancer of esophagus, two with cancer of cardia, four with cancer of the upper part of st- omach, and radical resection were done in all of them)have been supported by TPN during perioperative and postoperative period, none of leakage at the anastomostic stoma occured; five paients with external fistulas of the alimentary tract supported by TPN healed spontaneously in four, operative closure of fistula was necessary in one, and all were cured. TPN was used in other 18 patients with miscellaneous disease, among them two of the patients with acute haemorrhagic pancreatitis were cured.One patient with major nutritional problem is presented in detail, for whom TPN had been continued for 225 days.

Objective To study the distribution of purinergic P2X_3 receptor immunoreactive afferent fibers in the rat pharyngeal mucosa and provide morphological evidence for exploring the role of ATP in the signal transduction of sensory stimuli. Methods Twelve adult Wistar rats were used and immunofluorescenthistochemical double-labeling technique combined laser confocal scanning microscope was applied in the present study. Results 1.P2X_3 immunoreactive fibers were observed on sections from all parts of pharyngeal mucosa. Two types of positive fibers were found. One was free nerve fibers covered with many varicosities. Another one ramified in mucosa and showed complex arborization endings. Nerve plexus in mucosa were formed by P2X_3 immunoreactive fiber ramifications. 2.Most calcitonin gene-related peptide (CGRP) positive fibers intermingled with P2X_3 immunoreactive fiber arborizations. A few P2X_3 immunoreactive fibers were covered with many varicosities co-localized with CGRP. 3.In petrosal ganglion, most neurons were stained with P2X_3 or CGRP immunoreactivity and small number of P2X_3 immunoreactive neurons co-stained with CGRP. Conclusion These results indicated that the different types of afferent fibers in rat pharyngeal mucosa expressed purinergic P2X_3 receptor immunoreactivity and ATP might be related to nociceptive or physiological signals transduction in rat pharyngeal mucosa.

Medical humanistic education becomes “remarkable leaning” because many problems arise from the development of contemporary medicine such as the meaning of life,the medical aim,the medical model and doctor-patient relationship etc.Multi-channel and multi-modal development of medical humanistic education has become a must to understand medicine and makes it develope soundly,and all-directional resultant force and the environment of medical humanistic education,striving for studies,is an exploration and even a mission and responsibility

The diagnosis of Spitz nevus is mainly based on histopathological changes. Combined with the learning experience at Ackerman Academy of Dermatopathology, the author thoroughly analyzed pathological and cytological characteristics of, as well as the body′s reaction to Spitz nevus, hoping to help clinicians with the pathological diagnosis and differential diagnosis of Spitz nevus.

Objective To observe the effect of Fuzheng Hunao capsule on TNF-? and HSP70 protein expression of myocardial tissues in rats with intracerebral hemorrhage.Methods Seventy two rats were randomly divided into 4 groups:sham,model,Fuzheng Hunao and Angong Niuhuang.The protein expression of TNF-? and HSP70 in rat myocardial tissues were detected by the immunohistochemistiy method,and the integrated optical density (IOD) was measured with image analysis.Result The IOD of TNF-? and HSP70 in model group was higher than that in sham group.The IOD of TNF-? in Fuzheng Hunao and Angong Niuhuang group was lower than that in model group.While the IOD of HSP70 was higher than that in model group.Conclusion Inflammatory response attend the myocardial damage.Fuzheng Hunao capsule could protect the myocardial tissues by inhibiting the protein expression of TNF-? and increasing that of HSP70.

Abstracts Experience in total parenteral nutrition (TPN) support for 27 patients with acute severe panereatitis is presented. Most patients were seriously ill, and the course were long and complicated with a mean duration of 64 days, and the longest one was 179 days. Metabolic disturbance and malnutrition were very conspicuous. The mean duration of TPN support for each patient was 24 days, and the longest one maintained for 72 days. TPN began from the early stage and cover the whole period of alimentary dysfunction until the patient could take food by mouth in most patients. Combined treatment was important, ineluding: early operation in 19 patients, intra-and/or postoperative lavage of pancreatic bed and and peritoneal cavity in 13 patients, and as well as appropriated conservative management. Late operative drainage for pancreatic abscess was done in 7 cases. Results Were satisfactory with 22 (81. 5% ) were cured and 5 (18. 5% ) died. In the latter, 3 were died of pancreatic sepsis complicated with infective shock, 2 died of exhaustion, together with pneumonia and multiple system organ filure. None of early died.

Objective:To evaluate the influence on root resorption after distalizing molars with face bow.Methods:15 cases whose molars were distalized with face bow were selected in present research. Root lengths were measured on pre-treatment and post-treatment dental films taken with the oral positioner. The distances of molar distalization were measured on cephalometric radiographs.The differences of the pre-treatment and post-treatment root lengths were evaluated by the Paired t-test.The correlation between the distance of molars distalization and the root resorption of the maxillary molars was analyzed with Pearson's correlation coefficients.Results:The root lengths between the pre-treatment and post-treatment showed a statistically significant difference(P

AIM:To explore clinical effect of Zhongfeng Fangzhiling Granule(Radix Pseudostellariae,Radix Polygoni Multiflori praeparata cum succo glycines sotae,Hirudo,etc.) on acute cerebral thrombosis.METHODS:72 patients were randomly divided into 2 groups.The treatment group was treated with Zhongfeng Fangzhiling Granule,the control group was treated with Zhilong Xuetong Capsule.The treatment effects between two groups were compared.RESULTS:The effect in treatment group was superior to that in control group.CONCLUSION:Zhongfeng Fangzhiling Granule has significiant therapeutic effect on patients with acute cerebral thrombosis,and the preparation method is simple,so it will be a prospective herb granule.

Objective To evaluate the biological characteristics of gastric carcinoma and non cancer gastric mucosa which might develop to cancer.Methods The DNA content and proliferative activity of cells in 154 patients with primary mucosal gastric cancer and 40 patients with gastric ulcer wese detected by flow cytometric analysis. A total of 154 samples from mucosal gastric carcinomas, 154 samples from normal mucosa adjacent to carcinomas and 40 samples from normal gastric mucosa adjacent to gastric ulcers were subjected to analysis.Results The incidence of DNA aneuploidy of differentiated carcinoma cells (34.3%) was significantly higher than that of undifferentiated carcinoma cells (14.5%, P